RDB16776 - pCMV-ChRGR-Venus

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icon below as well as clone information before placing your order.

pCMV-ChRGR-Venus (#RDB16776)

Mammalian expression vector encoding a mammalian codon-optimized chopGR.

Clone info.	Mammalian expression vector encoding a mammalian codon-optimized chopGR (channelopsin/green-receiver) tagged with Venus at the C-terminus under CMV promoter.
Comment	Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone	pVenus-N1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions	LB+Kan, 37oC
Gene/insert name	Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of insert sequence	LC415029 (DDBJ accession number)
Depositor\|Developer	Ishizuka, Toru \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wen, L. et al., PLoS ONE 5 (9): e12893, 2010)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wen, L. et al., PLoS ONE 5 (9): e12893, 2010)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16776	pCMV-ChRGR-Venus	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-ChRGR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16776).

Reference section:

Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16776_A8L5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward () Sequence file: RDB16776_A8L5a.seq
>D06115A8_A8L5_2_CMV_Forward_E01_13_ABI24.ab1 1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC 121 AAGCTTCGAA TTCGCCACCA TGGCTCGGAG ACCTTGGCTG CTGGCACTGG CTCTGGCTGT 181 CGCTCTGGCC GCTGGCTCTG CTGGGGCTTC TACTGGATCT GACGCAACAG TGCCCGTCGC 241 TACTCAGGAC GGACCTGATT ACGTGTTCCA CAGGGCACAT GAGCGCATGC TGTTTCAGAC 301 ATCTTATACT CTGGAAAACA ATGGCAGTGT CATCTGCATT CCCAACAATG GGCAGTGCTT 361 CTGTCTGGCA TGGCTGAAGT CTAACGGCAC CAATGCCGAG AAACTGGCCG CTAACATCCT 421 GCAGTGGATT ACATTCGCCC TGTCCGCTCT GTGCCTGATG TTTTACGGAT ATCAGACCTG 481 GAAGTCTACA TGTGGCTGGG AGGAAATCTA CGTGGCTACC ATCGAGATGA TCAAGTTCAT 541 CATTGAATAC TTCCACGAGT TTGACGAACC TGCCGTGATC TATAGCTCCA ACGGGAATAA 601 GACTGTCTGG CTGAGATACG CTGAGTGGCT GCTGACCTGT CCAGTGATCC TGATTCATCT 661 GAGCAACCTG ACTGGCCTGG CCAACGACTA TAATAAGAGA ACCATGGGGC TGCTGGTGAG 721 TGATATCGGA ACAATTGTCT GGGGCACCAC AGCAGCCCTG TCAAAAGGCT ATGTGCGCGT 781 CATCTTCTTT CTGATGGGCC TGTGCTACGG GATCTACACA TTCTTTAATG CTGCAAAGGT 841 GTACATCGAG GCATATCACA CAGTCCCTAA AGGGCGGTGT AGACAGGTGG TCACTGGAAT 901 GGCCTGGCTG TTCTTTGTGT CCTGGGGGAT GTTCCCAATC CTGTTTCTGC TGGGACCCGA 961 AAGGATTCGG CCACATTAAC CAGTTTAATT CAGCCCATCG CTCATGCAAT TCTGGACCTG 1021 GCCCAGCAAA AACTGCTGGG GGGGCTGCTT GGGACACTAC CCTGCGAGTG CTGATCACGA 1081 AGCCATATCT TGAATTCATG ACGAATAATT CGGAAGAACT TAA //
Primer: _Venus-N_R () Sequence file: RDB16776_A8L5b.seq
