Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA-Ostir1-pA

Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) is requested.
Additional terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018） の引用を必要とする。
付加的提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16762_A8K6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB16762_A8K6a.seq
>D06043A2_A8K6_2_CMV-Forward_E06_15_ABI24.ab1     1 AGGTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTCG AATTATGACG TACTTCCCGG   181 AGGAGGTGGT GGAGCACATC TTCAGCTTCC TGCCGGCGCA GCGCGACCGC AACACGGTCT   241 CGCTCGTCTG CAAGGTGTGG TACGAGATCG AGAGGCTGAG CCGCCGCGGC GTCTTCGTGG   301 GCAACTGCTA CGCCGTGCGC GCCGGCCGCG TCGCCGCGCG GTTCCCCAAC GTGCGGGCGC   361 TCACGGTGAA GGGGAAGCCC CACTTCGCCG ACTTCAACCT CGTGCCCCCC GACTGGGGCG   421 GCTACGCGGG GCCGTGGATC GAGGCGGCCG CGAGGGGATG CCACGGCCTG GAGGAGCTCA   481 GGATGAAGCG GATGGTGGTG TCCGACGAGA GCCTCGAGCT GCTGGCTCGC TCGTTCCCGC   541 GGTTCAGGGC TCTTGTTCTT ATCAGCTGCG AGGGGTTCAG CACTGACGGG CTAGCCGCCG   601 TCGCGAGCCA TTGCAAGCTT CTGAGGGAGT TGGATTTGCA GGAAAATGAA GTGGAGGATC   661 GAGGGCCTAG TGGCTTTCCT GCTTCCCTGA TTCCTGCACA TCACTGTCTC ATGATTTTGC   721 CTGCATCAAA GGGGAGGTAT GCTGTCCACT GGAGAGACTT GTTAGCAGGT CCCCAAACTG   781 GCGGAGTTTG AGGCTGATCG ATCTTGTATC GTAGAATACA CTTGCAAGAT ACTACTGCGT   841 ACCCCTACTG GAGGATTGGG GAATGGGTAT TTGGACGGAT GACTTCAACC TGAATCCTCA   901 TTTAAGCTAC CAGGCCTGGA ATGCCAATGT GAGATGCTGG AATCCGGACC CTCCGATGCC 961 GTCATATATA C //
Primer: GNP_Primer#10 Sequence file: RDB16762_A8K6b.seq
>D06043A2_A8K6_2_GNP_Primer#10_F06_18_ABI24.ab1     1 TAATTTGTGC AAATTCAATT TAAAAGATAA CGTTTACAGG TTCTTTGGTG CCCATAGTCG    61 AGCGGCCTTA ACCACTAGCA GCAGAACCGG AGTCGACTAG GATTTTAACA AAATTTGGTG   121 CATCATCCCT CGCCCCAGCA GTTGTGCGGT ACACATATAA TTTCTCCACT TTAGGCAGAT   181 CTCCATGGTT TTCCTCCATT TCATTGCTAC CATCCCGCTC ATTTATGACC TCAACATTGA   241 GCATCGGCAT CTTTGACGCA AGGACTTGGC ACCCCTTTAA CGTGACATTG CAAGATGACA   301 TCCAAAGGGA TCGCATTGTC TCGTACCTAG CAAAATTCCC CAAGAGTGCA GCATCACCAA   361 ACGGGCTATC TCTTATCTCC AGTTTCCTTA AATTCTTGCA TCCATTCATA ACATGCATCA   421 TACCCTTATC ACTGTCACCA GCAAATGCTA TAGAAAGCAT CTCAAGTTGT TTTGCATATT   481 TCCCAATATA CATGAAAACT TTGTCTGTGA GAAGACCAGA TATTGACAAA CGTTGTAATC   541 CCTTGCACTC ACGAACAATA GCTCCAAAGC CTTCATCTAA TGGTTGGCTT GTCACAACAT   601 CAGGCTTCCC TGGCTCAAGA ATACAAAGTC TGAATCGTGT GAAATTTGGA CAGTTCTTGG   661 CGACAGTAAC TAGTGCAGCA TTAGTCATTT GGTGACAGAA GTACAGTAGT GAGTTCAGTT   721 TTGGACAGCC CAAGGATACT GCAACAAGTC CCTCCTCTGT CACTGCAGAA TAACCAGCTA   781 CGTAGAAATC TGATGGAAAT ACCCTGAGTT CTTGCAAGTC TTTGCAACTG GAGGCCACCA   841 CTTGCAAGCC TTTGTCCGAG ATACAATCCA GTACCCAAAG GCGTTGGAGC TTCACACAGC   901 GGCTAATCAT TTTTTGTAAG GTCAGAAGGC ATCAAAGTGT GGGGTGCATA GCCTCAAGTT   961 CAATCCTGTC AGTTGAGCAA CACAAGGGGG GTAGAATAAA TGACAGGCAA ACCAGGAGAA  1021 GCATCCAGAA TCAGACAACT CCTCAACCAT CCTGGCATTT CTCAGAGACA CTTGGTAGCC 1081 TTTAAAGTTA GGACTCAGTT GGAAGTCAAT CCTGG //
Primer: SV40pro_F Sequence file: RDB16762_A8K6c.seq
>D06043A2_A8K6_2_SV40pro_F_G06_21_ABI24.ab1     1 CCTGGTCCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT   121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAAGC   961 GACGCCCAAC CCTGCCCATC ACGAGATTTC GATTTCCACC GCCGCCTTCT ATGAAGGTTG  1021 GGCTTTCGGA ATCGGTTTTC CGGGACGCCG CTGGAATGGA TTCTTCAAGC GCGAGCATCT 1081 CATGCTGGAA GCTCATCGCC CAACCCCAAC CTTTGGTCCA T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl