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pEGFP-C1-Rab3A-Q81L (#RDB16871)

Expression vector of mouse Rab3A constitutive active mutant, Q81L, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab3A constitutive active mutant, Q81L, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab3a cDNA
Reference of insert sequence AB232587 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16871 pEGFP-C1-Rab3A-Q81L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab3A-Q81L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16871).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16871_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5' (Q81L)
Sequence file: RDB16871_A9A8a.seq check
>D06060E9_A9A8_2_EGFP-C2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNTC NCNTGGTCCT GCTGGANTTC GTGACCGCCG CCGGGNTCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTTCCG CCACAGACTC
  121 TCGCTATGGG CAGAAGGAGT CCTCAGACCA GAACTTCGAC TATATGTTCA AGATCCTGAT
  181 CATTGGGAAC AGCAGCGTGG GCAAAACCTC GTTCCTCTTC CGCTACGCAG ATGACTCCTT
  241 CACTCCAGCC TTTGTCAGCA CCGTTGGCAT AGACTTCAAG GTCAAAACCA TCTATCGCAA
  301 CGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGG CTAGAGCGGT ACCGCACCAT
  361 CACCACAGCC TATTACCGAG GCGCCATGGG CTTCATCCTA ATGTATGACA TCACCAATGA
  421 GGAGTCATTT AATGCAGTGC AGGACTGGTC CACTCAGATC AAAACTTACT CGTGGGACAA
  481 TGCCCAGGTG CTGCTGGTGG GAAACAAGTG TGACATGGAA GATGAGCGAG TGGTGTCCTC
  541 AGAGCGTGGC CGGCAGCTGG CTGACCACCT GGGCTTTGAG TTCTTTGAGG CCAGCGCCAA
  601 GGACAACATT AATGTCAAGC AGACGTTTGA ACGTCTGGTG GACGTGATCT GTGAGAAGAT
  661 GTCAGANTCC CTGGATACTG CAGACCCTGC GGTCACCGGT GCCAAGCANG GCCCGCAGCT
  721 CACCGACCAG CANGCGCCAC CTCATCANGA NTGTGCCTGC TGAAATCACT AGTGAATTCT
  781 GNAGNCGANG GTANCGCGGG CCCGGGNNNC ACCGGATCTA GATAACNGAT CATAATCAGC
  841 CNTANNNNNT TTGNANANGN TTNACTNGCT TTAAAAAANN NCCCNCNCCN CCCCCNGAAN
901 CTGAANNNTA AAATGAANGN NNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert (Q81L)
Sequence file: RDB16871_A9A8b.seq check
>D06060E9_A9A8_2_SV40polyA-R2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA GTTAANNANN ACAATTGCAT TCNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTCAGCAG GCACAATCCT GATGAGGTGG CGCCTGCTGG TCGGTGAGCT
  241 GCGGGCCCTG CTTGGCACCG GTGACCGCAG GGTCTGCAGT ATCCAGGGAC TCTGACATCT
  301 TCTCACAGAT CACGTCCACC AGACGTTCAA ACGTCTGCTT GACATTAATG TTGTCCTTGG
  361 CGCTGGCCTC AAAGAACTCA AAGCCCAGGT GGTCAGCCAG CTGCCGGCCA CGCTCTGAGG
  421 ACACCACTCG CTCATCTTCC ATGTCACACT TGTTTCCCAC CAGCAGCACC TGGGCATTGT
  481 CCCACGAGTA AGTTTTGATC TGAGTGGACC AGTCCTGCAC TGCATTAAAT GACTCCTCAT
  541 TGGTGATGTC ATACATTAGG ATGAAGCCCA TGGCGCCTCG GTAATAGGCT GTGGTGATGG
  601 TGCGGTACCG CTCTAGCCCT GCTGTGTCCC AGATCTGCAG CTTGATCCTC TTGTCGTTGC
  661 GATAGATGGT TTTGANCTTG AAGTCTATGC CAACGGTGCT GACAAAGGCT GGAGTGAANG
  721 AGTCATCTGC GTAGCGGAAG ANGAACGAGG TTTTGCCCAC GCTGCTGTTC CCAATGATCA
  781 GGANCTTGAA CATATAGTCG AAGTTCTGGT CTGANGANTC CTTCTGCCCA TAGCGAGANT
  841 CTGTGGCGGA AGCCATGGAT CTGANTNCGG ANTTGNNCAG CTCGNCCATG NCNANANTGA
  901 TNCCGGNGGN NGNCNCGAAC TCCAGCAGGA NCNTGTGNTC NCGCNTCNCG NNNGGNNCTT
  961 TGCTCNNNNC GNNNNGGNNN NNCNNNTANN NNNTNNNNGG GNANCANCNN NGGNNNNNNN
1021 NNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP
Sequence file: RDB16871_A9A8c.seq check
>D06060E9_A9A8_2_CMV_Forward_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 ANTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGNCGA NCTGTACAAG TCCGGANTCA
  841 GATCCATGGC TTCCGCCACA GACTCTCGCN ATGGGCAGAA NGANTCCTCA NANCAGAACT
  901 TCGANNATAT GTTCNNGATN NNGATCATTG GGAANAGCAG CGNGGGNAAA ACNNCNTNCC
  961 NNTTCCGNNN CNNAGANNNN NNCNTNNNNC CNNCNNTNNN CAGCNNNNNN NGNNNNNNNN
1021 NN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16871_A9A8d.seq check
>D06060E9_A9A8_2_SV40pro_F_V2_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CTTTTGCAAG NNCGATCAAG AGANAGGNNG AGGNNCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG NCGGCGAATG GGCTGANCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGANGAGT TCTTCTGANC GGGNNTCTGG GGGTNCGANN TGACCGACCN ANCGACGCCC
  901 NACCNGCCAT CACGANNNTT CGANNCCNNC GCNNCCNNCT ATGAAANGNT NGGNNTNCNN
961 NANCGNTTTN CNGGNNNNNC NNNNNNGNTG NNNNNNCNNN NNNGGGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]