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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab9A-S21N

Expression vector of mouse Rab9A constitutive negative mutant, S21N, fused with EGFP at N-terminus.

Catalog number RDB16879
Resource name pEGFP-C1-Rab9A-S21N
Clone info. Expression vector of mouse Rab9A constitutive negative mutant, S21N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab9a cDNA
Reference of insert sequence AB232602 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab9a

          Reference sequence
            
           
          Sequence (full) RDB16879zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB16879 pEGFP-C1-Rab9A-S21N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16879_A9A8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3',insert(S21N)
          Sequence file: RDB16879_A9A8a.seq check
          >D06060F8_A9A8_3_EGFP-C2_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNCNNGNNCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
             61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGCAGGAAA ATCGTCTCTT
            121 TTTAAAATAA TTCTTCTTGG AGATGGTGGA GTTGGCAAGA ATTCTCTTAT GAACAGATAT
            181 GTAACCAATA AATTTGATTC CCAGCTCTTC CACACAATAG GTGTGGAATT TTTAAATAAA
            241 GATCTGGAGG TGGACGGACA TTTTGTTACC ATGCAGATTT GGGACACAGC TGGCCAAGAA
            301 CGCTTCCGAA GCCTGAGGAC GCCATTTTAC CGGGGTTCTG ACTGTTGCCT GCTTACATTT
            361 AGTGTCGATG ATTCACAGAG CTTCCAGAAT TTGAGCAACT GGAAGAAAGA GTTCATATAT
            421 TACGCAGATG TGAAAGAGCC TGAAAGCTTT CCCTTTGTGA TTTTGGGCAA CAAGACTGAC
            481 ATAAAAGAAC GGCAGGTGTC TACAGAAGAA GCCCAAGCCT GGTGCAAGGA CAACGGCGAC
            541 TATCCTTACT TTGAAACAAG TGCAAAAGAT TCCACCAATG TTGCTGCTGC CTTTGAGGAG
            601 GCAGTTCGAA GAATTCTGGC CACGGAAGAT AGGTCAGAAC ACCTGATTCA GACAGACACA
            661 GTCAATCTGC ACCGAAAGCC CAAGCCAAAC TCATCTTGCT GTTGAAATCA CTAGTGAATT
            721 CTGCAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC TAGATAACTG ATCATAATCA
            781 GCCATACNAC ATTTGTAGAG GNTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCNNCTGA
            841 ACCTGAAACA TAAAATGAAT GCAATTGTTG NTGTTAACTT GNTTANTGCA GCTTATAATG
            901 NNTACNAATA AAGCAATAGC ATCNCAAATT TCACAAATNA AGNATTTTTT TTCNNNGNAT
            961 TCNANNNGTG GNTNGNCCNA ACNNNTCNNN GNATNNNNNC NNNNNAANTN NNNAGCNNNN
          1021 NNNNTTNNNN NAANNNNNNN NNN
          //
          Primer: SV40polyA_R2
          Region: SV40 pA,insert
          Sequence file: RDB16879_A9A8b.seq check
          >D06060F8_A9A8_3_SV40polyA-R2_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
            181 GAATTCACTA GTGATTTCAA CAGCAAGATG AGTTTGGCTT GGGCTTTCGG TGCAGATTGA
            241 CTGTGTCTGT CTGAATCAGG TGTTCTGACC TATCTTCCGT GGCCAGAATT CTTCGAACTG
            301 CCTCCTCAAA GGCAGCAGCA ACATTGGTGG AATCTTTTGC ACTTGTTTCA AAGTAAGGAT
            361 AGTCGCCGTT GTCCTTGCAC CAGGCTTGGG CTTCTTCTGT AGACACCTGC CGTTCTTTTA
            421 TGTCAGTCTT GTTGCCCAAA ATCACAAAGG GAAAGCTTTC AGGCTCTTTC ACATCTGCGT
            481 AATATATGAA CTCTTTCTTC CAGTTGCTCA AATTCTGGAA GCTCTGTGAA TCATCGACAC
            541 TAAATGTAAG CAGGCAACAG TCAGAACCCC GGTAAAATGG CGTCCTCAGG CTTCGGAAGC
            601 GTTCTTGGCC AGCTGTGTCC CAAATCTGCA TGGTAACAAA ATGTCCGTCC ACCTCCAGAT
            661 CTTTATTTAA AAATTCCACA CCTATTGTGT GGAAGAGCTG GGAATCAAAT TTATTGNTTA
            721 CATATCTGTT CATAAGAGAA TTCTTGCNAA CTCCACCATC TCCNAGAAGA ATTATTTTAA
            781 AAAGAGACGA NTTTCCTGCC ATGGNTCTGA NTCCGGANTT GTACAGCTCG TCCATGCCGA
