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pCMV-chopGR-Venus

Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii.

Catalog number RDB16775
Resource name pCMV-chopGR-Venus
Alternative name ChR1-fg2
Clone info. Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
Comment Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Chlamydomonas reinhardtii acop1 (channelopsin-1) Chlamydomonas reinhardtii cop4 (acop2)

        Reference sequence
          AB058890,AF385748,AF461397,AB058891
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wen, L. et al., PLoS ONE 5 (9): e12893, 2010)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB16775 pCMV-chopGR-Venus Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The pCMV-chopGR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16775).

        Reference section:

        Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        Featured content Genetic Resources for Optgenetics Research (English text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]
        protocol Asano, T., Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells. Methods Mol. Biol. 1668: 135-145 (2017). PMID 28842907.

        2023.11.23

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