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pCMV-chop2-Venus

Mammalian expression vector encoding channelopsin-2 (ChR2) from Chlamydomonas reinhardtii.

Catalog number RDB16770
Resource name pCMV-chop2-Venus
Alternative name pchop2-Venus
Clone info. Mammalian expression vector encoding channelopsin-2 (ChR2) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
Comment chop2 cDNA was a gift from Dr. Nagel (Nagel et al., Proc. Natl. Acad. Sci. USA, 100 (24): 13940, 2003). Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Chlamydomonas reinhardtii cop4 (acop2)
          Reference sequence
            AF461397,AB058891
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          RDB16770 pCMV-chop2-Venus Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCMV-chop2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16770).

          Reference section:

          Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]

          2023.11.23

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