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pCAG-NexxoR

Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells.

Catalog number RDB16803
Resource name pCAG-NexxoR
Clone info. Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells. For the selection of sgRNA on double-strand breaks by Neomycin (or G418) resistance. MCS: BamHI, NheI, PstI, SalI, EcoRI, and EcoRV. This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013).
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Depositor Hashido, Kazuo | Terumitsu, Mika |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, description of “This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013)” is requested.
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RDB16803 pCAG-NexxoR DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0098j (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16803_A8Llp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F | Sequence: RDB16803_A8Lla.seq
>D06142A1_A8Ll_1_pCAG-F_A05_01_ABI08.ab1
    1 ATCTGGGGGG GCAGCTGGCC TTCGGGGGGG GGAGGGGCAG GGCGGGGTTC GGCTTCTGGC
   61 GTGTGACCGG CGGCTCTAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG
  121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAATC TAGAGCCGCC
  181 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  241 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  301 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  361 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  421 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  481 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  541 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
  601 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
  661 GAGCATCAGG GGCTCGCGCC AGCCGGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
  721 CGGCGAGGAT CTCGTCGTGA CCCATGGCGT GAGGATCCGC TAGCCTGCAG GTCGACGAAT
  781 TCGATATCTA GAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC
  841 GGCTATCGTG GCTGGGCCAC GACGGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
  901 TGAAAGCGGG GAAGGGACTG GCTTGCCTAT TGGGGCGGAA GTGCCCGGGG CAGGATCTTC
  961 CTTGTCAATC CTCACCTTGG CTTCCTGGCC GGAGAAAGGT ATTCCATCAA TGGGCCTGAA
 1021 TGCAATTGCC GGGCGGCTGC ATAACGCTGA TCCGGCTAAC TTGGCCCAAT TCGGAACCAC
 1081 TAGGCGAAAC CAATCGCATC GAAGCGGAAG CTACGGTTAC CTCGAATGAG GCGCGGTCTA
1141 TGATCCGAA
//
Primer: pCA-bgter | Sequence: RDB16803_A8Llb.seq
>D06142A1_A8Ll_1_pCA-bgter_B05_02_ABI08.ab1
    1 AGCCCAGGGG AAGTCCAATA ATTTTTGGCA GAGGGAAAAG ATCTCAGTGG TATTTGTGAG
   61 CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC TGAGGAGTCT
  121 CGAGCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG
  181 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC
  241 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA
  301 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC
  361 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC
  421 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC
  481 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC
  541 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG
  601 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT
  661 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA
  721 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTCTA
  781 GATATCGAAT TCGTCGACCT GCAGGCTAGC GGATCCTCAC GCCATGGGTC ACGACGAGAT
  841 CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT
  901 GATGCTCTTC GTCCAGATCA TCCCTGATCG ACAGACCGGG CTTCCCATCC GAGTACGTGC
961 TCGCTCGATG CGATGTTTTC CGCCTTGGGT GGTCGAATGG G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.19

GNP_filter3_RDBDEP_html_190816.pl