RDB16803 - pCAG-NexxoR

pCAG-NexxoR (#RDB16803)

Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Clone info.	Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells. For the selection of sgRNA on double-strand breaks by Neomycin (or G418) resistance. MCS: BamHI, NheI, PstI, SalI, EcoRI, and EcoRV. This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013).
Vector backbone	pCAGGS (Plasmid)
Selectable markers	Amp^r
Growth conditions	37oC, LB + Amp.
Depositor\|Developer	Hashido, Kazuo \| Terumitsu, Mika \|

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, description of “This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013)” is requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, description of “This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013)” is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とする。利用者は研究成果の公表に当たって、本件リソースがpCAG-EGxxFP (Mashiko, O.,Sci. Rep. 3: 3355, 2013) を基に作成したことを記すこと。

Catalog #	Resource name	Shipping form	Fee
RDB16803	pCAG-NexxoR	DNA solution

Materials & Methods section:

The pCAG-NexxoR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16803).

Reference section:

Original, user report and related articles

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External Database

Reference sequence