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pEGFP-C1-Rab6A-T27N (#RDB16875)

Expression vector of mouse Rab6A constitutive negative mutant, T27N, fused with EGFP at N-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ishida, M., J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse Rab6A constitutive negative mutant, T27N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Rab6a cDNA
Reference of insert sequence AB232596 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee
RDB16875 pEGFP-C1-Rab6A-T27N DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab6A-T27N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16875).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [PubMed] [Article] [RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [PubMed] [Article] [RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [PubMed] [Article] [RRC of NBRP]