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pcDNA-Ostir1-pA (#RDB16762)

Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR).

Clone info. Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR) mRNA synthesis. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence.
Comment Expression was confirmed by the depositor with EGFP monitoring by microinjectioning its mRNA with hPLCz-AID-EGFP mRNA into mouse oocytes and culturing them in the presence of indole-3-acetic acid.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Ogura, Atsuo |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) is requested.
Additional terms and conditions:
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018） の引用を必要とする。
MTAに書く付加的使用条件：
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16762 pcDNA-Ostir1-pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA-Ostir1-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16762).

Reference section:

Miura, K., Matoba, S., Ogonuki, N., Namiki, T., Ito, J., Kashiwazaki, N., Ogura, A., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16762_A8K6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB16762_A8K6a.seq check
>D06043A2_A8K6_2_CMV-Forward_E06_15_ABI24.ab1
    1 AGGTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTCG AATTATGACG TACTTCCCGG
  181 AGGAGGTGGT GGAGCACATC TTCAGCTTCC TGCCGGCGCA GCGCGACCGC AACACGGTCT
  241 CGCTCGTCTG CAAGGTGTGG TACGAGATCG AGAGGCTGAG CCGCCGCGGC GTCTTCGTGG
  301 GCAACTGCTA CGCCGTGCGC GCCGGCCGCG TCGCCGCGCG GTTCCCCAAC GTGCGGGCGC
  361 TCACGGTGAA GGGGAAGCCC CACTTCGCCG ACTTCAACCT CGTGCCCCCC GACTGGGGCG
  421 GCTACGCGGG GCCGTGGATC GAGGCGGCCG CGAGGGGATG CCACGGCCTG GAGGAGCTCA
  481 GGATGAAGCG GATGGTGGTG TCCGACGAGA GCCTCGAGCT GCTGGCTCGC TCGTTCCCGC
  541 GGTTCAGGGC TCTTGTTCTT ATCAGCTGCG AGGGGTTCAG CACTGACGGG CTAGCCGCCG
  601 TCGCGAGCCA TTGCAAGCTT CTGAGGGAGT TGGATTTGCA GGAAAATGAA GTGGAGGATC
  661 GAGGGCCTAG TGGCTTTCCT GCTTCCCTGA TTCCTGCACA TCACTGTCTC ATGATTTTGC
  721 CTGCATCAAA GGGGAGGTAT GCTGTCCACT GGAGAGACTT GTTAGCAGGT CCCCAAACTG
  781 GCGGAGTTTG AGGCTGATCG ATCTTGTATC GTAGAATACA CTTGCAAGAT ACTACTGCGT
  841 ACCCCTACTG GAGGATTGGG GAATGGGTAT TTGGACGGAT GACTTCAACC TGAATCCTCA
  901 TTTAAGCTAC CAGGCCTGGA ATGCCAATGT GAGATGCTGG AATCCGGACC CTCCGATGCC
961 GTCATATATA C
//
Primer: GNP_Primer#10 ()
Sequence file: RDB16762_A8K6b.seq check
>D06043A2_A8K6_2_GNP_Primer#10_F06_18_ABI24.ab1
    1 TAATTTGTGC AAATTCAATT TAAAAGATAA CGTTTACAGG TTCTTTGGTG CCCATAGTCG
   61 AGCGGCCTTA ACCACTAGCA GCAGAACCGG AGTCGACTAG GATTTTAACA AAATTTGGTG
  121 CATCATCCCT CGCCCCAGCA GTTGTGCGGT ACACATATAA TTTCTCCACT TTAGGCAGAT
  181 CTCCATGGTT TTCCTCCATT TCATTGCTAC CATCCCGCTC ATTTATGACC TCAACATTGA
  241 GCATCGGCAT CTTTGACGCA AGGACTTGGC ACCCCTTTAA CGTGACATTG CAAGATGACA
  301 TCCAAAGGGA TCGCATTGTC TCGTACCTAG CAAAATTCCC CAAGAGTGCA GCATCACCAA
  361 ACGGGCTATC TCTTATCTCC AGTTTCCTTA AATTCTTGCA TCCATTCATA ACATGCATCA
  421 TACCCTTATC ACTGTCACCA GCAAATGCTA TAGAAAGCAT CTCAAGTTGT TTTGCATATT
  481 TCCCAATATA CATGAAAACT TTGTCTGTGA GAAGACCAGA TATTGACAAA CGTTGTAATC
  541 CCTTGCACTC ACGAACAATA GCTCCAAAGC CTTCATCTAA TGGTTGGCTT GTCACAACAT
  601 CAGGCTTCCC TGGCTCAAGA ATACAAAGTC TGAATCGTGT GAAATTTGGA CAGTTCTTGG
  661 CGACAGTAAC TAGTGCAGCA TTAGTCATTT GGTGACAGAA GTACAGTAGT GAGTTCAGTT
  721 TTGGACAGCC CAAGGATACT GCAACAAGTC CCTCCTCTGT CACTGCAGAA TAACCAGCTA
  781 CGTAGAAATC TGATGGAAAT ACCCTGAGTT CTTGCAAGTC TTTGCAACTG GAGGCCACCA
  841 CTTGCAAGCC TTTGTCCGAG ATACAATCCA GTACCCAAAG GCGTTGGAGC TTCACACAGC
  901 GGCTAATCAT TTTTTGTAAG GTCAGAAGGC ATCAAAGTGT GGGGTGCATA GCCTCAAGTT
  961 CAATCCTGTC AGTTGAGCAA CACAAGGGGG GTAGAATAAA TGACAGGCAA ACCAGGAGAA
 1021 GCATCCAGAA TCAGACAACT CCTCAACCAT CCTGGCATTT CTCAGAGACA CTTGGTAGCC
1081 TTTAAAGTTA GGACTCAGTT GGAAGTCAAT CCTGG
//
Primer: SV40pro_F ()
Sequence file: RDB16762_A8K6c.seq check
>D06043A2_A8K6_2_SV40pro_F_G06_21_ABI24.ab1
    1 CCTGGTCCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAAGC
  961 GACGCCCAAC CCTGCCCATC ACGAGATTTC GATTTCCACC GCCGCCTTCT ATGAAGGTTG
 1021 GGCTTTCGGA ATCGGTTTTC CGGGACGCCG CTGGAATGGA TTCTTCAAGC GCGAGCATCT
1081 CATGCTGGAA GCTCATCGCC CAACCCCAAC CTTTGGTCCA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. [link to RRC of NBRP]