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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmStr-C1-Rab10 (#RDB17221)

Expression vector of mouse Rab10, fused with mStr.

Clone info. Expression vector of mouse Rab10, fused with N-terminal mStr, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStrawberry-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab10 cDNA
Reference of insert sequence AB232604 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17221 pmStr-C1-Rab10 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmStr-C1-Rab10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17221).

Reference section:

Etoh, K., Fukuda, M., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17221_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F (pr0453)
Region: mStr 3', Insert 5'
Sequence file: RDB17221_A9Ela.seq check
>D06280A3_A9El_3_mCherry-C_F_B04_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCTG CCNGGCGCCT ACNTCGTCGG CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC TGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCGAAG AAGACGTACG
  181 ACCTGCTTTT CAAGCTGCTC CTGATCGGGG ACTCGGGAGT GGGCAAGACC TGCGTCCTTT
  241 TTCGTTTTTC GGACGATGCC TTCAATACCA CCTTTATTTC CACCATAGGA ATAGACTTTA
  301 AGATCAAAAC AGTGGAACTA CAAGGAAAGA AGATCAAGCT ACAGATATGG GACACAGCAG
  361 GCCAGGAGCG ATTTCACACC ATCACAACCT CCTACTACAG AGGAGCAATG GGCATCATGC
  421 TAGTGTATGA CATCACCAAC GGTAAAAGCT TTGAGAACAT CAGCAAGTGG CTTAGAAACA
  481 TAGATGAGCA TGCCAATGAA GATGTGGAAA GAATGTTACT AGGGAACAAG TGTGACATGG
  541 ACGACAAGAG AGTTGTACCG AAAGGCAAAG GAGAACAGAT TGCAAGGGAG CATGGTATTA
  601 GGTTTTTTGA GACTAGTGCA AAAGCAAATA TAAACATCGA AAAGGCGTTC CTCACATTAG
  661 CTGAAGACAT CCTCCGAAAG ACCCCTGTAA AAGAACCCAA CAGTGAAAAC GTAGATATCA
  721 GCAGTGGAGG AGGCGTGACG GGCTGGAAGA NCAAGTGCTG CTGAAATCAC TAGTGAANTC
  781 TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGNTCT AGATAACTGA TCATAATCAG
  841 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTNAAAAAC CTCCNACACN NNCCCCNGAA
  901 CCTGAAACAT AAAATGAANG NNATTGNNGT TGNTAACTTG NTTATTGNAG CTTATNANGG
  961 TTACNAANNA ANCNNNANCA TCNNNAATTT CNNNAANAAA GCNNTTTTTT TNNNNNNNNT
1021 NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: Insert 3'
Sequence file: RDB17221_A9Elb.seq check
>D06280A3_A9El_3_SV40polyA_R2_C04_03_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNGNNNNN NACAACNATT GCATTCATTT TATGTTTCAG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCACT AGTGATTTCA GCAGCACTTG CTCTTCCAGC CCGTCACGCC TCCTCCACTG
  241 CTGATATCTA CGTTTTCACT GTTGGGTTCT TTTACAGGGG TCTTTCGGAG GATGTCTTCA
  301 GCTAATGTGA GGAACGCCTT TTCGATGTTT ATATTTGCTT TTGCACTAGT CTCAAAAAAC
  361 CTAATACCAT GCTCCCTTGC AATCTGTTCT CCTTTGCCTT TCGGTACAAC TCTCTTGTCG
  421 TCCATGTCAC ACTTGTTCCC TAGTAACATT CTTTCCACAT CTTCATTGGC ATGCTCATCT
  481 ATGTTTCTAA GCCACTTGCT GATGTTCTCA AAGCTTTTAC CGTTGGTGAT GTCATACACT
  541 AGCATGATGC CCATTGCTCC TCTGTAGTAG GAGGTTGTGA TGGTGTGAAA TCGCTCCTGG
  601 CCTGCTGTGT CCCATATCTG TAGCTTGATC TTCTTTCCTT GTAGTTCCAC TGTTTTGATC
  661 TTAAAGTCTA TTCCTATGGT GGAAATAAAG GTGGTATTGA AGGCATCGTC CGAAAAACGA
  721 AAAAGGACGC AGGTCTTGCC CACTCCCGAN TCCCCGATCA GGANCAGCTT GAAAAGCANG
  781 TCGTACGTCT TCTTCGCCAT GGATCTGANT CCGGACTTGT ACAGCTCGTC CATGCCGCCG
  841 GTGGANTGNC GGCCCTCGGC GCGTTCGTAC AGTTCCACGA TGGTGTANNC CNCGTTGTGG
  901 GANGTGANGT NNAACTNGAT GCCNANNATG TNNNGCCNGG NANCTGNNNG GGNTTCTNNG
  961 NNNTTGTANG TGGNCTNGNN NNCANCGNCN NANNNNNNNN NGTNNNTCNN NTNNNNNNNN
1021 NN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, mStr 5'
Sequence file: RDB17221_A9Elc.seq check
>D06280A3_A9El_3_CMV_Forward_A04_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT NCGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGAATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT
  181 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
  241 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
  301 GGACATCCTA ACCCCCAACT TCACCTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
  361 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
  421 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
  481 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
  541 GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA
  601 GGGCGAGATC AAGATGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA
  661 GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACATCG TCGGCATCAA
  721 GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACTGTACG AACGCGCCGA
  781 GGGCCGCCAC TCCACCGGCG GCATGGNCGA NCTGTACAAG TCCGGANTCA GATCCATGGC
  841 GAANAAGANG TACGANCTGC TTTTCNAGCT GCNCCTGATC NGGGNNTCNG GANNGGGCAA
  901 GANCNGCNNC CTTTTTCGTT TTTNNNANNA NGCCNTCNNN ACCNCCNTTA TTTNCNNNNN
961 NNNANNNNAC NTNNNNANNN AAANANNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB17221_A9Eld.seq check
>D06280A3_A9El_3_SV40pro_ori_F_D04_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCC NCNNCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
  121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
  301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
  361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
  421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
  481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
  541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
  601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
  661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
  721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCAGGACATA
  781 GCGTTGGCTA CCCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
  841 GNGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG NNNTCNATCG CCTNCTTGAN
  901 GANTTCTTCT NANCGGGNNT CNGGGGGTTC NAAATGNNCN ANCNANCNAC NCCNNACNNG
  961 CCNTCNCNAN NTTNCNANNN NNNNNNCNNN CNTNNNNNNN ANNNNNNGNN NTNNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]