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pEGFP-C1-Rab8B

Expression vector of mouse Rab8B fused with EGFP.

Catalog number RDB17254
Resource name pEGFP-C1-Rab8B
Clone info. Expression vector of mouse Rab8B fused with EGFP at N-terminus.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab8b cDNA
Reference of nucleotide sequence AB232601 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab8b (NCBI Gene 235442) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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RDB17254 pEGFP-C1-Rab8B DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17254_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP 3', insert
Sequence file: RDB17254_A9Ela.seq check
>D06280D9_A9El_2_EGFP-C2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGAA GACGTACGAT
  121 TATCTGTTCA AGCTGCTGCT CATCGGCGAC TCCGGCGTTG GCAAGACCTG CCTCCTGTTC
  181 CGCTTCTCAG AGGACGCCTT CAACACCACC TTTATCTCCA CCATCGGAAT TGATTTTAAA
  241 ATCAGAACGA TAGAACTCGA CGGAAAGAAA ATTAAACTTC AGATATGGGA CACGGCGGGC
  301 CAGGAAAGAT TCCGAACAAT TACGACAGCA TACTATAGAG GAGCCATGGG AATTATGCTG
  361 GTCTATGACA TCACCAATGA AAAATCCTTT GACAATATTA AAAATTGGAT AAGAAACATT
  421 GAAGAGCACG CCTCTTCAGA TGTTGAAAGA ATGATCCTGG GTAACAAATG TGATATGAAC
  481 GACAAAAGAC AAGTGTCGAA AGAGAGAGGG GAGAAGTTAG CAATTGACTA TGGGATTAAA
  541 TTTTTGGAGA CAAGTGCAAA GTCGAGTACA AATGTAGAAG AGGCATTTTT TACACTTGCA
  601 CGGGATATAA TGACAAAACT CAACAGGAAA ATGAACGACA GCAATTCATC AGGAGCAGGT
  661 GGGCCAGTGA AGATAACAGA GAGCCGGTCT AAGAAGACCA GTTTCTTCCG CTGTTCTCTG
  721 CTTTGAATCA CTAGTGAATT CTGCAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC
  781 TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
  841 CCTCCCACAC CTCCCCCNGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
  901 GTTTATTGCA NCTTATAANG GNTACAAATA AAGCAATAGC ATCACAAATT TCACNAATAA
  961 ANCNNTTTTT TTCACTGCAT TCNAGTTNNN GNTTTGNNCN ANCTCNNCNA NGTATCNTNN
 1021 ANNCNNNNNN NNNNNNNNNN NNNNTTNNNN NAANNNNNNN NTNNANNTTT TNNNNNNNNN
 1081 NNNNNNNNTT TNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNN
//
Primer: SV40polyA_R2
Region: insert 3'
Sequence file: RDB17254_A9Elb.seq check
>D06280D9_A9El_2_SV40polyA-R2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA ANNNGTTAAC NACNANNATT GCATTCNTTT TATGTTTCAG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCACT AGTGATTCAA AGCAGAGAAC AGCGGAAGAA ACTGGTCTTC TTAGACCGGC
  241 TCTCTGTTAT CTTCACTGGC CCACCTGCTC CTGATGAATT GCTGTCGTTC ATTTTCCTGT
  301 TGAGTTTTGT CATTATATCC CGTGCAAGTG TAAAAAATGC CTCTTCTACA TTTGTACTCG
  361 ACTTTGCACT TGTCTCCAAA AATTTAATCC CATAGTCAAT TGCTAACTTC TCCCCTCTCT
  421 CTTTCGACAC TTGTCTTTTG TCGTTCATAT CACATTTGTT ACCCAGGATC ATTCTTTCAA
  481 CATCTGAAGA GGCGTGCTCT TCAATGTTTC TTATCCAATT TTTAATATTG TCAAAGGATT
  541 TTTCATTGGT GATGTCATAG ACCAGCATAA TTCCCATGGC TCCTCTATAG TATGCTGTCG
