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pEGFP-C1-Rab27A-Q78L (#RDB16889)

Expression vector of mouse Rab27A constitutive active mutant, Q78L, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab27A constitutive active mutant, Q78L, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab27a cDNA
Reference of insert sequence AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16889 pEGFP-C1-Rab27A-Q78L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab27A-Q78L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16889).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16889_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5' (Q78L)
Sequence file: RDB16889_A9A8a.seq check
>D06060G9_A9A8_1_EGFP-C2_A12_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGN TCNCNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGATC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGTCGGA TGGAGATTAC
  121 GATTACCTCA TCAAGTTCTT GGCCTTGGGA GACTCTGGGG TAGGGAAGAC CAGTGTACTC
  181 TACCAGTACA CTGATGGCAA GTTCAACTCC AAATTCATCA CCACAGTGGG CATTGATTTC
  241 AGGGAAAAGA GAGTGGTGTA CAGAGCCAAT GGGCCAGATG GAGCTGTGGG CCGAGGCCAG
  301 AGAATCCACC TGCAGTTATG GGACACGGCG GGGCTGGAGA GGTTTCGGAG CTTAACCACT
  361 GCATTCTTCA GGGACGCTAT GGGTTTCCTG CTTCTGTTCG ACCTGACAAA TGAGCAAAGT
  421 TTCCTCAATG TCCGAAACTG GATAAGCCAG CTACAGATGC ACGCGTACTG TGAAAACCCA
  481 GATATAGTGC TGTGTGGAAA TAAGAGTGAC CTGGAAGACC AGAGGGCAGT GAAAGAGGAG
  541 GAGGCCCGGG AACTTGCCGA GAAGTACGGA ATCCCCTATT TTGAAACCAG TGCTGCCAAC
  601 GGGACAAACA TAAGCCACGC GATTGAGATG CTCCTGGANC TGATCATGAA GCGGATGGAG
  661 CGGTGTGTGG ACAAGTCCTG GATTCCGGAN GGAGTGGTAC GGTCCAACGG CCNTACCTCT
  721 GCGGANCAGC TAAGTGANGA GAANGAGAAN GGGTTGTGTG GCTGTTGAAA TCACTAGTGA
  781 ANTCTGCAGT CGANGGTACC GCGGGCCCNG GNNNCNNCNG ATCTNGATAA CNGATCNTAA
  841 TCAGCCNTAC CANATTTGTA NANGNTTTAC TNGCTTTNAA AANNNTCCNN CACNNCCCNN
901 NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16889_A9A8b.seq check
>D06060G9_A9A8_1_SV40polyA-R2_B12_06_ABI24.ab1
    1 NNNNNNNNCN NNNNNNNNNA GTTAACNANN ACNATTGCAT TCATTTTATG TTTCAGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTCAACAG CCACACAACC CCTTCTCCTT CTCCTCACTT AGCTGATCCG
  241 CAGAGGTATG GCCGTTGGAC CGTACCACTC CCTCCGGAAT CCAGGACTTG TCCACACACC
  301 GCTCCATCCG CTTCATGATC AGGTCCAGGA GCATCTCAAT CGCGTGGCTT ATGTTTGTCC
  361 CGTTGGCAGC ACTGGTTTCA AAATAGGGGA TTCCGTACTT CTCGGCAAGT TCCCGGGCCT
  421 CCTCCTCTTT CACTGCCCTC TGGTCTTCCA GGTCACTCTT ATTTCCACAC AGCACTATAT
  481 CTGGGTTTTC ACAGTACGCG TGCATCTGTA GCTGGCTTAT CCAGTTTCGG ACATTGAGGA
  541 AACTTTGCTC ATTTGTCAGG TCGAACAGAA GCAGGAAACC CATAGCGTCC CTGAAGAATG
  601 CAGTGGTTAA GCTCCGAAAC CTCTCCAGCC CCGCCGTGTC CCATAACTGC AGGTGGATTC
  661 TCTGGCCTCG GCCCACAGCT CCATCTGGCC CATTGGCTCT GTACACCACT CTCTTTTCCC
  721 TGAAATCAAT GCCCACTGTG GTGATGAATT TGGAGTTGAA CTTGCCATCA GTGTACTGGT
  781 AGAGTACACT GGTCTTCCCT ACCCCAGANT CTCCCAANGC CAAGAACTTG ATGANGTAAT
  841 CGNAATCTCC ATCCGANATG GNTCTGANNN CGGANNTGNN CAGCTCGTCC NNGNCNANAN
  901 TGANNCCGGN GGCGGNCNCG ANNNNCNNCN NNANCNTGTG ATCNCNCNNN NCNNNGGGNN
961 CNTTGNNNNN GNCNNNNNNN NNGNNCNNNT ANNNNNNNNN N
//
Primer: CMV_Forward
Sequence file: RDB16889_A9A8c.seq check
>D06060G9_A9A8_1_CMV_Forward_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  721 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 GTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGNCGAN CTGTACAAGT CCGGANTCAG
  841 ATCCATGTCG GANGGAGATT ACGATTANCT CATCAAGTTC TNNGCCTTNN NAGANTCNNG
  901 GGNANGNNAN ANCANNNNAN NCTACNANNA CNNNGNTNGN NNGNTCNNCT CNNAATTCNT
961 CANNNNANNG GNNNNTGNTT TCNNNNNANN NNNNNNNN
//
Primer: SV40pro_F_V2
Sequence file: RDB16889_A9A8d.seq check
>D06060G9_A9A8_1_SV40pro_F_V2_D12_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN NGGCTTTTGC NNAGNNCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCANGACATA GCGTTGGCTA CNNGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TNCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GANTTCTTCT GANCGGGANT CTGGGGTTCG AAATGNNNNA NCNNNCGANG
  901 CCCNNNCTGN CNTCACNANA TTNCNATTNC NCNGCCGNCN TCTATGNAAN NNNNGGGCTT
961 NNNNNNCNTT TTNCNGGGNN NNNNGNTNNN NGANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
reference Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]