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pEGFP-C1-Rab9A-S21N (#RDB16879)

Expression vector of mouse Rab9A constitutive negative mutant, S21N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab9A constitutive negative mutant, S21N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab9a cDNA
Reference of insert sequence AB232602 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB16879zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16879 pEGFP-C1-Rab9A-S21N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab9A-S21N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16879).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16879_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert(S21N)
Sequence file: RDB16879_A9A8a.seq check
>D06060F8_A9A8_3_EGFP-C2_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNCNNGNNCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
   61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGCAGGAAA ATCGTCTCTT
  121 TTTAAAATAA TTCTTCTTGG AGATGGTGGA GTTGGCAAGA ATTCTCTTAT GAACAGATAT
  181 GTAACCAATA AATTTGATTC CCAGCTCTTC CACACAATAG GTGTGGAATT TTTAAATAAA
  241 GATCTGGAGG TGGACGGACA TTTTGTTACC ATGCAGATTT GGGACACAGC TGGCCAAGAA
  301 CGCTTCCGAA GCCTGAGGAC GCCATTTTAC CGGGGTTCTG ACTGTTGCCT GCTTACATTT
  361 AGTGTCGATG ATTCACAGAG CTTCCAGAAT TTGAGCAACT GGAAGAAAGA GTTCATATAT
  421 TACGCAGATG TGAAAGAGCC TGAAAGCTTT CCCTTTGTGA TTTTGGGCAA CAAGACTGAC
  481 ATAAAAGAAC GGCAGGTGTC TACAGAAGAA GCCCAAGCCT GGTGCAAGGA CAACGGCGAC
  541 TATCCTTACT TTGAAACAAG TGCAAAAGAT TCCACCAATG TTGCTGCTGC CTTTGAGGAG
  601 GCAGTTCGAA GAATTCTGGC CACGGAAGAT AGGTCAGAAC ACCTGATTCA GACAGACACA
  661 GTCAATCTGC ACCGAAAGCC CAAGCCAAAC TCATCTTGCT GTTGAAATCA CTAGTGAATT
  721 CTGCAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC TAGATAACTG ATCATAATCA
  781 GCCATACNAC ATTTGTAGAG GNTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCNNCTGA
  841 ACCTGAAACA TAAAATGAAT GCAATTGTTG NTGTTAACTT GNTTANTGCA GCTTATAATG
  901 NNTACNAATA AAGCAATAGC ATCNCAAATT TCACAAATNA AGNATTTTTT TTCNNNGNAT
  961 TCNANNNGTG GNTNGNCCNA ACNNNTCNNN GNATNNNNNC NNNNNAANTN NNNAGCNNNN
1021 NNNNTTNNNN NAANNNNNNN NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40 pA,insert
Sequence file: RDB16879_A9A8b.seq check
>D06060F8_A9A8_3_SV40polyA-R2_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCACTA GTGATTTCAA CAGCAAGATG AGTTTGGCTT GGGCTTTCGG TGCAGATTGA
  241 CTGTGTCTGT CTGAATCAGG TGTTCTGACC TATCTTCCGT GGCCAGAATT CTTCGAACTG
  301 CCTCCTCAAA GGCAGCAGCA ACATTGGTGG AATCTTTTGC ACTTGTTTCA AAGTAAGGAT
  361 AGTCGCCGTT GTCCTTGCAC CAGGCTTGGG CTTCTTCTGT AGACACCTGC CGTTCTTTTA
  421 TGTCAGTCTT GTTGCCCAAA ATCACAAAGG GAAAGCTTTC AGGCTCTTTC ACATCTGCGT
  481 AATATATGAA CTCTTTCTTC CAGTTGCTCA AATTCTGGAA GCTCTGTGAA TCATCGACAC
  541 TAAATGTAAG CAGGCAACAG TCAGAACCCC GGTAAAATGG CGTCCTCAGG CTTCGGAAGC
  601 GTTCTTGGCC AGCTGTGTCC CAAATCTGCA TGGTAACAAA ATGTCCGTCC ACCTCCAGAT
  661 CTTTATTTAA AAATTCCACA CCTATTGTGT GGAAGAGCTG GGAATCAAAT TTATTGNTTA
  721 CATATCTGTT CATAAGAGAA TTCTTGCNAA CTCCACCATC TCCNAGAAGA ATTATTTTAA
  781 AAAGAGACGA NTTTCCTGCC ATGGNTCTGA NTCCGGANTT GTACAGCTCG TCCATGCCGA
  841 GAGTGATNCC GGCGGCGGNC ACGAACTCCA GCNNGNNCAT GTGATCGCGC TTCTCGTTGG
  901 GGNCTTTGCT CNNNNGGANT GGGNGNTCNN NTANTGGTTG TCGGGCANCA NCACGGGGNN
961 GNCGCNNANG GGGGTGNTCN GNNNGNANNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB16879_A9A8c.seq check
>D06060F8_A9A8_3_CMV_Forward_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGNGCTGCT GCCCGACAAC CACTACCTGA
  721 GCACCCAGTC CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 ANTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGNCGA NCTGTACNAG TCCGGANTCA
  841 GATCCATGGC ANGAAAATCG TCTCTTTTTA AAATAATTCT TCTTGGANAT GGTGGAGTNG
  901 GNAAGAANTC TCTTATGAAC AGATATGTAA NCNATAAATT TGANTCCNNG CTCTTNCNCA
  961 CNNNNNNNGN GNAANTTTTA AATNANNNNC NGNNNNNGGN NGGNNNTTTT GNNNNNNNGC
1021 ANANTTGGNN NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16879_A9A8d.seq check
>D06060F8_A9A8_3_SV40pro_F_V2_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGCTTTTG NAAGANCGAT CAAGAGANNG GNTGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCNTCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GNATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGNNT CTGGGGNTCG AAATGANCGA NCAAGCGACG
  901 CCCNACCNGC CNTCACGAGA TTTCGATTCC NCCGCCGCCT TCTATGAAAN GNNTGGNNTN
961 CNNAATCGTT TTNNNNNNNN NN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'(S21N)
Sequence file: RDB16879_A9A8e.seq check
>16879_16879_B1JdF_mVenus-M_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNGGNNTNNG GTGNACTTNN AGANCCGCCN CAACATCGAG GACGGCAGCG
   61 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
  121 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
  181 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
  241 TGTACAAGTC CGGACTCAGA TCCATGGCAG GAAAATCGTC TCTTTTTAAA ATAATTCTTC
  301 TTGGAGATGG TGGAGTTGGC AAGAATTCTC TTATGAACAG ATATGTAACC AATAAATTTG
  361 ATTCCCAGCT CTTCCACACA ATAGGTGTGG AATTTTTAAA TAAAGATCTG GAGGTGGACG
  421 GACATTTTGT TACCATGCAG ATTTGGGACA CAGCTGGCCA AGAACGCTTC CGAAGCCTGA
  481 GGACGCCATT TTACCGGGGT TCTGACTGTT GCCTGCTTAC ATTTAGTGTC GATGATTCAC
  541 AGAGCTTCCA GAATTTGAGC AACTGGAAGA AAGAGTTCAT ATATTACGCA GATGTGAAAG
  601 AGCCTGAAAG CTTTCCCTTT GTGANTTTGG GCAACAAGAN TGACATAAAA GAACGGCANG
  661 TGTCTACNGA ANAANCCCNA GCCTNNNCNN NGANANGGNN ACTATNNNNA NTTTGNANCN
721 NNGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]