Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab23

Expression vector of mouse Rab23 fused with EGFP.

Sequence (full)	RDB17269zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17269_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert Sequence file: RDB17269_A9Ela.seq
>D06280F6_A9El_1_EGFP-C2_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNCGNN CNNNNNNTCC TGCTGGNNTT CGTGNNCGCC GCCGGGNTCA    61 CTCTCGGCAT GGNCGAGCTG TACAAGTCCG GACTCAGATC CATGTTGGAG GAAGATATGG   121 AAGTGGCCAT CAAGATGGTG GTGGTGGGGA ATGGGGCGGT TGGAAAGTCG AGCATGATTC   181 AGCGCTACTG CAAAGGCATT TTCACAAAAG ACTACAAGAA AACCATCGGC GTAGACTTTT   241 TGGAGCGACA GATTCAGGTT AACGATGAAG ATGTAAGGCT AATGTTATGG GACACTGCAG   301 GTCAAGAGGA GTTTGATGCA ATCACGAAGG CCTACTATCG AGGAGCCCAG GCTTGTGTGC   361 TTGTGTTTTC TACCACAGAC AGGGAATCTT TTGAAGCGAT TTCCAGCTGG AGAGAAAAAG   421 TAGTAGCGGA GGTTGGAGAT ATACCAACCG CACTGGTGCA AAACAAGATC GATCTGCTGG   481 ATGATTCATG CATCAAAAAT GAGGAAGCGG AGGGACTGGC CAAAAGGCTA AAGCTGAGGT   541 TCTACCGGAC GTCCGTGAAG GAGGACCTCA ACGTGAGTGA AGTTTTTAAA TATTTGGCTG   601 AGAAACACCT ACAAAAACTC AAACAACAAA TAACCGAAGA TCCAGAACAG ACACACTCAA   661 GTAGTAACAA GATTGGTGTC TTTAATGCAT CGGTCGGAAG TCACTTGGGC CAGAATTCAA   721 GTTCCCTTAA TGGTGGCGAT GTCATCAACC TTAGACCTAA CNAACAAAGG ANCAAGAGAA   781 CCAGAAACCC TTTCAGCAGC TGTAGTGTAC NNTAAATCAC TAGTGAANTC GCNGCCGCCT   841 GCANGNCGAC NGNNCCGCNG GCCCGGGNTC CACCGGNTCT AGATAANNGA TCATNATCAG   901 CNNNACCACN TTTGTANANN TTTTNNNNGC TTTNAAAAAA NNNNCNNCAC NNNCCNNNNN 961 NNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert Sequence file: RDB17269_A9Elb.seq
>D06280F6_A9El_1_SV40polyA-R2_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA NNNAAGTTAA CNANNACNAT TGCATTCNTT TTATGTTTCA    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT   181 GCAGGCGGCC GCGAATTCAC TAGTGATTTA GGGTACACTA CAGCTGCTGA AAGGGTTTCT   241 GGTTCTCTTG GTCCTTTGTT TGTTAGGTCT AAGGTTGATG ACATCGCCAC CATTAAGGGA   301 ACTTGAATTC TGGCCCAAGT GACTTCCGAC CGATGCATTA AAGACACCAA TCTTGTTACT   361 ACTTGAGTGT GTCTGTTCTG GATCTTCGGT TATTTGTTGT TTGAGTTTTT GTAGGTGTTT   421 CTCAGCCAAA TATTTAAAAA CTTCACTCAC GTTGAGGTCC TCCTTCACGG ACGTCCGGTA   481 GAACCTCAGC TTTAGCCTTT TGGCCAGTCC CTCCGCTTCC TCATTTTTGA TGCATGAATC   541 ATCCAGCAGA TCGATCTTGT TTTGCACCAG TGCGGTTGGT ATATCTCCAA CCTCCGCTAC   601 TACTTTTTCT CTCCAGCTGG AAATCGCTTC AAAAGANTCC CTGTCTGTGG TAGAAAACAC   661 AAGCACACAA GCCTGGGCTC CTCGATAGTA GGCCTTCGTG NNTGCATCAA ACTCCTCTTG   721 ACCTGCAGTG TCCCATAACA TTAGCCTTAC ATCTTCATCG TTAACCTGAA TCTGTCGCTC   781 CNAAAAGTCT ACGCCGATGG NTTTCTTGTA NTCTTTTGTG AAAATGCCTT TGCAGTANCG   841 CTNAATCATG CTCGANTTTC CNACCGCCCN NTTCCCCACC ACCACNNTCN TGATGGNCAC   901 TTCCNTANNN NCCNNCNNCN NGGNTCTGAN TCNNGANNNN NACANCNCNT CCATNNNNNN 961 NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB17269_A9Elc.seq
>D06280F6_A9El_1_CMV_Forward_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNTTNNNGC NNTGGGCGGT ANGCGTGTAC GGTGGGNNGT CTATATAAGC    61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA   121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT   361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG   601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GANCACTACC   661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA   721 CCCAGTCCGC CCTGNNCNAA GANNNCAACG ANAANCGCGA TCACATGGTC CTGCTGGANT   781 TCGTGACCGC CGCCNGGNTC ACTCTCGGCA TGNNCGANCT GNACAANNCC GGANTCANAT   841 NCNNGTTGGA NGAAGATATN NNANTGGNNN TCNNNATGGN GGNGGNGGGG GNANNNGGGN 901 NNGNNNGGAA ANNCNNNNAT NNNTCNNNNN NNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB17269_A9Eld.seq
>17269_17269_B1JdF_SV40pro_F_V2_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC TTTTGCNNAG ANCGATCAAG AGACAGGNTG AGGNNCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCNAGGC GAGCATGCCC   601 GACGGCGANG NNCTCGTCGT GACCCNTGGC GANGCCTGCN NGNCGAATAT CATGGNGGAA   661 AATGNCCGCT TTTCTGGANT CNTCGNCNGT GGNCGGNNGG GNGNNNNNGN ANNNNNNNCN 721 NNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert 5' Sequence file: RDB17269_A9Ele.seq
>17269_17269_B1JdF_mVenus-M_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA    61 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA   121 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA   181 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA   241 CAAGTCCGGA CTCAGATCCA TGTTGGAGGA AGATATGGAA GTGGCCATCA AGATGGTGGT   301 GGTGGGGAAT GGGGCGGTTG GAAAGTCGAG CATGATTCAG CGCTACTGCA AAGGCATTTT   361 CACAAAAGAC TACAAGAAAA CCATCGGCGT AGACTTTTTG GAGCGACAGA TTCAGGTTAA   421 CGATGAAGAT GTAAGGCTAA TGTTATGGGA CACTGCAGGT CAAGAGGAGT TTGATGCAAT   481 CACGAAGGCC TACTATCGAG GAGCCCAGGC TTGTGTGCTT GTGTTTTCTA CCACAGACAG   541 GGAATCTTTT GAAGCGATTT CCAGCTGGAG AGAAAAAGTA GTAGCGGANG TTGGAGANAT   601 ACCNACCGCA CTGGTGCNAA ACNAGATCGA TCTGCTGGAT GANTCNTGNA TCNAAATGAN   661 GNANCGNANG GNNNNGNCNA AANNNTNANN CTGNNNNCNN NNNNNNNTNN NNGNNNNNNN 721 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl