Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab11A (#RDB17257)

Expression vector of mouse Rab11A fused with EGFP.

Clone info. Expression vector of mouse Rab11A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab11a cDNA
Reference of insert sequence AB232605 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17257 pEGFP-C1-Rab11A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab11A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17257).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17257_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert
Sequence file: RDB17257_A9Ela.seq check
>D06280E3_A9El_2_EGFP-C2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GGTCCTGCTG GANTTCGTGN NCGCCGCCGG
   61 GNTCNCTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGG GCACCCGCGA
  121 CGACGAGTAC GACTACCTCT TTAAAGTTGT CCTTATTGGA GATTCTGGTG TTGGAAAGAG
  181 TAACCTCCTG TCTCGATTTA CTCGAAATGA GTTTAATCTG GAAAGCAAGA GTACCATTGG
  241 AGTAGAGTTT GCAACAAGAA GCATCCAGGT TGATGGGAAA ACAATAAAGG CACAGATATG
  301 GGACACAGCA GGGCAGGAGC GGTACAGGGC TATAACGTCT GCATACTATC GTGGAGCAGT
  361 AGGTGCCTTA TTGGTTTATG ACATTGCTAA GCATCTCACA TATGAAAATG TAGAGCGATG
  421 GCTGAAAGAA CTGAGAGATC ATGCTGATAG TAACATTGTT ATCATGCTTG TGGGCAATAA
  481 GAGTGATTTA CGTCATCTCA GGGCAGTTCC TACAGATGAA GCAAGAGCTT TTGCAGAGAA
  541 GAATGGTTTG TCATTCATTG AGACATCTGC TCTAGATTCT ACAAATGTTG AAGCTGCTTT
  601 TCAGACAATT CTAACAGAGA TATACCGCAT TGTTTCTCAG AAGCAAATGT CAGACAGACG
  661 TGAAAATGAC ATGTCTCCAA GCAACAATGT GGTTCCTATT CATGTTCCGC CCACCACTGA
  721 GAACAAGCCA AAGGTGCAGT GCTGTCAGAA CATCTAAAAT CACTAGTGAA TTCTGCAGTC
  781 GACGGTACCG CGGNNNCNGG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC
  841 ACATTTGTNN AGGTTTTACT TGCTTTAAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
  901 NCATNAAANN GAATGCAATT GTNGTTGTNN ACTTGTTTAT TGCAGCTTAT AANGNNTANN
  961 ANNNAAGCAN TAGCNTCACN NNNNNNCACA ANTNAANNCA TTTTTTTNCA CTGCNNNCNN
1021 GNTNGNNGNN TTNNNNCNNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17257_A9Elb.seq check
>D06280E3_A9El_2_SV40polyA-R2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA AANAAGTTAA CNNNNNNNAT TGCATTCATT TTATGTTTCN
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
  181 CAGAATTCAC TAGTGATTTT AGATGTTCTG ACAGCACTGC ACCTTTGGCT TGTTCTCAGT
  241 GGTGGGCGGA ACATGAATAG GAACCACATT GTTGCTTGGA GACATGTCAT TTTCACGTCT
  301 GTCTGACATT TGCTTCTGAG AAACAATGCG GTATATCTCT GTTAGAATTG TCTGAAAAGC
  361 AGCTTCAACA TTTGTAGAAT CTAGAGCAGA TGTCTCAATG AATGACAAAC CATTCTTCTC
  421 TGCAAAAGCT CTTGCTTCAT CTGTAGGAAC TGCCCTGAGA TGACGTAAAT CACTCTTATT
  481 GCCCACAAGC ATGATAACAA TGTTACTATC AGCATGATCT CTCAGTTCTT TCAGCCATCG
  541 CTCTACATTT TCATATGTGA GATGCTTAGC AATGTCATAA ACCAATAAGG CACCTACTGC
  601 TCCACGATAG TATGCAGACG TTATAGCCCT GTACCGCTCC TGCCCTGCTG TGTCCCATAT
  661 CTGTGCCTTT ATTGTTTTCC CATCAACCTG GATGCTTCTT GTTGCAAACT CTACTCCAAT
  721 GGTACTCTTG CTTTCCAGAT TAAACTCATT TCGAGTAAAT CGAGACAGGA GGTTACTCTT
  781 TCCAACACNA GAATCTCCAA TAAGGACAAC TTTAAAGAGG TAGTCGTACT CGTCGTCGCG
  841 GGTGCCCATG GATCTGANTC CGGACTTGTA CAGCTCGTCC ATGCCGANAG TGATCCCNGC
  901 GGCGGTCACG AACTNCAGCN NGANCATGTG ATCNCGCTTC TCGTTGGGGT CTTTGCTCNN
  961 GGNNNANTGG GTGCTNNNGN ANNGGNTNGN CGGGCNNCNN CNNNGGNCNN NNGNNNNNGG
1021 GGGNGNNCNG CNNGNANNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17257_A9Elc.seq check
>D06280E3_A9El_2_CMV-Forward_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNNCNAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGANC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GANCGCCGCC GGGATCACTC TCGGCATGGA CGANCTGTAC AAGTCCGGAN
  841 TCAGATNCNT GGGCACCCGC GACGACNAGT ACNACTACCT CTTTAAAGTT GTNCTTATTG
  901 GAGANTCTGG NGTTGGAAAG AGTNACCNNC TGTCTCNNTT TACNCNAANT GANTTTANTC
  961 TGGAAAGCNN GANTANNNTN NNANTANANT TNNNNNNNAN NAANCATCCN NNNNGATGGN
1021 NAANNNNNNA ANNNNNNNNN N
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17257_A9Eld.seq check
>D06280E3_A9El_2_SV40pro_F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTTTTGC NNNNNNCGAT CNAGAGNNAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACN GTATCGCCGC TCCCGATTCG CANCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GANTTCTTCT GAGCGGGANT CNNGGGGNNN NAAATGANCN ANCAANCGAC
  901 GCCCAACCNN NNNTCNCNAG ATTTCGATTC CANCGNNGCC TTCTATGAAA NNNNNGGCTN
961 NNNANNCGTT TTNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]