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pEGFP-C1-Rab9B

Expression vector of mouse Rab9B fused with EGFP.

Catalog number RDB17256
Resource name pEGFP-C1-Rab9B
Clone info. Expression vector of mouse Rab9B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab9b cDNA
Reference of insert sequence AB232603 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab9b

          Reference sequence
            
           
          Sequence (full) RDB17256zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17256 pEGFP-C1-Rab9B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17256_A9Elp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3',insert
          Sequence file: RDB17256_A9Ela.seq check
          >D06280E2_A9El_2_EGFP-C2_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGAGTT CGTGANCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGAGTGGG AAATCCCTTC
            121 TCTTAAAGGT CATCCTCTTG GGTGATGGAG GAGTTGGGAA AAGCTCACTT ATGAACCGTT
            181 ATGTAACCAA CAAGTTCGAC TCCCAGGCTT TTCACACGAT AGGGGTAGAG TTCTTAAATC
            241 GAGATCTGGA GGTAGATGGA CGCTTTGTGA CCCTCCAGAT CTGGGACACT GCGGGGCAGG
            301 AACGTTTCAA GAGCCTTAGA ACACCATTCT ACAGGGGAGC CGACTGCTGC CTGCTAACCT
            361 TCAGTGTGGA TGACCGGCAG AGCTTTGAGA ACCTTGGTAA CTGGCAAAAA GAGTTCATCT
            421 ACTATGCAGA TGTAAAGGAC CCAGATCATT TCCCCTTTGT GGTTCTGGGT AACAAGGTAG
            481 ACAAAGAGGA TAGGCAAGTA ACGACTGAGG AGGCACAAGC CTGGTGCATG GAGAATGGAA
            541 ATTACCCTTA TCTAGAAACA AGTGCCAAAG ATGATACTAA TGTGACAGTG GCCTTTGAAG
            601 AAGCTGTTAG GCAGGTGTTA GCTGTAGAGG AACAGCTGGA ACATTGTATG TTAGGTCACA
            661 CCATTGATTT GAACAGTGGC TCCAAAGCAA GTTCTTCTTG TTGTTAGAGG TAGGGACTCA
            721 ATCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGATCTA GATAACTGAT
            781 CATAATCAGC CATACCACAT TTGTAGANGT TTTACTTGCT TTAAAAAACC TCCCACACCN
            841 CCCCCNNAAC CTGAAACNTN AAATGAATGC NATTGTTGTT GTTAACTTGT TTATTGNAGC
            901 TTATNNNGGT TACNAATAAA GCNNNAGCAT CNCNAATTTC NCAAATAAAG CATTTTTTTC
            961 NNNGCATTCT NNTTGTGGNT TGNNNNAACT CNTNNATGNN TNNNNACNCN NNANNNNNNN
          1021 NNN
          //
          Primer: SV40polyA_R2
          Region: insert
          Sequence file: RDB17256_A9Elb.seq check
          >D06280E2_A9El_2_SV40polyA-R2_H07_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA ANNNNGTTAA CAACNACNAT TGCATTCNTT TTATGTTTCN
             61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
            121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
            181 CAGAATTCGA TTGAGTCCCT ACCTCTAACA ACAAGAAGAA CTTGCTTTGG AGCCACTGTT
            241 CAAATCAATG GTGTGACCTA ACATACAATG TTCCAGCTGT TCCTCTACAG CTAACACCTG
            301 CCTAACAGCT TCTTCAAAGG CCACTGTCAC ATTAGTATCA TCTTTGGCAC TTGTTTCTAG
            361 ATAAGGGTAA TTTCCATTCT CCATGCACCA GGCTTGTGCC TCCTCAGTCG TTACTTGCCT
            421 ATCCTCTTTG TCTACCTTGT TACCCAGAAC CACAAAGGGG AAATGATCTG GGTCCTTTAC
            481 ATCTGCATAG TAGATGAACT CTTTTTGCCA GTTACCAAGG TTCTCAAAGC TCTGCCGGTC
            541 ATCCACACTG AAGGTTAGCA GGCAGCAGTC GGCTCCCCTG TAGAATGGTG TTCTAAGGCT
            601 CTTGAAACGT TCCTGCCCCG CAGTGTCCCA GATCTGGAGG GTCACAAAGC GTCCATCTAC
            661 CTCCAGATCT CGATTTAAGA ACTCTACCCC TATCGTGTGA AAAGCCTGGG AGTCGAACTT
            721 GTTGGTTACA TAACGGTTCA TAAGTGAGCT TTTCCCNACT CCTCCATCAC CCAAGAGGAT
            781 GANCTTTAAG AGAANGGANT TCCCACTCAT GGNTCTGANT CCGGACTTGT ACAGCTCGTC
            841 CATGCCGAGA GTGATNCCGG CGGCNGTCAC NAACTNNNNC NNNNCNTGTG NNCGCGCNTC
