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pCMV-chopGR-Venus (#RDB16775)

Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii.

Alternative name ChR1-fg2
Clone info. Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
Comment Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions LB+Kan, 37oC
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor|Developer Ishizuka, Toru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wen, L. et al., PLoS ONE 5 (9): e12893, 2010) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wen, L. et al., PLoS ONE 5 (9): e12893, 2010)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB16775 pCMV-chopGR-Venus Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCMV-chopGR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16775).

Reference section:

Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Wen, L., Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin. PLoS One 5 (9): e12893 (2010). PMID 20886118. [link to RRC of NBRP]
protocol Asano, T., Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells. Methods Mol. Biol. 1668: 135-145 (2017). PMID 28842907.