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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGedit

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Catalog number RDB16763
Resource name pGedit
Clone info. Plasmid vector for high efficiency genome editing oligo DNA containing target sequence will be cloned into BsmBI restriction enzyme site. EGFP-blasticidin S resistant gene can be used as marker. Please refer Fig.1 of Nagasaki, A. et al. Plasmid, 98 :37-44, 2018.
Comment Expression was confirmed by the depositor with fluorescent images. Known differences (at least): GGACGA to TCGTCC substitution corresponding to 8918 to 8923nt of LC414435.1. Nucleotide sequence of LC414435.1 will be updated.
Vector backbone pBluescript SK (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Reference of insert sequence LC414435.1 (DDBJ accession number)
Depositor|Developer Ohmiya, Yoshihiro | Nagasaki, Akira |
Other clones in our bank

External Database
Streptococcus pyogenes Cas9 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nagasaki, A et al. Plasmid. 98, 37-44, 2018)の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16763 pGedit DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0099j (Japanese text)
          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16763_A8Kkp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: U6 pro,gRNA,lacO,lac pro,ori
          Sequence file: RDB16763_A8Kka.seq check
          >D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1
              1 AAGGCCCAGG TGAATTGTAA TACGGACTCA CTATAGGGCG AATTGGGTAC CAAGGTCGGG
             61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT
            121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA
            181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC
            241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG
            301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA
            361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA
            421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG
            481 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC
            541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
            601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
            661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
            721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
            781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
            841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
            901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
            961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
           1021 TCCTGTTCCG ACCCTGCCGC TACCGGGATA CCTGTCCGCC CTTTCTCCCT TCGGGAAGCG
           1081 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTT CGGGTGTAGT CGGTCGGCTC
          1141 CAGCTGGCTG TGTGCACGAA CTCCCCCCGG TTTCAGCCCC GGAACCCGGC TG
          //
          Primer: CMV_forward
          Region: CMV pro,insert5'(Cas9 5')
          Sequence file: RDB16763_A8Kkb.seq check
          >D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1
              1 GTGGTTCCAA CCCTCCCGGC CCCAATTGAC GGCAAATGGG CGGTAGGCGT GTAACGGTGG
             61 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCA TGGACAAGAA
            121 GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA
            181 CTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA
            241 GAAGAACCTG ATCGGCGCCC TGCTGTTCGG CTCTGGCGAA ACAGCCGAGG CCACCCGGCT
            301 GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA
            361 GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC
            421 CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA
            481 CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA AGCTGGCCGA
            541 CAGCACCGAC AAGGCCGACC TGAGACTGAT CTACCTGGCC CTGGCCCACA TGATCAAGTT
            601 CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT
            661 GTTCATCCAG CTGGTGCAGA TCTACAATCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG
            721 CAGAGTGGAC GCCAAGGCCA TCCTGAGCGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA
            781 TCTGATCGCC CAGCTGCCCG GCGAGAAGCG GAATGGCCTG TTCGGCAACC TGATTGCCCT
            841 GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGCA CCTGGCCGAG GATGCCAAAC
            901 TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGGC
            961 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCCTG TCCGACGCCA TCCCTGCTGA
           1021 GCGACATCCC TGAGAGTGAA CAGCGAGATC ACCCAAGGCC CCCCCCTTGT CCGGCCCTCT
           1081 ATGATTCAAG AGATACGACG AAGCACCACC AGGACCTTGA ACCCTGCCTG AAGCTCCTCG
           1141 GTGCCGGCAG GCAGCCTGGC CTGAAGAAAG TTACAAAAGA GGAATTTTTC TTTCGCCAAC
          1201 CACGAG
          //
          Primer: pAxEF_R2
          Region: SV40p(A),insert3'(Bsr,EGFP 3')
          Sequence file: RDB16763_A8Kkc.seq check
          >D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1
              1 CAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
             61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
            121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
            181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT
            241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT
            301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA
            361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA
            421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA
            481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT
            541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC
            601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA
            661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC
            721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC
            781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
            841 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG
            901 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC
            961 AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG
           1021 GTTCACCAGG GTGTCGCCCT TCGAACTTCA CCTCGGCGCG GGTCTTGTAG GTTGGCGTCG
           1081 TCTTTGAAGA AGATGGGTGG CGCTCTGGAC GTAGCCCTTC GGGTCAATGG ACGACCTTTG
          1141 GAGAAGGTCT GCTGCTTCAT GTGGAATCCG GGAGGTTAGA AGCCCG
          //
          Primer: EGFP-N
          Region: insert mid(EGFP5',Cas9 3')
          Sequence file: RDB16763_A8Kkd.seq check
          >D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1
              1 TCAAAACCGG GGGTGACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC GGTATCGATC
             61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC
            121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC
            181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG
            241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC
            301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT
            361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC
            421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA
            481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG
            541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT
            601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG
            661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA
            721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCAGCAGC TCTTTCACAC
            781 TCTTCAGTTT CTTGGACTTG CCCTTTTCCA CTTTGGCCAC CACCAGCACA GAGTAGGCCA
            841 CGGTGGGGCT GTCAAAGCCG CCGTACTTCT TAGGGTCCCA ATCCTTCTTT CTGGCGATCA
            901 GCTTGTCGGA GTTCCTCTTG GGCAGGATAG ACTCTTTGCT GAAGCCGCCG GTCTGCACCT
            961 CGGTCTTTTT TCACGATATT CACTTGGGGC ATGGACAGCA CTTTCCGCAC TGTGGCAAAA
           1021 TCCCGGGCCC TTATCCCACA CGATCTCCCC GGGTTTCGCC GTTTGTCTCG ATCAGAGCCG
           1081 CTTTTCTGAT CTCGCCGATG GGCCAGTGTG ATCTCGGGTC TTGAAGGTCC ATGAATGATG
          1141 GCCTGTAGAG AAGTAACTTG GGCGGTAGCC GTTGGCCGGA A
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.
          reference Zhao, J., Development of aptamer-based inhibitors for CRISPR/Cas system. Nucleic Acids Res. 49 (3): 1330-1344 (2021). PMID 33123724.
          reference Yamagishi, A., The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis. Int. J. Biol. Sci. 15 (7): 1546-1556 (2019). PMID 31337983.

          2022.05.17

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