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Oat CYP51H10/pBKS- (#RDB16759)

Cloning vector of oat cytochrome P450 family 51 subfamily H member 10 (CYP51H10), tagged with His at C-terminus.

Clone info. Cloning vector of oat cytochrome P450 family 51 subfamily H member 10 (CYP51H10), tagged with His at C-terminus.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Avena sativa CYP51G1 cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kunii, M et al. Biol. Pharm. Bull. 35(5), 801-04, 2012) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Kunii, M et al. Biol. Pharm. Bull. 35(5), 801-04, 2012）の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16759 Oat CYP51H10/pBKS- DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Oat CYP51H10/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16759).

Reference section:

Kunii, M., Kitahama, Y., Fukushima, E.O., Seki, H., Muranaka, T., Yoshida, Y., Aoyama, Y., beta-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. Biol. Pharm. Bull. 35 (5): 801-804 (2012). PMID 22687421. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16759_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16759_A8K1a.seq check
>D05837A7_A8K1_2_M13_-40_B03_06_ABI24.ab1
    1 AAGCCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG
   61 GCGGCCGCTC TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCGATCCG TCGACATGGA
  121 CATGACAATT TGCGTCGTTT GGTTGGTCTT AGCAATTATA TCCATCGCTG CAGTAGTATC
  181 CAAGAGTTCA AAGCGAAGCA ATGCCTCTGA TTCAGTGGTG ACACGACCAC CTCCACCGGT
  241 GGTGACAGGA ATTGATCTCC TCAAGTTCTT ACATGCTCTT TGTAGAAAGG ACCCTGAAGC
  301 TGCAATGATG TATCTGTATA ACAAGTTAGG CAGTATTTTC ACATTAAGTT TTTTGTGGAA
  361 AAGAGTAACC ATCTTGATTG GGCACGAGGC CTCCATTCCT TTCTTTCATG GTTTGGAGTC
  421 AGATGTTTCA CAAGGAAATT TCAATGAGTT CACCGTGCCA ATGTTCGGCA AAGAGAATGG
  481 GTATGCTGTG GAATATGCTA CTCGAATTGA GCAGTCTCGC TTCTTCTATG ATTCTCTAAA
  541 GGCATCGCAG CTGAGGAGCC ATGTTGATCT CATTCGACAG GAAGTGGAGG AGTACTTTGC
  601 AAAATGGGGA GACGAGGGTG AAGTCGATCT GAAACAAGAG TTCACCAAGT TACTCATGTT
  661 GATTGCTGGT CGCTGCCTAC TTGGAAGTGA GGTCCGAGAT ACGATATTTG GTGAGTTCTA
  721 CACATTGTTC GCTGATATTG AGGAGGGGGT CAACTTGTTC AGTTACATGT TCCCATATAT
  781 GCCGGTTCCA GTAAACAACC GACGAGACAG AGCACAAATG AAGCTTACAA GTATAGTGTC
  841 TGAGATTGTG AGGTCAAGAA AGAGATGCAA CCGCGTCGAG GATGATATGC TGCAGAGACT
  901 GATAGATTCC AGATATAAAG ATGGTCGTCC AACAACTGAA GGGGAGGTTT CCGGGATGAT
  961 CATTGGACTT ATATTTGCTG GAAAGCACAC AAGTACAATC ACTGCCTCCT GGACCGGAGC
 1021 TTGCCTTTTG ACCCATCCAA AATTCCTAGT GCTGCTGTCG AGGAGCAAAG CAATGATGAG
 1081 TAAATACAAG GATATATAGA CTACATATCC TGTCAGAAAA TGGAAGATTT GGCATAGGTG
1141 CCATCCAAGA AGGCAGTCGG ATGTAATCCC GCCAGCGGGC CCGGGGTGTT TGGC
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16759_A8K1b.seq check
>D05837A7_A8K1_2_reverse2_C03_09_ABI24.ab1
    1 AAGGAAAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATCCGCTAGC CTAATGGTGG
  121 TGATGATGGT GGTTTGCAGG CATACGACAT CTCTTGTAAC TTACCATTAC TTTGCCTTTA
  181 GGCTCTATTA TAAACTTGCT CCAATCTTGC TTGGGAAATG GAGAAGTCAG TTTAAGATCA
  241 AAATTTCTCA GCAAATGGCT CCAGATAATC TTTATTTGCA TGAAAGCAAA GGACTCGCCG
  301 GCGCAAGCAT GCCTTCCAGC ACCAAAAATT GTGTAAGAGA GTTTACCCCC AATTTTATCC
  361 TCCTCTCTTC CAACACGAAA CCGATCCGGA TCATAGACCT CGGGGTCCTT GTAAGTGTGT
  421 GGCACCTTAC CTGTTAGCAT TACAAGATGT GCTAACGTGG TACCTTTAGG GATACCATAT
  481 TCACCTCCCT CTCTTGTCTG CACACTGATC TCCTTCAGTG TCTTGCGCAG CAACACCGGC
  541 GCTGCGGGAT ACATCCGACC TGCCTCTTTG ATGCAACTAT GCAAAATCTC CATTTCTGAC
  601 AGGATATTGT AGTCTATATT ATCCTTGTAT TTACTCATCA TTTGCTTTTG CTCCTCGACA
  661 GCAGCACCTA GGAATTTTGG ATGGGTCAAA AGGCAAGCTC CGGTCCAGGA GGCAGTGATT
  721 GTACTTGTGT GCTTTCCAGC AAATATAAGT CCAATGATCA TCCCGGAAAC CTCCCCTTCA
  781 GTTGTTGGAC GACCATCTTT ATATCTGGAA TCTATCAGTC TCTGCAGCAT ATCATCCTCG
  841 ACGCGGTTGC ATCTCTTTCT TGACCTCACA ATCTCAGACA CTATACTTGT AAGCTTCATT
  901 TGTGCTCTGT CTCGTCGGTT GTTTACTGGA ACCGGCATAT ATGGGAACAT GTAACTGAAC
  961 AAGTTGACCC CCTCCTCAAT ATCAGCGAAC AATGTGTAGA ACTCACCAAA TATCGTATCT
 1021 CGGACCTCAC TTTCCAAGTA GGCAGCGACC AGCAATCAAC ATGAGTAACT TGGTGAACTC
 1081 TGTTCAGATC GACTTCACCT CGTCTCCCAT TTGCAAGGTA CTCTCACACT GTCGGAATGG
1141 AATCACATGC TTCTCATCTG CACTGCTATG GATCATAGGA GGAAGCGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kunii, M., beta-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. Biol. Pharm. Bull. 35 (5): 801-804 (2012). PMID 22687421. [link to RRC of NBRP]