Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex).
Clone info. | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
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Comment | A portion of the plasmid (TS+eYFP+ERex) was derived from a product provided by Dr. K Deisseroth (Gradinaru, V. et al., Cell 141: 154-165, 2010). Expression was confirmed by the depositor with whole-cell patch-clamp recording. tdTomato is not included. |
Vector backbone | ptdTomato-N1 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | Indibacter alkaliphilus IaNaR cDNA Krokinobacter eikastus KR2 (codon optimized for human) cDNA Aequorea victoria GFP (eYFP) cDNA |
Reference of insert sequence | LC187665 (DDBJ accession number) |
Depositor|Developer | Ishizuka, Toru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB16780 | pCMV-I1K6NaR-3.0-EYFP | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pCMV-I1K6NaR-3.0-EYFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16780). |
Reference section:
Hoque, M.R., Ishizuka T., Inoue K., Abe-Yoshizumi, R., Igarashi, H., Mishima, T., Kandori, H., Yawo, H., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Hoque, M.R., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619. [link to RRC of NBRP] |
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