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pEGFP-C1-Rab27A-T23N (#RDB16888)

Expression vector of mouse Rab27A constitutive negative mutant, T23N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab27A constitutive negative mutant, T23N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab27a cDNA
Reference of insert sequence AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16888 pEGFP-C1-Rab27A-T23N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab27A-T23N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16888).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16888_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5' (T23N)
Sequence file: RDB16888_A9A8a.seq check
>D06060G8_A9A8_2_EGFP-C2_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNCN CNTGGTCCTG CTGGANTTCG TGANCGCCGC CGGGATCACT
   61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGTCGGATGG AGATTACGAT
  121 TACCTCATCA AGTTCTTGGC CTTGGGAGAC TCTGGGGTAG GGAAGAACAG TGTACTCTAC
  181 CAGTACACTG ATGGCAAGTT CAACTCCAAA TTCATCACCA CAGTGGGCAT TGATTTCAGG
  241 GAAAAGAGAG TGGTGTACAG AGCCAATGGG CCAGATGGAG CTGTGGGCCG AGGCCAGAGA
  301 ATCCACCTGC AGTTATGGGA CACGGCGGGG CAGGAGAGGT TTCGGAGCTT AACCACTGCA
  361 TTCTTCAGGG ACGCTATGGG TTTCCTGCTT CTGTTCGACC TGACAAATGA GCAAAGTTTC
  421 CTCAATGTCC GAAACTGGAT AAGCCAGCTA CAGATGCACG CGTACTGTGA AAACCCAGAT
  481 ATAGTGCTGT GTGGAAATAA GAGTGACCTG GAAGACCAGA GGGCAGTGAA AGAGGAGGAG
  541 GCCCGGGAAC TTGCCGAGAA GTACGGAATC CCCTATTTTG AAACCAGTGC TGCCAACGGG
  601 ACAAACATAA GCCACGCGAT TGAGATGCTC CTGGACCTGA TCATGAAGCG GATGGAGCGG
  661 TGTGTGGACA AGTCCTGGAT TCCGGAGGGA GTGGTACGGT CCAACGGCCA TACCTCTGCG
  721 GATCAGCTAA GTGAGGAGAA GGAGAAGGGG TTGTGTGGCT GTTGAAATCA CTAGTGAATT
  781 CTGCAGTCGA CGGTACCGCG GGCCCGGGAN CCACCGGNNC TAGATAACTG ATCATAATCA
  841 GCCNTACCAC ATTTGTAGAG GNTTTACTTG CTTTNAAAAA CCTCCCACAC NTCCCCCNGA
  901 ANCTGAAACA TNAAATGANT GNNNNTGTTG TTGTTNACTT GNTTANTGNA GCTTNNANNG
  961 GNTANNANTA AAGCNNNANC ATNNNNAATT TCNNNAATAA NNNNTTTTTT TNNNNNNNNN
1021 N
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16888_A9A8b.seq check
>D06060G8_A9A8_2_SV40polyA-R2_F11_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAGTT AANNACNANN ATTGCATTCN TTTTATGTTT CNGGTTCAGG
   61 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  121 ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC TGCAGAATTC
  181 ACTAGTGATT TCAACAGCCA CACAACCCCT TCTCCTTCTC CTCACTTAGC TGATCCGCAG
  241 AGGTATGGCC GTTGGACCGT ACCACTCCCT CCGGAATCCA GGACTTGTCC ACACACCGCT
  301 CCATCCGCTT CATGATCAGG TCCAGGAGCA TCTCAATCGC GTGGCTTATG TTTGTCCCGT
  361 TGGCAGCACT GGTTTCAAAA TAGGGGATTC CGTACTTCTC GGCAAGTTCC CGGGCCTCCT
  421 CCTCTTTCAC TGCCCTCTGG TCTTCCAGGT CACTCTTATT TCCACACAGC ACTATATCTG
  481 GGTTTTCACA GTACGCGTGC ATCTGTAGCT GGCTTATCCA GTTTCGGACA TTGAGGAAAC
  541 TTTGCTCATT TGTCAGGTCG AACAGAAGCA GGAAACCCAT AGCGTCCCTG AAGAATGCAG
  601 TGGTTAAGCT CCGAAACCTC TCCTGCCCCG CCGTGTCCCA TAACTGCAGG TGGANTCTCT
  661 GGCCTCGGCC CACAGCTCCA TCTGGCCCAT TGGCTCTGTA CACCACTCTC TTTTCCCTGA
  721 AATCAATGCC CACTGTGGTG ATGAATTTGG AGTTGAACTT GCCATCAGTG TACTGGTAGA
  781 GTACACTGTT CTTCCCTACN NCAGANTCTC CCNANGCCAA GANNTTGATG ANGTAATCGT
  841 AATCTCCATC CGANATGGAT CTGANNCCNG ANTTGTACAG CTCGNNNNTG NCGANANTGA
  901 TNCCNGCGGC NGNCNCGANC TCCAGCNNNA NNNTGTGNTC GCNCTTNNCG NTGGGGTCTT
  961 NGCNCNGGNC GGNNNNNNNN NTCNNNNANN NGNNNGNNGG NNANCANCNN GGGGNCNNNN
1021 NNNNNN
//
Primer: CMV_Forward
Sequence file: RDB16888_A9A8c.seq check
>D06060G8_A9A8_2_CMV_Forward_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNTTGNNN CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN
  781 TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGNNCGANC TGTACAAGTC CGGANTCAGA
  841 TCCNTGTCGG ANGGAGATTA CGATTACCTC ATCAAGTTCT NGGNCTNNGG ANANTCTGGG
  901 GNANGGAAGA ANAGTGTACT CTANNANNAC NCNGATGGNN AGTTCNNCNN CNAATTCNTC
961 ACNNCNNNGG GNNNNNNNTN CNNGNNAANN ANNNNNNNNN N
//
Primer: SV40pro_F_V2
Sequence file: RDB16888_A9A8d.seq check
>D06060G8_A9A8_2_SV40pro_F_V2_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNAAGATCGA TCNAGAGACA GGANGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGNCCG GCTGGGTGTG GCGGANCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAANA GCTTGGCGGC GAATGGGCTG
  781 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCANCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGANCGGGNN TCTGGGGTTC GAAATGNCCG ACNNANCGAN
  901 GCCCNACCNG NCNNNNCNAG NNTTCGANTC CNNNGNNNCC NTCNNTGNAA GGNNGGGCTT
961 NNGNNNNNTT TTNCNNGNNN NNCNGNNNNN NNGANNCNTN NNNNNNNNGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
reference Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080. [link to RRC of NBRP]