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pEGFP-C1-Rab3A-T36N (#RDB16870)

Expression vector of mouse Rab3A constitutive negative mutant, T36N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab3A constitutive negative mutant, T36N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab3a cDNA
Reference of insert sequence AB232587 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16870 pEGFP-C1-Rab3A-T36N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab3A-T36N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16870).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16870_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5' (S36N)
Sequence file: RDB16870_A9A8a.seq check
>D06060E8_A9A8_3_EGFP-C2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNGN NCNCNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGNTC
   61 ACTCTCGGCA TGNNNNANNT GTACAAGTCC GGACTCAGAT CCATGGCTTC CGCCACAGAC
  121 TCTCGCTATG GGCAGAAGGA GTCCTCAGAC CAGAACTTCG ACTATATGTT CAAGATCCTG
  181 ATCATTGGGA ACAGCAGCGT GGGCAAGAAT TCGTTCCTCT TCCGCTACGC AGATGACTCC
  241 TTCACTCCAG CCTTTGTCAG CACCGTTGGC ATAGACTTCA AGGTCAAAAC CATCTATCGC
  301 AACGACAAGA GGATCAAGCT GCAGATCTGG GACACAGCAG GGCAAGAGCG GTACCGCACC
  361 ATCACCACAG CCTATTACCG AGGCGCCATG GGCTTCATCC TAATGTATGA CATCACCAAT
  421 GAGGAGTCAT TTAATGCAGT GCAGGACTGG TCCACTCAGA TCAAAACTTA CTCGTGGGAC
  481 AATGCCCAGG TGCTGCTGGT GGGAAACAAG TGTGACATGG AAGATGAGCG AGTGGTGTCC
  541 TCAGAGCGTG GCCGGCAGCT GGCTGACCAC CTGGGCTTTG AGTTCTTTGA GGCCAGCGCC
  601 AAGGACAACA TTAATGTCAA GCAGACGTTT GAACGTCTGG TGGACGTGAT CTGTGAGAAA
  661 ATGTCAGAGT CCCTGGATAC TGCAGACCCT GCGGTCACCG GTGCCAAGCA GGGCCCGCAG
  721 CTCACCGACC AGCAGGCGCC ACCTCATCAG GANTGTGCCT GCTGAAATCA CTAGTGAATT
  781 CTGCAGTCGA CGGTACCGCG GGCCCGGGNT CCACCGGATC TAGATAACTG ATCATAATCA
  841 GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCNNNNCACC TCCCCCCTGN
  901 ACCTGAAACA TAAAATGAAT GCNATTGTTG TTGTNNNCTT GTTTANTGCA NCTTATAANG
  961 GTTANAAATA AAGCAATANC ATCNCAAATT TCACAANTAA NNCNNTTTTT TTNNNNNNNN
1021 NN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert (S36N)
Sequence file: RDB16870_A9A8b.seq check
>D06060E8_A9A8_3_SV40polyA-R2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNN NAGTTAANNA CAACNATTGC ATTCNTTTTA TGTTTCNGGT
   61 TCNGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG
  181 AATTCACTAG TGATTTCAGC AGGCACAATC CTGATGAGGT GGCGCCTGCT GGTCGGTGAG
  241 CTGCGGGCCC TGCTTGGCAC CGGTGACCGC AGGGTCTGCA GTATCCAGGG ACTCTGACAT
  301 TTTCTCACAG ATCACGTCCA CCAGACGTTC AAACGTCTGC TTGACATTAA TGTTGTCCTT
  361 GGCGCTGGCC TCAAAGAACT CAAAGCCCAG GTGGTCAGCC AGCTGCCGGC CACGCTCTGA
  421 GGACACCACT CGCTCATCTT CCATGTCACA CTTGTTTCCC ACCAGCAGCA CCTGGGCATT
  481 GTCCCACGAG TAAGTTTTGA TCTGAGTGGA CCAGTCCTGC ACTGCATTAA ATGACTCCTC
  541 ATTGGTGATG TCATACATTA GGATGAAGCC CATGGCGCCT CGGTAATAGG CTGTGGTGAT
  601 GGTGCGGTAC CGCTCTTGCC CTGCTGTGTC CCAGATCTGC AGCTTGATCC TCTTGTCGTT
  661 GCGATAGATG GTTTTGACCT TGAAGTCTAT GCCAACGGTG CTGACAAAGG CTGGAGTGAA
  721 GGAGTCATCT GCGTAGCGGA AGAGGAACGA ATTCTTGCCC ACGCTGCTGT TCCCAATGAT
  781 CAGGATCTTG AACATATAGT CGAAGTTCTG GTCTGANGAN TCCTTCTGCC CATAGCGAGA
  841 GTCTGTGGCG GAAGCCATGG ATCTGANNCC GGANNTGTAC AGCTCGTCCA TGCNGANANT
  901 GATCCNGGCG GCGGTCNNGA ACTCCAGCAN GNNNNTGTGA TCGCGCTTCN CNTTGGGGTC
  961 TTTGCTCNNN NCNNANNGGG NGNNCNNNTA NNGGNTTGNN NGGCANCAGC NCGGGGNCNN
1021 NNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP
Sequence file: RDB16870_A9A8c.seq check
>D06060E8_A9A8_3_CMV_Forward_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA
  721 CCCAGTCCGC CCTGANCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGNCGANCT GTACAAGTCC GGANTCAGAT
  841 CCATGGCTTC CGCCACAGAC TCTCGCTATG GGCAGAANGA NNNCNCAGAN NAGAACTTCG
  901 ANTATATGTN CAANATNNNG ATCATTGGGA ACAGCAGCGN GGGNNAGANN NCGTNCCNNT
  961 NNCGNNACGC ANANGANNNN NNCNNNCCNG CNNTTNNNAN CNNNNNTNNG NNNNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16870_A9A8d.seq check
>D06060E8_A9A8_3_SV40pro_F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNGGNTTTTG NAAGNNCGAT CANNGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAANA NCTTGNCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA NGAGTTCTTC TGANCGGGNN TCTGGGGNNC GAAATGACCG ACCAAGCGAN
  901 GCCNANCCTG NCNTCNCGAN NNTTCNATTC CACCGCCGCC TNCNNTNNNN NNNNGGCTTC
961 NGNANNCNTT TNNNNGNNNN NNGGCTNNNN GATNCNNNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]