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Rat CYP51A1m/pBKS- (#RDB16755)

Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1), modification of N-terminal region.

Clone info. Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGGTACTGC TGGGCTTGCT GCAG of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Rat CYP51A1 cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nitahara, Y et al. J. Biochem. 126(5), 927-33, 1999) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Nitahara, Y et al. J. Biochem. 126(5), 927-33, 1999）の引用を必要とする。

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RDB16755 Rat CYP51A1m/pBKS- DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Rat CYP51A1m/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16755).

Reference section:

Nitahara, Y., Aoyama, Y., Horiuchi, T., Noshiro, M., Yoshida, Y., Purification and characterization of rat sterol 14-demethylase P450 (CYP51) expressed in Escherichia coli. J. Biochem. 126 (5): 927-933 (1999). PMID 10544287. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16755_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16755_A8K1a.seq check
>D05837A3-A8K1-2_M13_-40_B01_02_ABI08.ab1
    1 GCAAGGGAAG CCGCGCCGTA ATACGGACTT CACTATAGGG GCGAATTGGA GCTCCACCGC
   61 GGTGGCGGCC GCTCTAGAAC TAGTGGATCC ATCGATGCTT AGGAGGTCAT ATGGCTCTGT
  121 TATTAGCAGT TTTTTCAGGC GGCTCGGTGC TCGGGCAGGC GATGGAGCAG GTGACAGGAG
  181 GCAACCTGCT TTCCACGCTG CTCATCGCCT GCGCCTTCAC GCTTAGCCTT GTCTACCTGT
  241 TCCGCCTCGC AGTGGGCCAC ATGGTCCAGC TGCCCGCTGG AGCGAAAAGT CCGCCATATA
  301 TTTACTCTCC AATTCCGTTC CTTGGCCACG CGATAGCATT TGGGAAAAGT CCAATTGAGT
  361 TCCTGGAAAA TGCATATGAG AAGTACGGAC CTGTGTTTAG CTTTACCATG GTGGGCAAGA
  421 CTTTCACTTA CCTTTTGGGG AGTGACGCAG CCGCCCTGCT CTTCAACAGT AAGAACGAAG
  481 ACCTGAATGC GGAAGAGGTC TACGGTCGGC TGACAACGCC CGTGTTTGGG AAGGGAGTTG
  541 CATACGACGT GCCAAATGCA GTTTTCCTGG AACAGAAGAA AATACTAAAA AGCGGCCTTA
  601 ACATAGCCCA CTTCAAGCAG TATGTTTCTA TCATTGAAAA AGAAGCGAAG GAGTACTTTA
  661 AAAGTTGGGG AGAAAGCGGA GAAAGAAATG TGTTTGAAGC GCTGTCTGAG CTCATAATTT
  721 TAACAGCTAG CCATTGCTTA CATGGAAAGG AAATTAGAAG TCAGCTCAAC GAGAAGGTGG
  781 CTCAGCTGTA CGCAGACCTG GATGGAGGCT TTAGCCATGC CGCCTGGCTG TTGCCAGGCT
  841 GGCTGCCCCT GCCGAGTTTC AGGCGCAGGG AACAGAGCCC ATCGAGAGAT CAAGGAATAT
  901 TTTCTACAAG GCTCATCCAG AAACGTAGGC TGTCAAAAGA ACCCAAGCCG GAGGAACAAT
  961 TCTTCAAACT TTACTGGATT CTACATACAA GGATGGGCGT TCTCTGAACG GACGGACGAA
 1021 GATCGCAGGG ATGCTCATCG GACTGCTGGC TGGGCGGAAC AGCACACAAT TCCTCCACAA
1081 CCAGTGACCT GGATGGGGCG TTCCTTCCAT GGGCA
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16755_A8K1b.seq check
>D05837A3_A8K1_2_reverse2_C02_08_ABI24.ab1
    1 AATGAAAATG AAATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TCATTCTCTG CCAAGCTGCA
  121 GCTTGCAGTC CCGTCTCCAC ACTGGCTCCT TGTTCCTTTC TTCATTTTGA TCTTCGTTTG
  181 TAACGGATTA CTGGGTTTTC TGGGGTATGA ATCATTGTTG TATAATTCAC ACTGGGAAAA
  241 TATCCATTGA TGAGGTCAAA TTCATATAAA CGAAGCATAG TGGACCAAAT TGTCTTAATT
  301 TGAACATAGG CAAAATTTTC TCCAATACAA CGATGGCGCC CGGCTCCAAA CGGCACATAG
  361 GCAAACTTCT CTCCCGACGC TGGGTTGTCC TGTAGGTAGC GGTCAGGATT GAAGTCCAAG
  421 CGTTCTACCC AGGAGTCTTT CAGTCTTTGA TTGACAGTTG GAGAAACACA CACCTGATGT
  481 CCTGGAGGAA TGGTGTACCC AGCCACAGTC TGAGGGGTCT TGGCCATTCT CATCATGGTC
  541 ATTATAGGAG GCCTCAGTCT TAGTGTTTCT TTTATGCATC TATCAAGTAA ATTCAGATCC
  601 TTCAACTGCT CATAAGTTAA GGGAGGCAGA TCCTCCCCGC ACACCGTTTT CTGTTCTAAG
  661 TAGCATTTGT CTTGAAGTGG TTTATCTCTG GCCAGAAAGA AGCCCATCCA GGCACTGGTG
  721 GTGGAGGATG TGTGCTGTCC GGCCAGCAGC AGTCCGATGA GCATCCCTGC GATCTCGTCG
  781 TCCGTCAGAG GACGCCCATC CTTGTATGTA GAATCCAGTA AAGTTTGAAG AATGTCCTCG
  841 GCTGGTTCTT TTGACAGCCT ACGTTTCTGG ATGGCCTTGT AGAAAATATT CTTGATCTCT
  901 CGATGGGCTC TGTCCCTGCG CCTGAAACTC GGCAGGGGCA GCCAGCCTGG CAACAGCCAG
  961 GCGGCATGGC TAAAGCCTCC ATCCAGGTCT GCGTACAGCT GAGCCACCTT CTCGTTGAGC
 1021 TGACTTCTAA TTTCCTTTCC ATGTAGCAAT GGCTAGCTGT AAATATGAGC TCAGACAGCG
 1081 CTTCAAACAC ATTCTTTCTC GCTTTTCTCC CCAACTTTTA GTACTTCGTC GCTCCTTTTC
1141 AATGATAGAA AACAATACTG CGTTGAAAGT TGGGCCTTAT GGATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nitahara, Y., Purification and characterization of rat sterol 14-demethylase P450 (CYP51) expressed in Escherichia coli. J. Biochem. 126 (5): 927-933 (1999). PMID 10544287. [link to RRC of NBRP]