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pEGFP-C1-Rab6D

Expression vector of human Rab6D (Rab41 in NCBI) fused with EGFP.

Catalog number RDB17252
Resource name pEGFP-C1-Rab6D
Clone info. Expression vector of human Rab6D [Rab41 in NCBI] fused with EGFP at N-terminus.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human RAB41 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank human RAB41 (NCBI Gene 347517) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. & Fukuda, M. (2015) J. Biol. Chem. 290, 22250-22261)
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Schedule A (GFP-A) [Link]
Remarks
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RDB17252 pEGFP-C1-Rab6D DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17252_A9Elp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 RDB17252_A9Ela.seq
>D06280D7_A9El_2_EGFP-C2_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGTCTGCC TTTGGTCACG
  121 ACGAGGCCTG GATGGAGGCC GGAGGCTTTG GTCTGGAGGC TGCCGAAAGA ACGGAATACC
  181 AGTCTCTGTG CAAATCTAAA CTCTTATTCC TGGGAGAGCA GAGCGTAGGG AAGACATCCA
  241 TCATCAGCCG CTTCATGTAC AACAGCTTCG GCTGCGCCTG CCAGGCAACT GTTGGAATTG
  301 ACTTCTTGTC TAAGACCATG TACTTGGAGG ACCAAATAGT TCAGCTGCAG CTATGGGACA
  361 CAGCTGGCCA GGAGCGCTTT CACAGCCTAA TTCCTAGCTA CATTCGTGAT TCAACTATTG
  421 CAGTGGTTGT CTATGACATT ACAAACATCA ATTCTTTTAA GGAGACAGAT AAGTGGGTAG
  481 AACACGTGCG AGCAGAAAGA GGTGACGATG TTGTCATCAT GTTGTTGGGT AACAAGATTG
  541 ATTTGGATAA CAAAAGACAA GTCACTGCAG AACAGGGTGA AGAAAAATCC AGAAACCTCA
  601 ATGTGATGTT TATTGAGACC AGTGCCAAAA CCGGTTACAA CGTGAAAAAG CTGTTCCGGC
  661 GTGTGGCTTC TGCCCTTCTT TCCACAAGGA CTTCACCTCC ACCAAAAGAG GGGACGGTTG
  721 AAATCGAACT GGAATCCTTC GAGGAGTCAN GCAACAGAAG CTATTGTTGA AATCNCTAGT
  781 GAATTCNCGG NCGCCTGCNN GNCGACNGTA NCGCGGGCCC NGGATNNNNN NGATCNAGAT
  841 AACTGATCAT AATCNGCNNN ACCACNTTTG TANAGGTTTN NNTNGCTTTA AAAAANNTCC
  901 CNCNCCTCCC CNNNNNNNTG AANNNTNAAA TGNNNGNNNN NNNNGNNGNN NNNNNNNTNN
  961 NNNNNNCNNN NNNNNNNNNN NANNNNNNNN NNNCNNNNNN NNNTNNNNNN NNNNNNNNNN
 1021 NTNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTN NNNNNNNNNN NNNNNNNNNN N
//
Primer: SV40polyA_R2 RDB17252_A9Elb.seq
>D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
  181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
  241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
  301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
  361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
  421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
  481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
  541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
  601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
  661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
  721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
  781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
  841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
  901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
  961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
 1021 NNNNCNNNGG NNNNNNNNNN NNNGGNGNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN
//
Primer: CMV_Forward RDB17252_A9Elc.seq
>D06280D7_A9El_2_CMV-Forward_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCNN NTTGNNNCNA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGNNNNNNTT CGTGACCGCC GCCGGGNTCA CTCTCGGCNT GGACNANCTG TACAAGTCCG
  841 GACTCAGATC CATGTCTGCC TTTNGTCACN NCGANGCCTG NATGGANGNC GGAGGCTTTN
  901 GTCTGGANGC TGCCGAAANA NNNGNANTAC CNGTCTCTGN GNNAATCNNN NTCNNATTNC
  961 TGGGANANCN GANCNNNNNG GGAAGANNTC CATCNTCNNN CCNNNNTNNN NNNNNNANAN
 1021 NNTNNNNNNN NNCNNNNCNN NGNNANNNNN NGNNNNNNNN NTNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NN
//
Primer: SV40pro_F_V2 RDB17252_A9Eld.seq
>D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
  181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
  241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
  301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
  361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
  421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
  481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
  541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
  601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
  661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
  721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
  781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
  841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
  901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
  961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
 1021 NNNNCNNNGG NNNNNNNNNN NNNGGNGNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

2019.07.23

GNP_filter3_RDBDEP_html_190432.pl