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human TICAM-1 C HA expression vector (#RDB16927)

Expression vector of human TICAM-1 tagged with HA at C-terminus.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 1 (TICAM-1) tagged with HA at C-terminus.
Comment The full-length human TICAM-1 was cloned from mRNA of the human fibroblast cell line MRC-5. Expession was confirmed by the depositor.
Vector backbone pCMV-Flag (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM1 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16927 human TICAM-1 C HA expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-1 C HA expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16927).

Reference section:

Oshiumi, H., Matsumoto, M., Funami, K., Akazawa, T., Seya, T., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16927_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a_F2 ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16927_A9B7a.seq check
>D06030A5_A9A7_2_EF1a_F2_G01_07_ABI08.ab1
    1 ACAGCGGGCC CGGGTGCTGG CCTCGCGCCG TCGTGTATCG CCCCGCCCTG GGCGGCAAGG
   61 CTGGTCTGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA
  121 GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA
  181 AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG
  241 CCGTCCAGGC ACCTCGATTA GTTCTCGTGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG
  301 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA
  361 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC
  421 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA
  481 ATTCTCTAGA GATCCCTCGA CCTCGAGTCA CCACCTCCAG CTTCTGCTGC CGGAGGCTGC
  541 ACCCACCTGT GCCCATGGCC TGCACGGGCC CATCACTTCC TAGCGCCTTC GACATTCTAG
  601 GTGCAGCAGG CCAGGACAAG CTCTTGTATC TGAAGCACAA ACTGAAGACC CCACGCCCAG
  661 GCTGCCAGGG GCAGGACCTC CTGCATGCCA TGGTTCTCCT GAAGCTGGGC CAGGAAACTG
  721 AGGCCAGGAT CTCTCTAGAG GCATTGAAGG CCGATGCGGT GGCCCGGCTG GTGGCCCGCC
  781 AGTGGGCTGG CGTGGACAGC ACCGAGGACC CAGAGGAGCC CCCAGATGTG TCCTGGGCTG
  841 TGGCCCGCTT GTACCACCTG CTGGCTGAGG AGAAGCTGTG CCCCGCCTCG CTGCGGGACG
  901 TGGCCTACCA GGAAGCCGTC CGCACCCTCA GCTCCAGGGA CGACCACCGG CTGGGGGGAA
  961 CTTCAGGATG AGGCCCGAAA CCGGGTGTGG GTGGGACATT GCTGGGGATC CAGGGGAGCA
 1021 TCCGGACGCT CCCAGTCCAA ATCTGGGCTG CCCTCCCACC ATCCTCGGCT TTTGCCCCTC
 1081 TGGGAACCAA GAAGCACCCA CCGCCCCCAT TTGACGATGA TTCCGACCTG AGCCCAAGGG
1141 ATGCTCCCTT GGCGATTCCC ACTGGACCAG GCCCCTGTGT GCT
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), HA, insert 3'
Sequence file: RDB16927_A9B7b.seq check
>D06030A5_A9A7_2_hGCSF_R_D01_04_ABI08.ab1
    1 CGGGGGAAAT GATCTCTCAA GGGTCCCAGG TGGGGACCCT CACTCTAGAG TCGCGGCCGC
   61 GGGAATTCGA TTCGCTAAGC GTAATCTGGT ACGTCGTATG GGTATTCTGC CTCCTGCGTC
  121 TTGTCCTCGG GCGCCTGGGA CCCTCTCTGG TTCCACATGT GGTTGTTCAG CCCCAGCTGT
  181 ACCATCTGTG CGTGGTGGAT AATGAGGGGT TGCAGCCCTG GGCTCTGAGG GGGTGCGGGT
  241 GAGGCCGTAG GGAAGGCTGG GGACTGCGGG AAGGGCAGTG ACTGTGGAAA GGCTGCTGGC
  301 TGTGGGGAGG GCGGTGGGGG GGTGCCAGCC TGCCATGCGT GCAGGGGTGG CGGCTGCGGG
  361 CACCCCGGCC AAGTGGGAAA GGGTGGCGGG GCTCCCAGGG GCACCTGGCC CCCAAAGGGC
  421 ATTCGAGCCC CATAGGGCGC CCCAGTCCCA AATGACATGT GGCTCCCAAA AGCCACCTGG
  481 AGCTGCTCCA TCTGTGCCTG GTAGGACAAG TAGCTCTGGA GGTAGGCTGA GTAGGCTGCG
  541 TTCAGTGCCG CCGCCTGCAT CCGCTCACCA TCCAGGTGTT GGCTCTGTTC CCGCAGGGCT
  601 CGGGTGTCCT GTTCCTTCCT CCACATGGCC TTTCGGGCCT GAAGCCTGTG GGGCTTGAAG
  661 GTGTTGGCCA CCTTCCTGGC GAAGATCTGG GAGTGTTCGT CCAGCCGCAC CAGCCCGGAT
  721 CATCAGGCTG GCCGTGTCGG AGCTGAGCTG GGCCGGGGAG CTCTCCAGGG GCAGGAAGGG
  781 GATGACACAG TCTGGCGACC CCTGTCGCGT GAGGTTGCTC ATCATGGCTT GGTTCACCTG
  841 GTGCAGCTCA GGCGACAGTC GAAGTTGGGA GGTGAGAAGT AGGATGATGA AAGCTGAGTG
  901 GTCTATGGCG TCCTGCAGGC AGCTCAGCTC CCCGCGCCCC GGGCACCTGG AAATCCTCGC
  961 AGAAGGTGGC CCCGTCGGGC ACGCCCAAGG GCCCTCCAGC TTCTCCGAAC CCGCAGGGCG
 1021 ATTGTGTTCG TCTGCCCCTG GCGTGAAGGA TCACAAAGTT ATAGAATTTC TGGTTCCGAT
 1081 GATGATTCCA GGGAAGACGG AAACATCGTA GGAAGGGTCA AGTGAGCCTT GACCAGAAGT
1141 AAGAATGAAT GAAGGAGTAG CATGGCATGA TGAATAG
//
Primer: SV40pro_F_V2 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16927_A9B7c.seq check
>D06030A5_A9A7_2_SV40pro_F_V2_A02_02_ABI24.ab1
    1 TCCGACCTTT TTTGGGAGGC CTAGGCTTTT GCAAAAAGCT TTGCAAAGAT GGATAAAGTT
   61 TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG GAGTGGGAAT
  121 TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG
  181 GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG
  241 TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC
  301 AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC
  361 CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT
  421 TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG
  481 GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG
  541 GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC
  601 TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG
  661 GCAAGATAGT CTTGTAAATG CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC
  721 CGCGGGCGGC GACGGGGCCC GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG
  781 AGCGCGGCCA CCGAGAATCG GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC
  841 TGGCCTCGCG CCGTCGTGTA TCGCCCCGCC CTGGGCGGCA AGGCTGGTCT GGTCGGCACC
  901 AGTTGCGTGA GCGGGAAGAT GGCCGCTTCC CGGCCCTGCT GCAGGAGCTC AAAATGGGAG
  961 GACGCGGCGC TCGGGAGAGC GGGCGGGTGG AGTCACCCAC ACAAGAAAAG GCCCTTTCGT
 1021 CTTCAGCCGT CGCTTCATGT GACTCCAACG AGTAACCGGC GTCGTCCAGC ACTTCGATAG
1081 TTCTCGATGT CTTTTGGAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]