RDB16924 - human TICAM-2 expression vector

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human TICAM-2 expression vector

Expression vector of human TICAM-2.

Catalog number	RDB16924
Resource name	human TICAM-2 expression vector
Clone info.	Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 2 (TICAM-2).
Comment	The full-length human TICAM-2 cDNA was obtained from MRC-5 cDNA by RT-PCR. Expession was confirmed by the depositor.
Vector backbone	pEFBOS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human TICAM2 cDNA
Depositor\|Developer	Seya, Tsukasa \|

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human TICAM2

External Database
human TICAM2

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16924	human TICAM-2 expression vector	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Oshiumi, H., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16924_A9B7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF-1a pro, insert 5' Sequence file: RDB16924_A9B7a.seq
>D06030A2_A9A7_1_EF1a-C_F_E01_13_ABI24.ab1 1 TATTGGATTT GGCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT 61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGAT CCCTCGACCT 121 CGAGCCACCA TGGGTATCGG GAAGTCTAAA ATAAATTCCT GCCCTCTTTC TCTCTCTTGG 181 GGTAAAAGGC ACAGTGTGGA TACAAGTCCA GGATATCATG AGTCAGATTC CAAGAAGTCT 241 GAAGATCTAT CCTTGTGTAA TGTTGCTGAG CACAGCAATA CAACAGAGGG GCCAACAGGA 301 AAGCAGGAGG GAGCTCAGAG CGTGGAAGAG ATGTTTGAAG AAGAAGCTGA AGAAGAGGTG 361 TTCCTCAAAT TTGTGATATT GCATGCAGAA GATGACACAG ATGAAGCCCT CAGAGTCCAG 421 AATCTGCTAC AAGATGACTT TGGTATCAAA CCCGGAATAA TCTTTGCTGA GATGCCATGT 481 GGCAGACAGC ATTTACAGAA TTTAGATGAT GCTGTAAATG GGTCTGCATG GACAATCTTA 541 TTACTGACTG AAAACTTTTT AAGAGATACT TGGTGTAATT TCCAGTTCTA TACGTCCCTA 601 ATGAACTCCG TTAACAGGCA GCATAAATAC AACTCTGTTA TACCCATGCG GCCCCTGAAC 661 AATCCCCTTC CCCGAGAAAG GACTCCCTTT GCCCTCCAAA CCATCAATGC CTTAGAGGAA 721 GAAAGTCGTG GATTTCCTAC ACAAGTAGAA AGAATTTTTC AGGAGTCTGT GTATAAGACA 781 CAACAAACTA TATGGAAAGA GACAAGAAAT ATGGTACAAA GACAATTTAT TGCCTGAGAT 841 GAAACATATA ACCTGCAGCC CCTGAATTCC TGCAGCCCGG GGGGATCCAC TAGTTCTAGA 901 GCGGCCGCGA CTCTAGAGTG AGGGTCCCCA CCCTGGGACC CTTGAGAGTA TCACGTCTCC 961 CACGTGGGAG ACAAGAAAAT CCCTGTTTAA ATATTTAAAC AGCAAGTGTT CCCCATCTGG 1021 GTCCCTTTGC ACCCCTCACT CCTGGTCCTC AGCCGAACTG CACAGTGGAC CCATGCCAAT 1081 CCCCCTTGAC CTGTTGA //
Primer: hGCSF_R Region: hGCSF 3'LTR + poly(A), insert 3' Sequence file: RDB16924_A9B7b.seq
>D06030A2_A9A7_1_hGCSF_R_F01_16_ABI24.ab1 1 GGGGGGACTG CATCTCTCAG GGTCCAGGTG GGGACCCTCA CTCTAGAGTC GCGGCCGCTC 61 TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCAGGGGC TGCAGGTTAT ATGTTTCATC 121 TCAGGCAATA AATTGTCTTT GTACCATATT TCTTGTCTCT TTCCATATAG TTTGTTGTGT 181 CTTATACACA GACTCCTGAA AAATTCTTTC TACTTGTGTA GGAAATCCAC GACTTTCTTC 241 CTCTAAGGCA TTGATGGTTT GGAGGGCAAA GGGAGTCCTT TCTCGGGGAA GGGGATTGTT 301 CAGGGGCCGC ATGGGTATAA CAGAGTTGTA TTTATGCTGC CTGTTAACGG AGTTCATTAG 361 GGACGTATAG AACTGGAAAT TACACCAAGT ATCTCTTAAA AAGTTTTCAG TCAGTAATAA 421 GATTGTCCAT GCAGACCCAT TTACAGCATC ATCTAAATTC TGTAAATGCT GTCTGCCACA 481 TGGCATCTCA GCAAAGATTA TTCCGGGTTT GATACCAAAG TCATCTTGTA GCAGATTCTG 541 GACTCTGAGG GCTTCATCTG TGTCATCTTC TGCATGCAAT ATCACAAATT TGAGGAACAC 601 CTCTTCTTCA GCTTCTTCTT CAAACATCTC TTCCACGCTC TGAGCTCCCT CCTGCTTTCC 661 TGTTGGCCCC TCTGTTGTAT TGCTGTGCTC AGCAACATTA CACAAGGATA GATCTTCAGA 721 CTTCTTGGAA TCTGACTCAT GATATCCTGG ACTTGTATCC ACACTGTGCC TTTTACCCCA 781 AGAGAGAGAA AGAGGGCAGG AATTTATTTT AGACTTCCCG ATACCCATGG TGGCTCGAGG 841 TCGAGGGATC TCTAGAGAAT TCCTCACGAC ACCTGAAATG GAAGAAAAAA ACTTTGAACC 901 ACTGTCTGAG GCTTGAGAAT GAACCAAGAT CCAAACTCAA AAAGGGCAAA TTTCCAAAGG 961 AGAATTTACA TCAAGGTGCC CAAGCTGGGC CTAACTTTCA GTCTCCACCC ACTTCAGTGT 1021 GGGGGAAGCT CCATTCGCAT TAAAACCCTT CCCCCCCAAC CTAGACGACG GTAACTTCGA 1081 AAGGCACGAG AAACCTAAAA TTCCG //
Primer: pT2KXIG#1 Region: SV40pro/ori, EF-1a pro Sequence file: RDB16924_A9B7c.seq
>D06030A2_A9A7_1_pT2KXIG#1_F03_18_ABI24.ab1 1 AAAAAATTCC CTTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 61 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 121 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 181 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 241 CTTTGCAAAG ATGGATAAAG TTTTAAACAG AGAGGAATCT TTGCAGCTAA TGGACCTTCT 301 AGGTCTTGAA AGGAGTGGGA ATTGGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC 361 CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT 421 GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG 481 GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG 541 CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC TTTACGGGTT 601 ATGGCCCTTG CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT CTTGATCCCG 661 AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC 721 CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC 781 ACCTTCGCGC CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAATT TTTGATGACC 841 TGCTGCGACG CTTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCTGCACA 901 CTGGGTATTT CGGTTTTTTG GGGCCGCGGG CGGCGACGGG GGCCGTGCGT CCCAAGCGCA 961 CATGTTTCGG CGAGGCGGGG TCTGCGGAGC GGCGGCCCAC CGAGGAATCG GAACGGGGGT 1021 AGGTCTTCAA GGCTGACCGG ACCTGCTTCC TGGGTGCCTG GACTTCGCGT CGATTCGATG 1081 TTATCGGCCC CCGGCCCCTT G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Oshiumi, H., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl