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pCMV-eMvChR1#2(A97D)-Venus

Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2). A97D mutation was introduced.

Catalog number RDB16789
Resource name pCMV-eMvChR1#2(A97D)-Venus
Clone info. Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97D mutation was introduced.
Comment eMvChR1#2 consists of Met1-Ala37 of channelrhodopsin 2 from Chlamydomonas reinhardtii (CrChR2) and His57-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1). Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop2 (cop4) cDNA Mesostigma viride MvChR1 cDNA
Depositor|Developer Ishizuka, Toru |
Other clones in our bank

External Database
Chlamydomonas reinhardtii acop2 (cop4) Mesostigma viride MvChR1

          Reference sequence
            AB058891,AF461397,JF922293

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          付加的提供条件 Venus の使用について、宮脇敦史博士の書面による提供承諾を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB16789 pCMV-eMvChR1#2(A97D)-Venus Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Watanabe, S., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genetic Resources for Optgenetics Research (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Watanabe, S., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505.

          2022.05.17

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