Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-puro-HA-KIF13B

Retroviral expression vector of mouse KIF13B fused with HA.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Additional terms and conditions for distribution	Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
付加的提供条件	pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17235_B0Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F Region: pol, HA, insert 5' Sequence file: RDB17235_B0Fma.seq
>D6280B8_B0Fm_2_MMLV-pro_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGGN ATCGCANCTT GGNNACNCGC CGCCCNCGTG AAGGCTGCCG    61 ACCCCGGGGG TGGANNNTCC TCTAGACTGC CGGATCTCCA CCATGGGTTA CCCATACGAC   121 GTCCCAGACT ACGCTGGATC CATGGGAGAC TCCAAAGTGA AAGTGGCGGT ACGGGTCCGG   181 CCAATGAACC GACGAGAAAT TGACTTACAT ACCAAATGTG TGGTGGATGT TGAAGCGAAC   241 AAAGTTATTC TGAATCCTGT AAATACAAAT CTTTCCAAAG GAGATGCCCG GGGCCAGCCA   301 AAGATCTTTG CTTATGATCA CTGTTTCTGG TCCATGGATG AATCTGTCCG AGAGAAGTAT   361 GCAGGTCAAG AAGATGTTTT CAAGTGCCTT GGGGAGAACA TTCTTCAGAA TGCATTTGAT   421 GGCTACAACG CCTGTATCTT TGCTTATGGG CAGACAGGGT CTGGAAAGTC TTACACCATG   481 ATGGGCACTG CTGACCAACC TGGATTAATC CCAAGGCTCT GTAGTGGACT CTTTGAACGA   541 ACCCAGAAAG AGGAAAATGA AGAACAGAGT TTTAAAGTCG AAGTGTCGTA CATGGAAATT   601 TATAATGAAA AAGTCCGAGA CCTTCTTGAC CCCAAAGGAA GCCGTCAGAC GTTGAAAGTG   661 AGAGAGCACA GTGTGTTGGG ACCCTACGTG GATGGACTTT CTAAACTGGC TGTCACAAGC   721 TACAAGGATA TTGAGTCTCT GATGTCTGAN GGCAACAAGT CTCGGACAGT TGCTGCAACC   781 NACATGAACG ANGAAAGTAG CCGTTCNNNT GCAGNCTTCN AAATCNCCNN GACNCATACT   841 CTGNATGATG TGAANTCTGG GANATCTGGN NAGAAAGTNG GCAANCNNAN CTTANNNNNN   901 TNNNCNNNNA NNGANCGANC NNNNAAANNN NNNNCTGCNN NNNANAGGNN NNNNNAGGNN 961 ANCAACNTNN NNNNNNNCNN NNNNNNNN //
Primer: IRES-R Region: insert 3' Sequence file: RDB17235_B0Fmb.seq
>D06280B8_B0Fm_2_IRES-R_E06_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAG GGGGGGGGGC GGNNNTTACG TAGCGGCCGC    61 GAATTCGATT TTAGCTGGCC CAGGACTTCC GGTTCTCAGG ATTCTTGTAA CTCCTGTCTC   121 CCTTGGCCAG TGCAGCTGTC AGTGAAGCCA GGTTGGTGGC AGAGCCCGAG ATGGTAGCAC   181 TTCTGCGGAC CTCTGGGGCT CCCTGAGGCT GGAGCCCTGA ACTGCGCCTG CGCCCAGTGC   241 CCACAGCCCT GCGGACCCGG CTGGGCCTCA CCAGCAGCCC ATAGCCAGGG TTGCACCTGA   301 AATACTGTTT CCCACCAATG GAGCCATCAT TCTTTCCTGC AGGCAGATCC AGCTCCACAC   361 CAATCCATGT TCCCTCCTGG AAATCAGTTG GTCCAATATA CCGTACAATG CCCGTCTTGT   421 TGGTGCCCAC AACAACATAC TCTCCCTCTC TGAGCCACTC AGGGACTTCG CTGGCATCCT   481 CAGAGTCAGA TGTTACTTCT AGCCTCCCCA GAGTCTGTCC CCTAGCATTG CTGGGGTCTT   541 CTGAAGCCAG GGGGTCCTCC TCTGCTCCGG ANGAGGCACC ACCCAGCATG CCTGTGAAGG   601 ACTTAAGCTC TGANGTCCGC ACTTTCCGGA TTCTGAATGG GGACGTGGGT ACAGCAGACG   661 CGGGTTCAGA AAGAANAGGT GCTCTGGGAA CCTCCAGCTC TGTGCTTTGA GTAGGAGCTG   721 CAGGTACGTG GGCCTCCTCT GGNGCANGAN GANGGCTTAN TGTGCAGGAN ANGAAAGCCA   781 GCTCANGCTC TGNGNTGACN TGNCNCANGG CANGGGGGGA NNNANGCGTC NGNCTGNNNA 841 NGNCNGNGGN GNNNAGGCCN NANGNGAGTG TTTCTGANAN GNNNGCAGAN NNNNNNNNN //
Primer: MMLV psi_F Region: MMLV psi, gag, pol Sequence file: RDB17235_B0Fmc.seq
>D6280B8_B0Fm_2_MMLV psi_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT GATTTTTGNT TTCGGTTTGG GANCGAAGCC GCGCCGCGCG    61 TCTTGTCTGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC   121 TGAAAATATG GGCCCGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG   181 GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT   241 TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT   301 TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA   361 CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG   421 GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT   481 CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC   541 TTCTCTAGGC GCCCCCATAT GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT   601 AAACTTCCCT GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT   661 ACAGGCTCTC TACTTAGTCC AGCACGAAGT CTGGAGANCT CTGGCGGCAG CCTACCAAGA   721 ACAACTGGAC CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG   781 CCGACACCAG ACTAAGAACC TAGAACNTCG CTNGAAAGGA NCTTACACAG NCCTGCTGAN   841 CACCCCCACC GCCCTCNAAG TANACGGCAT CGCANCTNNN NNACNCGCCG CCCNCNNNAA   901 NGNNGNCNNN CCNNGGGGGN NGGANCNNNC NNNNNNNNGN CNNNNNNNCN NCNNNGNNNA 961 CCNNTACNNN NNNCNNNNNN NNNN //
