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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab3A (#RDB17240)

Expression vector of mouse Rab3A fused with EGFP.

Clone info. Expression vector of mouse Rab3A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab3a cDNA
Reference of insert sequence AB232587 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17240 pEGFP-C1-Rab3A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab3A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17240).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17240_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17240_A9Ela.seq check
>D06280C4_A9El_1_EGFP-C2_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCGNN CNCNTGGTCC TGCTGGANTT CGTGNCCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCTTCC GCCACAGACT
  121 CTCGCTATGG GCAGAAGGAG TCCTCAGACC AGAACTTCGA CTATATGTTC AAGATCCTGA
  181 TCATTGGGAA CAGCAGCGTG GGCAAAACCT CGTTCCTCTT CCGCTACGCA GATGACTCCT
  241 TCACTCCAGC CTTTGTCAGC ACCGTTGGCA TAGACTTCAA GGTCAAAACC ATCTATCGCA
  301 ACGACAAGAG GATCAAGCTG CAGATCTGGG ACACAGCAGG GCAAGAGCGG TACCGCACCA
  361 TCACCACAGC CTATTACCGA GGCGCCATGG GCTTCATCCT AATGTATGAC ATCACCAATG
  421 AGGAGTCATT TAATGCAGTG CAGGACTGGT CCACTCAGAT CAAAACTTAC TCGTGGGACA
  481 ATGCCCAGGT GCTGCTGGTG GGAAACAAGT GTGACATGGA AGATGAGCGA GTGGTGTCCT
  541 CAGAGCGTGG CCGGCAGCTG GCTGACCACC TGGGCTTTGA GTTCTTTGAG GCCAGCGCCA
  601 AGGACAACAT TAATGTCAAG CAGACGTTTG AACGTCTGGT GGACGTGATC TGTGAGAAGA
  661 TGTCAGAGTC CCTGGATACT GCAGACCCTG CGGTCACCGG TGCCAAGCAG GGCCCGCAGC
  721 TCACCGACCA GCANGCGCCA CCTCATCANG ATTGTGCCTG CTGAAATCAC TAGTGAATTC
  781 TGCAGTCGAC NGTACCGCGG GCCCNGGANC CACCGGATCT AGATAACTGA TCATAATCAG
  841 CCATACCACA TTTGTAGAGG NTTTACTTGC TTTAAAAAAC CTCCCNCACC TCCCCCTGAA
  901 NCTGAAACNT AAAATGAATG CAATTGTTGN TGTTAACTTG NTTANNGCAN CTTATNNNGG
961 NTACNAANAA AGCNNNANCN TCANNNNTTN NNNAATAANG CATTTTTTNN NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17240_A9Elb.seq check
>D06280C4_A9El_1_SV40polyA-R2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNANNAANN NGNNNNNNNN NANNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCAGCA GGCACAATCC TGATGAGGTG GCGCCTGCTG GTCGGTGAGC
  241 TGCGGGCCCT GCTTGGCACC GGTGACCGCA GGGTCTGCAG TATCCAGGGA CTCTGACATC
  301 TTCTCACAGA TCACGTCCAC CAGACGTTCA AACGTCTGCT TGACATTAAT GTTGTCCTTG
  361 GCGCTGGCCT CAAAGAACTC AAAGCCCAGG TGGTCAGCCA GCTGCCGGCC ACGCTCTGAG
  421 GACACCACTC GCTCATCTTC CATGTCACAC TTGTTTCCCA CCAGCAGCAC CTGGGCATTG
  481 TCCCACGAGT AAGTTTTGAT CTGAGTGGAC CAGTCCTGCA CTGCATTAAA TGACTCCTCA
  541 TTGGTGATGT CATACATTAG GATGAAGCCC ATGGCGCCTC GGTAATAGGC TGTGGTGATG
  601 GTGCGGTACC GCTCTTGCCC TGCTGTGTCC CAGATCTGCA GCTTGATCCT CTTGTCGTTG
  661 CGATAGATGG TTTTGACCTT GAAGTCTATG CNAACGGTGC TGACAAAGGC TGGAGTGAAG
  721 GANTCATCTG CGTAGCGGAA GANGAACGAN GTTTTGCCCA CGCTGCTGTT CNCNATGATC
  781 AGGATCTTGA ACATATAGTC GAANTTCTGG TCTGANGANT CCTTCTGCCN NTANCGAGAN
  841 TCTGTGGCGG AAGCNATGGN TCTGANTNCG GANTTGTNCA GCTCGTCCAN GCCGANANTG
  901 ATNCCNGNGG NNGNCACNAA NNNCNNCNNN ANCNTGTNAT NGCNCTTNNC NNTNGGGNNN
961 NTNGCNCNNG NNNGNANNNG GNGNNCNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17240_A9Elc.seq check
>D06280C4_A9El_1_CMV-Forward_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCN TTGNNGCNAN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGANTTC GTGANCGCCG CCNGGATCAC TCTCGGCATG GACGANCTGT ACAAGTCCGG
  841 ANTCAGATCC ATGGCTTCCG CCACAGACTC TCGCTATGGG CAGAANGANT CCNCNNANCA
  901 GAACTTCNAC TATATGTTCN NNATNCNGAT CATNNGGAAC AGCANCGTGG GNAAAACCNC
  961 NTTCCNCTTN CNNNNNNCNN ANGANNCCNN CNNNNNNNCC NTTNNNANCN NNNNTNGNNN
1021 NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17240_A9Eld.seq check
>D06280C4_A9El_1_SV40pro_F_V2_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGN NNAGNNCGAT CNNGAGACAG GATGAGGNTC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA
  781 NCGCTTCCTC GTGCTTTACN GNATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCNTGAC NANTTCTTCT GANCNGGANT CTGGGGTTCG AAATGANCNA NNNAGCGANG
  901 CCCNANCTGN CNTCACGAGA TTTCNATTNC ACCGCNNCNN TCTATNAANN NTGGNNNTCN
961 GNANNCNNTT TNCNNGNNNN NCNNNCNNGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]