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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab9B (#RDB17256)

Expression vector of mouse Rab9B fused with EGFP.

Clone info. Expression vector of mouse Rab9B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab9b cDNA
Reference of insert sequence AB232603 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB17256zzt01.seq compiled by us

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. (2006) Genes Cells 11, 1023-1037); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17256 pEGFP-C1-Rab9B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab9B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17256).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17256_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert
Sequence file: RDB17256_A9Ela.seq check
>D06280E2_A9El_2_EGFP-C2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGAGTT CGTGANCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGAGTGGG AAATCCCTTC
  121 TCTTAAAGGT CATCCTCTTG GGTGATGGAG GAGTTGGGAA AAGCTCACTT ATGAACCGTT
  181 ATGTAACCAA CAAGTTCGAC TCCCAGGCTT TTCACACGAT AGGGGTAGAG TTCTTAAATC
  241 GAGATCTGGA GGTAGATGGA CGCTTTGTGA CCCTCCAGAT CTGGGACACT GCGGGGCAGG
  301 AACGTTTCAA GAGCCTTAGA ACACCATTCT ACAGGGGAGC CGACTGCTGC CTGCTAACCT
  361 TCAGTGTGGA TGACCGGCAG AGCTTTGAGA ACCTTGGTAA CTGGCAAAAA GAGTTCATCT
  421 ACTATGCAGA TGTAAAGGAC CCAGATCATT TCCCCTTTGT GGTTCTGGGT AACAAGGTAG
  481 ACAAAGAGGA TAGGCAAGTA ACGACTGAGG AGGCACAAGC CTGGTGCATG GAGAATGGAA
  541 ATTACCCTTA TCTAGAAACA AGTGCCAAAG ATGATACTAA TGTGACAGTG GCCTTTGAAG
  601 AAGCTGTTAG GCAGGTGTTA GCTGTAGAGG AACAGCTGGA ACATTGTATG TTAGGTCACA
  661 CCATTGATTT GAACAGTGGC TCCAAAGCAA GTTCTTCTTG TTGTTAGAGG TAGGGACTCA
  721 ATCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC ACCGGATCTA GATAACTGAT
  781 CATAATCAGC CATACCACAT TTGTAGANGT TTTACTTGCT TTAAAAAACC TCCCACACCN
  841 CCCCCNNAAC CTGAAACNTN AAATGAATGC NATTGTTGTT GTTAACTTGT TTATTGNAGC
  901 TTATNNNGGT TACNAATAAA GCNNNAGCAT CNCNAATTTC NCAAATAAAG CATTTTTTTC
  961 NNNGCATTCT NNTTGTGGNT TGNNNNAACT CNTNNATGNN TNNNNACNCN NNANNNNNNN
1021 NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert
Sequence file: RDB17256_A9Elb.seq check
>D06280E2_A9El_2_SV40polyA-R2_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA ANNNNGTTAA CAACNACNAT TGCATTCNTT TTATGTTTCN
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
  181 CAGAATTCGA TTGAGTCCCT ACCTCTAACA ACAAGAAGAA CTTGCTTTGG AGCCACTGTT
  241 CAAATCAATG GTGTGACCTA ACATACAATG TTCCAGCTGT TCCTCTACAG CTAACACCTG
  301 CCTAACAGCT TCTTCAAAGG CCACTGTCAC ATTAGTATCA TCTTTGGCAC TTGTTTCTAG
  361 ATAAGGGTAA TTTCCATTCT CCATGCACCA GGCTTGTGCC TCCTCAGTCG TTACTTGCCT
  421 ATCCTCTTTG TCTACCTTGT TACCCAGAAC CACAAAGGGG AAATGATCTG GGTCCTTTAC
