Sequencing and PCR primers for primer information.
Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.
| Catalog number | RDB16763 |
|---|---|
| Resource name | pGedit |
| Clone info. | Plasmid vector for high efficiency genome editing oligo DNA containing target sequence will be cloned into BsmBI restriction enzyme site. EGFP-blasticidin S resistant gene can be used as marker. Please refer Fig.1 of Nagasaki, A. et al. Plasmid, 98 :37-44, 2018. Expression was confirmed by the depositor with fluorescent images. Known differences (at least): GGACGA to TCGTCC substitution corresponding to 8918 to 8923nt of LC414435.1. Nucleotide sequence of LC414435.1 will be updated. |
| Vector backbone | pBluescript SK (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA |
| Reference of nucleotide sequence | LC414435.1 (DDBJ accession number) |
| Depositor | Ohmiya, Yoshihiro | Nagasaki, Akira | |
| Other clones in our bank | Streptococcus pyogenes (KEGG orthology K09952) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagasaki, A et al. Plasmid. 98, 37-44, 2018) is requested. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA (genome editing) [Word] Schedule A (GFP-A) [Link] |
| Remarks | The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (genome editing). Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16763 | pGedit | DNA solution |
Electronic file
Original reference
| original | Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0099j (Japanese text) |
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| Featured content | Genome Editing (English text) |
| Featured content | Genome Editing (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16763_A8Kkp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | Sequence: RDB16763_A8Kka.seq |
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>D05970A1_A8Kk_2_M13_-40_A04_01_ABI08.ab1 1 AAGGCCCAGG TGAATTGTAA TACGGACTCA CTATAGGGCG AATTGGGTAC CAAGGTCGGG 61 CAGGAAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT 121 AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA 181 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC 241 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 301 AAAGGACGAA ACACCGGAGA CGAATTCGTC TCTTTAAGAG CTAGAAATAG CAAGTTTAAA 361 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTCTAGA 421 GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG 481 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 541 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 601 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 1021 TCCTGTTCCG ACCCTGCCGC TACCGGGATA CCTGTCCGCC CTTTCTCCCT TCGGGAAGCG 1081 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTT CGGGTGTAGT CGGTCGGCTC 1141 CAGCTGGCTG TGTGCACGAA CTCCCCCCGG TTTCAGCCCC GGAACCCGGC TG // |
| Primer: CMV_Forward | Sequence: RDB16763_A8Kkb.seq |
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>D05970A1_A8Kk_2_CMV Forward_B04_02_ABI08.ab1 1 GTGGTTCCAA CCCTCCCGGC CCCAATTGAC GGCAAATGGG CGGTAGGCGT GTAACGGTGG 61 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCA TGGACAAGAA 121 GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA 181 CTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA 241 GAAGAACCTG ATCGGCGCCC TGCTGTTCGG CTCTGGCGAA ACAGCCGAGG CCACCCGGCT 301 GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA 361 GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC 421 CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA 481 CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC CTGAGAAAGA AGCTGGCCGA 541 CAGCACCGAC AAGGCCGACC TGAGACTGAT CTACCTGGCC CTGGCCCACA TGATCAAGTT 601 CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT 661 GTTCATCCAG CTGGTGCAGA TCTACAATCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG 721 CAGAGTGGAC GCCAAGGCCA TCCTGAGCGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA 781 TCTGATCGCC CAGCTGCCCG GCGAGAAGCG GAATGGCCTG TTCGGCAACC TGATTGCCCT 841 GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGCA CCTGGCCGAG GATGCCAAAC 901 TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGGC 961 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCCTG TCCGACGCCA TCCCTGCTGA 1021 GCGACATCCC TGAGAGTGAA CAGCGAGATC ACCCAAGGCC CCCCCCTTGT CCGGCCCTCT 1081 ATGATTCAAG AGATACGACG AAGCACCACC AGGACCTTGA ACCCTGCCTG AAGCTCCTCG 1141 GTGCCGGCAG GCAGCCTGGC CTGAAGAAAG TTACAAAAGA GGAATTTTTC TTTCGCCAAC 1201 CACGAG // |
