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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human TICAM-2 C HA expression vector (#RDB16928)

Expression vector of human TICAM-2 tagged with HA at C-terminus.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 2 (TICAM-2) tagged with HA at C-terminus.
Comment The full-length human TICAM-2 cDNA was obtained from MRC-5 cDNA by RT-PCR. Expession was confirmed by the depositor.
Vector backbone pCMV-Flag (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM2 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

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RDB16928 human TICAM-2 C HA expression vector DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-2 C HA expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16928).

Reference section:

Oshiumi, H., Sasai, M., Shida, K., Fujita, T., Matsumoto, M., Seya, T., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16928_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a_F2 ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16928_A9B7a.seq check
>D06030A6_A9A7_2_EF1a_F2_B02_02_ABI08.ab1
    1 AGGCGGCCCG GGGTGCTGGC CTCGCGCCGT CGTGTATCGC CCCGCCCTGG GCGGCAAGGC
   61 TGGTCTGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC CCTGCTGCAG
  121 GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA CCCACACAAA
  181 GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC
  241 CGTCCAGGCA CCTCGATTAG TTCTCGTGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG
  301 AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG
  361 CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA
  421 TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA
  481 TTCTCTAGAG ATCCCTCGAC CTCGAGCCAC CATGGGTATC GGGAAGTCTA AAATAAATTC
  541 CTGCCCTCTT TCTCTCTCTT GGGGTAAAAG GCACAGTGTG GATACAAGTC CAGGATATCA
  601 TGAGTCAGAT TCCAAGAAGT CTGAAGATCT ATCCTTGTGT AATGTTGCTG AGCACAGCAA
  661 TACAACAGAG GGGCCAACAG GAAAGCAGGA GGGAGCTCAG AGCGTGGAAG AGATGTTTGA
  721 AGAAGAAGCT GAAGAAGAGG TGTTCCTCAA ATTTGTGATA TTGCATGCAG AAGATGACAC
  781 AGATGAAGCC CTCAGAGTCC AGAATCTGCT ACAAGATGAC TTTGGTATCA AACCCGGAAT
  841 AATCTTTGCT GAGATGCCAT GTGGCAGACA GCATTTACAG AATTTAGATG ATGCTGTAAA
  901 TGGGTCTGCA TGGACAATCT TATTACTGAC TGAAAACTTT TTTAAGAGAT ACTTGGTGTA
  961 ATTTCCAGTT CTATACGTCC CTAATGAACT CCGTTAACAG GCAGCATAAA TACAACTCTG
 1021 TTATACCCAT GCGGCCCCTG AAACAATCCC CTTTCCCCGA GAAAGACTCC CTTTTGCCTC
 1081 TAAACATCCA TGCCTTTAGA GGAAGAAGTC GTTGAATTCG TACACAAGCA GAAAGATTTT
1141 TTCACAGGAG TCTCGTGGTG TGAATA
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), HA, insert 3'
Sequence file: RDB16928_A9B7b.seq check
>D06030A6_A9A7_2_hGCSF_R_E01_05_ABI08.ab1
    1 GGGGGGGATG ATCTCTCAAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGT CGCGGCCGCT
   61 CAAGCGTAAT CTGGAACATC GTATGGGTAG GCAATAAATT GTCTTTGTAC CATATTTCTT
  121 GTCTCTTTCC ATATAGTTTG TTGTGTCTTA TACACAGACT CCTGAAAAAT TCTTTCTACT
  181 TGTGTAGGAA ATCCACGACT TTCTTCCTCT AAGGCATTGA TGGTTTGGAG GGCAAAGGGA
  241 GTCCTTTCTC GGGGAAGGGG ATTGTTCAGG GGCCGCATGG GTATAACAGA GTTGTATTTA
  301 TGCTGCCTGT TAACGGAGTT CATTAGGGAC GTATAGAACT GGAAATTACA CCAAGTATCT
  361 CTTAAAAAGT TTTCAGTCAG TAATAAGATT GTCCATGCAG ACCCATTTAC AGCATCATCT
  421 AAATTCTGTA AATGCTGTCT GCCACATGGC ATCTCAGCAA AGATTATTCC GGGTTTGATA
  481 CCAAAGTCAT CTTGTAGCAG ATTCTGGACT CTGAGGGCTT CATCTGTGTC ATCTTCTGCA
  541 TGCAATATCA CAAATTTGAG GAACACCTCT TCTTCAGCTT CTTCTTCAAA CATCTCTTCC
  601 ACGCTCTGAG CTCCCTCCTG CTTTCCTGTT GGCCCCTCTG TTGTATTGCT GTGCTCAGCA
  661 ACATTACACA AGGATAGATC TTCAGACTTC TTGGAATCTG ACTCATGATA TCCTGGACTT
  721 GTATCCACAC TGTGCCTTTT ACCCCAAGAG AGAGAAAGAG GGCAGGAATT TATTTTAGAC
  781 TTCCCGATAC CCATGGTGGC TCGAGGTCGA GGGATCTCTA GAGAATTCCT CACGACACCT
  841 GAAATGGAAG AAAAAAACTT TGAACCACTG TCTGAGGCTT GAGAATGAAC CAAGATCCAA
  901 ACTCAAAAAG GGCAAATTCC AAGGAGAATT ACATCAAGTG CCAAGCTGGC CTAACTTCAG
  961 TCTCCACCCA CTCAGTGTGG GGAAACTCCA TCGCATAAAA CCCCTCCCCC CAACCCTAAA
 1021 GACGACGTAC TCCAAAAGCA CGAGAAACTA AATCGAGGTG CCTGGACGCG CCCGGGTACT
 1081 CCGTGAGTCA CATGAGCGAC GCTGAGAACC GGAAAGGCCG TTTTCTTGTG TGGTGACTCA
1141 CCCGACCGGC TCTCCCGGAA GGCGGTC
//
Primer: SV40pro_F_V2 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16928_A9B7c.seq check
>D06030A6_A9A7_2_SV40pro_F_V2_D02_11_ABI24.ab1
    1 TGGGACTTTT TTTGGGGGGC CTAGGCTTTT GCAAAAGCTT TGCAAAGATG GATAAAGTTT
   61 TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT
  121 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG
  181 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT
  241 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA
  301 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC
  361 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT
  421 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG
  481 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG
  541 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT
  601 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG
  661 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC
  721 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA
  781 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT
  841 GGCCTCGCGC CGTCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGTCTG GTCGGCACCA
  901 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CATGGAGCTC AAAATGGAAG
  961 GACGCGGCGC TCGGGAGAGC GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGCCTTTCCG
 1021 TCCTCAGCCG TCGCTTCATG TGAACTCCAC GAGTACCTGG CGACCGATCC AGACACTTCG
1081 ATTAGGTTTC CTCGTTGGCC CCTTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]