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pCMV-I6K1NaR-3.0-EYFP

Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex).

Catalog number RDB16785
Resource name pCMV-I6K1NaR-3.0-EYFP
Clone info. Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
Comment A portion of the plasmid (TS+eYFP+ERex) was derived from a product provided by Dr. K Deisseroth (Gradinaru, V. et al., Cell 141: 154-165, 2010). Expression was confirmed by the depositor with whole-cell patch-clamp recording. tdTomato is not included.
Vector backbone ptdTomato-N1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Indibacter alkaliphilus IaNaR cDNA Krokinobacter eikastus KR2 (codon optimized for human) cDNA Aequorea victoria GFP (eYFP) cDNA
Reference of insert sequence LC187670 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |
Other clones in our bank

External Database
Indibacter alkaliphilus IaNaR
    Krokinobacter eikastus KR2 (codon optimized for human)
      Aequorea victoria GFP (eYFP)

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB16785 pCMV-I6K1NaR-3.0-EYFP Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Hoque, M.R., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Genetic Resources for Optgenetics Research (English text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Hoque, M.R., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619.

            2022.07.13

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