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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human TICAM-2 C Flag expression vector (#RDB16926)

Expression vector of human TICAM-2 tagged with FLAG at C-terminus.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 2 (TICAM-2) tagged with FLAG at C-terminus.
Comment The full-length human TICAM-2 cDNA was obtained from MRC-5 cDNA by RT-PCR. Expession was confirmed by the depositor.
Vector backbone pCMV-Flag (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM2 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

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RDB16926 human TICAM-2 C Flag expression vector DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-2 C Flag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16926).

Reference section:

Oshiumi, H., Sasai, M., Shida, K., Fujita, T., Matsumoto, M., Seya, T., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16926_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a_F2 ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16926_A9B7a.seq check
>D06030A4_A9A7_3_EF1a_F2_D01_04_ABI08.ab1
    1 AGGCGGGCCT GGGGTGCTGG CCTCGCGCCG TCGTGTATCG CCCCGCCCTG GGCGGCAAGG
   61 CTGGTCTGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA
  121 GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA
  181 AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG
  241 CCGTCCAGGC ACCTCGATTA GTTCTCGTGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG
  301 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA
  361 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC
  421 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA
  481 ATTCTCTAGA GATCCCTCGA CCTCGAGCCA CCATGGGTAT CGGGAAGTCT AAAATAAATT
  541 CCTGCCCTCT TTCTCTCTCT TGGGGTAAAA GGCACAGTGT GGATACAAGT CCAGGATATC
  601 ATGAGTCAGA TTCCAAGAAG TCTGAAGATC TATCCTTGTG TAATGTTGCT GAGCACAGCA
  661 ATACAACAGA GGGGCCAACA GGAAAGCAGG AGGGAGCTCA GAGCGTGGAA GAGATGTTTG
  721 AAGAAGAAGC TGAAGAAGAG GTGTTCCTCA AATTTGTGAT ATTGCATGCA GAAGATGACA
  781 CAGATGAAGC CCTCAGAGTC CAGAATCTGC TACAAGATGA CTTTGGTATC AAACCCGGAA
  841 TAATCTTTGC TGAGATGCCA TGTGGCAGAC AGCATTTACA GAATTTAGAT GATGCTGTAA
  901 ATGGGTCTGC ATGGACAATC TTATTACTGA CTGAAAACTT TTTAAGAGAT ACTTGGTGTA
  961 ATTTCCAGTT CTATACGTCC CTAATGAACT CCGTTAACAG GCAGCATAAA TACAACTCTG
 1021 TTATACCCAT GCGGCCCCTG AACAATCCCC TTTCCCCGAG AAAGGACTCC CTTTGCCCTC
 1081 CAACATCCAT GCCTTTAGAG GAAGAAGTCA TGGATTCCTA CCAAAGTAGA AAAGAATTTT
1141 TTCCAGGAAG GTTCTGGTGG TTATTAAGG
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), FLAG, insert 3'
Sequence file: RDB16926_A9B7b.seq check
>D06030A4_A9A7_3_hGCSF_R_C01_03_ABI08.ab1
    1 GGGGGGAAAT GAATCTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAGA GTCGCGGCCG
   61 CTCACTTGTC ATCGTCGTCC TTGTAGTCGG CAATAAATTG TCTTTGTACC ATATTTCTTG
  121 TCTCTTTCCA TATAGTTTGT TGTGTCTTAT ACACAGACTC CTGAAAAATT CTTTCTACTT
  181 GTGTAGGAAA TCCACGACTT TCTTCCTCTA AGGCATTGAT GGTTTGGAGG GCAAAGGGAG
  241 TCCTTTCTCG GGGAAGGGGA TTGTTCAGGG GCCGCATGGG TATAACAGAG TTGTATTTAT
  301 GCTGCCTGTT AACGGAGTTC ATTAGGGACG TATAGAACTG GAAATTACAC CAAGTATCTC
  361 TTAAAAAGTT TTCAGTCAGT AATAAGATTG TCCATGCAGA CCCATTTACA GCATCATCTA
  421 AATTCTGTAA ATGCTGTCTG CCACATGGCA TCTCAGCAAA GATTATTCCG GGTTTGATAC
  481 CAAAGTCATC TTGTAGCAGA TTCTGGACTC TGAGGGCTTC ATCTGTGTCA TCTTCTGCAT
  541 GCAATATCAC AAATTTGAGG AACACCTCTT CTTCAGCTTC TTCTTCAAAC ATCTCTTCCA
  601 CGCTCTGAGC TCCCTCCTGC TTTCCTGTTG GCCCCTCTGT TGTATTGCTG TGCTCAGCAA
  661 CATTACACAA GGATAGATCT TCAGACTTCT TGGAATCTGA CTCATGATAT CCTGGACTTG
  721 TATCCACACT GTGCCTTTTA CCCCAAGAGA GAGAAAGAGG GCAGGAATTT ATTTTAGACT
  781 TCCCGATACC CATGGTGGCT CGAGGTCGAG GGATCTCTAG AGAATTCCTC ACGACACCTG
  841 AAATGGAAGA AAAAAAACTT TGAACCACTG TCTGAGGGCT TGAGAATGAA CCAAAGATCC
  901 AAACTCAAAA AGGGCAAATT CCAAGGAGAA ATTACATCAA GTGCCCAAGC TGGCCTAACT
  961 TCAGTCTCCA CCCACTCAGT GTGGGGAAAC TCCATCGCAT AAACCCCTCC CCCCCAACCT
 1021 AAAGACGACG TACTCCAAAA GCACGAGAAC TAATCGAGGT GCCTGGACGC GCCCGGTACT
 1081 CCGTGAGTCA CAATGAAAGC GACGGCTGAG AACGGAAAGC CTATCTTGGG TGGGGTGGAC
1141 TCACCCCGGT CCGGCCTTCC TTCCCCTGAA GGCGGC
//
Primer: pT2KXIG#1 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16926_A9B7c.seq check
>D06030A4_A9A7_3_SV40pro_F_V2_F01_16_ABI24.ab1
    1 TACAACCTTT TTTGGAGCCT AGGCTTTTGC AAAAGCTTTG CAAAGATGGA TAAAGTTTTA
   61 AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC TTGAAAGGAG TGGGAATTGG
  121 CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG
  181 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA
  241 TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT
  301 AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT
  361 GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC
  421 TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA
  481 GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC
  541 TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT
  601 CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA
  661 AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC
  721 GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC
  781 GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG
  841 CCTCGCGCCG TCGTGTATCG CCCCGCCCTG GCGGCAAAGG CTGGTCTGGT CGGCACCAGT
  901 TGCGTGAGCG GAAAGATGGC CGCTTCCCGG GCCCTGCTGC AGGGAGCTCA AAATGGGAGG
  961 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCCACA CAAAGGAAAA GACCTTTCCC
 1021 GTCCTCAGCC CGTCGCTCCA TGTGACTTCC AACGGAGTAC CGGACGTCGA TCTAGCACCT
1081 TCGATTAGTT CTCCGTTGCC TTTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]