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pMRX-puro-EGFP-Rab10

Retroviral expression vector of mouse Rab10, fused with EGFP.

Catalog number RDB17223
Resource name pMRX-puro-EGFP-Rab10
Clone info. Retroviral expression vector of mouse Rab10, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cell).
Gene/insert name mouse Rab10 cDNA
Reference of insert sequence AB232604 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab10

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
          Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
          付加的提供条件 pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17223 pMRX-puro-EGFP-Rab10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17223_B0Gkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F
          Region: Pol region, EGFP 5'
          Sequence file: RDB17223_B0Gka.seq check
          >17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC
             61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA
            121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
            181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
            241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
            301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
            361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
            421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
            481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
            541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
            601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
            661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
            721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
            781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC
            841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN
            901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN
          961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN
          //
          Primer: IRES-R
          Region: insert, EGFP 3'
          Sequence file: RDB17223_B0Gkb.seq check
          >17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT
             61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT
            121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA
            181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT
            241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT
            301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT
            361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT
            421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC
            481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA
            541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG
            601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA
            661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
            721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT
            781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
            841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG
            901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN
          961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN
          //
          Primer: mVenus-M_F
          Region: EGFP 3', insert 5'
          Sequence file: RDB17223_B0Gkc.seq check
          >17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG
             61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
            121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
            181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
            241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT
            301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA
            361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA
            421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA
            481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC
            541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA
            601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT
            661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG
            721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG
            781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT
            841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA
            901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN
          961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN
          //
          Primer: MMLV-pol_F
          Region: Pol region, EGFP 5'
          Sequence file: RDB17223_B0Gkd.seq check
          >17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC
             61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA
            121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
            181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
            241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
            301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
            361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
            421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
            481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
            541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
            601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
            661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
            721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
            781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC
            841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN
            901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN
          961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN
          //
          Primer: IRES-R
          Region: insert, EGFP 3'
          Sequence file: RDB17223_B0Gke.seq check
          >17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT
             61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT
            121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA
            181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT
            241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT
            301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT
            361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT
            421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC
            481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA
            541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG
            601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA
            661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
            721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT
            781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
            841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG
            901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN
          961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN
          //
          Primer: mVenus-M_F
          Region: EGFP 3', insert 5'
          Sequence file: RDB17223_B0Gkf.seq check
          >17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG
             61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
            121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
            181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
            241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT
            301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA
            361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA
            421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA
            481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC
            541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA
            601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT
            661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG
            721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG
            781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT
            841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA
            901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN
          961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CMV en. Delta U3 5' UTR
          Sequence file: RDB17223_B0Gkg.seq check
          >17223_17223_B0Gk_Amp_R_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNTGANC ATTTATNNGG GTTATTGTCT CATGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
            181 GGCCCTTTCG TCTTCACTCG AGGAGCTTGG CCCATTGCAT ACGTTGTATC CATATCATAA
            241 TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC
            301 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
            361 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
            421 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
            481 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
            541 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
            601 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
            661 CATGCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
            721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
            781 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGANTGANT
            841 CGCCCGGNTN NNNGTGTATC CNATAAANCC TCTTGCAGTT GCATCCGANT NGNGGNCTCG
            901 CTGTNCCTNG GGANGGTCNN CTCTGANTGN NTNNNTACCC NNCAGCGGGG GNCTTNCATT
          961 TNGGGGNNTC GNCCGGGNNC NGNNNANCCN NGCCCNNGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl