Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGedit

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Catalog number RDB16763
Resource name pGedit
Clone info. Plasmid vector for high efficiency genome editing oligo DNA containing target sequence will be cloned into BsmBI restriction enzyme site. EGFP-blasticidin S resistant gene can be used as marker. Please refer Fig.1 of Nagasaki, A. et al. Plasmid, 98 :37-44, 2018.
Expression was confirmed by the depositor with fluorescent images. Known differences (at least): GGACGA to TCGTCC substitution corresponding to 8918 to 8923nt of LC414435.1. Nucleotide sequence of LC414435.1 will be updated.
Vector backbone pBluescript SK (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence LC414435.1 (DDBJ accession number)
Depositor Ohmiya, Yoshihiro | Nagasaki, Akira |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
Ordering Please visit Information of Request for Distribution. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (genome editing) [Word]
Schedule A (GFP-A) [Link]
Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (genome editing).
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16763 pGedit DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0099j (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16763_A8Kkp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: U6 pro,gRNA,lacO,lac pro,ori
Sequence file: RDB16763_A8Kka.seq check
>D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1
    1 AAGGCCCAGG TGAATTGTAA TACGGACTCA CTATAGGGCG AATTGGGTAC CAAGGTCGGG
   61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT
  121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA
  181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC
  241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG
  301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA
  361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA
  421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG
  481 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC
  541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
  601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
  661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
  721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
  781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
  841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
  901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
  961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
 1021 TCCTGTTCCG ACCCTGCCGC TACCGGGATA CCTGTCCGCC CTTTCTCCCT TCGGGAAGCG
 1081 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTT CGGGTGTAGT CGGTCGGCTC
1141 CAGCTGGCTG TGTGCACGAA CTCCCCCCGG TTTCAGCCCC GGAACCCGGC TG
//
Primer: CMV_forward
Region: CMV pro,insert5'(Cas9 5')
Sequence file: RDB16763_A8Kkb.seq check
>D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1
    1 GTGGTTCCAA CCCTCCCGGC CCCAATTGAC GGCAAATGGG CGGTAGGCGT GTAACGGTGG
   61 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCA TGGACAAGAA
  121 GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA
  181 CTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA
  241 GAAGAACCTG ATCGGCGCCC TGCTGTTCGG CTCTGGCGAA ACAGCCGAGG CCACCCGGCT
  301 GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA
  361 GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC
  421 CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA
  481 CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA AGCTGGCCGA
  541 CAGCACCGAC AAGGCCGACC TGAGACTGAT CTACCTGGCC CTGGCCCACA TGATCAAGTT
  601 CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT
  661 GTTCATCCAG CTGGTGCAGA TCTACAATCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG
  721 CAGAGTGGAC GCCAAGGCCA TCCTGAGCGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA
  781 TCTGATCGCC CAGCTGCCCG GCGAGAAGCG GAATGGCCTG TTCGGCAACC TGATTGCCCT
  841 GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGCA CCTGGCCGAG GATGCCAAAC
  901 TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGGC
  961 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCCTG TCCGACGCCA TCCCTGCTGA
 1021 GCGACATCCC TGAGAGTGAA CAGCGAGATC ACCCAAGGCC CCCCCCTTGT CCGGCCCTCT
 1081 ATGATTCAAG AGATACGACG AAGCACCACC AGGACCTTGA ACCCTGCCTG AAGCTCCTCG
 1141 GTGCCGGCAG GCAGCCTGGC CTGAAGAAAG TTACAAAAGA GGAATTTTTC TTTCGCCAAC
1201 CACGAG
//
Primer: pAxEF_R2
Region: SV40p(A),insert3'(Bsr,EGFP 3')
Sequence file: RDB16763_A8Kkc.seq check
>D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1
    1 CAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
   61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
  121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
  181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT
  241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT
  301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA
  361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA
  421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA
  481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT
  541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC
  601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA
  661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC
  721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC
  781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
  841 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG
  901 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC
  961 AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG
 1021 GTTCACCAGG GTGTCGCCCT TCGAACTTCA CCTCGGCGCG GGTCTTGTAG GTTGGCGTCG
 1081 TCTTTGAAGA AGATGGGTGG CGCTCTGGAC GTAGCCCTTC GGGTCAATGG ACGACCTTTG
1141 GAGAAGGTCT GCTGCTTCAT GTGGAATCCG GGAGGTTAGA AGCCCG
//
Primer: EGFP-N
Region: insert mid(EGFP5',Cas9 3')
Sequence file: RDB16763_A8Kkd.seq check
>D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1
    1 TCAAAACCGG GGGTGACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC GGTATCGATC
   61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC
  121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC
  181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG
  241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC
  301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT
  361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC
  421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA
  481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG
  541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT
  601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG
  661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA
  721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCAGCAGC TCTTTCACAC
  781 TCTTCAGTTT CTTGGACTTG CCCTTTTCCA CTTTGGCCAC CACCAGCACA GAGTAGGCCA
  841 CGGTGGGGCT GTCAAAGCCG CCGTACTTCT TAGGGTCCCA ATCCTTCTTT CTGGCGATCA
  901 GCTTGTCGGA GTTCCTCTTG GGCAGGATAG ACTCTTTGCT GAAGCCGCCG GTCTGCACCT
  961 CGGTCTTTTT TCACGATATT CACTTGGGGC ATGGACAGCA CTTTCCGCAC TGTGGCAAAA
 1021 TCCCGGGCCC TTATCCCACA CGATCTCCCC GGGTTTCGCC GTTTGTCTCG ATCAGAGCCG
 1081 CTTTTCTGAT CTCGCCGATG GGCCAGTGTG ATCTCGGGTC TTGAAGGTCC ATGAATGATG
1141 GCCTGTAGAG AAGTAACTTG GGCGGTAGCC GTTGGCCGGA A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.
reference Yamagishi, A., The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis. Int. J. Biol. Sci. 15 (7): 1546-1556 (2019). PMID 31337983.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl