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pCMV-ChRGR-Venus

Mammalian expression vector encoding a mammalian codon-optimized chopGR.

Catalog number RDB16776
Resource name pCMV-ChRGR-Venus
Clone info. Mammalian expression vector encoding a mammalian codon-optimized chopGR (channelopsin/green-receiver) tagged with Venus at the C-terminus under CMV promoter.
Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of nucleotide sequence LC415029 (DDBJ accession number)
Depositor Ishizuka, Toru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16776 pCMV-ChRGR-Venus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genetic Resources for Optgenetics Research (English text)

References

original Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16776_A8L5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB16776_A8L5a.seq
>D06115A8_A8L5_2_CMV_Forward_E01_13_ABI24.ab1
    1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC
  121 AAGCTTCGAA TTCGCCACCA TGGCTCGGAG ACCTTGGCTG CTGGCACTGG CTCTGGCTGT
  181 CGCTCTGGCC GCTGGCTCTG CTGGGGCTTC TACTGGATCT GACGCAACAG TGCCCGTCGC
  241 TACTCAGGAC GGACCTGATT ACGTGTTCCA CAGGGCACAT GAGCGCATGC TGTTTCAGAC
  301 ATCTTATACT CTGGAAAACA ATGGCAGTGT CATCTGCATT CCCAACAATG GGCAGTGCTT
  361 CTGTCTGGCA TGGCTGAAGT CTAACGGCAC CAATGCCGAG AAACTGGCCG CTAACATCCT
  421 GCAGTGGATT ACATTCGCCC TGTCCGCTCT GTGCCTGATG TTTTACGGAT ATCAGACCTG
  481 GAAGTCTACA TGTGGCTGGG AGGAAATCTA CGTGGCTACC ATCGAGATGA TCAAGTTCAT
  541 CATTGAATAC TTCCACGAGT TTGACGAACC TGCCGTGATC TATAGCTCCA ACGGGAATAA
  601 GACTGTCTGG CTGAGATACG CTGAGTGGCT GCTGACCTGT CCAGTGATCC TGATTCATCT
  661 GAGCAACCTG ACTGGCCTGG CCAACGACTA TAATAAGAGA ACCATGGGGC TGCTGGTGAG
  721 TGATATCGGA ACAATTGTCT GGGGCACCAC AGCAGCCCTG TCAAAAGGCT ATGTGCGCGT
  781 CATCTTCTTT CTGATGGGCC TGTGCTACGG GATCTACACA TTCTTTAATG CTGCAAAGGT
  841 GTACATCGAG GCATATCACA CAGTCCCTAA AGGGCGGTGT AGACAGGTGG TCACTGGAAT
  901 GGCCTGGCTG TTCTTTGTGT CCTGGGGGAT GTTCCCAATC CTGTTTCTGC TGGGACCCGA
  961 AAGGATTCGG CCACATTAAC CAGTTTAATT CAGCCCATCG CTCATGCAAT TCTGGACCTG
 1021 GCCCAGCAAA AACTGCTGGG GGGGCTGCTT GGGACACTAC CCTGCGAGTG CTGATCACGA
1081 AGCCATATCT TGAATTCATG ACGAATAATT CGGAAGAACT TAA
//
Primer: Venus-N_R RDB16776_A8L5b.seq
>D06115A8_A8L5_2_Venus-N_R_F01_16_ABI24.ab1
    1 CTGTCCGGGA CGCTGACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA
   61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCTTCC
  121 CAGTCCCTTT GTTCACGGCT CCTGCCTCGG CTTCATCTTC CACCAGTGTT TCCACCTCAA
  181 TTTCGGTCCC GCCGATATTC AGTTTGGTAG TCTTCCGAAT ATCGCCATGA ATCAGGATAT
  241 GCTCGTGGAT CAGCACTCGC AGGTAGTGTC CCAGCAGCCC CCAGCAGTTT TTGCTGGCCA
  301 GGTCCAGAAT TGCATGAGCG ATGGCTGAAT TAAACTGGTT AATGTGGCCG AATCCTTCGG
  361 GTCCCAGCAG AAACAGGATT GGGAACATCC CCCAGGACAC AAAGAACAGC CAGGCCATTC
  421 CAGTGACCAC CTGTCTACAC CGCCCTTTAG GGACTGTGTG ATATGCCTCG ATGTACACCT
  481 TTGCAGCATT AAAGAATGTG TAGATCCCGT AGCACAGGCC CATCAGAAAG AAGATGACGC
  541 GCACATAGCC TTTTGACAGG GCTGCTGTGG TGCCCCAGAC AATTGTTCCG ATATCACTCA
  601 CCAGCAGCCC CATGGTTCTC TTATTATAGT CGTTGGCCAG GCCAGTCAGG TTGCTCAGAT
  661 GAATCAGGAT CACTGGACAG GTCAGCAGCC ACTCAGCGTA TCTCAGCCAG ACAGTCTTAT
  721 TCCCGTTGGA GCTATAGATC ACGGCAGGTT CGTCAAACTC GTGGAAGTAT TCAATGATGA
  781 ACTTGATCAT CTCGATGGTA GCCACGTAGA TTTCCTCCCA GCCACATGTA GACTTCCAGG
  841 TCTGATATCC GTAAAACATC AGGCACAGAG CGGACAGGGC GAATGTAATC CACTGCAGGA
  901 TGTTAGCGGC CAGTTTCTCG GCATTGGTGC CGTTAGACTT CAGCCATGCC AGACAGAAGC
  961 ACTGCCCATT GTTGGGAAAT GCAGATGACA CTGCCATTGT TTTCCAGAGT ATAAGATGTC
 1021 TGAAACAGCA TGCGCTCATG TGCCCTTGTT GGAACACGGT AAATCATGTT CCGATCTGAG
1081 TTAGCGGAAC GGGTAACTGA TTGCGTCAGG AATTCCAGT
//
Primer: SV40polyA-R RDB16776_A8L5c.seq
>D06115A8_A8L5_2_SV40polyA-R_G01_19_ABI24.ab1
    1 AAAAATGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA
   61 GTCGCGGCCG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG
  301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG
  361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG
  601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCCG GTGGTGCAGA TCAGCTTCAG
  661 GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG CCCTCGCCCG GACACGCTGA ACTTGTGGCC
  721 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC
  781 CTTGCTCACC ATGGTGGCGA CCGGTGGATC CTTTCCAGTT CCTTTGTTCC ACGCTTCTTG
  841 CCTCGCTTTC ATCTTTCCAC CAGTGTTTCC ACCCTCAATT TCGGTCCCGC CGAATATTCA
  901 GTTTGTTAGG TCTTCGAATT ATCGCATGAA TCAGGAATAT GTCTTCGGTG GAATACAAGC
  961 ACCTCGCAGT TATTGTCAGC AGCTCTCAGC AGTTTGCTTG CAAGTCGGAA TTGACTGACC
1021 AATGCCTGAT ACCTGGTTAA TTGGCCGAAT CTCCGGTCCC AGCCGA
//
Primer: SV40pro_F RDB16776_A8L5a.seqd.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl