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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab3D (#RDB17243)

Expression vector of mouse Rab3D fused with EGFP.

Clone info. Expression vector of mouse Rab3D fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab3d cDNA
Reference of insert sequence AB232590 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17243 pEGFP-C1-Rab3D DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab3D was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17243).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17243_A9Elp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB17243_A9Ela.seq check
>D06280C7_A9El_2_EGFP-C2_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGA TCNCATGGTC CTGCTGGAGT TCGTGNNCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCATC CGCTAGTGAG
  121 CCCCCTGCCA GCCCAAGAGA CGCTGCTGAC CAGAACTTTG ACTACATGTT CAAACTGCTC
  181 TTGATCGGGA ACAGCAGCGT GGGCAAGACA TCCTTCCTGT TCCGCTATGC CGATGACTCC
  241 TTCACCCCTG CCTTCGTGAG CACAGTGGGC ATCGACTTCA AGGTCAAGAC GGTCTACCGA
  301 CATGACAAGA GGATCAAGCT GCAGATTTGG GACACAGCAG GTCAGGAGCG CTACCGGACA
  361 ATCACCACGG CCTACTATCG CGGAGCTATG GGTTTCCTGC TCATGTATGA CATCGCCAAC
  421 CAGGAGTCCT TCACCGCGGT GCAGGACTGG GCTACGCAGA TCAAAACCTA TTCCTGGGAC
  481 AATGCCCAGG TAATCCTCGT GGGGAACAAG TGTGACCTGG AAGACGAACG GGTCGTACCT
  541 GCTGAGGATG GCCGGAGGCT CGCCGATGAT CTTGGTTTTG AGTTCTTTGA GGCCAGCGCC
  601 AAGGAGAATA TCAATGTGAA GCAGGTGTTC GAGCGCCTGG TGGACATCAT CTGTGACAAG
  661 ATGAATGAAT CCCTGGAACC CAGCTCCAGC CCAGGCAGCA ATGGAAAAGG CCCAGCCCTT
  721 GGGGATACCC CACCCCCNCA GCCGAGCAGC TGCAGCTGTT ANAATCACTA GTGAATTCTG
  781 CAGTCGACGG TACCGCGGGC CCGGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC
  841 NTACCACATT TGTAGANGNT TTACTNGCTT TAAAAAANCT CCNNCACCNN CCCCNGAACC
  901 TGAAACATAA AANGANTGNN NNTGNTGNNG TTAACTTGTT TANNGCAGCT TANNANGGNT
  961 ACNANTAAAG CNNTANNNTC NNNNNTTNCN NNANTNANNN NTTTTTTTTC NCTGCNNNNN
1021 NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17243_A9Elb.seq check
>D06280C7_A9El_2_SV40polyA-R2_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NAANNACANN NNNTGCATTC NTTTTATGTT
   61 TCNGGTTCNG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
  121 TATGGCTGAT TATGATCAGT TATCTAGATC CGGTGGATCC CGGGCCCGCG GTACCGTCGA
  181 CTGCAGAATT CACTAGTGAT TCTAACAGCT GCAGCTGCTC GGCTGTGGGG GTGGGGTATC
  241 CCCAAGGGCT GGGCCTTTTC CATTGCTGCC TGGGCTGGAG CTGGGTTCCA GGGATTCATT
  301 CATCTTGTCA CAGATGATGT CCACCAGGCG CTCGAACACC TGCTTCACAT TGATATTCTC
  361 CTTGGCGCTG GCCTCAAAGA ACTCAAAACC AAGATCATCG GCGAGCCTCC GGCCATCCTC
  421 AGCAGGTACG ACCCGTTCGT CTTCCAGGTC ACACTTGTTC CCCACGAGGA TTACCTGGGC
  481 ATTGTCCCAG GAATAGGTTT TGATCTGCGT AGCCCAGTCC TGCACCGCGG TGAAGGACTC
  541 CTGGTTGGCG ATGTCATACA TGAGCAGGAA ACCCATAGCT CCGCGATAGT AGGCCGTGGT
  601 GATTGTCCGG TAGCGCTCCT GACCTGCTGT GTCCCAAATC TGCAGCTTGA TCCTCTTGTC
  661 ATGTCGGTAG ACCGTCTTGA CCTTGAAGTC GATGCCCACT GTGCTCACGA NNGCANGGGT
  721 GAAGGAGTCA TCGGCATANN NGAANNANGG AANNGATGTC TTGCCCNNNN NGCTGTTCCC
  781 GATCAAGAGC AGTTTNNNNN TGTANTCAAA GTTCTGGTCA GCAGCGTCTC TTGGGCTGNC
  841 ANGGGGCTCA NNANCNGGAT GCCATGGNTC TGAGTNCGGA CTTGTACAGC TCGTCCATGN
  901 NNNNNNNNGA TNCCGGCGGN NGNCACNNAN CTCNNNCANG NNCNANGTGA TCNCGCTTCT
  961 CNNTNGGGGN NTTTGCTNNN GNNNGNNTGN NNNNTNNNNN NNNGNNNGNC GGNNNNNNNN
1021 NN
//
Primer: CMV_Forward (Pr0016)
Region: EGFP 5'
Sequence file: RDB17243_A9Elc.seq check
>D06280C7_A9El_2_CMV-Forward_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN TTGNNGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACNAGTCCGG
  841 ANTCAGATCC NTGGCATCCG CTANTGAGCC CCCTGCCNGC CCNAGANANG CTGCTGANNA
  901 NAACTTTGAN TACNTGTTNC NAACTGCTCT TGATNNGGNA CNGCANCNNG GGNNNAGANA
  961 TCCTNCCNGT TNNGCTATGN CNGANGANTN CNNTNNNCCC NNGCCNNNNT GANCNNNNNG
1021 GNNNNNNNNN N
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR 5'
Sequence file: RDB17243_A9Eld.seq check
>D06280C7_A9El_2_SV40pro_F_V2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNTTTT GNAAGNNCGN NNNNNAGANA GGNNGAGGNN
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCANGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CNTGCTTTAC GGTATCNNCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CNANTTCTTC TGANCGGGAN TCTNGGGTTC GAAATGANNG ANNNANCGAC
  901 NNCCANCNTG CNNTCNNNAN ATTTCNATTC CACCGCCNCC NTCNATGAAA GGNNNGGCNT
961 NNNNANNCNT TTNNCNGGNN NNNNGCTNNA TNATNNNNNN NCGCNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]