>D06115A8_A8L5_2_Venus-N_R_F01_16_ABI24.ab1 1 CTGTCCGGGA CGCTGACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA 61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCTTCC 121 CAGTCCCTTT GTTCACGGCT CCTGCCTCGG CTTCATCTTC CACCAGTGTT TCCACCTCAA 181 TTTCGGTCCC GCCGATATTC AGTTTGGTAG TCTTCCGAAT ATCGCCATGA ATCAGGATAT 241 GCTCGTGGAT CAGCACTCGC AGGTAGTGTC CCAGCAGCCC CCAGCAGTTT TTGCTGGCCA 301 GGTCCAGAAT TGCATGAGCG ATGGCTGAAT TAAACTGGTT AATGTGGCCG AATCCTTCGG 361 GTCCCAGCAG AAACAGGATT GGGAACATCC CCCAGGACAC AAAGAACAGC CAGGCCATTC 421 CAGTGACCAC CTGTCTACAC CGCCCTTTAG GGACTGTGTG ATATGCCTCG ATGTACACCT 481 TTGCAGCATT AAAGAATGTG TAGATCCCGT AGCACAGGCC CATCAGAAAG AAGATGACGC 541 GCACATAGCC TTTTGACAGG GCTGCTGTGG TGCCCCAGAC AATTGTTCCG ATATCACTCA 601 CCAGCAGCCC CATGGTTCTC TTATTATAGT CGTTGGCCAG GCCAGTCAGG TTGCTCAGAT 661 GAATCAGGAT CACTGGACAG GTCAGCAGCC ACTCAGCGTA TCTCAGCCAG ACAGTCTTAT 721 TCCCGTTGGA GCTATAGATC ACGGCAGGTT CGTCAAACTC GTGGAAGTAT TCAATGATGA 781 ACTTGATCAT CTCGATGGTA GCCACGTAGA TTTCCTCCCA GCCACATGTA GACTTCCAGG 841 TCTGATATCC GTAAAACATC AGGCACAGAG CGGACAGGGC GAATGTAATC CACTGCAGGA 901 TGTTAGCGGC CAGTTTCTCG GCATTGGTGC CGTTAGACTT CAGCCATGCC AGACAGAAGC 961 ACTGCCCATT GTTGGGAAAT GCAGATGACA CTGCCATTGT TTTCCAGAGT ATAAGATGTC 1021 TGAAACAGCA TGCGCTCATG TGCCCTTGTT GGAACACGGT AAATCATGTT CCGATCTGAG 1081 TTAGCGGAAC GGGTAACTGA TTGCGTCAGG AATTCCAGT //
Primer: SV40polyA-R () Sequence file: RDB16776_A8L5c.seq
>D06115A8_A8L5_2_SV40polyA-R_G01_19_ABI24.ab1 1 AAAAATGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA 61 GTCGCGGCCG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG 301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG 361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG 601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCCG GTGGTGCAGA TCAGCTTCAG 661 GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG CCCTCGCCCG GACACGCTGA ACTTGTGGCC 721 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC 781 CTTGCTCACC ATGGTGGCGA CCGGTGGATC CTTTCCAGTT CCTTTGTTCC ACGCTTCTTG 841 CCTCGCTTTC ATCTTTCCAC CAGTGTTTCC ACCCTCAATT TCGGTCCCGC CGAATATTCA 901 GTTTGTTAGG TCTTCGAATT ATCGCATGAA TCAGGAATAT GTCTTCGGTG GAATACAAGC 961 ACCTCGCAGT TATTGTCAGC AGCTCTCAGC AGTTTGCTTG CAAGTCGGAA TTGACTGACC 1021 AATGCCTGAT ACCTGGTTAA TTGGCCGAAT CTCCGGTCCC AGCCGA //
Primer: SV40pro_F Sequence file: RDB16776_A8L5a.seqd.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]
protocol	Asano, T., Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells. Methods Mol. Biol. 1668: 135-145 (2017). PMID 28842907.

Resource search

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Other resources in our bank

External Database
Chlamydomonas reinhardtii acop1 (channelopsin-1)

in NCBI Gene

Chlamydomonas reinhardtii cop4 (acop2)

in NCBI Gene

Reference sequence