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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab11B (#RDB17258)

Expression vector of mouse Rab11B fused with EGFP.

Clone info. Expression vector of mouse Rab11B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab11b cDNA
Reference of insert sequence AB232606 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17258 pEGFP-C1-Rab11B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab11B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17258).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17258_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17258_A9Ela.seq check
>D06280E4_A9El_1_EGFP-C2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNCGN TCNCNTGGNC CTGCTGGANT TCGTGACCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGGGAC CCGGGACGAC
  121 GAGTACGATT ACCTATTCAA AGTGGTGCTT ATTGGGGACT CAGGTGTAGG TAAGAGCAAC
  181 CTGCTGTCAC GCTTCACCAG AAACGAATTC AACCTAGAGA GCAAGAGTAC CATCGGAGTG
  241 GAGTTCGCCA CTCGCAGCAT TCAGGTGGAC GGCAAGACCA TCAAGGCTCA GATCTGGGAC
  301 ACTGCTGGCC AGGAGCGCTA CCGTGCCATT ACCTCTGCGT ACTACCGTGG TGCAGTGGGT
  361 GCACTGCTGG TATATGACAT TGCCAAGCAC TTGACATATG AGAACGTGGA GCGCTGGCTG
  421 AAGGAGCTGC GGGATCATGC AGATAGCAAC ATTGTCATCA TGCTGGTGGG CAACAAGAGT
  481 GACCTGCGCC ACCTTCGGGC TGTGCCCACT GATGAGGCCC GTGCCTTTGC AGAAAAGAAC
  541 AACTTGTCCT TCATTGAGAC CTCAGCCTTG GATTCCACCA ATGTAGAGGA AGCATTCAAG
  601 AACATCCTCA CAGAAATCTA CCGTATTGTG TCACAGAAGC AAATCGCTGA NCGTGCAGCC
  661 CACGATGAGT CCCCTGGCAA CAACGTGGTG GACATCAGTG TGCCACCCAC CACCGATGGA
  721 CAGAGACCCA ACAAGCTGCA GTGCTGCCAG ANCCTGTGAA ATCACTAGTG AATTCGCGGN
  781 CGCCNGCNNG NCGACNNNAC CGCGGGCCCG GGNTNNACCG GNNCNAGATA ACNGATCATN
  841 ATCNGCCNTA NCANNTTTGT AGANGNTTTA CNTGCNTTNA AAAANNNCCN NNNNNNCCCC
  901 NNNNANNNGA ANNNTAAAAT GNATGNNNNN GNTGNNNNTN ACNNGNTTAN NNNNNCTTAN
  961 NANGGNNANN ANNNANNNNN NANNNTNNNN ANNTNNNNNA NNNANNNNNT TTTTNNNNNN
1021 NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17258_A9Elb.seq check
>D06280E4_A9El_1_SV40polyA-R2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNCNNCNNNN NNNNNGTTAA CNANNACNAT TGCATTCNTT TTATGTTTCN
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT
  181 GCAGGCGGCC GCGAATTCAC TAGTGATTTC ACAGGCTCTG GCAGCACTGC AGCTTGTTGG
  241 GTCTCTGTCC ATCGGTGGTG GGTGGCACAC TGATGTCCAC CACGTTGTTG CCAGGGGACT
  301 CATCGTGGGC TGCACGGTCA GCGATTTGCT TCTGTGACAC AATACGGTAG ATTTCTGTGA
  361 GGATGTTCTT GAATGCTTCC TCTACATTGG TGGAATCCAA GGCTGAGGTC TCAATGAAGG
  421 ACAAGTTGTT CTTTTCTGCA AAGGCACGGG CCTCATCAGT GGGCACAGCC CGAAGGTGGC
  481 GCAGGTCACT CTTGTTGCCC ACCAGCATGA TGACAATGTT GCTATCTGCA TGATCCCGCA
  541 GCTCCTTCAG CCAGCGCTCC ACGTTCTCAT ATGTCAAGTG CTTGGCAATG TCATATACCA
  601 GCAGTGCACC CACTGCACCA CGGTAGTACG CAGAGGTAAT GGCACGGTAG CGCTCCTGGC
  661 CAGCAGTGTC CCAGATCTGA GCCTTGATGG TCTTGCCGTC CACCTGAATG CTGCGAGTGG
  721 CGAACTCCAC TCCGATGGTA CTCTTGCTCT CTANGTTGAA TTCGTTTCTG GTGAAGCGTG
  781 NCAGCANGTT GCTCTTACCT ACACCTGANT CCCCAATAAG CACCACTTTG AATANGTAAT
  841 CGTACTCGTC GTCCCNGGTN CCCANNGATC TGANTNCGGA NTTGTNCAGC TCGTCCNTGN
  901 CNANANTGNT NCCNGNGGCN GNCNCNAACT CCANCNNNAN NATGTGNTCN CGCTTCTCGN
961 NNGGGNCTTT GCNCNNNNNN NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17258_A9Elc.seq check
>D06280E4_A9El_1_CMV-Forward_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC AANNGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC
  121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
  721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAAGCGN NNNNNCATGG
  781 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CNTGGACNAN CTGTACAAGT
  841 CCGGANTCAG ATCCATGGGG GANCCGGGAN NACGANTACN ATTACNNATT CNAAGTGGTG
  901 CTNNTTGGGG GANTCNNGTG TNNGNNAANA GCAACCTGCT GTCACGCNTT CNCNNNAANN
  961 NNATTCNNCN NNNGANNNCN NNANTANCNN TCGNANTGNN NTNCGCCANN CNCNGCATTN
 1021 NNNNTGGNAN NGNNNNNNAN CATCANNGNN NNNNATNNNN GNNNNNNGCN NGNNNNNNNN
1081 NN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17258_A9Eld.seq check
>D06280E4_A9El_1_SV40pro_F_V2_D03_12_ABI24.ab1
    1 NNNNNNNNTN NNNNNNNNNN NNNGGCTTTT GCNNNGNNCG ATCAAGAGNC AGGNTGAGGN
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CNGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGANTTCTT CTGANCGGGN NTCTGGGGTT CGAANTGANC GACCNANCNN
  901 NGCCCAACCT GNCNNCACGA GATTTCNANT NCNNCGCCNN CTTCNATNAA NNNNGGGCNT
961 NNNNNNCNTT TTNNNGGNNN CCNGNTGGAT GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]