Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab7-Q67L (#RDB16878)

Expression vector of mouse Rab7A constitutive active mutant, Q67L, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab7A constitutive active mutant, Q67L, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16878 pEGFP-C1-Rab7-Q67L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab7-Q67L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16878).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16878_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert
Sequence file: RDB16878_A9A8a.seq check
>D06060F7_A9A8_3_EGFP-C2_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNAANCGCG NNCNCNTGGT CCTGCTGGAN TTCGTGACCG CCGCCGGGAT
   61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGACCT CTAGGAAGAA
  121 AGTGTTGCTG AAGGTCATCA TCCTGGGGGA CTCTGGTGTT GGAAAGACCT CTCTCATGAA
  181 CCAGTATGTG AACAAGAAGT TCAGTAACCA GTACAAAGCC ACAATAGGAG CGGACTTTCT
  241 GACCAAGGAG GTGATGGTGG ACGACAGACT TGTTACCATG CAGATCTGGG ACACAGCCGG
  301 TCTAGAACGG TTCCAGTCTC TTGGTGTGGC CTTCTACAGA GGTGCAGATT GCTGTGTTCT
  361 GGTGTTTGAT GTGACTGCCC CCAACACTTT CAAAACCCTC GACAGCTGGA GAGACGAGTT
  421 TCTCATCCAG GCCAGCCCCC GGGATCCCGA GAACTTCCCT TTTGTTGTGT TGGGAAACAA
  481 GATTGACCTG GAAAACAGAC AAGTGGCCAC AAAGAGGGCA CAGGCTTGGT GCTACAGCAA
  541 AAACAACATT CCTTACTTCG AGACCAGTGC CAAGGAGGCC ATCAATGTGG AGCAGGCCTT
  601 CCAGACAATT GCTCGGAATG CCCTTAAACA GGAAACAGAA GTGGAACTGT ACAATGAATT
  661 CCCTGAACCC ATCAAACTGG ACAAGAATGA CCGGGCCAAG GCCTCCGCAG AAAGCTGCAG
  721 TTGTTGAAAT CACTAGTGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA
  781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
  841 AACCTCCCAC ACCTCNNCCN NAACCTGAAA CATAAAATGA ATGCNATTGT TGTTGTTAAC
  901 NTNGTTTATT GCAGCTTATA ATGNNTACAA ATAAAGCAAT ANCATCACNA ATTTCACAAA
  961 TAAAGCATTT TTTTTCNCNG CATTCNANNN GNNGNNTTGN NCNAAANNCN NCNANGNNNN
1021 NNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16878_A9A8b.seq check
>D06060F7_A9A8_3_SV40polyA-R2_F06_18_ABI24.ab1
    1 NNNNNNNNNC NNNANNNANN NGTTAANNAC NACNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCAACA ACTGCAGCTT TCTGCGGAGG CCTTGGCCCG GTCATTCTTG
  241 TCCAGTTTGA TGGGTTCAGG GAATTCATTG TACAGTTCCA CTTCTGTTTC CTGTTTAAGG
  301 GCATTCCGAG CAATTGTCTG GAAGGCCTGC TCCACATTGA TGGCCTCCTT GGCACTGGTC
  361 TCGAAGTAAG GAATGTTGTT TTTGCTGTAG CACCAAGCCT GTGCCCTCTT TGTGGCCACT
  421 TGTCTGTTTT CCAGGTCAAT CTTGTTTCCC AACACAACAA AAGGGAAGTT CTCGGGATCC
  481 CGGGGGCTGG CCTGGATGAG AAACTCGTCT CTCCAGCTGT CGAGGGTTTT GAAAGTGTTG
  541 GGGGCAGTCA CATCAAACAC CAGAACACAG CAATCTGCAC CTCTGTAGAA GGCCACACCA
  601 AGAGACTGGA ACCGTTCTAG ACCGGCTGTG TCCCAGATCT GCATGGTAAC AAGTCTGTCG
  661 TCCACCATCA CCTCCTTGGT CAGAAAGTCC GCTCCTATTG TGGCTTTGTA CTGGTTACTG
  721 AACTTCTTGT TCACATACTG GTTCATGAGA GAGGTCTTTC CAACACCAGA GTCCCCCAGG
  781 ATGATGANCT TCAGCAACAC TTTCTTCCTA GAGGTCATGG ATCTGANTCC GGANTTGTAC
  841 AGCTCGTCCA TGCCGANAGT GATCCCGGCG GCGGTCACGA ACTCCNGCNN NANNNTGTGA
  901 TCGCGCTTCN CGNNNGGGNC TTTGCTCNNG NCNGNNTGGG NNNTCNNNNA NNGGNTGTCG
961 GGNANCANCA CGGGNNNNNN NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB16878_A9A8c.seq check
>D06060F7_A9A8_3_CMV_Forward_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGAGCAA AGANCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN
  781 TTCGTGANCG CCGCCGGGNT CACTCTCGGC ATGGNCGANC TGTACAAGTN CGGANTCAGA
  841 TNCNTGANCT CTANGAAGAA AGTGTTGCTG AANGTCATCA TCCTGGGGNA NNNNNNNGNT
  901 GNAAAGNNNN CTCNNATGNA NCNNGTATGT GNANNNNAAN TTCAGTAANN NGTNNNAAGN
961 NNCNNNNNNA NCNGNNNNTT NNGANCNNNN ANNNNNNNGN NNGNNNNANN NNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16878_A9A8d.seq check
>D06060F7_A9A8_3_SV40pro_F_V2_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNTTTTGN AAGNNCGATC AAGAGANAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN
  781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG ANTTCTTCTG AGCGGGACTC TGGGGTTCGA ANTGACCGAC CAAGCGACGC
  901 CCAACCTGNC ATCACGAGAT TTCNANNNCN NCGCCGCNTT CTATGAAANG NNNGGNNTCN
  961 GNNNCNTTTT CCNGGNNNNN NGNNNGNNGA TNCTNNNNCG CNGGGNTNNN NNGNNNNNNN
1021 NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]