Sequencing and PCR primers for primer information.
Mammalian expression vector encoding a mammalian codon-optimized chopGR.
| Catalog number | RDB16776 |
|---|---|
| Resource name | pCMV-ChRGR-Venus |
| Clone info. | Mammalian expression vector encoding a mammalian codon-optimized chopGR (channelopsin/green-receiver) tagged with Venus at the C-terminus under CMV promoter. Expression was confirmed by the depositor with whole-cell patch-clamp recording. |
| Vector backbone | pVenus-N1 (plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
| Gene/insert name | Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA |
| Reference of nucleotide sequence | LC415029 (DDBJ accession number) |
| Depositor | Ishizuka, Toru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
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| Additional terms and conditions for distribution | Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16776 | pCMV-ChRGR-Venus | DNA solution |
Electronic file
Featured content
| Featured content | Genetic Resources for Optgenetics Research (English text) |
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References
| original | Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16776_A8L5p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB16776_A8L5a.seq |
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>D06115A8_A8L5_2_CMV_Forward_E01_13_ABI24.ab1 1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC 121 AAGCTTCGAA TTCGCCACCA TGGCTCGGAG ACCTTGGCTG CTGGCACTGG CTCTGGCTGT 181 CGCTCTGGCC GCTGGCTCTG CTGGGGCTTC TACTGGATCT GACGCAACAG TGCCCGTCGC 241 TACTCAGGAC GGACCTGATT ACGTGTTCCA CAGGGCACAT GAGCGCATGC TGTTTCAGAC 301 ATCTTATACT CTGGAAAACA ATGGCAGTGT CATCTGCATT CCCAACAATG GGCAGTGCTT 361 CTGTCTGGCA TGGCTGAAGT CTAACGGCAC CAATGCCGAG AAACTGGCCG CTAACATCCT 421 GCAGTGGATT ACATTCGCCC TGTCCGCTCT GTGCCTGATG TTTTACGGAT ATCAGACCTG 481 GAAGTCTACA TGTGGCTGGG AGGAAATCTA CGTGGCTACC ATCGAGATGA TCAAGTTCAT 541 CATTGAATAC TTCCACGAGT TTGACGAACC TGCCGTGATC TATAGCTCCA ACGGGAATAA 601 GACTGTCTGG CTGAGATACG CTGAGTGGCT GCTGACCTGT CCAGTGATCC TGATTCATCT 661 GAGCAACCTG ACTGGCCTGG CCAACGACTA TAATAAGAGA ACCATGGGGC TGCTGGTGAG 721 TGATATCGGA ACAATTGTCT GGGGCACCAC AGCAGCCCTG TCAAAAGGCT ATGTGCGCGT 781 CATCTTCTTT CTGATGGGCC TGTGCTACGG GATCTACACA TTCTTTAATG CTGCAAAGGT 841 GTACATCGAG GCATATCACA CAGTCCCTAA AGGGCGGTGT AGACAGGTGG TCACTGGAAT 901 GGCCTGGCTG TTCTTTGTGT CCTGGGGGAT GTTCCCAATC CTGTTTCTGC TGGGACCCGA 961 AAGGATTCGG CCACATTAAC CAGTTTAATT CAGCCCATCG CTCATGCAAT TCTGGACCTG 1021 GCCCAGCAAA AACTGCTGGG GGGGCTGCTT GGGACACTAC CCTGCGAGTG CTGATCACGA 1081 AGCCATATCT TGAATTCATG ACGAATAATT CGGAAGAACT TAA // |
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| Primer: Venus-N_R | RDB16776_A8L5b.seq |
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>D06115A8_A8L5_2_Venus-N_R_F01_16_ABI24.ab1 1 CTGTCCGGGA CGCTGACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA 61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCTTCC 121 CAGTCCCTTT GTTCACGGCT CCTGCCTCGG CTTCATCTTC CACCAGTGTT TCCACCTCAA 181 TTTCGGTCCC GCCGATATTC AGTTTGGTAG TCTTCCGAAT ATCGCCATGA ATCAGGATAT 241 GCTCGTGGAT CAGCACTCGC AGGTAGTGTC CCAGCAGCCC CCAGCAGTTT TTGCTGGCCA 301 GGTCCAGAAT TGCATGAGCG ATGGCTGAAT TAAACTGGTT AATGTGGCCG AATCCTTCGG 361 GTCCCAGCAG AAACAGGATT GGGAACATCC CCCAGGACAC AAAGAACAGC CAGGCCATTC 421 CAGTGACCAC CTGTCTACAC CGCCCTTTAG GGACTGTGTG ATATGCCTCG ATGTACACCT 481 TTGCAGCATT AAAGAATGTG TAGATCCCGT AGCACAGGCC CATCAGAAAG AAGATGACGC 541 GCACATAGCC TTTTGACAGG GCTGCTGTGG TGCCCCAGAC AATTGTTCCG ATATCACTCA 601 CCAGCAGCCC CATGGTTCTC TTATTATAGT CGTTGGCCAG GCCAGTCAGG TTGCTCAGAT 661 GAATCAGGAT CACTGGACAG GTCAGCAGCC ACTCAGCGTA TCTCAGCCAG ACAGTCTTAT 721 TCCCGTTGGA GCTATAGATC ACGGCAGGTT CGTCAAACTC GTGGAAGTAT TCAATGATGA 781 ACTTGATCAT CTCGATGGTA GCCACGTAGA TTTCCTCCCA GCCACATGTA GACTTCCAGG 841 TCTGATATCC GTAAAACATC AGGCACAGAG CGGACAGGGC GAATGTAATC CACTGCAGGA 901 TGTTAGCGGC CAGTTTCTCG GCATTGGTGC CGTTAGACTT CAGCCATGCC AGACAGAAGC 961 ACTGCCCATT GTTGGGAAAT GCAGATGACA CTGCCATTGT TTTCCAGAGT ATAAGATGTC 1021 TGAAACAGCA TGCGCTCATG TGCCCTTGTT GGAACACGGT AAATCATGTT CCGATCTGAG 1081 TTAGCGGAAC GGGTAACTGA TTGCGTCAGG AATTCCAGT // |
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| Primer: SV40polyA-R | RDB16776_A8L5c.seq |
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>D06115A8_A8L5_2_SV40polyA-R_G01_19_ABI24.ab1 1 AAAAATGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA 61 GTCGCGGCCG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 181 TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 241 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG 301 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT GTTGTAGTTG 361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC GTAGCCCAGG 601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCCG GTGGTGCAGA TCAGCTTCAG 661 GGTCAGCTTG CCGTAGTGGC ATCGCCCTCG CCCTCGCCCG GACACGCTGA ACTTGTGGCC 721 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC 781 CTTGCTCACC ATGGTGGCGA CCGGTGGATC CTTTCCAGTT CCTTTGTTCC ACGCTTCTTG 841 CCTCGCTTTC ATCTTTCCAC CAGTGTTTCC ACCCTCAATT TCGGTCCCGC CGAATATTCA 901 GTTTGTTAGG TCTTCGAATT ATCGCATGAA TCAGGAATAT GTCTTCGGTG GAATACAAGC 961 ACCTCGCAGT TATTGTCAGC AGCTCTCAGC AGTTTGCTTG CAAGTCGGAA TTGACTGACC 1021 AATGCCTGAT ACCTGGTTAA TTGGCCGAAT CTCCGGTCCC AGCCGA // |
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| Primer: SV40pro_F | RDB16776_A8L5a.seqd.seq |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. |
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2019.03.08
GNP_filter3_RDBDEP_html_190307.pl