  601 TAATTGTTCG GAATCTTTCC TGGCCCGCCG TGTCCCATAT CTGAAGTTTA ATTTTCTTTC
  661 CGTCGAGTTC TATCGTTCTG ATTTTAAAAT CAATTCCGAT GGTGGAGATA AAGGTGGTGT
  721 TGAAGGCGTC CTCTGAGAAG CGGAACAGGA NGCAGGTCTT GCCNACGCCG GAGTCGCCGA
  781 TGAGCAGCAN CTTGAACAGA TAATCGTACG TCTTCNCCAT GGATCTGANT NCGGACTTGT
  841 ACAGCTCGTC CATGCCNAGA GTGATNCCGG CNGNNGTCAN NANCTCCAGC NNNNCATGTG
  901 ATCGCNCTTC NCGTTGGGGT CTTTGCTCNN GNCGGANNGG GTGCTCNNGT ANTGGNTNGT
  961 NGGGNNNNNN CACNGGGNCN NNCGCNNANN GGGNGNNCTG CTNGNANNNG NNNNNNNANC
 1021 NNCNNNCTNN NNNNNNNGAN NNNNNNNNNN NNNNNNNANN NNNNCNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNN
//
Primer: CMV_Forward
Region: CMV pro, EGFP 5'
Sequence file: RDB17254_A9Elc.seq check
>D06280D9_A9El_2_CMV-Forward_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ANCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGCTGGANTT CGTGANCGCC GCCNGGNTCA CTCTCGGCAT GGACGANCTG TACAAGTCCN
  841 GANTCANATC CATGGCGAAN ANGTACGANT ATCTGTTCAA GCTGCTGCTC ATNGGCGANT
  901 CCGGCGTTGG NAAGANCNGC CNNCNGNNCC GCTTNNCNNA NNNNGCCTNC NANNCCACCT
  961 TTATCTCCAC CNTCNGGAAT TGATTTTNAA ANCNGNNNNA TANNANNTCN NNNNNAANAA
 1021 AANTNNANNN NNNNNNNNNG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN N
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB17254_A9Eld.seq check
>D06280D9_A9El_2_SV40pro_F_V2_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TNCNAAGNNC GATNNAGANN ACNGGNTGAG
   61 GNTCGTTTCN NNTGATTGAA CAAGNATGGA TTGCNCNCAG GTTCTCCGGC CGCTTGGGTG
  121 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
  181 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
  241 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
  301 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
  361 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
  421 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
  481 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
  541 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
  601 AGCATGCCCG ACGGCGAGGA TNTNNNCGTG ACCNNNNNCG ATGCCTGCTT GCCGAATATC
  661 ATGGTGGAAA ATNGGCCGCT TTTCTGGATT CATNNNNTGT GGCCGGCTGG GTGTGGCGGA
  721 NCNNCTATCA GGACATAGCG TTNGCTACCC GTGATANTGC TGAANANCTT GGCNGCGAAN
  781 TGGGCTGACC GCTTNCTCGN GCTTTACNGT ATCNNCGCTC CNNATTCNCN NNGCATCNCC
  841 TTCTATCNCC TTCTTGACGA NTTCTTNNGN NCNGGNNNTC NNGGGGNNCN AANNNNNCNG
  901 NNCAAGCNAN NNCCNANCNN NNNANCNCNN ANATTNNNAT TNNNNCGCCG CCTTCTANNA
  961 AANGGTNNGG GCNTNNNNNN NNNNNTTTNN NGNNNNNCCN GNNNNGNANN ANNCNNNNNN
 1021 CNNNGGGGNN NNNNNNGNNN GNNNNNNTNN NNCCNNNCNN NNNNNGGNNN NGNNNNNNNN
 1081 NNNNNNNNNN NGNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNNNNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402.
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829.

2020.01.09

GNP_filter3_RDBDEP_html_191130.pl