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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmStr-C1-Rab10

Expression vector of mouse Rab10, fused with mStr.

Catalog number RDB17221
Resource name pmStr-C1-Rab10
Clone info. Expression vector of mouse Rab10, fused with N-terminal mStr, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStrawberry-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab10 cDNA
Reference of nucleotide sequence AB232604 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab10 (NCBI Gene 19325) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17221 pmStr-C1-Rab10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17221_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F
Region: mStr 3', Insert 5'
Sequence file: RDB17221_A9Ela.seq check
>D06280A3_A9El_3_mCherry-C_F_B04_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCTG CCNGGCGCCT ACNTCGTCGG CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC TGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCGAAG AAGACGTACG
  181 ACCTGCTTTT CAAGCTGCTC CTGATCGGGG ACTCGGGAGT GGGCAAGACC TGCGTCCTTT
  241 TTCGTTTTTC GGACGATGCC TTCAATACCA CCTTTATTTC CACCATAGGA ATAGACTTTA
  301 AGATCAAAAC AGTGGAACTA CAAGGAAAGA AGATCAAGCT ACAGATATGG GACACAGCAG
  361 GCCAGGAGCG ATTTCACACC ATCACAACCT CCTACTACAG AGGAGCAATG GGCATCATGC
  421 TAGTGTATGA CATCACCAAC GGTAAAAGCT TTGAGAACAT CAGCAAGTGG CTTAGAAACA
  481 TAGATGAGCA TGCCAATGAA GATGTGGAAA GAATGTTACT AGGGAACAAG TGTGACATGG
  541 ACGACAAGAG AGTTGTACCG AAAGGCAAAG GAGAACAGAT TGCAAGGGAG CATGGTATTA
  601 GGTTTTTTGA GACTAGTGCA AAAGCAAATA TAAACATCGA AAAGGCGTTC CTCACATTAG
  661 CTGAAGACAT CCTCCGAAAG ACCCCTGTAA AAGAACCCAA CAGTGAAAAC GTAGATATCA
  721 GCAGTGGAGG AGGCGTGACG GGCTGGAAGA NCAAGTGCTG CTGAAATCAC TAGTGAANTC
  781 TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGNTCT AGATAACTGA TCATAATCAG
  841 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTNAAAAAC CTCCNACACN NNCCCCNGAA
  901 CCTGAAACAT AAAATGAANG NNATTGNNGT TGNTAACTTG NTTATTGNAG CTTATNANGG
  961 TTACNAANNA ANCNNNANCA TCNNNAATTT CNNNAANAAA GCNNTTTTTT TNNNNNNNNT
1021 NNNNNNNNNN
//
Primer: SV40polyA_R2
Region: Insert 3'
Sequence file: RDB17221_A9Elb.seq check
>D06280A3_A9El_3_SV40polyA_R2_C04_03_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNGNNNNN NACAACNATT GCATTCATTT TATGTTTCAG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCACT AGTGATTTCA GCAGCACTTG CTCTTCCAGC CCGTCACGCC TCCTCCACTG
  241 CTGATATCTA CGTTTTCACT GTTGGGTTCT TTTACAGGGG TCTTTCGGAG GATGTCTTCA
  301 GCTAATGTGA GGAACGCCTT TTCGATGTTT ATATTTGCTT TTGCACTAGT CTCAAAAAAC
  361 CTAATACCAT GCTCCCTTGC AATCTGTTCT CCTTTGCCTT TCGGTACAAC TCTCTTGTCG
  421 TCCATGTCAC ACTTGTTCCC TAGTAACATT CTTTCCACAT CTTCATTGGC ATGCTCATCT
  481 ATGTTTCTAA GCCACTTGCT GATGTTCTCA AAGCTTTTAC CGTTGGTGAT GTCATACACT
  541 AGCATGATGC CCATTGCTCC TCTGTAGTAG GAGGTTGTGA TGGTGTGAAA TCGCTCCTGG
  601 CCTGCTGTGT CCCATATCTG TAGCTTGATC TTCTTTCCTT GTAGTTCCAC TGTTTTGATC
  661 TTAAAGTCTA TTCCTATGGT GGAAATAAAG GTGGTATTGA AGGCATCGTC CGAAAAACGA
  721 AAAAGGACGC AGGTCTTGCC CACTCCCGAN TCCCCGATCA GGANCAGCTT GAAAAGCANG
  781 TCGTACGTCT TCTTCGCCAT GGATCTGANT CCGGACTTGT ACAGCTCGTC CATGCCGCCG
  841 GTGGANTGNC GGCCCTCGGC GCGTTCGTAC AGTTCCACGA TGGTGTANNC CNCGTTGTGG
  901 GANGTGANGT NNAACTNGAT GCCNANNATG TNNNGCCNGG NANCTGNNNG GGNTTCTNNG
  961 NNNTTGTANG TGGNCTNGNN NNCANCGNCN NANNNNNNNN NGTNNNTCNN NTNNNNNNNN
1021 NN
//
Primer: CMV_Forward
Region: CMV pro, mStr 5'
Sequence file: RDB17221_A9Elc.seq check
>D06280A3_A9El_3_CMV_Forward_A04_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT NCGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGAATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT
  181 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
  241 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
  301 GGACATCCTA ACCCCCAACT TCACCTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
  361 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
  421 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
  481 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
  541 GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA
  601 GGGCGAGATC AAGATGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA
  661 GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACATCG TCGGCATCAA
  721 GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACTGTACG AACGCGCCGA
  781 GGGCCGCCAC TCCACCGGCG GCATGGNCGA NCTGTACAAG TCCGGANTCA GATCCATGGC
  841 GAANAAGANG TACGANCTGC TTTTCNAGCT GCNCCTGATC NGGGNNTCNG GANNGGGCAA
  901 GANCNGCNNC CTTTTTCGTT TTTNNNANNA NGCCNTCNNN ACCNCCNTTA TTTNCNNNNN
961 NNNANNNNAC NTNNNNANNN AAANANNNNN NNNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB17221_A9Eld.seq check
>D06280A3_A9El_3_SV40pro_ori_F_D04_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCC NCNNCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
  121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
  301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
  361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
  421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
  481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
  541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
  601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
  661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
  721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCAGGACATA
  781 GCGTTGGCTA CCCGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
  841 GNGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG NNNTCNATCG CCTNCTTGAN
  901 GANTTCTTCT NANCGGGNNT CNGGGGGTTC NAAATGNNCN ANCNANCNAC NCCNNACNNG
  961 CCNTCNCNAN NTTNCNANNN NNNNNNCNNN CNTNNNNNNN ANNNNNNGNN NTNNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.11.04

GNP_filter3_RDBDEP_html_201104.pl