Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-ChRGR-Venus

Mammalian expression vector encoding a mammalian codon-optimized chopGR.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution	Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wen, L. et al., PLoS ONE 5 (9): e12893, 2010)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的提供条件	Venus の使用について、宮脇敦史博士の書面による提供承諾を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16776_A8L5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Sequence file: RDB16776_A8L5a.seq
>D06115A8_A8L5_2_CMV_Forward_E01_13_ABI24.ab1     1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC   121 AAGCTTCGAA TTCGCCACCA TGGCTCGGAG ACCTTGGCTG CTGGCACTGG CTCTGGCTGT   181 CGCTCTGGCC GCTGGCTCTG CTGGGGCTTC TACTGGATCT GACGCAACAG TGCCCGTCGC   241 TACTCAGGAC GGACCTGATT ACGTGTTCCA CAGGGCACAT GAGCGCATGC TGTTTCAGAC   301 ATCTTATACT CTGGAAAACA ATGGCAGTGT CATCTGCATT CCCAACAATG GGCAGTGCTT   361 CTGTCTGGCA TGGCTGAAGT CTAACGGCAC CAATGCCGAG AAACTGGCCG CTAACATCCT   421 GCAGTGGATT ACATTCGCCC TGTCCGCTCT GTGCCTGATG TTTTACGGAT ATCAGACCTG   481 GAAGTCTACA TGTGGCTGGG AGGAAATCTA CGTGGCTACC ATCGAGATGA TCAAGTTCAT   541 CATTGAATAC TTCCACGAGT TTGACGAACC TGCCGTGATC TATAGCTCCA ACGGGAATAA   601 GACTGTCTGG CTGAGATACG CTGAGTGGCT GCTGACCTGT CCAGTGATCC TGATTCATCT   661 GAGCAACCTG ACTGGCCTGG CCAACGACTA TAATAAGAGA ACCATGGGGC TGCTGGTGAG   721 TGATATCGGA ACAATTGTCT GGGGCACCAC AGCAGCCCTG TCAAAAGGCT ATGTGCGCGT   781 CATCTTCTTT CTGATGGGCC TGTGCTACGG GATCTACACA TTCTTTAATG CTGCAAAGGT   841 GTACATCGAG GCATATCACA CAGTCCCTAA AGGGCGGTGT AGACAGGTGG TCACTGGAAT   901 GGCCTGGCTG TTCTTTGTGT CCTGGGGGAT GTTCCCAATC CTGTTTCTGC TGGGACCCGA   961 AAGGATTCGG CCACATTAAC CAGTTTAATT CAGCCCATCG CTCATGCAAT TCTGGACCTG  1021 GCCCAGCAAA AACTGCTGGG GGGGCTGCTT GGGACACTAC CCTGCGAGTG CTGATCACGA 1081 AGCCATATCT TGAATTCATG ACGAATAATT CGGAAGAACT TAA //
Primer: _Venus-N_R Sequence file: RDB16776_A8L5b.seq
>D06115A8_A8L5_2_Venus-N_R_F01_16_ABI24.ab1     1 CTGTCCGGGA CGCTGACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA    61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCTTCC   121 CAGTCCCTTT GTTCACGGCT CCTGCCTCGG CTTCATCTTC CACCAGTGTT TCCACCTCAA   181 TTTCGGTCCC GCCGATATTC AGTTTGGTAG TCTTCCGAAT ATCGCCATGA ATCAGGATAT   241 GCTCGTGGAT CAGCACTCGC AGGTAGTGTC CCAGCAGCCC CCAGCAGTTT TTGCTGGCCA   301 GGTCCAGAAT TGCATGAGCG ATGGCTGAAT TAAACTGGTT AATGTGGCCG AATCCTTCGG   361 GTCCCAGCAG AAACAGGATT GGGAACATCC CCCAGGACAC AAAGAACAGC CAGGCCATTC   421 CAGTGACCAC CTGTCTACAC CGCCCTTTAG GGACTGTGTG ATATGCCTCG ATGTACACCT   481 TTGCAGCATT AAAGAATGTG TAGATCCCGT AGCACAGGCC CATCAGAAAG AAGATGACGC   541 GCACATAGCC TTTTGACAGG GCTGCTGTGG TGCCCCAGAC AATTGTTCCG ATATCACTCA   601 CCAGCAGCCC CATGGTTCTC TTATTATAGT CGTTGGCCAG GCCAGTCAGG TTGCTCAGAT   661 GAATCAGGAT CACTGGACAG GTCAGCAGCC ACTCAGCGTA TCTCAGCCAG ACAGTCTTAT   721 TCCCGTTGGA GCTATAGATC ACGGCAGGTT CGTCAAACTC GTGGAAGTAT TCAATGATGA   781 ACTTGATCAT CTCGATGGTA GCCACGTAGA TTTCCTCCCA GCCACATGTA GACTTCCAGG   841 TCTGATATCC GTAAAACATC AGGCACAGAG CGGACAGGGC GAATGTAATC CACTGCAGGA   901 TGTTAGCGGC CAGTTTCTCG GCATTGGTGC CGTTAGACTT CAGCCATGCC AGACAGAAGC   961 ACTGCCCATT GTTGGGAAAT GCAGATGACA CTGCCATTGT TTTCCAGAGT ATAAGATGTC  1021 TGAAACAGCA TGCGCTCATG TGCCCTTGTT GGAACACGGT AAATCATGTT CCGATCTGAG 1081 TTAGCGGAAC GGGTAACTGA TTGCGTCAGG AATTCCAGT //
Primer: SV40polyA-R Sequence file: RDB16776_A8L5c.seq
>D06115A8_A8L5_2_SV40polyA-R_G01_19_ABI24.ab1     1 AAAAATGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA    61 GTCGCGGCCG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG   121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG   181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC   241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG   301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG   361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG   421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG   481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG   541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG   601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCCG GTGGTGCAGA TCAGCTTCAG   661 GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG CCCTCGCCCG GACACGCTGA ACTTGTGGCC   721 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC   781 CTTGCTCACC ATGGTGGCGA CCGGTGGATC CTTTCCAGTT CCTTTGTTCC ACGCTTCTTG   841 CCTCGCTTTC ATCTTTCCAC CAGTGTTTCC ACCCTCAATT TCGGTCCCGC CGAATATTCA   901 GTTTGTTAGG TCTTCGAATT ATCGCATGAA TCAGGAATAT GTCTTCGGTG GAATACAAGC   961 ACCTCGCAGT TATTGTCAGC AGCTCTCAGC AGTTTGCTTG CAAGTCGGAA TTGACTGACC 1021 AATGCCTGAT ACCTGGTTAA TTGGCCGAAT CTCCGGTCCC AGCCGA //
Primer: SV40pro_F Sequence file: RDB16776_A8L5a.seqd.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl