Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2). A97D mutation was introduced.
Clone info. | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97D mutation was introduced. |
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Comment | eMvChR1#2 consists of Met1-Ala37 of channelrhodopsin 2 from Chlamydomonas reinhardtii (CrChR2) and His57-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1). Expression was confirmed by the depositor with whole-cell patch-clamp recording. |
Vector backbone | pVenus-N1 (plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
Growth conditions | LB+Kan, 37oC |
Gene/insert name | Chlamydomonas reinhardtii acop2 (cop4) cDNA Mesostigma viride MvChR1 cDNA |
Depositor|Developer | Ishizuka, Toru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 付加的使用条件: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB16789 | pCMV-eMvChR1#2(A97D)-Venus | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCMV-eMvChR1#2(A97D)-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16789). |
Reference section:
Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Watanabe, S., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP] |
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