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Rice CYP51G1m/pBKS- (#RDB16753)

Cloning vector of rice cytochrome P450 family 51 subfamily G member 1 (CYP51G1), modification of N-terminal region.

Clone info. Cloning vector of rice cytochrome P450 family 51 subfamily G member 1 (CYP51G1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGGATCTCG CCGACCCAAA CCAC of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Rice LOC4350614 cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16753 Rice CYP51G1m/pBKS- DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Rice CYP51G1m/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16753).

Reference section:

, Master thesis.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16753_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16753_A8K1a.seq check
>D05837A1_A8K1_3_M13_-40_F01_16_ABI24.ab1
    1 AGGCAGCGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG
   61 CGGCCGCTCT AGAACTAGTG GATCCATCGA TGCTTAGGAG GTCATATGGC TCTCTTATTA
  121 GCAGTTTTTC GGCTCATCGC CGGCGCGGCG CTGCTGGTGG CCACCCTCGC CTTCATCAAG
  181 CTGCTGCTGA GCTCCGCCGG CGGCGGCAAG AAGCGCCTCC CGCCGACCAT CCCGGCGGCG
  241 CCGCTGGTGG GCGGGCTGCT CCGCTTCATG CGGGGGCCGA TCCCGATGAT CCGGGAGGAG
  301 TACGCCCGCC TCGGCAGCGT CTTCACCGTC CCCATCCTCA GCCGCAAGAT CACCTTCCTC
  361 ATCGGCCCCG AGGTGTCGGC GCACTTCTTC AAGGGGAACG AGGCCGAGAT GAGCCAGCAG
  421 GAGGTCTACA AGTTCAACGT GCCCACCTTC GGCCCTGGCG TCGTCTTCGA CGTCGACTAC
  481 TCCGTCAGGC AGGAGCAGTT CAGGTTCTTC ACCGAGGCGC TCCGCGCCAA CAAGCTCCGC
  541 AGCTATGTCG ACCAGATGGT TGTCGAGGCT GAGGAGTACT TCTCTAAGTG GGGAGAAAGT
  601 GGAACGGTGG ATCTGAAGTA TGAGCTGGAG CATCTCATCA TTCTGACTGC TAGTCGGTGC
  661 TTGCTCGGGA GGGAGGTGCG AGAAAAGCTC TTTGATGATG TTTCTTCTCT CTTCCATGAT
  721 CTGGACAATG GGATGCAGCC AGTCAGTGTC ATCTTCCCCT ACCTCCCAAT TCCTGCACAC
  781 CGCCGCCGTG ACCGGGCACG GCAACGGTTG AAGGAAATCT TCGCCACCAT CATCAAGTCC
  841 CGCAAGGCCT CTGGACGGGC TGAGGAAGAC ATGCTGCAGT GCTTCATTGA TTCGAAGTAC
  901 AAGAGTGGAC GCTCAACCAC AGAAAGTGAA ATAACCGGCC TACTTATTGC AGCACTATTT
  961 GCTGGACAGC ACACTAGCTC TATCACCTCA ACCCTGGACT GGGGCCTACA TGCTTCGCTT
 1021 TCAAGCAGTA CTTCGCAGCA GCCGAAGAGA GCAGAGAGGT CATGAAGCGG CACGGGGACA
 1081 GATCGGATCA TGACATCTTG GCAGAGATTG ATGGTCTCTA CAGTGCATCA GGAGCGCCTC
1141 GGTCTTACCT ACGCTAATCA TGCCTGACTT GCCGGTCCGA
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16753_A8K1b.seq check
>D05837A1_A8K1_3_reverse2_G01_19_ABI24.ab1
    1 AAGGACATGA TTACGCCAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGGTAC
   61 CGGGCCCCCC CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGCCCGG
  121 GGGATCCTAA AACGACTGAA ACACCCTAAT ACAAAGCTGG CAGAAACTTA CACGGATCAA
  181 ATACTGACAG AAAGGGAAAA GAAGAGAAAA AAAGGCGAAA AGCGCGACAA GCATCGACTC
  241 CTAAGGTTGA AGGTGGCATC CTAGTTGTCG ACGACGAGCT TCCGCCGCTT GAAGTTGACC
  301 ATCACCTCGT CCTTGATGCC GACGACCATG GCTTTCCAGT TGGTCTCCGG GAAGGGGGAG
  361 ACCAGCTCGA ACTCAAAGTT CCTCAGCAGG TGAGTCCATA TGGCCTTGAT CTGTAGGTAG
  421 GCGAAGGGCT CACCGAGGCA ACCGTGCCTG CCGCCACCAA AGGAGATGTA CGAGAAGGCG
  481 CCCGCTGCCT TGTCCTCCTC CCTGCCTGGT GCGTAGCGGT CGGGGTCGTA TGAGTCGGGG
  541 TTCTTGAAGA TGTGAGGGAG CCTGTTGGCG AAGGCTGGAG ATGTCGCGAC GATGTGGCCC
  601 TTCGGGATGT CGAACTCTTT GCCATCCTTC GTTGTCACTG AGAAGTCGTT GTGTGACTGG
  661 CGGAGCAGCA TGATTAGCGG TGGGTGAAGA CGGAGCGCCT CCTTGATGCA CCTGTAGAGG
  721 ACATCCATCT CTGCCAAGAT GTCATGATCG ATCTTGTCCC CGTGCCGCTT CATGACCTCC
  781 TTCTGCTCCT CTTCGGCTGC TGCGAAGTAC TGCTTGAAGC GAAGCATGTA GGCCCCAGTC
  841 CAGGTTGAGG TGATAGAGCT AGTGTGCTGT CCAGCAAATA GTGCTGCAAT AAGTAGGCCG
  901 GTTATTTCAC CTTCTGTGGT TGAGCGTCCA CTCTTGTACT TCGAATCAAT GAAGCACTGC
  961 AGCATGTCTT CCTCAGCCCG TCCAGAGGCC TTGCGGGACT TGATGATGGT GGCGAAGATT
 1021 TCCCTTCAAC CGTTTGCCCG TGCCCGGTCA CGGCGGCGGT GTGCAGGAAT TGGGAGGTAG
 1081 GGGAAGATGA CACTGACTGG CCTGCATCCC ATTGTCCAGA TCATGCAGAG GAGACCATCA
1141 TCAAGAGCTT TTTCTTCGCC AACGTTCCTT CCCGGGAGTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original , Master thesis.