| Primer: pAxEF R2 | Sequence: RDB16763_A8Kkc.seq |
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>D05970A1_A8Kk_2_pAxEF-R2_D03_12_ABI24.ab1 1 CAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT 61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG 121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA 181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACGGACGAGT ACCTTAATTT 241 CGGGTATATT TGAGTGGAAT GAGTTCTTCA ATCGTAGTTT TGACTAACTT GCCATTCATT 301 TCTATTAACA CAAAACAATC TGGTGCATAG TCTGAAATCA ACTCCCTACA CATACCACAA 361 GGACTTACCA CTCGAATACT TCTATCTACT TCGTCAGAAT AAGGGTGTCT AACAGCTACA 421 ATCGTGTCAA AATCCTTTTG TCCATTCGAA ACTGCACTAC CAATCGCAAT GGCTTCTGCA 481 CAAACAGTTA CTCGTCCTAT ATACGCTTCA ATATGTACTG CCGAAATGAT TTCTCCTGTT 541 TTCGTACGAA TTGCCGCTCC CACATGATGT TTATTATCCT CATAAAGCAT TGTAATCTTC 601 TCTGTCGCTA CTTCTACTAA TTCTAGATCC TGTTGAGAAA TGTTAAATGT TTTCATTGGA 661 TCTGAGTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC 721 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC 781 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG 841 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG 901 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC 961 AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG 1021 GTTCACCAGG GTGTCGCCCT TCGAACTTCA CCTCGGCGCG GGTCTTGTAG GTTGGCGTCG 1081 TCTTTGAAGA AGATGGGTGG CGCTCTGGAC GTAGCCCTTC GGGTCAATGG ACGACCTTTG 1141 GAGAAGGTCT GCTGCTTCAT GTGGAATCCG GGAGGTTAGA AGCCCG // |
| Primer: EGFP-N | Sequence: RDB16763_A8Kkd.seq |
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>D05970A1_A8Kk_2_EGFP-N_D04_04_ABI08.ab1 1 TCAAAACCGG GGGTGACAGC TCCTCGCCCT TGCTCACCAT GGTGGCGACC GGTATCGATC 61 GTGGGCCAGG ATTCTCCTCG ACGTCACCGC ATGTTAGCAG ACTTCCTCTG CCCTCTCCAC 121 TGCCGTTAAC CACTTTGCGC TTTTTCTTGG GGTCGCCTCC CAGCTGAGAC AGGTCGATCC 181 GTGTCTCGTA CAGGCCGGTG ATGCTCTGGT GGATCAGGGT GGCGTCCAGC ACCTCTTTGG 241 TGCTGGTGTA CCTCTTCCGG TCGATGGTGG TGTCAAAGTA CTTGAAGGCG GCAGGGGCTC 301 CCAGGTTGGT CAGGGTAAAC AGGTGGATGA TATTCTCGGC CTGCTCTCTG ATGGGCTTAT 361 CCCGGTGCTT GTTGTAGGCG CTCAGCACCT TGTCCAGGTT GGCATCGGCC AGGATCACGC 421 GCTTGGAGAA CTCGCTAATC TGCTCGATGA TCTCGTCCAG GTAGTGCTTG TGCTGTTCCA 481 CAAACAGCTG TTTCTGCTCA TTATCCTCGG GGGAGCCCTT CAGCTTCTCA TAGTGGCTGG 541 CCAGGTACAG GAAGTTCACA TATTTGGAGG GCAGGGCCAG CTCGTTTCCC TTCTGCAGTT 601 CGCCGGCAGA AGCCAGCATC CGCTTCCGGC CGTTTTCCAG CTCGAACAGG GAGTACTTAG 661 GCAGCTTGAT GATCAGGTCC TTTTTCACTT CTTTGTAGCC CTTGGCTTCC AGAAAGTCGA 721 TGGGATTCTT CTCGAAGCTG CTTCTTTCCA TGATGGTGAT CCCCAGCAGC TCTTTCACAC 781 TCTTCAGTTT CTTGGACTTG CCCTTTTCCA CTTTGGCCAC CACCAGCACA GAGTAGGCCA 841 CGGTGGGGCT GTCAAAGCCG CCGTACTTCT TAGGGTCCCA ATCCTTCTTT CTGGCGATCA 901 GCTTGTCGGA GTTCCTCTTG GGCAGGATAG ACTCTTTGCT GAAGCCGCCG GTCTGCACCT 961 CGGTCTTTTT TCACGATATT CACTTGGGGC ATGGACAGCA CTTTCCGCAC TGTGGCAAAA 1021 TCCCGGGCCC TTATCCCACA CGATCTCCCC GGGTTTCGCC GTTTGTCTCG ATCAGAGCCG 1081 CTTTTCTGAT CTCGCCGATG GGCCAGTGTG ATCTCGGGTC TTGAAGGTCC ATGAATGATG 1141 GCCTGTAGAG AAGTAACTTG GGCGGTAGCC GTTGGCCGGA A // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Nagasaki, A., A genome editing vector that enables easy selection and identification of knockout cells. Plasmid 98: 37-44 (2018). PMID 30196057. |
|---|
2019.09.19
GNP_filter3_RDBDEP_html_190816.pl