Resource data sheet
Prev.

pCMV-ChRWR-Venus

Mammalian expression vector encoding a mammalian codon-optimized chopWR.

Catalog number RDB16774
Resource name pCMV-ChRWR-Venus
Clone info. Mammalian expression vector encoding a mammalian codon-optimized chopWR (channelopsin/wide-receiver) tagged with Venus at the C-terminus under CMV promoter.
Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor Ishizuka, Toru |
 
Other clones in our bank Chlamydomonas reinhardtii COP3 | Chlamydomonas reinhardtii COP4 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB16774 pCMV-ChRWR-Venus Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Genetic Resources for Optgenetics Research (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605.

2019.07.17

GNP_filter3_RDBDEP_html_190432.pl