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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-NexxoR (#RDB16803)

Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells.

Clone info. Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells. For the selection of sgRNA on double-strand breaks by Neomycin (or G418) resistance. MCS: BamHI, NheI, PstI, SalI, EcoRI, and EcoRV. This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013).
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Depositor|Developer Hashido, Kazuo | Terumitsu, Mika |
 

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, description of “This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013)” is requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。利用者は研究成果の公表に当たって、本件リソースがpCAG-EGxxFP (Mashiko, O.,Sci. Rep. 3: 3355, 2013) を基に作成したことを記すこと。

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RDB16803 pCAG-NexxoR DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-NexxoR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16803).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16803_A8Llp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328)
Region: CAG pro,insert 5'(NexxoR-N 5')
Sequence file: RDB16803_A8Llc.seq check
>16803_16803_B2LrF_pCAG-F_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGNAGGG CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
  121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAATCTA GAGCCGCCAT
  181 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
  241 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
  301 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
  361 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
  421 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
  481 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
  541 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
  601 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
  661 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG
  721 CGAGGATCTC GTCGTGACCC ATGGCGTGAG GATCCGCTAG CCTGCAGGTC GACGAATTCG
  781 ATATCTAGAA GACCGACCTG TCCGGTGCCC TGAATGANCT GCAAGACGAG GCAGCGCGGC
  841 TATCGTGNCT GGNCNCGACG GGCGTTCCTT GCGCNGCTGT GCTCGACGTT GTCACTGAAN
  901 CNGNAAGGGN NTGGCTGCTA TTGNNNNAAN TGNCNGGGCA GGATNNNCTG TNNTCTCNNC
  961 TNGNTCCTGC CNANAANNNA TCCATCANGN NTGANGNNAN GCNNCNGNNG CATACNCNTG
 1021 ATNNGGNNAN NNNNCCNNTC NANNNNNNNG CGAAANNTNN NANNNNNNCG ANNNNNTNNN
1081 NNNNNNGNAN NNNNNNNNN
//
Primer: pCA-bgter (Pr0066)
Region: insert 3'(NexxoR-C)
Sequence file: RDB16803_A8Lld.seq check
>16803_16803_B2LrF_pCA-bgter_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNTTTGNNN NAGGGNNNAA GNNCTCAGTG GTATTTGTGA GCCAGGGCAT
   61 TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTC TCGAGCCCGC
  121 TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT
  181 ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG
  241 GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC AGTCGATGAA
  301 TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC
  361 GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC
  421 GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT
  481 ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG
  541 CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG
  601 AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC
  661 AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGNNCAGCC ACGATAGCCG
  721 CGCTGCCTCG TCTTGNAGTT CANTCANGGC ACCGGANAGG NCGGTCTTCT AGATATCGAA
  781 NTCGTCGANC TGCANGCTAG CGNATCCTCA CGCCNTGGGT CACGACGANA TNCTCGCCGN
  841 CNGGNATGCT CGCCNTGAGC CTGGCGAANA GTTCGGCTGG NGCGAGCCCC NGANGNTCTT
  901 CGTCCNNANC ATCCTGNNCN ANNANANNGG NTTNNNTCCN ANNANNNNNN NNCTCNATGN
961 NANGNTNCNC NNNNNGNCNN NNGGNNNNNN ANNNNNNNNN N
//
Primer: xoR-M_R (Pr 1277)
Region: insert mid(NexxoR-C,MCS,NexxR-N 3')
Sequence file: RDB16803_A8Lle.seq check
>16803_16803_B2LrF_xoR-M_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC
   61 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC
  121 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC
  181 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG
  241 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT
  301 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA
  361 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTCTA
  421 GATATCGAAT TCGTCGACCT GCAGGCTAGC GGATCCTCAC GCCATGGGTC ACGACGAGAT
  481 CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT
  541 GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC
  601 GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA
  661 GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA
  721 GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA
  781 CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT
  841 CGTCTTGNAG TTCATTCNNG GCACCGGACA GGTCGGTCTT GANAAAAAGA ACCGGGCGCC
  901 CCTGCGCTGA NAGCCGGAAC ACGGCGGCNT CNNANCANCC GANTGNCTGN TGTGNCCNGT
  961 CNTANNNNAN TAGCCTCTNN ACCCAAGCGG NCNGNANAAN NTGNGNGNNN TCCNTCNTNG
1021 NNCNNTCNNN GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles