Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab6A (#RDB17249)

Expression vector of mouse Rab6A fused with EGFP.

Clone info. Expression vector of mouse Rab6A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab6a cDNA
Reference of insert sequence AB232596 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17249 pEGFP-C1-Rab6A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab6A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17249).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17249_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB17249_A9Ela.seq check
>D06280D4_A9El_2_EGFP-C2_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCG NTNNCNTGGT CCTGCTGGAG TTCGTGNCCG CCGCCGGGNT
   61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGTCCG CGGGCGGAGA
  121 CTTCGGGAAT CCGCTGAGGA AATTCAAGCT GGTGTTCCTG GGAGAGCAGA GCGTTGGAAA
  181 GACGTCCTTG ATCACCCGAT TCATGTATGA CAGTTTTGAC AACACCTATC AGGCAACAAT
  241 TGGCATTGAC TTCTTATCAA AAACAATGTA CTTGGAGGAT AGAACCGTGC GATTGCAATT
  301 ATGGGACACA GCAGGTCAAG AGCGGTTCAG GAGCTTGATT CCTAGCTACA TTCGAGACTC
  361 CACTGTGGCA GTTGTTGTTT ATGATATCAC AAATGTTAAC TCATTCCAGC AAACTACAAA
  421 ATGGATTGAT GATGTCAGAA CAGAGAGAGG AAGTGATGTC ATCATCATGC TAGTAGGAAA
  481 TAAAACAGAT CTTGCCGATA AGAGGCAAGT GTCAATTGAG GAGGGAGAGA GGAAAGCCAA
  541 AGAGCTGAAT GTTATGTTTA TTGAAACCAG TGCAAAAGCA GGATACAATG TAAAGCAGCT
  601 TTTCCGGCGT GTTGCTGCAG CTTTACCTGG AATGGAAAGC ACACAGGACA GAAGCAGAGA
  661 AGACATGATT GACATAAAAC TGGAAAAGCC TCAGGAGCAA CCAGTCAATG AANGCGGCTG
  721 TTCCTGCTAA AATCACTAGT GAATTCTGCA GTCGACGGTA CCGCGGGCCC NGGATCCACC
  781 GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACNTTTG TAGANGNTTT ACTTGCTTTA
  841 AAAAACCTCC CACANCNTCC CCNNGANNCT GAAACNTAAA ATGAATGCAA TTGTTGTTGT
  901 NNACTTGTTT ATTGCAGCNT ANNNNNGGTT ANNAATAAAG CNNTANCATC NNAANTTTNN
961 CAAATAAAGC ATTTTTTTTN NNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17249_A9Elb.seq check
>D06280D4_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNGCNNNNN NNNNGTTAAC AACAACAATT GCATTCNTTT TATGTTTCAG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCACT AGTGATTTTA GCAGGAACAG CCGCCTTCAT TGACTGGTTG CTCCTGAGGC
  241 TTTTCCAGTT TTATGTCAAT CATGTCTTCT CTGCTTCTGT CCTGTGTGCT TTCCATTCCA
  301 GGTAAAGCTG CAGCAACACG CCGGAAAAGC TGCTTTACAT TGTATCCTGC TTTTGCACTG
  361 GTTTCAATAA ACATAACATT CAGCTCTTTG GCTTTCCTCT CTCCCTCCTC AATTGACACT
  421 TGCCTCTTAT CGGCAAGATC TGTTTTATTT CCTACTAGCA TGATGATGAC ATCACTTCCT
  481 CTCTCTGTTC TGACATCATC AATCCATTTT GTAGTTTGCT GGAATGAGTT AACATTTGTG
  541 ATATCATAAA CAACAACTGC CACAGTGGAG TCTCGAATGT AGCTAGGAAT CAAGCTCCTG
  601 AACCGCTCTT GACCTGCTGT GTCCCATAAT TGCAATCGCA CGGTTCTATC CTCCAAGTAC
  661 ATTGTTTTTG ATAAGAAGTC AATGCCAATT GTTGCCTGAT AGGTGTTGTC AAAACTGTCA
  721 TACATGAATC GGGTGATCAA GGACGTCTTT CCNACGCTCT GCTCTCCCAG GAACACCAGC
  781 TTGAATTTCC TCAGCGGATT CCCGAAGTCT CCGCCCGCGG ANATGGNTCT GANTNCGGAN
  841 TTGTACAGCT CGTCCATGCC NAGAGTGATN CCGGCGGCGG TCACGAACTC CANCAGGANC
  901 ATGTGNTNNC GCTTCTCGTT GGGGTCTTTG CNCNNGNCNG ANNNGGNGCT CNNNTANTGG
  961 NTGNCNGGCA NCANCNNNGG NNNNNCNNNN ANGGGGGNGN NCTGCTNNNA NNNGNNNGNN
1021 NANNNNNNNN NN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB17249_A9Elc.seq check
>D06280D4_A9El_2_CMV-Forward_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACNT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG ANCTGTACAA GTCCGGACTC
  841 AGATCCATGT CCGCNGGCGG AGANTTCGGG AATNCGCTGA GGAAANTCNN GCTGGGTGTT
  901 NCTGGGANAG CANANCGTTG GAAAGACGTC CNTNGNTNAC CNNNNTCATG TATGANANTT
961 TTGACNACNN NTANNNNNNN NNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17249_A9Eld.seq check
>D06280D4_A9El_2_SV40pro_F_V2_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGCTTTTG CNNNGNNCGA TCAAGAGANA GGNTGAGGNT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGANCGCT
  721 ATCANGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA NCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GNCTTCTATC
  841 GCCTTCTTGA CGANTTCTTC TGANCGGGAN TCTGGGGNNC GAANTGACCG ACCAANCGAC
  901 GCCCNACCTG NCNTCACGAN ANTTCGANNC CNCCGCCNCN NTCTATGAAA NGTTGGGCTT
961 NNGNANNNTT TTNNNNGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]