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pEGFP-C1-Rab35-S22N

Expression vector of mouse Rab35 constitutive negative mutant, S22N, fused with EGFP at N-terminus.

Catalog number RDB16893
Resource name pEGFP-C1-Rab35-S22N
Clone info. Expression vector of mouse Rab35 constitutive negative mutant, S22N, fused with EGFP at N-terminus.
Expression was confirmed by the depositor wiht fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab35 cDNA
Reference of nucleotide sequence AB232631 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab35 (NCBI Gene 77407) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB16893 pEGFP-C1-Rab35-S22N Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829.

2019.07.17

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