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pEGFP-C1-Rab32-T37N (#RDB16890)

Expression vector of mouse Rab32 constitutive negative mutant, T37N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab32 constitutive negative mutant, T37N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232627 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB16890zzt01.seq compiled by us

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16890 pEGFP-C1-Rab32-T37N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab32-T37N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16890).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16890_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert(T37N)
Sequence file: RDB16890_A9A8a.seq check
>D06060H1_A9A8_3_EGFP-C2_E12_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNTC NCNTGGTCCT GCTGGNNTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCGGGCG AGGGACTAGG
  121 GCAACAGGGC GCGTCGGCCA CCGCGGCGCC GGAGACCCGA GAGCACCTCT TTAAGGTGCT
  181 GGTCATCGGC GAGCTCGGCG TGGGTAAGAA CAGCATCATC AAGCGCTACG TGCACCAGCT
  241 CTTCTCCCAG CACTACCGCG CCACCATTGG CGTGGACTTC GCCCTCAAAG TTCTCAACTG
  301 GGACAGCAGG ACGCTCGTGC GCCTGCAGCT GTGGGATATC GCGGGACAGG AACGGTTTGG
  361 CAACATGACT CGAGTATACT ATAAGGAAGC TCTTGGAGCA TTTGTAGTCT TTGATATATC
  421 GAGAAGTTCT ACATTTGATG CAGTCCTAAA ATGGAAAAAC GATCTGGATA GTAAAGTTCA
  481 TCTTCCCAAC GGCAGCCCCA TTCCTGCTGT CCTCCTAGCT AACAAATGTG ACCAGAAGAA
  541 GGACAACAGC CAGAGTCCTT CCCAGATGGA CCAGTTTTGT AAAGACCATG GCTTCACTGG
  601 ATGGTTTGAA ACCTCTGCCA AGGATAATAT AAACATCGAC GAGGCCACCC GCTTCCTAGT
  661 GGAAAACATG CTTGCAAACC AGCAAAGTTT TCCTAGTGAA GAAATCGACC TGGACAGAAT
  721 TAAACTGGTA GAGGAGCCCC CCACAACAAA GCCCAGGTCC CAGTGCTGCT GANGTGTCTC
  781 TGTGAATCAC TAGTGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGANC CACCGGNTCT
  841 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG NTTTACTTGC TTTAAAAAAN
  901 CTCCCACACC NCCCCNNGAA NCTGAAANNT AAAATGAANG NNNTTGNTGN TGNTNACNTG
  961 NTTANNGNAN CTTANNNNGN NNANNAATAA ANNNANANCA TNNNNAANTN NNNNNNNANN
1021 NNTTTTTTNN NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert(T37N)
Sequence file: RDB16890_A9A8b.seq check
>D06060H1_A9A8_3_SV40polyA-R2_F12_18_ABI24.ab1
    1 NNNNNNNNNC NGNNNNNNNN NNGTTAACNN NNACAATTGC ATTCNTTTTA TGTTTCNGGT
   61 TCNGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG
  181 AATTCACTAG TGATTCACAG AGACACATCA GCAGCACTGG GACCTGGGCT TTGTTGTGGG
  241 GGGCTCCTCT ACCAGTTTAA TTCTGTCCAG GTCGATTTCT TCACTAGGAA AACTTTGCTG
  301 GTTTGCAAGC ATGTTTTCCA CTAGGAAGCG GGTGGCCTCG TCGATGTTTA TATTATCCTT
  361 GGCAGAGGTT TCAAACCATC CAGTGAAGCC ATGGTCTTTA CAAAACTGGT CCATCTGGGA
