Sequencing and PCR primers for primer information.
Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR).
| Catalog number | RDB16762 |
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| Resource name | pcDNA-Ostir1-pA |
| Clone info. | Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR) mRNA synthesis. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence. Expression was confirmed by the depositor with EGFP monitoring by microinjectioning its mRNA with hPLCz-AID-EGFP mRNA into mouse oocytes and culturing them in the presence of indole-3-acetic acid. |
| Vector backbone | pcDNA3.1-pA83 (Plasmid) |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells). |
| Gene/insert name | Oryza sativa OsTIR1 cDNA |
| Depositor | Ogura, Atsuo | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) is requested. |
|---|---|
| Additional terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16762 | pcDNA-Ostir1-pA | DNA solution |
Electronic file
Featured content
References
| original | Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16762_A8K6p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB16762_A8K6a.seq |
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>D06043A2_A8K6_2_CMV-Forward_E06_15_ABI24.ab1 1 AGGTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTCG AATTATGACG TACTTCCCGG 181 AGGAGGTGGT GGAGCACATC TTCAGCTTCC TGCCGGCGCA GCGCGACCGC AACACGGTCT 241 CGCTCGTCTG CAAGGTGTGG TACGAGATCG AGAGGCTGAG CCGCCGCGGC GTCTTCGTGG 301 GCAACTGCTA CGCCGTGCGC GCCGGCCGCG TCGCCGCGCG GTTCCCCAAC GTGCGGGCGC 361 TCACGGTGAA GGGGAAGCCC CACTTCGCCG ACTTCAACCT CGTGCCCCCC GACTGGGGCG 421 GCTACGCGGG GCCGTGGATC GAGGCGGCCG CGAGGGGATG CCACGGCCTG GAGGAGCTCA 481 GGATGAAGCG GATGGTGGTG TCCGACGAGA GCCTCGAGCT GCTGGCTCGC TCGTTCCCGC 541 GGTTCAGGGC TCTTGTTCTT ATCAGCTGCG AGGGGTTCAG CACTGACGGG CTAGCCGCCG 601 TCGCGAGCCA TTGCAAGCTT CTGAGGGAGT TGGATTTGCA GGAAAATGAA GTGGAGGATC 661 GAGGGCCTAG TGGCTTTCCT GCTTCCCTGA TTCCTGCACA TCACTGTCTC ATGATTTTGC 721 CTGCATCAAA GGGGAGGTAT GCTGTCCACT GGAGAGACTT GTTAGCAGGT CCCCAAACTG 781 GCGGAGTTTG AGGCTGATCG ATCTTGTATC GTAGAATACA CTTGCAAGAT ACTACTGCGT 841 ACCCCTACTG GAGGATTGGG GAATGGGTAT TTGGACGGAT GACTTCAACC TGAATCCTCA 901 TTTAAGCTAC CAGGCCTGGA ATGCCAATGT GAGATGCTGG AATCCGGACC CTCCGATGCC 961 GTCATATATA C // |
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| Primer: GNP_primer#10 | RDB16762_A8K6b.seq |
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>D06043A2_A8K6_2_GNP_Primer#10_F06_18_ABI24.ab1 1 TAATTTGTGC AAATTCAATT TAAAAGATAA CGTTTACAGG TTCTTTGGTG CCCATAGTCG 61 AGCGGCCTTA ACCACTAGCA GCAGAACCGG AGTCGACTAG GATTTTAACA AAATTTGGTG 121 CATCATCCCT CGCCCCAGCA GTTGTGCGGT ACACATATAA TTTCTCCACT TTAGGCAGAT 181 CTCCATGGTT TTCCTCCATT TCATTGCTAC CATCCCGCTC ATTTATGACC TCAACATTGA 241 GCATCGGCAT CTTTGACGCA AGGACTTGGC ACCCCTTTAA CGTGACATTG CAAGATGACA 301 TCCAAAGGGA TCGCATTGTC TCGTACCTAG CAAAATTCCC CAAGAGTGCA GCATCACCAA 361 ACGGGCTATC TCTTATCTCC AGTTTCCTTA AATTCTTGCA TCCATTCATA ACATGCATCA 421 TACCCTTATC ACTGTCACCA GCAAATGCTA TAGAAAGCAT CTCAAGTTGT TTTGCATATT 481 TCCCAATATA CATGAAAACT TTGTCTGTGA GAAGACCAGA TATTGACAAA CGTTGTAATC 541 CCTTGCACTC ACGAACAATA GCTCCAAAGC CTTCATCTAA TGGTTGGCTT GTCACAACAT 601 CAGGCTTCCC TGGCTCAAGA ATACAAAGTC TGAATCGTGT GAAATTTGGA CAGTTCTTGG 661 CGACAGTAAC TAGTGCAGCA TTAGTCATTT GGTGACAGAA GTACAGTAGT GAGTTCAGTT 721 TTGGACAGCC CAAGGATACT GCAACAAGTC CCTCCTCTGT CACTGCAGAA TAACCAGCTA 781 CGTAGAAATC TGATGGAAAT ACCCTGAGTT CTTGCAAGTC TTTGCAACTG GAGGCCACCA 841 CTTGCAAGCC TTTGTCCGAG ATACAATCCA GTACCCAAAG GCGTTGGAGC TTCACACAGC 901 GGCTAATCAT TTTTTGTAAG GTCAGAAGGC ATCAAAGTGT GGGGTGCATA GCCTCAAGTT 961 CAATCCTGTC AGTTGAGCAA CACAAGGGGG GTAGAATAAA TGACAGGCAA ACCAGGAGAA 1021 GCATCCAGAA TCAGACAACT CCTCAACCAT CCTGGCATTT CTCAGAGACA CTTGGTAGCC 1081 TTTAAAGTTA GGACTCAGTT GGAAGTCAAT CCTGG // |
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| Primer: SV40pro_F | RDB16762_A8K6c.seq |
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>D06043A2_A8K6_2_SV40pro_F_G06_21_ABI24.ab1 1 CCTGGTCCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAAGC 961 GACGCCCAAC CCTGCCCATC ACGAGATTTC GATTTCCACC GCCGCCTTCT ATGAAGGTTG 1021 GGCTTTCGGA ATCGGTTTTC CGGGACGCCG CTGGAATGGA TTCTTCAAGC GCGAGCATCT 1081 CATGCTGGAA GCTCATCGCC CAACCCCAAC CTTTGGTCCA T // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. |
|---|
2018.12.14
GNP_filter3_RDBDEP_html_181012.pl