            841 GAGTGATNCC GGCGGCGGNC ACGAACTCCA GCNNGNNCAT GTGATCGCGC TTCTCGTTGG
            901 GGNCTTTGCT CNNNNGGANT GGGNGNTCNN NTANTGGTTG TCGGGCANCA NCACGGGGNN
          961 GNCGCNNANG GGGGTGNTCN GNNNGNANNN NNNNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro,EGFP
          Sequence file: RDB16879_A9A8c.seq check
          >D06060F8_A9A8_3_CMV_Forward_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
            121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
            181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
            241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
            301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
            361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
            421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
            481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
            541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
            601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
            661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGNGCTGCT GCCCGACAAC CACTACCTGA
            721 GCACCCAGTC CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
            781 ANTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGNCGA NCTGTACNAG TCCGGANTCA
            841 GATCCATGGC ANGAAAATCG TCTCTTTTTA AAATAATTCT TCTTGGANAT GGTGGAGTNG
            901 GNAAGAANTC TCTTATGAAC AGATATGTAA NCNATAAATT TGANTCCNNG CTCTTNCNCA
            961 CNNNNNNNGN GNAANTTTTA AATNANNNNC NGNNNNNGGN NGGNNNTTTT GNNNNNNNGC
          1021 ANANTTGGNN NNNNNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR_KanR
          Sequence file: RDB16879_A9A8d.seq check
          >D06060F8_A9A8_3_SV40pro_F_V2_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGCTTTTG NAAGANCGAT CAAGAGANNG GNTGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCNTCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GNATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GAGCGGGNNT CTGGGGNTCG AAATGANCGA NCAAGCGACG
            901 CCCNACCNGC CNTCACGAGA TTTCGATTCC NCCGCCGCCT TCTATGAAAN GNNTGGNNTN
          961 CNNAATCGTT TTNNNNNNNN NN
          //
          Primer: mVenus-M_F
          Region: EGFP 3',insert 5'(S21N)
          Sequence file: RDB16879_A9A8e.seq check
          >16879_16879_B1JdF_mVenus-M_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNGGNNTNNG GTGNACTTNN AGANCCGCCN CAACATCGAG GACGGCAGCG
             61 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
            121 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
            181 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
            241 TGTACAAGTC CGGACTCAGA TCCATGGCAG GAAAATCGTC TCTTTTTAAA ATAATTCTTC
            301 TTGGAGATGG TGGAGTTGGC AAGAATTCTC TTATGAACAG ATATGTAACC AATAAATTTG
            361 ATTCCCAGCT CTTCCACACA ATAGGTGTGG AATTTTTAAA TAAAGATCTG GAGGTGGACG
            421 GACATTTTGT TACCATGCAG ATTTGGGACA CAGCTGGCCA AGAACGCTTC CGAAGCCTGA
            481 GGACGCCATT TTACCGGGGT TCTGACTGTT GCCTGCTTAC ATTTAGTGTC GATGATTCAC
            541 AGAGCTTCCA GAATTTGAGC AACTGGAAGA AAGAGTTCAT ATATTACGCA GATGTGAAAG
            601 AGCCTGAAAG CTTTCCCTTT GTGANTTTGG GCAACAAGAN TGACATAAAA GAACGGCANG
            661 TGTCTACNGA ANAANCCCNA GCCTNNNCNN NGANANGGNN ACTATNNNNA NTTTGNANCN
          721 NNGNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
          reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.08.12

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