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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab7 (#RDB17253)

Expression vector of mouse Rab7 fused with EGFP.

Clone info. Expression vector of mouse Rab7 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

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RDB17253 pEGFP-C1-Rab7 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17253).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17253_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert
Sequence file: RDB17253_A9Ela.seq check
>D06280D8_A9El_1_EGFP-C2_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNCNCGNNC NCNTGGTCCT GCTGGAGTTC GTGNCCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGACCTCTA GGAAGAAAGT
  121 GTTGCTGAAG GTCATCATCC TGGGGGACTC TGGTGTTGGA AAGACCTCTC TCATGAACCA
  181 GTATGTGAAC AAGAAGTTCA GTAACCAGTA CAAAGCCACA ATAGGAGCGG ACTTTCTGAC
  241 CAAGGAGGTG ATGGTGGACG ACAGACTTGT TACCATGCAG ATCTGGGACA CAGCCGGTCA
  301 AGAACGGTTC CAGTCTCTTG GTGTGGCCTT CTACAGAGGT GCAGATTGCT GTGTTCTGGT
  361 GTTTGATGTG ACTGCCCCCA ACACTTTCAA AACCCTCGAC AGCTGGAGAG ACGAGTTTCT
  421 CATCCAGGCC AGCCCCCGGG ATCCCGAGAA CTTCCCTTTT GTTGTGTTGG GAAACAAGAT
  481 TGACCTGGAA AACAGACAAG TGGCCACAAA GAGGGCACAG GCTTGGTGCT ACAGCAAAAA
  541 CAACATTCCT TACTTCGAGA CCAGTGCCAA GGAGGCCATC AATGTGGAGC AGGCCTTCCA
  601 GACAATTGCT CGGAATGCCC TTAAACAGGA AACAGAAGTG GAACTGTACA ATGAATTCCC
  661 TGAACCCATC AAACTGGACA AGAATGACCG GGCCAAGGCC TCCGCAGAAA GCTGCAGTTG
  721 TTGAAATCAC TAGTGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCNGATCT
  781 AGATAACTGA TCATAATCAG CCNTACCACA TTTGTAGANG TTTTACTTGC TTTAAAAAAC
  841 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG NNATTGNTGT TGTTAACTTG
  901 TTTATTGCAN CTTATAATGG TTACNAATAN NNNNNTANCN TCNCAAATTT CACNANTAAA
  961 GCATTTTTTT CNCTGCANTC TANTTGTGGT TTGNNNNAAN NCNTNNNNGT NTCNTNNNCN
1021 CGTNANTNNN AANCNNNNNN NNTTNNNNNA ANNTNNNNNN NNANNTTTNN NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17253_A9Elb.seq check
>D06280D8_A9El_1_SV40polyA-R2_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNANNNN GTTANNNACA ACNATTGCAT TCNTTTTATG TTTCAGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTCAACAA CTGCAGCTTT CTGCGGAGGC CTTGGCCCGG TCATTCTTGT
  241 CCAGTTTGAT GGGTTCAGGG AATTCATTGT ACAGTTCCAC TTCTGTTTCC TGTTTAAGGG
  301 CATTCCGAGC AATTGTCTGG AAGGCCTGCT CCACATTGAT GGCCTCCTTG GCACTGGTCT
  361 CGAAGTAAGG AATGTTGTTT TTGCTGTAGC ACCAAGCCTG TGCCCTCTTT GTGGCCACTT
  421 GTCTGTTTTC CAGGTCAATC TTGTTTCCCA ACACAACAAA AGGGAAGTTC TCGGGATCCC
  481 GGGGGCTGGC CTGGATGAGA AACTCGTCTC TCCAGCTGTC GAGGGTTTTG AAAGTGTTGG
  541 GGGCAGTCAC ATCAAACACC AGAACACAGC AATCTGCACC TCTGTAGAAG GCCACACCAA
  601 GAGACTGGAA CCGTTCTTGA CCGGCTGTGT CCCAGATCTG CATGGTAACA AGTCTGTCGT
  661 CCACCATCAC CTCCTTGGTC AGAAAGTCCG CTCCTATTGT GGCTTTGTAC TGGTTACTGA
  721 ACTTCTTGTT CACATACTGG TTCATGAGAG ANGTCTTTCC NACACCAGAN TCCCCCANGA
  781 NGANGACCTT CAGCAACACT TTCTTCCTAG ANGTCATGGN TCTGANTCCG GANTTGTACA
  841 GCTCGTCCAT GCCGANAGTG ANNNNGCGGC GGTCACGAAN TNNNGCAGGA NCATGTGATN
  901 GCGCTTNNCG TTGGGGTCTT TGCTCNNGNC NGNNTGGNNG CTCNNNANNG NNNGNCNGGC
961 ANCANCNCGG GNNNNNCNNN NANGGGGGNG NNCTGCTNNN ANNGNNNNGN NNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17253_A9Elc.seq check
>D06280D8_A9El_1_CMV-Forward_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTGNNGCNNA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGANCACC CAGTCCGCCC TGAGCAAAGA CCCCNACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGANTTC GTGACCGCCG CCNGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCNGATCC ATGANCTCTA NGAAGAAAGT GTTGCTGAAN GGTCATCATC CTGGGGGANT
  901 CNGGNGNTGG AAANNNCNTC TCTCNTGAAC CAGTATGTGA ACNNNAANNT TCAGTNNNNN
961 GNNNNAANNC NNNNNNANGA NNNNNNNTTN NGANNNNNAN GTNNNGGNNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17253_A9Eld.seq check
>D06280D8_A9El_1_SV40pro_F_V2_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGCTTTTG CAAGNNCGAT NNAGAGNCNG GANGAGGNTC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG ANTGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CANCGCNTCG CCTTCTATCG
  841 NCTTCTTGAC NAGTTCTTCT GANCGGGNNT CTGGGGTNNN NNNTGACCGA CCAAGCGANG
  901 CCCNACCTGC NNTCNCGAGA TTTCNATTCC NNCGCCGCNN NCTNTGAANN GNTGGGCTTC
961 NNNNNNGTTT TNNNGGNNNN NNNNCNNGNN TGATNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]