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pCMV-IaNaR-3.0-EYFP (#RDB16779)

Mammalian expression vector encoding a light-driven sodiun ion pump from Indibacter alkaliphilus (IaNaR) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex).

Clone info. Mammalian expression vector encoding a light-driven sodiun ion pump from Indibacter alkaliphilus (IaNaR) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
Comment A portion of the plasmid (TS+eYFP+ERex) was derived from a product provided by Dr. K Deisseroth (Gradinaru, V. et al., Cell 141: 154-165, 2010). Expression was confirmed by the depositor with whole-cell patch-clamp recording. tdTomato is not included.
Vector backbone ptdTomato-N1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Indibacter alkaliphilus IaNaR cDNA Aequorea victoria GFP (eYFP) cDNA
Reference of insert sequence LC187664 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoque, MR. et al., PLoS ONE, 11(11): e0166820, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。

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RDB16779 pCMV-IaNaR-3.0-EYFP Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pCMV-IaNaR-3.0-EYFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16779).

Reference section:

Hoque, M.R., Ishizuka T., Inoue K., Abe-Yoshizumi, R., Igarashi, H., Mishima, T., Kandori, H., Yawo, H., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hoque, M.R., A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. PLoS One 11 (11): e0166820 (2016). PMID 27861619. [link to RRC of NBRP]