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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab23 (#RDB17269)

Expression vector of mouse Rab23 fused with EGFP.

Clone info. Expression vector of mouse Rab23 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab23 cDNA
Reference of insert sequence AB232618 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB17269zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

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RDB17269 pEGFP-C1-Rab23 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab23 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17269).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17269_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert
Sequence file: RDB17269_A9Ela.seq check
>D06280F6_A9El_1_EGFP-C2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN CNNNNNNTCC TGCTGGNNTT CGTGNNCGCC GCCGGGNTCA
   61 CTCTCGGCAT GGNCGAGCTG TACAAGTCCG GACTCAGATC CATGTTGGAG GAAGATATGG
  121 AAGTGGCCAT CAAGATGGTG GTGGTGGGGA ATGGGGCGGT TGGAAAGTCG AGCATGATTC
  181 AGCGCTACTG CAAAGGCATT TTCACAAAAG ACTACAAGAA AACCATCGGC GTAGACTTTT
  241 TGGAGCGACA GATTCAGGTT AACGATGAAG ATGTAAGGCT AATGTTATGG GACACTGCAG
  301 GTCAAGAGGA GTTTGATGCA ATCACGAAGG CCTACTATCG AGGAGCCCAG GCTTGTGTGC
  361 TTGTGTTTTC TACCACAGAC AGGGAATCTT TTGAAGCGAT TTCCAGCTGG AGAGAAAAAG
  421 TAGTAGCGGA GGTTGGAGAT ATACCAACCG CACTGGTGCA AAACAAGATC GATCTGCTGG
  481 ATGATTCATG CATCAAAAAT GAGGAAGCGG AGGGACTGGC CAAAAGGCTA AAGCTGAGGT
  541 TCTACCGGAC GTCCGTGAAG GAGGACCTCA ACGTGAGTGA AGTTTTTAAA TATTTGGCTG
  601 AGAAACACCT ACAAAAACTC AAACAACAAA TAACCGAAGA TCCAGAACAG ACACACTCAA
  661 GTAGTAACAA GATTGGTGTC TTTAATGCAT CGGTCGGAAG TCACTTGGGC CAGAATTCAA
  721 GTTCCCTTAA TGGTGGCGAT GTCATCAACC TTAGACCTAA CNAACAAAGG ANCAAGAGAA
  781 CCAGAAACCC TTTCAGCAGC TGTAGTGTAC NNTAAATCAC TAGTGAANTC GCNGCCGCCT
  841 GCANGNCGAC NGNNCCGCNG GCCCGGGNTC CACCGGNTCT AGATAANNGA TCATNATCAG
  901 CNNNACCACN TTTGTANANN TTTTNNNNGC TTTNAAAAAA NNNNCNNCAC NNNCCNNNNN
961 NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert
Sequence file: RDB17269_A9Elb.seq check
>D06280F6_A9El_1_SV40polyA-R2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNAAGTTAA CNANNACNAT TGCATTCNTT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT
  181 GCAGGCGGCC GCGAATTCAC TAGTGATTTA GGGTACACTA CAGCTGCTGA AAGGGTTTCT
  241 GGTTCTCTTG GTCCTTTGTT TGTTAGGTCT AAGGTTGATG ACATCGCCAC CATTAAGGGA
  301 ACTTGAATTC TGGCCCAAGT GACTTCCGAC CGATGCATTA AAGACACCAA TCTTGTTACT
  361 ACTTGAGTGT GTCTGTTCTG GATCTTCGGT TATTTGTTGT TTGAGTTTTT GTAGGTGTTT
  421 CTCAGCCAAA TATTTAAAAA CTTCACTCAC GTTGAGGTCC TCCTTCACGG ACGTCCGGTA
  481 GAACCTCAGC TTTAGCCTTT TGGCCAGTCC CTCCGCTTCC TCATTTTTGA TGCATGAATC
  541 ATCCAGCAGA TCGATCTTGT TTTGCACCAG TGCGGTTGGT ATATCTCCAA CCTCCGCTAC
  601 TACTTTTTCT CTCCAGCTGG AAATCGCTTC AAAAGANTCC CTGTCTGTGG TAGAAAACAC
  661 AAGCACACAA GCCTGGGCTC CTCGATAGTA GGCCTTCGTG NNTGCATCAA ACTCCTCTTG
  721 ACCTGCAGTG TCCCATAACA TTAGCCTTAC ATCTTCATCG TTAACCTGAA TCTGTCGCTC
  781 CNAAAAGTCT ACGCCGATGG NTTTCTTGTA NTCTTTTGTG AAAATGCCTT TGCAGTANCG
  841 CTNAATCATG CTCGANTTTC CNACCGCCCN NTTCCCCACC ACCACNNTCN TGATGGNCAC
  901 TTCCNTANNN NCCNNCNNCN NGGNTCTGAN TCNNGANNNN NACANCNCNT CCATNNNNNN
961 NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17269_A9Elc.seq check
>D06280F6_A9El_1_CMV_Forward_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNTTNNNGC NNTGGGCGGT ANGCGTGTAC GGTGGGNNGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GANCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA
  721 CCCAGTCCGC CCTGNNCNAA GANNNCAACG ANAANCGCGA TCACATGGTC CTGCTGGANT
  781 TCGTGACCGC CGCCNGGNTC ACTCTCGGCA TGNNCGANCT GNACAANNCC GGANTCANAT
  841 NCNNGTTGGA NGAAGATATN NNANTGGNNN TCNNNATGGN GGNGGNGGGG GNANNNGGGN
901 NNGNNNGGAA ANNCNNNNAT NNNTCNNNNN NNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17269_A9Eld.seq check
>17269_17269_B1JdF_SV40pro_F_V2_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC TTTTGCNNAG ANCGATCAAG AGACAGGNTG AGGNNCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCNAGGC GAGCATGCCC
  601 GACGGCGANG NNCTCGTCGT GACCCNTGGC GANGCCTGCN NGNCGAATAT CATGGNGGAA
  661 AATGNCCGCT TTTCTGGANT CNTCGNCNGT GGNCGGNNGG GNGNNNNNGN ANNNNNNNCN
721 NNNNNNNNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'
Sequence file: RDB17269_A9Ele.seq check
>17269_17269_B1JdF_mVenus-M_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
   61 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
  121 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
  181 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
  241 CAAGTCCGGA CTCAGATCCA TGTTGGAGGA AGATATGGAA GTGGCCATCA AGATGGTGGT
  301 GGTGGGGAAT GGGGCGGTTG GAAAGTCGAG CATGATTCAG CGCTACTGCA AAGGCATTTT
  361 CACAAAAGAC TACAAGAAAA CCATCGGCGT AGACTTTTTG GAGCGACAGA TTCAGGTTAA
  421 CGATGAAGAT GTAAGGCTAA TGTTATGGGA CACTGCAGGT CAAGAGGAGT TTGATGCAAT
  481 CACGAAGGCC TACTATCGAG GAGCCCAGGC TTGTGTGCTT GTGTTTTCTA CCACAGACAG
  541 GGAATCTTTT GAAGCGATTT CCAGCTGGAG AGAAAAAGTA GTAGCGGANG TTGGAGANAT
  601 ACCNACCGCA CTGGTGCNAA ACNAGATCGA TCTGCTGGAT GANTCNTGNA TCNAAATGAN
  661 GNANCGNANG GNNNNGNCNA AANNNTNANN CTGNNNNCNN NNNNNNNTNN NNGNNNNNNN
721 NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]