  481 ATCTGCATAG TAGATGAACT CTTTTTGCCA GTTACCAAGG TTCTCAAAGC TCTGCCGGTC
  541 ATCCACACTG AAGGTTAGCA GGCAGCAGTC GGCTCCCCTG TAGAATGGTG TTCTAAGGCT
  601 CTTGAAACGT TCCTGCCCCG CAGTGTCCCA GATCTGGAGG GTCACAAAGC GTCCATCTAC
  661 CTCCAGATCT CGATTTAAGA ACTCTACCCC TATCGTGTGA AAAGCCTGGG AGTCGAACTT
  721 GTTGGTTACA TAACGGTTCA TAAGTGAGCT TTTCCCNACT CCTCCATCAC CCAAGAGGAT
  781 GANCTTTAAG AGAANGGANT TCCCACTCAT GGNTCTGANT CCGGACTTGT ACAGCTCGTC
  841 CATGCCGAGA GTGATNCCGG CGGCNGTCAC NAACTNNNNC NNNNCNTGTG NNCGCGCNTC
  901 TCGTTGGGGT CTTTGCTCNN GNCGGANTGG GTGNTCNGNA GTGNNTNGTC GGGCANCANC
  961 NNNGGGCCGT CNNCNANGGG GGNGNNCNNN TNGNANNGGN NNGNNNANCT NCANNNNNCN
1021 NNCNNNNNNG NNNNGNNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17256_A9Elc.seq check
>D06280E2_A9El_2_CMV-Forward_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNTTG NNNNNNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  781 GGAGTTCGTG ANCGCCGCCG GGNTCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGANT
  841 CAGATCCATG AGTGNNAAAT CCCTTCTCTT AAANGNCATC NNCTTGGGTG ANGGANGANT
  901 TGGGAAAAGC TCANTTATGA ANCGNNATGT NACCNACAAG TTCNANNCCN ANGCTTTTCN
  961 NNCNATAGGG GTAGANTTNN TAANTCGANN NNNGGANNNA NANGGNNNNT TNNNNNNCNN
1021 NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17256_A9Eld.seq check
>D06280E2_A9El_2_SV40pro_F_V2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCTTTTGCA AGNNCGATCN NGAGACAGGN TGAGGANCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGANC
  781 GCTTCCTCNT GCTTTACNGT ATCGCCGCTC CCGATTCNCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACNA NTTNNNNNNA NCGGGANTCT GGGGTTCGAA ATGACCGANC NAGCGACGCC
  901 NAACCTGCCN TCACGAGATT TCNATNNCNN NGCCGCCTTC TATGAAANGN NGGGCTNNNG
961 NANNGTTTTN CNGNNNNNNN NNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'
Sequence file: RDB17256_A9Ele.seq check
>17256_172546_B1JdF_mVenus-M_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNNCCNCNAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
   61 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  121 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  181 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA
  241 CTCAGATCCA TGAGTGGGAA ATCCCTTCTC TTAAAGGTCA TCCTCTTGGG TGATGGAGGA
  301 GTTGGGAAAA GCTCACTTAT GAACCGTTAT GTAACCAACA AGTTCGACTC CCAGGCTTTT
  361 CACACGATAG GGGTAGAGTT CTTAAATCGA GATCTGGAGG TAGATGGACG CTTTGTGACC
  421 CTCCAGATCT GGGACACTGC GGGGCAGGAA CGTTTCAAGA GCCTTAGAAC ACCATTCTAC
  481 AGGGGAGCCG ACTGCTGCCT GCTAACCTTC AGTGTGGATG ACCGGCAGAG CTTTGAGAAC
  541 CTTGGTAACT GGCAAAAAGA GTTCATCTAC TATGCAGATG TAAAGGACCC AGATCATTTC
  601 CCCTTTGTGG TTCTGGGTAA CAAGGTAGAC NAAGAGGANN NGCNNGTAAC GACNGNNGAN
  661 GCANNNGCCN NNNGCANNGN NANNNNNNTA CCCNTANCNN NAANCNAGNG NNNANGNNNN
721 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]