  421 AGGACTCTGG CTGTTGTCCT TCTTCTGGTC ACATTTGTTA GCTAGGAGGA CAGCAGGAAT
  481 GGGGCTGCCG TTGGGAAGAT GAACTTTACT ATCCAGATCG TTTTTCCATT TTAGGACTGC
  541 ATCAAATGTA GAACTTCTCG ATATATCAAA GACTACAAAT GCTCCAAGAG CTTCCTTATA
  601 GTATACTCGA GTCATGTTGC CAAACCGTTC CTGTCCCGCG ATATCCCACA GCTGCAGGCG
  661 CACGAGCGTC CTGCTGTCCC AGTTGAGAAC TTTGAGGGCG AAGTCCACGC CAATGGTGGC
  721 GCGGTAGTGC TGGGAGAAGA GCTGGTGCAC GTAGCGCTTG ATGATGCTGT TCTTACCCAC
  781 GCCGAGCTCG CCGATGACCA GCACCTTAAA GANGTGCTCT CGGGTCTCCG GCGCCGCGGN
  841 GGCCGACGCG CCCTGTTGNC CTANTCCNTC GCCCGCCATG GATCTGANTN CNGANTTGTA
  901 CAGCTCGNNC ATGCCNAGAG TGATCCCNGN GGNNGNCACG AACTCCANCA NGANNNTGNG
  961 ATNNCNCTTC NCNNNGGGGN TCNTTGCNCN NNNNGNANTG GGNGNNCNNN TANNNGNNNG
1021 NNNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB16890_A9A8c.seq check
>D06060H1_A9A8_3_CMV_Forward_G12_21_ABI24.ab1
    1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC
  721 ACCCAGTCCG CCCTGANCAA AGANCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGNCNANC TGTACNAGTC CGGANTCNNA
  841 TNNNTGGCGG GCGANGGNNT AGGGCAACAG GGNGCGNCGG NCNNCGCGGC GCCNGANNNC
  901 NGANANCACN NCTTTNANNN GCTGNNCNTC GNNNANCNNN GNNNNNNNNN ANNAGCATCN
961 TCNNGCGCNN CNNGCNNNNN CNNNNNNNCN NNNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16890_A9A8d.seq check
>D06060H1_A9A8_3_SV40pro_F_V2_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN GCTTTTNNNN NNNCGATCAA GANACAGGNT GAGGNTCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACNAG TTCTTCTGAN CGGGNNTCTG GGGNTCGAAA TGACCGACCN AGCGACGCCC
  901 AACCTGNNNN NNCGAGATTT CGANTCCACC GCCNCCNTCT ATGAAANGNT GGGCTTCNGA
  961 ATCGTTTTNN NGGNNGNCNG GNNNNNNNAN NNNNCNNNNN NGGNNNNNNN NGNNNGNNNN
1021 NNNNNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'(T37N)
Sequence file: RDB16890_A9A8e.seq check
>16890_16890_B1JdF_mVenus-M_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNNNNNNNT NNAGATCCGC CACAACATCG AGGACGGCAG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
  241 GCTGTACAAG TCCGGACTCA GATCCATGGC GGGCGAGGGA CTAGGGCAAC AGGGCGCGTC
  301 GGCCACCGCG GCGCCGGAGA CCCGAGAGCA CCTCTTTAAG GTGCTGGTCA TCGGCGAGCT
  361 CGGCGTGGGT AAGAACAGCA TCATCAAGCG CTACGTGCAC CAGCTCTTCT CCCAGCACTA
  421 CCGCGCCACC ATTGGCGTGG ACTTCGCCCT CAAAGTTCTC AACTGGGACA GCAGGACGCT
  481 CGTGCGCCTG CAGCTGTGGG ATATCGCGGG ACAGGAACGG TTTGGCAACA TGACTCGAGT
  541 ATACTATAAG GAAGCTCTTG GAGCATTTGT AGTCTTTGAT ATATCGAGAA GTTCTACATT
  601 TGATGCAGTC CTAAAATGGA AAAACGATCT GGATAGTNAA GTTCATCTTC CCNACNGCAG
  661 CCCCNTTCCT GCNGNNCTCC TANCTNACAN NGTGANNNGN ANNNNNNNAN NNNNNNANNN
721 NNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]