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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab6D (#RDB17252)

Expression vector of human Rab6D (Rab41 in NCBI) fused with EGFP.

Clone info. Expression vector of human Rab6D [Rab41 in NCBI] fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human RAB41 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. & Fukuda, M. (2015) J. Biol. Chem. 290, 22250-22261)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. & Fukuda, M. (2015) J. Biol. Chem. 290, 22250-22261)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17252 pEGFP-C1-Rab6D DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab6D was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17252).

Reference section:

Aizawa, M., Fukuda, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17252_A9Elp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 ()
Sequence file: RDB17252_A9Ela.seq check
>D06280D7_A9El_2_EGFP-C2_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGTCTGCC TTTGGTCACG
  121 ACGAGGCCTG GATGGAGGCC GGAGGCTTTG GTCTGGAGGC TGCCGAAAGA ACGGAATACC
  181 AGTCTCTGTG CAAATCTAAA CTCTTATTCC TGGGAGAGCA GAGCGTAGGG AAGACATCCA
  241 TCATCAGCCG CTTCATGTAC AACAGCTTCG GCTGCGCCTG CCAGGCAACT GTTGGAATTG
  301 ACTTCTTGTC TAAGACCATG TACTTGGAGG ACCAAATAGT TCAGCTGCAG CTATGGGACA
  361 CAGCTGGCCA GGAGCGCTTT CACAGCCTAA TTCCTAGCTA CATTCGTGAT TCAACTATTG
  421 CAGTGGTTGT CTATGACATT ACAAACATCA ATTCTTTTAA GGAGACAGAT AAGTGGGTAG
  481 AACACGTGCG AGCAGAAAGA GGTGACGATG TTGTCATCAT GTTGTTGGGT AACAAGATTG
  541 ATTTGGATAA CAAAAGACAA GTCACTGCAG AACAGGGTGA AGAAAAATCC AGAAACCTCA
  601 ATGTGATGTT TATTGAGACC AGTGCCAAAA CCGGTTACAA CGTGAAAAAG CTGTTCCGGC
  661 GTGTGGCTTC TGCCCTTCTT TCCACAAGGA CTTCACCTCC ACCAAAAGAG GGGACGGTTG
  721 AAATCGAACT GGAATCCTTC GAGGAGTCAN GCAACAGAAG CTATTGTTGA AATCNCTAGT
  781 GAATTCNCGG NCGCCTGCNN GNCGACNGTA NCGCGGGCCC NGGATNNNNN NGATCNAGAT
  841 AACTGATCAT AATCNGCNNN ACCACNTTTG TANAGGTTTN NNTNGCTTTA AAAAANNTCC
  901 CNCNCCTCCC CNNNNNNNTG AANNNTNAAA TGNNNGNNNN NNNNGNNGNN NNNNNNNTNN
961 NNNNNNCNNN NNNNNNN
//
Primer: SV40polyA_R2 ()
Sequence file: RDB17252_A9Elb.seq check
>D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
  181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
  241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
  301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
  361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
  421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
  481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
  541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
  601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
  661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
  721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
  781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
  841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
  901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
  961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
1021 NNNNCNNNGG NNNNNNNNNN
//
Primer: CMV_Forward ()
Sequence file: RDB17252_A9Elc.seq check
>D06280D7_A9El_2_CMV-Forward_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCNN NTTGNNNCNA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGNNNNNNTT CGTGACCGCC GCCGGGNTCA CTCTCGGCNT GGACNANCTG TACAAGTCCG
  841 GACTCAGATC CATGTCTGCC TTTNGTCACN NCGANGCCTG NATGGANGNC GGAGGCTTTN
  901 GTCTGGANGC TGCCGAAANA NNNGNANTAC CNGTCTCTGN GNNAATCNNN NTCNNATTNC
  961 TGGGANANCN GANCNNNNNG GGAAGANNTC CATCNTCNNN CCNNNNTNNN NNNNNNANAN
1021 NNTNNNNNNN NNCNNNNCNN NGNNANNNNN NGNNNNNNNN NTNNNNNNNN NN
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB17252_A9Eld.seq check
>D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
  181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
  241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
  301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
  361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
  421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
  481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
  541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
  601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
  661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
  721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
  781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
  841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
  901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
  961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
1021 NNNNCNNNGG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]