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pMRX-puro-EGFP-Rab10

Retroviral expression vector of mouse Rab10, fused with EGFP.

Catalog number RDB17223
Resource name pMRX-puro-EGFP-Rab10
Clone info. Retroviral expression vector of mouse Rab10, fused with EGFP at N-terminus.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cell).
Gene/insert name mouse Rab10 cDNA
Reference of nucleotide sequence AB232604 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17223 pMRX-puro-EGFP-Rab10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17223_B0Gkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F
Region: Pol region, EGFP 5'
Sequence file: RDB17223_B0Gka.seq check
>17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC
   61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
  301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
  661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
  721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
  781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC
  841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN
  901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN
961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN
//
Primer: IRES-R
Region: insert, EGFP 3'
Sequence file: RDB17223_B0Gkb.seq check
>17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT
   61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT
  121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA
  181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT
  241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT
  301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT
  361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT
  421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC
  481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA
  541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG
  601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA
  661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
  721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG
  901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN
961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN
//
Primer: mVenus-M_F
Region: EGFP 3', insert 5'
Sequence file: RDB17223_B0Gkc.seq check
>17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
  241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT
  301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA
  361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA
  421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA
  481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC
  541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA
  601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT
  661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG
  721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG
  781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT
  841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA
  901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN
961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN
//
Primer: MMLV-pol_F
Region: Pol region, EGFP 5'
Sequence file: RDB17223_B0Gkd.seq check
>17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC
   61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
  301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
  661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
  721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
  781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC
  841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN
  901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN
961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN
//
Primer: IRES-R
Region: insert, EGFP 3'
Sequence file: RDB17223_B0Gke.seq check
>17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT
   61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT
  121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA
  181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT
  241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT
  301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT
  361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT
  421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC
  481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA
  541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG
  601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA
  661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
  721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG
  901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN
961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN
//
Primer: mVenus-M_F
Region: EGFP 3', insert 5'
Sequence file: RDB17223_B0Gkf.seq check
>17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
  241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT
  301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA
  361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA
  421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA
  481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC
  541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA
  601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT
  661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG
  721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG
  781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT
  841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA
  901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN
961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN
//
Primer: Amp_R
Region: AmpR pro, CMV en. Delta U3 5' UTR
Sequence file: RDB17223_B0Gkg.seq check
>17223_17223_B0Gk_Amp_R_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGANC ATTTATNNGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCACTCG AGGAGCTTGG CCCATTGCAT ACGTTGTATC CATATCATAA
  241 TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC
  301 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
  361 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
  421 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
  481 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
  541 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
  601 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
  661 CATGCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  781 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGANTGANT
  841 CGCCCGGNTN NNNGTGTATC CNATAAANCC TCTTGCAGTT GCATCCGANT NGNGGNCTCG
  901 CTGTNCCTNG GGANGGTCNN CTCTGANTGN NTNNNTACCC NNCAGCGGGG GNCTTNCATT
961 TNGGGGNNTC GNCCGGGNNC NGNNNANCCN NGCCCNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl