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pEGFP-C1-Rab7-T22N (#RDB16877)

Expression vector of mouse Rab7A constitutive negative mutant, T22N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab7A constitutive negative mutant, T22N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16877 pEGFP-C1-Rab7-T22N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab7-T22N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16877).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16877_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB16877_A9A8a.seq check
>D06060I5_A9A8_2_EGFP-C2_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNT CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGNTCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGACCTCT AGGAAGAAAG
  121 TGTTGCTGAA GGTCATCATC CTGGGGGACT CTGGTGTTGG AAAGAACTCT CTCATGAACC
  181 AGTATGTGAA CAAGAAGTTC AGTAACCAGT ACAAAGCCAC AATAGGAGCG GACTTTCTGA
  241 CCAAGGAGGT GATGGTGGAC GACAGACTTG TTACCATGCA GATCTGGGAC ACAGCCGGTC
  301 AAGAACGGTT CCAGTCTCTT GGTGTGGCCT TCTACAGAGG TGCAGATTGC TGTGTTCTGG
  361 TGTTTGATGT GACTGCCCCC AACACTTTCA AAACCCTCGA CAGCTGGAGA GACGAGTTTC
  421 TCATCCAGGC CAGCCCCCGG GATCCCGAGA ACTTCCCTTT TGTTGTGTTG GGAAACAAGA
  481 TTGACCTGGA AAACAGACAA GTGGCCACAA AGAGGGCACA GGCTTGGTGC TACAGCAAAA
  541 ACAACATTCC TTACTTCGAG ACCAGTGCCA AGGAGGCCAT CAATGTGGAG CAGGCCTTCC
  601 AGACAATTGC TCGGAATGCC CTTAAACAGG AAACAGAAGT GGAACTGTAC AATGAATTCC
  661 CTGAACCCAT CAAACTGGAC AAGANTGACC GGGCCAAGGC CTCCGCAGAA AGCTGCAGTT
  721 GTTGAAATCA CTAGTGAATT CGCGGCCGCC TGCANGTCGA CGGTACNGCG GGCCCGGGNN
  781 NCACCGGNTC TAGATAACTG ATCATAATCA GCCNTACCAC ATTTGTAGAN GTTTTACTTG
  841 CTTTAAAAAA CCTCCCNCAC CTCCCCNNNN ANNNGAANNA TAAANNGNAN GNNNNTGNNG
  901 NNGNTNACNN GNTNANNNNN NCNNNNNNNG GNNANNANTN ANNCNNNNNC NTCNNNANTT
961 TCNNNANNNA NNNNNTTTTT NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16877_A9A8b.seq check
>D06060I5_A9A8_2_SV40polyA-R2_B06_06_ABI24.ab1
    1 NNNNNNNNNC NNNNNNNNNN GTTAANNANN ACAATTGCAT TCNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACCTGCAGG
  181 CGGCCGCGAA TTCACTAGTG ATTTCAACAA CTGCAGCTTT CTGCGGAGGC CTTGGCCCGG
  241 TCATTCTTGT CCAGTTTGAT GGGTTCAGGG AATTCATTGT ACAGTTCCAC TTCTGTTTCC
  301 TGTTTAAGGG CATTCCGAGC AATTGTCTGG AAGGCCTGCT CCACATTGAT GGCCTCCTTG
  361 GCACTGGTCT CGAAGTAAGG AATGTTGTTT TTGCTGTAGC ACCAAGCCTG TGCCCTCTTT
  421 GTGGCCACTT GTCTGTTTTC CAGGTCAATC TTGTTTCCCA ACACAACAAA AGGGAAGTTC
  481 TCGGGATCCC GGGGGCTGGC CTGGATGAGA AACTCGTCTC TCCAGCTGTC GAGGGTTTTG
  541 AAAGTGTTGG GGGCAGTCAC ATCAAACACC AGAACACAGC AATCTGCACC TCTGTAGAAG
  601 GCCACACCAA GAGACTGGAA CCGTTCTTGA CCGGCTGTGT CCCAGATCTG CATGGTAACA
  661 AGTCTGTCGT CCACCATCAC CTCCTTGGTC AGAAAGTCCG CTCCTATTGT GGCTTTGTAC
  721 TGGTTACTGA ACTTCTTGTT CACATACTGG TTCATGAGAG AGTTCTTTCC NACACCAGAN
  781 TCCCCCANGA TGATGANCTT CAGCAACACT TTCTTCCTAG ANGTCATGGA TCTGANTNCN
  841 GANTTGTACA GCTCGTCCAT GNCGAGANTG ATNCNNGCGG CGGNCACNAA CTNCNGCNNN
  901 ANNNTNTGNN CGCNCTTCNC NNNNGGGTCT TNGCNCNNGN CGGNNTGGGN GCTCNNNNAN
961 NGGNTNNNCN GGNANCANCA CGGGGNCNNN CNNNNNNGGG GGGNNNNNNN NNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB16877_A9A8c.seq check
>D06060I5_A9A8_2_CMV_Forward_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNTTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
  721 CACCCAGTCC GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAN CTGTACAAGT CCGGANTCAG
  841 ATCCNTGACC TCTANGAAGA AAGTGTTGCT GAAGGTCATC ATCCTGGGGG NNTNNGGNGT
  901 TGNAAAGAAC TCTCTCNTGA ACCAGNATGT GAANNANAAN TTCNGTANCC NNNACAANGC
961 CNNNNNNNNA NNNNNNTTTN NGANNNNNNN NNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16877_A9A8d.seq check
>D06060I5_A9A8_2_SV40pro_F_V2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNGGCTTTNG NNNGNNCGAT CNAGAGACAG GATGAGGNTC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CANCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GANCGGNANT CNGGGGGTTC GAAATGANCG ANCNNNCGAC
  901 GCCCAACCTG CCNTCACGAN ANTTCNATTC CNNNGCCNNN TTCTATGAAA NGNNTGGGCT
  961 TNNGNNTCGN TTTNCNGNNN NNCNNNNNGG ATGATNNTNN NNNNCNGGNN NNNNNNGNNN
1021 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]