Primer: IRES-F Region: IRES, PuroR Sequence file: RDB17235_B0Fmd.seq
>D06280B8_B0Fm_2_IRES-F_F06_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCNNNGTTA ANANAACGTC TAGGCCCCCC GAACCACGGG    61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA CCGAGTACAA   121 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC   181 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG   241 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG   301 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG   361 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC   421 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT   481 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT   541 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC   601 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC   661 CGAAGGANCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA   721 CCCGCAGCGC CCGACCNNNN GANCGCACGA NCCCATGGCT CGACNATAAA ATAAAANANT   781 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTANGTT TGGCAAGCTA   841 GCTTAAGTAN CGCNATTTTG CANGGCNTNN NAAAAATACN TNANNTNNNA ANNNAGAANT   901 TCANATNNAG GNCNNNANCN NATGGNANNN NCNGNNNNTG GGCNAANCNN NNNANCNNNN 961 GNNNANNNNN NNNNCNNNNC CNNGNNNNNN NNNN //
Primer: PuroR_F Region: PuroR, 3' LTR Sequence file: RDB17235_B0Fme.seq
>D06280B8_B0Fm_2_PuroR_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NGNNNGANGN NCGCGCACCT GGTGCATGAN CCGCAAGCCC    61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT   121 GGCTCGACGA TAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC   181 CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT   241 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT   301 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT   361 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA   421 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT   481 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC   541 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC   601 CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG   661 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG   721 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCNAA   781 ATTAATTTGG TTTTTTTTCT TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGNAT   841 CGGNCAACGC GCGGGGGANA GGCGGTTTGC GTATTGGGCG CNCTNCCGCT TNCTCGCNCA   901 CTNANTCGCT GCGNNCGGNC NNNCNGNNGC NGNGANCNGN NATCANCNCA CNCNANGGNG 961 GTANTACNNN NATCNNNNNN ANNNNNNGNN NNNNNNNN //
Primer: Amp_R Region: AmpR pro, CMV en, delU3 5' LTR Sequence file: RDB17235_B0Fmf.seq
>D6280B8_B0Fm_2_Amp_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTGN NGCANTNATN NGGGTTATTG TCTCNTGAGC GGATACATAT    61 TTGAATGTAT TTNNNNNAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA   181 CGAGGCCCTT TCGTCTTCAC TCGAGGAGCT TGGCCCATTG CATACGTTGT ATCCATATCA   241 TAATATGTAC ATTTATATTG GCTCATGTCC AACATTACCG CCATGTTGAC ATTGATTATT   301 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT   361 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC   421 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG   481 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT   541 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA   601 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT   661 TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGANCTGA AATGACCCTG   721 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC   781 CCGANCTCAA TAAAAGAGCC CACAACCCCT CANTCNGCGC GCCAGTCCTC CGATTGACTG   841 ANTCGCCCGG NNACCCGNGT ATCCNNTAAA CCCTCTNGCA NTTNCATNCN ANTTNNGGNN   901 NNGCNNTTCC TTGGGNNGNN NNNCNNNNAN NGNNTGNNTA CCNGNCNNCG GGGGNNNTTT 961 CNTTTGGGGG NNNCNNNNNG NNNNNGNNNN NCCNNNNCNN NGGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl