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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-4T-3-Rab23 (#RDB16847)

Expression vector of mouse Rab23 tagged with GST at N-terminus, tac promoter.

Clone info. Expression vector of mouse Rab23 tagged with GST at N-terminus, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab23 cDNA
Reference of insert sequence AB232618 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。

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RDB16847 pGEX-4T-3-Rab23 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-Rab23 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16847).

Reference section:

Itoh, T., Satoh, M., Kanno, E., Fukuda, M., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16847_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST 3',insert 5'
Sequence file: RDB16847_A9A8a.seq check
>16847_16847_B1JdF_2_pGEX-F2_A01_01_ABI08.ab1
    1 NNNNNNTTNN NNNNNNTATT GAAGCTATCC CNCAAATTGA TAAGTACTTG AAATCCAGCA
   61 AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC
  121 CAAAATCGGA TCTGGTTCCG CGTGGATCCA TGTTGGAGGA AGATATGGAA GTGGCCATCA
  181 AGATGGTGGT GGTGGGGAAT GGGGCGGTTG GAAAGTCGAG CATGATTCAG CGCTACTGCA
  241 AAGGCATTTT CACAAAAGAC TACAAGAAAA CCATCGGCGT AGACTTTTTG GAGCGACAGA
  301 TTCAGGTTAA CGATGAAGAT GTAAGGCTAA TGTTATGGGA CACTGCAGGT CAAGAGGAGT
  361 TTGATGCAAT CACGAAGGCC TACTATCGAG GAGCCCAGGC TTGTGTGCTT GTGTTTTCTA
  421 CCACAGACAG GGAATCTTTT GAAGCGATTT CCAGCTGGAG AGAAAAAGTA GTAGCGGAGG
  481 TTGGAGATAT ACCAACCGCA CTGGTGCAAA ACAAGATCGA TCTGCTGGAT GATTCATGCA
  541 TCAAAAATGA GGAAGCGGAN GGACTGGCCA AAAGGCTAAA GCTGANGTTC TACCGGACGT
  601 CCGTGAAGGA NGANCTCNAC GTGAGTGAAG TTTTTNAATA TTTGGCTGAN AAACNCCTAC
  661 AAAAACTCNA ACNACNAATA ACCGAANANN CNNNNNGANN CNCTCNAGTA NNNNNNNNAN
721 TGGNGNNNTN NNGNNTCNGN NCNNNNNTCN NNNNNNNNN
//
Primer: pGEX3' (Pr0213)
Region: insert 3'
Sequence file: RDB16847_A9A8b.seq check
>16847_16847_B1JdF_2_pGEX3'_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNANCG TNNGTCNGTC ACGATGCGGC CGCGAATTCA
   61 CTAGTGATTT AGGGTACACT ACAGCTGCTG AAAGGGTTTC TGGTTCTCTT GGTCCTTTGT
  121 TTGTTAGGTC TAAGGTTGAT GACATCGCCA CCATTAAGGG AACTTGAATT CTGGCCCAAG
  181 TGACTTCCGA CCGATGCATT AAAGACACCA ATCTTGTTAC TACTTGAGTG TGTCTGTTCT
  241 GGATCTTCGG TTATTTGTTG TTTGAGTTTT TGTAGGTGTT TCTCAGCCAA ATATTTAAAA
  301 ACTTCACTCA CGTTGAGGTC CTCCTTCACG GACGTCCGGT AGAACCTCAG CTTTAGCCTT
  361 TTGGCCAGTC CCTCCGCTTC CTCATTTTTG ATGCATGAAT CATCCAGCAG ATCGATCTTG
  421 TTTTGCACCA GTGCGGTTGG TATATCTCCA ACCTCCGCTA CTACTTTTTC TCTCCAGCTG
  481 GAAATCGCTT CAAAAGATTC CCTGTCTGTG GTAGAAAACA CAAGCACACA AGCCTGGGCT
  541 CCTCGATAGT AGGCCTTCGT GATTGCATCA AACTCCTCTT GACCTGCAGT GTCCCATAAC
  601 ATTAGCCTTA CNTCTTCATC GTTAACCTGA ATCTGTCGCT CCNAAAANTC TACGCCGANG
  661 GTTTTCTTGT ANNCTTTTGN GAAAATGCCT TTGCAGNANC GCTNANCNNG CTCNANTTTN
721 CNNNNGCCCN NNNCCCNNNN NNNNNN
//
Primer: pGEX-ptac_up-F (Pr0455)
Region: lacI pro,lac I
Sequence file: RDB16847_A9A8c.seq check
>16847_16847_B1JdF_2_pGEX-ptac_up-F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCGG TTCTGNNNNN NNTCTGAAAT GAGCTGTTGA CAATTAATCA
   61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT
  121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT
  181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA
  241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA
  301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA
  361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT
  421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT
  481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA
  541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA
  601 TGTTGTTTTA TACATGGACC CNATGTGCCT GGATGCGTTC CCNAAATTAN TTTGNTTTNA
  661 AAANNGTATT GAAGCTATCC NNCAAATTGA TAANNACTTG AAATNCNNCN NGNANNTANN
721 ANNNNNNTNN NNNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: tac pro,lac O,GST 5'
Sequence file: RDB16847_A9A8d.seq check
>16847_16847_B1JdF_2_lacIpro-B_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNCNN NTCNGTGAGC GAGGNAGCGG AAGAGCGCCT GATGCGGTAT
   61 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAA ATTCCGACAC CATCGAATGG
  121 CGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG
  181 AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC
  241 GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA
  301 GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA
  361 CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT
  421 GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA
  481 GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC
  541 AGTGGGCTGA TCATTAACTA TCCGCTGGAT GANCAGGATG CCATTGCTGT GGAAGCTGCC
  601 TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGANN NGACACCCNT CNACAGTATT
  661 ATTTTCTCCC NNGAANANNN NACGCNACNG GGNNNNGNNC NNCNGGNCGC ATTNGGNCNN
721 CNNCANNCNC GCNNNNANNN GGNNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]