            901 TCGTTGGGGT CTTTGCTCNN GNCGGANTGG GTGNTCNGNA GTGNNTNGTC GGGCANCANC
            961 NNNGGGCCGT CNNCNANGGG GGNGNNCNNN TNGNANNGGN NNGNNNANCT NCANNNNNCN
          1021 NNCNNNNNNG NNNNGNNNNN NNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro,EGFP 5'
          Sequence file: RDB17256_A9Elc.seq check
          >D06280E2_A9El_2_CMV-Forward_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNCNNNTTG NNNNNNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
            121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
            181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
            241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
            301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
            361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
            421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
            481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
            541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
            601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
            661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
            721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
            781 GGAGTTCGTG ANCGCCGCCG GGNTCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGANT
            841 CAGATCCATG AGTGNNAAAT CCCTTCTCTT AAANGNCATC NNCTTGGGTG ANGGANGANT
            901 TGGGAAAAGC TCANTTATGA ANCGNNATGT NACCNACAAG TTCNANNCCN ANGCTTTTCN
            961 NNCNATAGGG GTAGANTTNN TAANTCGANN NNNGGANNNA NANGGNNNNT TNNNNNNCNN
          1021 NNNNNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR_KanR
          Sequence file: RDB17256_A9Eld.seq check
          >D06280E2_A9El_2_SV40pro_F_V2_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGCTTTTGCA AGNNCGATCN NGAGACAGGN TGAGGANCGT
             61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
            601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGANC
            781 GCTTCCTCNT GCTTTACNGT ATCGCCGCTC CCGATTCNCA GCGCATCGCC TTCTATCGCC
            841 TTCTTGACNA NTTNNNNNNA NCGGGANTCT GGGGTTCGAA ATGACCGANC NAGCGACGCC
            901 NAACCTGCCN TCACGAGATT TCNATNNCNN NGCCGCCTTC TATGAAANGN NGGGCTNNNG
          961 NANNGTTTTN CNGNNNNNNN NNN
          //
          Primer: mVenus-M_F
          Region: EGFP 3',insert 5'
          Sequence file: RDB17256_A9Ele.seq check
          >17256_172546_B1JdF_mVenus-M_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNAN NNNCCNCNAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
             61 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
            121 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
            181 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA
            241 CTCAGATCCA TGAGTGGGAA ATCCCTTCTC TTAAAGGTCA TCCTCTTGGG TGATGGAGGA
            301 GTTGGGAAAA GCTCACTTAT GAACCGTTAT GTAACCAACA AGTTCGACTC CCAGGCTTTT
            361 CACACGATAG GGGTAGAGTT CTTAAATCGA GATCTGGAGG TAGATGGACG CTTTGTGACC
            421 CTCCAGATCT GGGACACTGC GGGGCAGGAA CGTTTCAAGA GCCTTAGAAC ACCATTCTAC
            481 AGGGGAGCCG ACTGCTGCCT GCTAACCTTC AGTGTGGATG ACCGGCAGAG CTTTGAGAAC
            541 CTTGGTAACT GGCAAAAAGA GTTCATCTAC TATGCAGATG TAAAGGACCC AGATCATTTC
            601 CCCTTTGTGG TTCTGGGTAA CAAGGTAGAC NAAGAGGANN NGCNNGTAAC GACNGNNGAN
            661 GCANNNGCCN NNNGCANNGN NANNNNNNTA CCCNTANCNN NAANCNAGNG NNNANGNNNN
          721 NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2022.08.12

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