RDB16763 - pGedit

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pGedit (#RDB16763)

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Clone info.	Plasmid vector for high efficiency genome editing oligo DNA containing target sequence will be cloned into BsmBI restriction enzyme site. EGFP-blasticidin S resistant gene can be used as marker. Please refer Fig.1 of Nagasaki, A. et al. Plasmid, 98 :37-44, 2018.
Comment	Expression was confirmed by the depositor with fluorescent images. Known differences (at least): GGACGA to TCGTCC substitution corresponding to 8918 to 8923nt of LC414435.1. Nucleotide sequence of LC414435.1 will be updated.
Vector backbone	pBluescript SK (Plasmid)
Selectable markers	Amp^r
Growth conditions	LB+Ampicillin, 37oC
Gene/insert name	Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Reference of insert sequence	LC414435.1 (DDBJ accession number)
Depositor\|Developer	Ohmiya, Yoshihiro \| Nagasaki, Akira \|

Sequence (full)	RDB16763hts01.seq Assembled from experimentally sequenced data.
Publication	Nagasaki, A., Plasmid 98: 37-44 (2018). [link to RRC of NBRP]
Test sheet	RDB16763B0p1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献（Nagasaki, A et al. Plasmid. 98, 37-44, 2018）の引用を必要とする。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16763	pGedit	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGedit was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16763).

Reference section:

Nagasaki, A., Kato, Y., Meguro, K., Yamagishi, A., Nakamura, C., Uyeda, T.Q.P., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057. [link to RRC of NBRP]
reference	Zhao, J., Development of aptamer-based inhibitors for CRISPR/Cas system. Nucleic Acids Res. 49 (3): 1330-1344 (2021). PMID 33123724.
reference	Yamagishi, A., The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis. Int. J. Biol. Sci. 15 (7): 1546-1556 (2019). PMID 31337983.