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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab3A

Expression vector of mouse Rab3A fused with EGFP.

Catalog number RDB17240
Resource name pEGFP-C1-Rab3A
Clone info. Expression vector of mouse Rab3A fused with EGFP at N-terminus.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab3a cDNA
Reference of nucleotide sequence AB232587 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab3a (NCBI Gene 19339) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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Schedule A (GFP-A) [Link]
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RDB17240 pEGFP-C1-Rab3A DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17240_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Region: EGFP 3', insert 5'
Sequence file: RDB17240_A9Ela.seq check
>D06280C4_A9El_1_EGFP-C2_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCGNN CNCNTGGTCC TGCTGGANTT CGTGNCCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCTTCC GCCACAGACT
  121 CTCGCTATGG GCAGAAGGAG TCCTCAGACC AGAACTTCGA CTATATGTTC AAGATCCTGA
  181 TCATTGGGAA CAGCAGCGTG GGCAAAACCT CGTTCCTCTT CCGCTACGCA GATGACTCCT
  241 TCACTCCAGC CTTTGTCAGC ACCGTTGGCA TAGACTTCAA GGTCAAAACC ATCTATCGCA
  301 ACGACAAGAG GATCAAGCTG CAGATCTGGG ACACAGCAGG GCAAGAGCGG TACCGCACCA
  361 TCACCACAGC CTATTACCGA GGCGCCATGG GCTTCATCCT AATGTATGAC ATCACCAATG
  421 AGGAGTCATT TAATGCAGTG CAGGACTGGT CCACTCAGAT CAAAACTTAC TCGTGGGACA
  481 ATGCCCAGGT GCTGCTGGTG GGAAACAAGT GTGACATGGA AGATGAGCGA GTGGTGTCCT
  541 CAGAGCGTGG CCGGCAGCTG GCTGACCACC TGGGCTTTGA GTTCTTTGAG GCCAGCGCCA
  601 AGGACAACAT TAATGTCAAG CAGACGTTTG AACGTCTGGT GGACGTGATC TGTGAGAAGA
  661 TGTCAGAGTC CCTGGATACT GCAGACCCTG CGGTCACCGG TGCCAAGCAG GGCCCGCAGC
  721 TCACCGACCA GCANGCGCCA CCTCATCANG ATTGTGCCTG CTGAAATCAC TAGTGAATTC
  781 TGCAGTCGAC NGTACCGCGG GCCCNGGANC CACCGGATCT AGATAACTGA TCATAATCAG
  841 CCATACCACA TTTGTAGAGG NTTTACTTGC TTTAAAAAAC CTCCCNCACC TCCCCCTGAA
  901 NCTGAAACNT AAAATGAATG CAATTGTTGN TGTTAACTTG NTTANNGCAN CTTATNNNGG
  961 NTACNAANAA AGCNNNANCN TCANNNNTTN NNNAATAANG CATTTTTTNN NNNNNNNNNN
 1021 NNNNNNNNNT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 N
//
Primer: SV40polyA_R2
Region: insert 3'
Sequence file: RDB17240_A9Elb.seq check
>D06280C4_A9El_1_SV40polyA-R2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNANNAANN NGNNNNNNNN NANNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCAGCA GGCACAATCC TGATGAGGTG GCGCCTGCTG GTCGGTGAGC
  241 TGCGGGCCCT GCTTGGCACC GGTGACCGCA GGGTCTGCAG TATCCAGGGA CTCTGACATC
  301 TTCTCACAGA TCACGTCCAC CAGACGTTCA AACGTCTGCT TGACATTAAT GTTGTCCTTG
  361 GCGCTGGCCT CAAAGAACTC AAAGCCCAGG TGGTCAGCCA GCTGCCGGCC ACGCTCTGAG
  421 GACACCACTC GCTCATCTTC CATGTCACAC TTGTTTCCCA CCAGCAGCAC CTGGGCATTG
  481 TCCCACGAGT AAGTTTTGAT CTGAGTGGAC CAGTCCTGCA CTGCATTAAA TGACTCCTCA
  541 TTGGTGATGT CATACATTAG GATGAAGCCC ATGGCGCCTC GGTAATAGGC TGTGGTGATG
  601 GTGCGGTACC GCTCTTGCCC TGCTGTGTCC CAGATCTGCA GCTTGATCCT CTTGTCGTTG
  661 CGATAGATGG TTTTGACCTT GAAGTCTATG CNAACGGTGC TGACAAAGGC TGGAGTGAAG
  721 GANTCATCTG CGTAGCGGAA GANGAACGAN GTTTTGCCCA CGCTGCTGTT CNCNATGATC
  781 AGGATCTTGA ACATATAGTC GAANTTCTGG TCTGANGANT CCTTCTGCCN NTANCGAGAN
  841 TCTGTGGCGG AAGCNATGGN TCTGANTNCG GANTTGTNCA GCTCGTCCAN GCCGANANTG
  901 ATNCCNGNGG NNGNCACNAA NNNCNNCNNN ANCNTGTNAT NGCNCTTNNC NNTNGGGNNN
  961 NTNGCNCNNG NNNGNANNNG GNGNNCNNNN NNNNNNNNNN GNNNNCNNCN NNGGNNNNNN
 1021 NNNNNNNGGG GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NN
//
Primer: CMV_Forward
Region: CMV pro, EGFP 5'
Sequence file: RDB17240_A9Elc.seq check
>D06280C4_A9El_1_CMV-Forward_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCN TTGNNGCNAN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGANTTC GTGANCGCCG CCNGGATCAC TCTCGGCATG GACGANCTGT ACAAGTCCGG
  841 ANTCAGATCC ATGGCTTCCG CCACAGACTC TCGCTATGGG CAGAANGANT CCNCNNANCA
  901 GAACTTCNAC TATATGTTCN NNATNCNGAT CATNNGGAAC AGCANCGTGG GNAAAACCNC
  961 NTTCCNCTTN CNNNNNNCNN ANGANNCCNN CNNNNNNNCC NTTNNNANCN NNNNTNGNNN
 1021 NNNNNNNNNN NNNNNAAANC NNNNNNNNGN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NGNNNNNNNN
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB17240_A9Eld.seq check
>D06280C4_A9El_1_SV40pro_F_V2_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGN NNAGNNCGAT CNNGAGACAG GATGAGGNTC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA
  781 NCGCTTCCTC GTGCTTTACN GNATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCNTGAC NANTTCTTCT GANCNGGANT CTGGGGTTCG AAATGANCNA NNNAGCGANG
  901 CCCNANCTGN CNTCACGAGA TTTCNATTNC ACCGCNNCNN TCTATNAANN NTGGNNNTCN
  961 GNANNCNNTT TNCNNGNNNN NCNNNCNNGN NNNNNNNNNN NNNNNNGGGN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNCNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.01.09

GNP_filter3_RDBDEP_html_191130.pl