Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab3A

Expression vector of mouse Rab3A fused with EGFP.

Catalog number RDB17240
Resource name pEGFP-C1-Rab3A
Clone info. Expression vector of mouse Rab3A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab3a cDNA
Reference of insert sequence AB232587 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab3a

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17240 pEGFP-C1-Rab3A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17240_A9Elp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3', insert 5'
          Sequence file: RDB17240_A9Ela.seq check
          >D06280C4_A9El_1_EGFP-C2_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNCNCGNN CNCNTGGTCC TGCTGGANTT CGTGNCCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCTTCC GCCACAGACT
            121 CTCGCTATGG GCAGAAGGAG TCCTCAGACC AGAACTTCGA CTATATGTTC AAGATCCTGA
            181 TCATTGGGAA CAGCAGCGTG GGCAAAACCT CGTTCCTCTT CCGCTACGCA GATGACTCCT
            241 TCACTCCAGC CTTTGTCAGC ACCGTTGGCA TAGACTTCAA GGTCAAAACC ATCTATCGCA
            301 ACGACAAGAG GATCAAGCTG CAGATCTGGG ACACAGCAGG GCAAGAGCGG TACCGCACCA
            361 TCACCACAGC CTATTACCGA GGCGCCATGG GCTTCATCCT AATGTATGAC ATCACCAATG
            421 AGGAGTCATT TAATGCAGTG CAGGACTGGT CCACTCAGAT CAAAACTTAC TCGTGGGACA
            481 ATGCCCAGGT GCTGCTGGTG GGAAACAAGT GTGACATGGA AGATGAGCGA GTGGTGTCCT
            541 CAGAGCGTGG CCGGCAGCTG GCTGACCACC TGGGCTTTGA GTTCTTTGAG GCCAGCGCCA
            601 AGGACAACAT TAATGTCAAG CAGACGTTTG AACGTCTGGT GGACGTGATC TGTGAGAAGA
            661 TGTCAGAGTC CCTGGATACT GCAGACCCTG CGGTCACCGG TGCCAAGCAG GGCCCGCAGC
            721 TCACCGACCA GCANGCGCCA CCTCATCANG ATTGTGCCTG CTGAAATCAC TAGTGAATTC
            781 TGCAGTCGAC NGTACCGCGG GCCCNGGANC CACCGGATCT AGATAACTGA TCATAATCAG
            841 CCATACCACA TTTGTAGAGG NTTTACTTGC TTTAAAAAAC CTCCCNCACC TCCCCCTGAA
            901 NCTGAAACNT AAAATGAATG CAATTGTTGN TGTTAACTTG NTTANNGCAN CTTATNNNGG
          961 NTACNAANAA AGCNNNANCN TCANNNNTTN NNNAATAANG CATTTTTTNN NNNNNNNN
          //
          Primer: SV40polyA_R2
          Region: insert 3'
          Sequence file: RDB17240_A9Elb.seq check
          >D06280C4_A9El_1_SV40polyA-R2_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNANNAANN NGNNNNNNNN NANNATTGCA TTCNTTTTAT GTTTCNGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
            181 ATTCACTAGT GATTTCAGCA GGCACAATCC TGATGAGGTG GCGCCTGCTG GTCGGTGAGC
            241 TGCGGGCCCT GCTTGGCACC GGTGACCGCA GGGTCTGCAG TATCCAGGGA CTCTGACATC
            301 TTCTCACAGA TCACGTCCAC CAGACGTTCA AACGTCTGCT TGACATTAAT GTTGTCCTTG
            361 GCGCTGGCCT CAAAGAACTC AAAGCCCAGG TGGTCAGCCA GCTGCCGGCC ACGCTCTGAG
            421 GACACCACTC GCTCATCTTC CATGTCACAC TTGTTTCCCA CCAGCAGCAC CTGGGCATTG
            481 TCCCACGAGT AAGTTTTGAT CTGAGTGGAC CAGTCCTGCA CTGCATTAAA TGACTCCTCA
            541 TTGGTGATGT CATACATTAG GATGAAGCCC ATGGCGCCTC GGTAATAGGC TGTGGTGATG
            601 GTGCGGTACC GCTCTTGCCC TGCTGTGTCC CAGATCTGCA GCTTGATCCT CTTGTCGTTG
            661 CGATAGATGG TTTTGACCTT GAAGTCTATG CNAACGGTGC TGACAAAGGC TGGAGTGAAG
            721 GANTCATCTG CGTAGCGGAA GANGAACGAN GTTTTGCCCA CGCTGCTGTT CNCNATGATC
            781 AGGATCTTGA ACATATAGTC GAANTTCTGG TCTGANGANT CCTTCTGCCN NTANCGAGAN
            841 TCTGTGGCGG AAGCNATGGN TCTGANTNCG GANTTGTNCA GCTCGTCCAN GCCGANANTG
            901 ATNCCNGNGG NNGNCACNAA NNNCNNCNNN ANCNTGTNAT NGCNCTTNNC NNTNGGGNNN
          961 NTNGCNCNNG NNNGNANNNG GNGNNCNNNN NNNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro, EGFP 5'
          Sequence file: RDB17240_A9Elc.seq check
          >D06280C4_A9El_1_CMV-Forward_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCCN TTGNNGCNAN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
            121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
            181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
            241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
            301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
            361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
            421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
            481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
            541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
            601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
            661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
            721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
            781 GCTGGANTTC GTGANCGCCG CCNGGATCAC TCTCGGCATG GACGANCTGT ACAAGTCCGG
            841 ANTCAGATCC ATGGCTTCCG CCACAGACTC TCGCTATGGG CAGAANGANT CCNCNNANCA
            901 GAACTTCNAC TATATGTTCN NNATNCNGAT CATNNGGAAC AGCANCGTGG GNAAAACCNC
            961 NTTCCNCTTN CNNNNNNCNN ANGANNCCNN CNNNNNNNCC NTTNNNANCN NNNNTNGNNN
          1021 NNNNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR_KanR
          Sequence file: RDB17240_A9Eld.seq check
          >D06280C4_A9El_1_SV40pro_F_V2_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGN NNAGNNCGAT CNNGAGACAG GATGAGGNTC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA
            781 NCGCTTCCTC GTGCTTTACN GNATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCNTGAC NANTTCTTCT GANCNGGANT CTGGGGTTCG AAATGANCNA NNNAGCGANG
            901 CCCNANCTGN CNTCACGAGA TTTCNATTNC ACCGCNNCNN TCTATNAANN NTGGNNNTCN
          961 GNANNCNNTT TNCNNGNNNN NCNNNCNNGN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl