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pGedit

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Catalog number RDB16763
Resource name pGedit
Clone info. Plasmid vector for high efficiency genome editing oligo DNA containing target sequence will be cloned into BsmBI restriction enzyme site. EGFP-blasticidin S resistant gene can be used as marker. Please refer Fig.1 of Nagasaki, A. et al. Plasmid, 98 :37-44, 2018.
Comment Expression was confirmed by the depositor with fluorescent images. Known differences (at least): GGACGA to TCGTCC substitution corresponding to 8918 to 8923nt of LC414435.1. Nucleotide sequence of LC414435.1 will be updated.
Vector backbone pBluescript SK (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Reference of insert sequence LC414435.1 (DDBJ accession number)
Depositor|Developer Ohmiya, Yoshihiro | Nagasaki, Akira |
Other clones in our bank

External Database
Streptococcus pyogenes Cas9 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nagasaki, A et al. Plasmid. 98, 37-44, 2018)の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16763 pGedit DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGedit was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16763).

          Reference section:

          Nagasaki, A., Kato, Y., Meguro, K., Yamagishi, A., Nakamura, C., Uyeda, T.Q.P., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0099j (Japanese text)
          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16763_A8Kkp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 (Pr0410)
          Region: T7 pro,U6 pro,gRDA scaffold,T3 pro
          Sequence file: RDB16763_A8Kke.seq check
          >D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNANTNN CTATAGGGCG AATTGGGTAC CAAGGTCGGG
             61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT
            121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA
            181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC
            241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG
            301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA
            361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA
            421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG
            481 CTTGGCGTAA TCANGNNCAT NNCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC
            541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
            601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
            661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
            721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
            781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCANGG GATAACGCAN GAAAGAACAT
            841 GTGAGCAAAA GGCCAGCNAA AGGNCAGGAA NCGTAAAAAN GCCGCGTTGC TGGCGTTTTT
            901 CCNTANGCNN CGCCCCCNNG ANNANCATCN CAAAAATCNA CGCTCNAGTC ANANGNGGNN
            961 AANNCCGACN NGANTATAAA GANNCCANNN GTTTCCNNNT NNAANCNCCC NNNNGCNNTN
          1021 NNNNGNNNNN ANNNNNNNNN N
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,Cas9 5'
          Sequence file: RDB16763_A8Kkf.seq check
          >D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNATGGG CGGTAGGCGT GTANCGGTGG
             61 GAGGNNNNNN NAAGCANNAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC ATGGACAAGA
            121 AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG
            181 ACTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA
            241 AGAAGAACCT GATCGGCGCC CTGCTGTTCG GCTCTGGCGA AACAGCCGAG GCCACCCGGC
            301 TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG
            361 AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT
            421 CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG
            481 ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAGCTGGCCG
            541 ACAGCACCGA CAAGGCCGAC CTGAGACTGA TCTACCTGGC CCTGGCCCAC ATGATCAAGT
            601 TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC
            661 TGTTCATCCA GCTGGTGCAG ATCTACAATC AGCTGTTCGA GGAAAACCCC ATCAACGCCA
            721 GCAGAGTGGA CGCCAANGCC ATCCTGAGCG CCNNACTGAG CAAGAGCAGA CGGCTGGAAA
            781 ATCTGATCGC CCANCTGCCC GGCGAGAAGC GGAATGGCCT GTNCGGCNNC CTGATTGCCC
            841 TGANCCTGGG CCNGANCCCC AACTTCNNNA GCAACTTCNN NNNTGNNNGA NGANGCCNAA
            901 CTGCAGCTGA NCNNGGACAC CTACGACGAN NACCTGNNNN NNCTGCTGGC CCNNATCNGN
            961 NGANCANNAC NNCGACCTGT TTCNNGNCNN CNAGAANCNT GTNNNANNNN NNNCNNGCNN
          1021 ANCNNCNTNC NNNANNNNNN NNNN
          //
          Primer: EGFP-N (Pr0027)
          Region: T2A,SV40 NLS,Cas9 3'
          Sequence file: RDB16763_A8Kkg.seq check
          >D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTCGCCCT TGCTNNCNNT GGTGGCGANC GGTATCGATC
             61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC
            121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC
            181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG
            241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC
            301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT
            361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC
            421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA
            481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG
            541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT
            601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG
            661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA
            721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCANCAGC TCTTTCACAC
            781 TCTTCAGTTN CTTGGACTTG CCCTTTTCCN CTTTGGCCAC CACCAGCACA GANTANGCCA
            841 CGGNGGGNNT GTCAAAGCCG CCGTACTTCT TANGGNNCCN ATCCTTCTTT CTGGNNATCA
            901 GCTTGNCNNA NTNNCTCTNN GGCAGGANAN NNTCTTNGCT GAANNCGNNN GNCTGNNNNN
          961 CGNNCTTTTT NNACNANATT CNCTTGGGGN ANGGNNAGCN NTTTNNNNNN NNNN
          //
          Primer: pAxEF R2 (Pr0059)
          Region: SV40 pA,Bsr,EGFP 3'
          Sequence file: RDB16763_A8Kkh.seq check
          >D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNGNTTNNNT TNNGANNTTG TGANGCTATT GCTTTATTTG TAACCATTAT
             61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
            121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
            181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT
            241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT
            301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA
            361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA
            421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA
            481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT
            541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC
            601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA
            661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC
            721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC
            781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
            841 TGGTCGGCGA NCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAN NTTCACCTTG
            901 ATGNCGTTCT TCNGCTTGNC NGNCATGANA TAGANGTTGT NNNTGTTGTA NTTGTACTCC
            961 AGCTTGNGCC CCNNGANGTT GNCGNCCNNC NNNANNNNNA NGNCNNTCNG CNNNANGCNG
          1021 NNNCANCNNG NNNNNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: CMV pro,Cas9 5'
          Sequence file: RDB16763_A8Kki.seq check
          >16763_16763_B2G6F_Amp_R_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNANNNN NNNNNNNGGG TTATTGTCTC NTGAGCGGAN
             61 ACATATGCCA AGTACGCCCC CNATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA
            121 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT
            181 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA
            241 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA
            301 AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG
            361 TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG
            421 CTAGCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG
            481 CCGTGATCAC CGACGACTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG
            541 ACCGGCACAG CATCAAGAAG AACCTGATCG GCGCCCTGCT GTTCGGCTCT GGCGAAACAG
            601 CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA
            661 TCTGCTATCT GCAAGAGATC TTCNGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC
            721 ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CNCCCCATCT
            781 TCNGCAACAT CGTGGACGAN GTGGCCTANC ACGAGAANNT ANCCCACCAT CTACNACCTG
            841 ANAAAGAAGC TGGCCGACAG CANCGACAAN GNCCGACCTG AGACTGATCT ACNNGGNCCN
            901 GGCCCANATG ATCNAGTTCC GGGGCCACTT CCTGNTCGAG GGCGANCNTG AACCCCNACN
            961 NCNNCNANNN NGNANNANCT GTNCATNCNG CTNGGTGCNN NNCTACNANT NNNNNTNTNC
          1021 NANGNAAAAC CNNNNTNNNN NNNNNN
          //
          Primer: FP-N_F1 (Pr0920)
          Region: EGFP 3',Bsr 5'
          Sequence file: RDB16763_A8Kkj.seq check
          >16763_16763_B2G6F_FP-N_F1_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNN NNNNNNCNTG NAGCAGCACG ACTTCTTCNA GTCCGCCATG
             61 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC
            121 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC
            181 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC
            241 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC
            301 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC
            361 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC
            421 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG
            481 ATCACTCTCG GCATGGACGA GCTGTACAAG TACTCAGATC CAATGAAAAC ATTTAACATT
            541 TCTCAACAGG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
            601 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
            661 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
            721 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
            781 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
            841 ATTTCAGACT ATGCACNAGA TTGTTTTGTG TTAATAGAAA TGNNTGGNAA GTTAGTCAAA
            901 ACTACGANTG ANNAACTCAT TNNNCTCNAA TATANCCGAA ANTNANGNAN NCNNNCNNAC
            961 CGCGGGCCCG GGNNNCNNNN GNATCNAGAN NNCTGNTNAN NANTCNGCCN NNNNNNNNTN
          1021 NNNNNNGNNT TTNNNNNNNN TNNAAAANNN NNNNNNN
          //
          Primer: EGFP-M_R (Pr0743)
          Region: EGFP 5',T2A,SV40 NLS,Cas9 3'
          Sequence file: RDB16763_A8Kkk.seq check
          >D05970A1_A8Kk_2_EGFP-M_R_F08_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNC TTGNNGTTCN CCTTGNNGCC GTTCTTCTGC
             61 TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG
            121 ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG
            181 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG
            241 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG
            301 TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG
            361 GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTAGGTGGCA
            421 TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG
            481 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC
            541 GGTATCGATC GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG
            601 CCCTCTCCAC TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC
            661 AGGTCGATCC GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC
            721 ACCTCTTTGG TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCNAAGTA CTTGAANGCG
            781 GCAGGGGCTC NCAGGTTGGT CAGGGTAAAC ANGTGGATGA TATTCTCGGN CTGCTCTCTG
            841 ATGGGCTTAT CCCNGGNGCT TGTTGTANGC GCTCANCACC TTGTCNNGGT TGGCATCGGC
            901 CANGATCNNG CGCTTGGANA NNTCNCTAAT CTGNTCNATG ATNTCGTNNN GNNNGNGCTT
            961 GTGCTGNTNC NCNAACAGCT GTTTCNGCTN ATTATNCNNN GGGGGANNCN NTNNNNCTNN
          1021 NCNNNNNNGN NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057.
          reference Zhao, J., Development of aptamer-based inhibitors for CRISPR/Cas system. Nucleic Acids Res. 49 (3): 1330-1344 (2021). PMID 33123724.
          reference Yamagishi, A., The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis. Int. J. Biol. Sci. 15 (7): 1546-1556 (2019). PMID 31337983.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl