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pEGFP-C1-KIF13B-tail-deltaRHD (#RDB17233)

Expression vector of mouse KIF13B fused with EGFP. The KIF13B-tail mutant without a motor domain and the lacks an RHD, a Rab10-binding dificient mutant.

Clone info. Expression vector of mouse kinesin family member 13B (KIF13B) fused with EGFP at N-terminus. The KIF13B-tail mutant without a motor domain and the lacks an Rab10-binding homology domain (RHD), a Rab10-binding dificient mutant, see Fig. 4A and S4D of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Kif13b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17233 pEGFP-C1-KIF13B-tail-deltaRHD Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-KIF13B-tail-deltaRHD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17233).

Reference section:

Etoh, K., Fukuda, M., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

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References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]