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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab11A

Expression vector of mouse Rab11A fused with EGFP.

Catalog number RDB17257
Resource name pEGFP-C1-Rab11A
Clone info. Expression vector of mouse Rab11A fused with EGFP at N-terminus.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab11a cDNA
Reference of nucleotide sequence AB232605 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab11a (NCBI Gene 53869) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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Schedule A (GFP-A) [Link]
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RDB17257 pEGFP-C1-Rab11A DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17257_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP 3', insert
Sequence file: RDB17257_A9Ela.seq check
>D06280E3_A9El_2_EGFP-C2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT GGTCCTGCTG GANTTCGTGN NCGCCGCCGG
   61 GNTCNCTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGG GCACCCGCGA
  121 CGACGAGTAC GACTACCTCT TTAAAGTTGT CCTTATTGGA GATTCTGGTG TTGGAAAGAG
  181 TAACCTCCTG TCTCGATTTA CTCGAAATGA GTTTAATCTG GAAAGCAAGA GTACCATTGG
  241 AGTAGAGTTT GCAACAAGAA GCATCCAGGT TGATGGGAAA ACAATAAAGG CACAGATATG
  301 GGACACAGCA GGGCAGGAGC GGTACAGGGC TATAACGTCT GCATACTATC GTGGAGCAGT
  361 AGGTGCCTTA TTGGTTTATG ACATTGCTAA GCATCTCACA TATGAAAATG TAGAGCGATG
  421 GCTGAAAGAA CTGAGAGATC ATGCTGATAG TAACATTGTT ATCATGCTTG TGGGCAATAA
  481 GAGTGATTTA CGTCATCTCA GGGCAGTTCC TACAGATGAA GCAAGAGCTT TTGCAGAGAA
  541 GAATGGTTTG TCATTCATTG AGACATCTGC TCTAGATTCT ACAAATGTTG AAGCTGCTTT
  601 TCAGACAATT CTAACAGAGA TATACCGCAT TGTTTCTCAG AAGCAAATGT CAGACAGACG
  661 TGAAAATGAC ATGTCTCCAA GCAACAATGT GGTTCCTATT CATGTTCCGC CCACCACTGA
  721 GAACAAGCCA AAGGTGCAGT GCTGTCAGAA CATCTAAAAT CACTAGTGAA TTCTGCAGTC
  781 GACGGTACCG CGGNNNCNGG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC
  841 ACATTTGTNN AGGTTTTACT TGCTTTAAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
  901 NCATNAAANN GAATGCAATT GTNGTTGTNN ACTTGTTTAT TGCAGCTTAT AANGNNTANN
  961 ANNNAAGCAN TAGCNTCACN NNNNNNCACA ANTNAANNCA TTTTTTTNCA CTGCNNNCNN
 1021 GNTNGNNGNN TTNNNNCNNN NNNNNNNNNG TANNNTNANN GCNNAANNNG NNANNCNNNN
 1081 NNNNTTNNNN NANNNNNNNN NNNNNANTTT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNCNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNN
//
Primer: SV40polyA_R2
Region: insert 3'
Sequence file: RDB17257_A9Elb.seq check
>D06280E3_A9El_2_SV40polyA-R2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA AANAAGTTAA CNNNNNNNAT TGCATTCATT TTATGTTTCN
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
  181 CAGAATTCAC TAGTGATTTT AGATGTTCTG ACAGCACTGC ACCTTTGGCT TGTTCTCAGT
  241 GGTGGGCGGA ACATGAATAG GAACCACATT GTTGCTTGGA GACATGTCAT TTTCACGTCT
  301 GTCTGACATT TGCTTCTGAG AAACAATGCG GTATATCTCT GTTAGAATTG TCTGAAAAGC
  361 AGCTTCAACA TTTGTAGAAT CTAGAGCAGA TGTCTCAATG AATGACAAAC CATTCTTCTC
  421 TGCAAAAGCT CTTGCTTCAT CTGTAGGAAC TGCCCTGAGA TGACGTAAAT CACTCTTATT
  481 GCCCACAAGC ATGATAACAA TGTTACTATC AGCATGATCT CTCAGTTCTT TCAGCCATCG
  541 CTCTACATTT TCATATGTGA GATGCTTAGC AATGTCATAA ACCAATAAGG CACCTACTGC
  601 TCCACGATAG TATGCAGACG TTATAGCCCT GTACCGCTCC TGCCCTGCTG TGTCCCATAT
  661 CTGTGCCTTT ATTGTTTTCC CATCAACCTG GATGCTTCTT GTTGCAAACT CTACTCCAAT
  721 GGTACTCTTG CTTTCCAGAT TAAACTCATT TCGAGTAAAT CGAGACAGGA GGTTACTCTT
  781 TCCAACACNA GAATCTCCAA TAAGGACAAC TTTAAAGAGG TAGTCGTACT CGTCGTCGCG
  841 GGTGCCCATG GATCTGANTC CGGACTTGTA CAGCTCGTCC ATGCCGANAG TGATCCCNGC
  901 GGCGGTCACG AACTNCAGCN NGANCATGTG ATCNCGCTTC TCGTTGGGGT CTTTGCTCNN
  961 GGNNNANTGG GTGCTNNNGN ANNGGNTNGN CGGGCNNCNN CNNNGGNCNN NNGNNNNNGG
 1021 GGGNGNNCNG CNNGNANNNN NNNNNNANCT GNNNNCNNNC NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNTNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNN
//
Primer: CMV_Forward
Region: CMV pro, EGFP 5'
Sequence file: RDB17257_A9Elc.seq check
>D06280E3_A9El_2_CMV-Forward_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNNCNAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGANC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GANCGCCGCC GGGATCACTC TCGGCATGGA CGANCTGTAC AAGTCCGGAN
  841 TCAGATNCNT GGGCACCCGC GACGACNAGT ACNACTACCT CTTTAAAGTT GTNCTTATTG
  901 GAGANTCTGG NGTTGGAAAG AGTNACCNNC TGTCTCNNTT TACNCNAANT GANTTTANTC
  961 TGGAAAGCNN GANTANNNTN NNANTANANT TNNNNNNNAN NAANCATCCN NNNNGATGGN
 1021 NAANNNNNNA ANNNNNNNNN NNNGGNNNNN NNNNNNGNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNN
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB17257_A9Eld.seq check
>D06280E3_A9El_2_SV40pro_F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTTTTGC NNNNNNCGAT CNAGAGNNAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACN GTATCGCCGC TCCCGATTCG CANCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GANTTCTTCT GAGCGGGANT CNNGGGGNNN NAAATGANCN ANCAANCGAC
  901 GCCCAACCNN NNNTCNCNAG ATTTCGATTC CANCGNNGCC TTCTATGAAA NNNNNGGCTN
  961 NNNANNCGTT TTNNNGNNNN NNNNNNNNNN NGATNNNNNN NCNNNGGNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN GGNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNNNNNNGGN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.01.09

GNP_filter3_RDBDEP_html_191130.pl