Please review the QC test results indicated by icon below as well as clone information before placing your order.

human TICAM-2 C Flag expression vector

Expression vector of human TICAM-2 tagged with FLAG at C-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16926_A9B7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a_F2 Region: EF-1a pro, insert 5' Sequence file: RDB16926_A9B7a.seq
>D06030A4_A9A7_3_EF1a_F2_D01_04_ABI08.ab1     1 AGGCGGGCCT GGGGTGCTGG CCTCGCGCCG TCGTGTATCG CCCCGCCCTG GGCGGCAAGG    61 CTGGTCTGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA   121 GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA   181 AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG   241 CCGTCCAGGC ACCTCGATTA GTTCTCGTGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG   301 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA   361 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC   421 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA   481 ATTCTCTAGA GATCCCTCGA CCTCGAGCCA CCATGGGTAT CGGGAAGTCT AAAATAAATT   541 CCTGCCCTCT TTCTCTCTCT TGGGGTAAAA GGCACAGTGT GGATACAAGT CCAGGATATC   601 ATGAGTCAGA TTCCAAGAAG TCTGAAGATC TATCCTTGTG TAATGTTGCT GAGCACAGCA   661 ATACAACAGA GGGGCCAACA GGAAAGCAGG AGGGAGCTCA GAGCGTGGAA GAGATGTTTG   721 AAGAAGAAGC TGAAGAAGAG GTGTTCCTCA AATTTGTGAT ATTGCATGCA GAAGATGACA   781 CAGATGAAGC CCTCAGAGTC CAGAATCTGC TACAAGATGA CTTTGGTATC AAACCCGGAA   841 TAATCTTTGC TGAGATGCCA TGTGGCAGAC AGCATTTACA GAATTTAGAT GATGCTGTAA   901 ATGGGTCTGC ATGGACAATC TTATTACTGA CTGAAAACTT TTTAAGAGAT ACTTGGTGTA   961 ATTTCCAGTT CTATACGTCC CTAATGAACT CCGTTAACAG GCAGCATAAA TACAACTCTG  1021 TTATACCCAT GCGGCCCCTG AACAATCCCC TTTCCCCGAG AAAGGACTCC CTTTGCCCTC  1081 CAACATCCAT GCCTTTAGAG GAAGAAGTCA TGGATTCCTA CCAAAGTAGA AAAGAATTTT 1141 TTCCAGGAAG GTTCTGGTGG TTATTAAGG //
Primer: hGCSF_R Region: hGCSF 3'LTR + poly(A), FLAG, insert 3' Sequence file: RDB16926_A9B7b.seq
>D06030A4_A9A7_3_hGCSF_R_C01_03_ABI08.ab1     1 GGGGGGAAAT GAATCTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAGA GTCGCGGCCG    61 CTCACTTGTC ATCGTCGTCC TTGTAGTCGG CAATAAATTG TCTTTGTACC ATATTTCTTG   121 TCTCTTTCCA TATAGTTTGT TGTGTCTTAT ACACAGACTC CTGAAAAATT CTTTCTACTT   181 GTGTAGGAAA TCCACGACTT TCTTCCTCTA AGGCATTGAT GGTTTGGAGG GCAAAGGGAG   241 TCCTTTCTCG GGGAAGGGGA TTGTTCAGGG GCCGCATGGG TATAACAGAG TTGTATTTAT   301 GCTGCCTGTT AACGGAGTTC ATTAGGGACG TATAGAACTG GAAATTACAC CAAGTATCTC   361 TTAAAAAGTT TTCAGTCAGT AATAAGATTG TCCATGCAGA CCCATTTACA GCATCATCTA   421 AATTCTGTAA ATGCTGTCTG CCACATGGCA TCTCAGCAAA GATTATTCCG GGTTTGATAC   481 CAAAGTCATC TTGTAGCAGA TTCTGGACTC TGAGGGCTTC ATCTGTGTCA TCTTCTGCAT   541 GCAATATCAC AAATTTGAGG AACACCTCTT CTTCAGCTTC TTCTTCAAAC ATCTCTTCCA   601 CGCTCTGAGC TCCCTCCTGC TTTCCTGTTG GCCCCTCTGT TGTATTGCTG TGCTCAGCAA   661 CATTACACAA GGATAGATCT TCAGACTTCT TGGAATCTGA CTCATGATAT CCTGGACTTG   721 TATCCACACT GTGCCTTTTA CCCCAAGAGA GAGAAAGAGG GCAGGAATTT ATTTTAGACT   781 TCCCGATACC CATGGTGGCT CGAGGTCGAG GGATCTCTAG AGAATTCCTC ACGACACCTG   841 AAATGGAAGA AAAAAAACTT TGAACCACTG TCTGAGGGCT TGAGAATGAA CCAAAGATCC   901 AAACTCAAAA AGGGCAAATT CCAAGGAGAA ATTACATCAA GTGCCCAAGC TGGCCTAACT   961 TCAGTCTCCA CCCACTCAGT GTGGGGAAAC TCCATCGCAT AAACCCCTCC CCCCCAACCT  1021 AAAGACGACG TACTCCAAAA GCACGAGAAC TAATCGAGGT GCCTGGACGC GCCCGGTACT  1081 CCGTGAGTCA CAATGAAAGC GACGGCTGAG AACGGAAAGC CTATCTTGGG TGGGGTGGAC 1141 TCACCCCGGT CCGGCCTTCC TTCCCCTGAA GGCGGC //
Primer: pT2KXIG#1 Region: SV40pro/ori, EF-1a pro Sequence file: RDB16926_A9B7c.seq
>D06030A4_A9A7_3_SV40pro_F_V2_F01_16_ABI24.ab1     1 TACAACCTTT TTTGGAGCCT AGGCTTTTGC AAAAGCTTTG CAAAGATGGA TAAAGTTTTA    61 AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC TTGAAAGGAG TGGGAATTGG   121 CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG   181 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA   241 TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT   301 AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT   361 GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC   421 TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA   481 GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC   541 TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT   601 CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA   661 AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC   721 GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC   781 GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG   841 CCTCGCGCCG TCGTGTATCG CCCCGCCCTG GCGGCAAAGG CTGGTCTGGT CGGCACCAGT   901 TGCGTGAGCG GAAAGATGGC CGCTTCCCGG GCCCTGCTGC AGGGAGCTCA AAATGGGAGG   961 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCCACA CAAAGGAAAA GACCTTTCCC  1021 GTCCTCAGCC CGTCGCTCCA TGTGACTTCC AACGGAGTAC CGGACGTCGA TCTAGCACCT 1081 TCGATTAGTT CTCCGTTGCC TTTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl