Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGedit

Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Nagasaki, A et al. Plasmid. 98, 37-44, 2018）の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16763_A8Kkp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: U6 pro,gRNA,lacO,lac pro,ori Sequence file: RDB16763_A8Kka.seq
>D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1     1 AAGGCCCAGG TGAATTGTAA TACGGACTCA CTATAGGGCG AATTGGGTAC CAAGGTCGGG    61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT   121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA   181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC   241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG   301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA   361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA   421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG   481 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC   541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA   601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA   661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC   721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC   781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT   841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT   901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG   961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC  1021 TCCTGTTCCG ACCCTGCCGC TACCGGGATA CCTGTCCGCC CTTTCTCCCT TCGGGAAGCG  1081 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTT CGGGTGTAGT CGGTCGGCTC 1141 CAGCTGGCTG TGTGCACGAA CTCCCCCCGG TTTCAGCCCC GGAACCCGGC TG //
Primer: CMV_forward Region: CMV pro,insert5'(Cas9 5') Sequence file: RDB16763_A8Kkb.seq
>D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1     1 GTGGTTCCAA CCCTCCCGGC CCCAATTGAC GGCAAATGGG CGGTAGGCGT GTAACGGTGG    61 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCA TGGACAAGAA   121 GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA   181 CTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA   241 GAAGAACCTG ATCGGCGCCC TGCTGTTCGG CTCTGGCGAA ACAGCCGAGG CCACCCGGCT   301 GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA   361 GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC   421 CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA   481 CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA AGCTGGCCGA   541 CAGCACCGAC AAGGCCGACC TGAGACTGAT CTACCTGGCC CTGGCCCACA TGATCAAGTT   601 CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT   661 GTTCATCCAG CTGGTGCAGA TCTACAATCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG   721 CAGAGTGGAC GCCAAGGCCA TCCTGAGCGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA   781 TCTGATCGCC CAGCTGCCCG GCGAGAAGCG GAATGGCCTG TTCGGCAACC TGATTGCCCT   841 GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGCA CCTGGCCGAG GATGCCAAAC   901 TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGGC   961 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCCTG TCCGACGCCA TCCCTGCTGA  1021 GCGACATCCC TGAGAGTGAA CAGCGAGATC ACCCAAGGCC CCCCCCTTGT CCGGCCCTCT  1081 ATGATTCAAG AGATACGACG AAGCACCACC AGGACCTTGA ACCCTGCCTG AAGCTCCTCG  1141 GTGCCGGCAG GCAGCCTGGC CTGAAGAAAG TTACAAAAGA GGAATTTTTC TTTCGCCAAC 1201 CACGAG //
Primer: pAxEF_R2 Region: SV40p(A),insert3'(Bsr,EGFP 3') Sequence file: RDB16763_A8Kkc.seq
>D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1     1 CAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT    61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG   121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA   181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT   241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT   301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA   361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA   421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA   481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT   541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC   601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA   661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC   721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC   781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG   841 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG   901 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC   961 AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG  1021 GTTCACCAGG GTGTCGCCCT TCGAACTTCA CCTCGGCGCG GGTCTTGTAG GTTGGCGTCG  1081 TCTTTGAAGA AGATGGGTGG CGCTCTGGAC GTAGCCCTTC GGGTCAATGG ACGACCTTTG 1141 GAGAAGGTCT GCTGCTTCAT GTGGAATCCG GGAGGTTAGA AGCCCG //
Primer: EGFP-N Region: insert mid(EGFP5',Cas9 3') Sequence file: RDB16763_A8Kkd.seq
>D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1     1 TCAAAACCGG GGGTGACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC GGTATCGATC    61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC   121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC   181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG   241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC   301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT   361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC   421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA   481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG   541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT   601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG   661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA   721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCAGCAGC TCTTTCACAC   781 TCTTCAGTTT CTTGGACTTG CCCTTTTCCA CTTTGGCCAC CACCAGCACA GAGTAGGCCA   841 CGGTGGGGCT GTCAAAGCCG CCGTACTTCT TAGGGTCCCA ATCCTTCTTT CTGGCGATCA   901 GCTTGTCGGA GTTCCTCTTG GGCAGGATAG ACTCTTTGCT GAAGCCGCCG GTCTGCACCT   961 CGGTCTTTTT TCACGATATT CACTTGGGGC ATGGACAGCA CTTTCCGCAC TGTGGCAAAA  1021 TCCCGGGCCC TTATCCCACA CGATCTCCCC GGGTTTCGCC GTTTGTCTCG ATCAGAGCCG  1081 CTTTTCTGAT CTCGCCGATG GGCCAGTGTG ATCTCGGGTC TTGAAGGTCC ATGAATGATG 1141 GCCTGTAGAG AAGTAACTTG GGCGGTAGCC GTTGGCCGGA A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl