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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-ChRGR(ER)-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16750
Resource name pCAGGS-ChRGR(ER)-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver with an ER-retention motif (ChRGR(ER)), tagged with Venus at C-terminus, CAG promoter. Codon optimized for human. To generate a ChRGR(ER), a cDNA encoding Gln4765-Ile4866 of mouse ryanodine receptor 2 (NM_023868) was inserted in-frame between ChRGR and Venus using the AgeI restriction site.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of insert sequence LC415030 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |
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External Database
Chlamydomonas reinhardtii acop1 (channelopsin-1) Chlamydomonas reinhardtii cop4 (acop2)

        Reference sequence
          NM_023868

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Asano, T., Front. Neurosci. 12: 561, 2018)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
        付加的提供条件 Venus の使用について、宮脇敦史博士の書面による提供承諾を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB16750 pCAGGS-ChRGR(ER)-Venus DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0087e (English text)
        Featured content Dnaconda's recommendation EXR0087j (Japanese text)
        Featured content Genetic Resources for Optgenetics Research (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16750_A8Jnp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pAxCA_F1
        Region: kozak seq,insert 5'(ChRGR(ER) 5')
        Sequence file: RDB16750_A8Jnf.seq check
        >D05919A2_A8Jn_2_pAxCA-F1_G03_21_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNT GCCTTCTTCT TTTTCCTACN GCTCCTGGGC AACGTGCTGG
           61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGCCAC CATGGCTCGG AGACCTTGGC
          121 TGCTGGCACT GGCTCTGGCT GTCGCTCTGG CCGCTGGCTC TGCTGGGGCT TCTACTGGAT
          181 CTGACGCAAC AGTGCCCGTC GCTACTCAGG ACGGACCTGA TTACGTGTTC CACAGGGCAC
          241 ATGAGCGCAT GCTGTTTCAG ACATCTTATA CTCTGGAAAA CAATGGCAGT GTCATCTGCA
          301 TTCCCAACAA TGGGCAGTGC TTCTGTCTGG CATGGCTGAA GTCTAACGGC ACCAATGCCG
          361 AGAAACTGGC CGCTAACATC CTGCAGTGGA TTACATTCGC CCTGTCCGCT CTGTGCCTGA
          421 TGTTTTACGG ATATCAGACC TGGAAGTCTA CATGTGGCTG GGAGGAAATC TACGTGGCTA
          481 CCATCGAGAT GATCAAGTTC ATCATTGAAT ACTTCCACGA GTTTGACGAA CCTGCCGTGA
          541 TCTATAGCTC CAACGGGAAT AAGACTGTCT GGCTGAGATA CGCTGAGTGG CTGCTGACCT
          601 GTCCAGTGAT CCTGATTCAT CTGAGCAACC TGACTGGCCT GGCCAACGAC TATAATAAGA
          661 GAACCATGGG GCTGCTGGTG AGTGATATCG GAACAATTGT CTGGGGCACC ACAGCAGCCC
          721 TGTCAAAAGG CTATGTGCGC GTCATCTTCT TTCTGATGGG CCTGTGCTAC GGGATCTACA
          781 CATTCTTTAA TGCTGCAAAG GTGTACATCG NGGCATATCA CACAGTCCNT AAAGGGCGGT
          841 GTAGACAGGT GGTCACTGGA ATGGCCTGNC TGTNCTTTGT GTNCTGGGGG ANGTTNCNNA
          901 TCCNGNTTCT GCTGGGNNCN GAANNNNNCN GCCNNNNNNA NNNGTTTNNN NCAGCCNTCG
        961 CNNATNCNNN TCTGNNNNNN NNNN
        //
        Primer: Venus-N_R
        Region: insert mid(Venus 5',ChRGR(ER) 3')
        Sequence file: RDB16750_A8Jng.seq check
        >D05919A2_A8Jn_2_venus-N_R_H03_24_ABI24.ab1
            1 NNNNNNNNNN NNCNNNNTTG TGGNCGTTNN NGTCGCCGTC CNGCTCGACC AGGNTGGGCA
           61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT ATGATCAAAC
          121 CTTGTATGAT AGCCAAAAGG ATGACAATCA CGAAGAAGAA GAATGTGATG TCAAAGATGA
          181 TGCGGTAGAT CTCATACTCG TCTCCTGCTG GGTCTTCAAT TTCATCACCA ATGCCCCCTC
          241 CAGCACGGAC GCCCACATAC ATGTGGAACA TGTAACACGT CAGCATGTCA TCACACTTCA
          301 TGTCTGGCGT GTCACCATCC TCACTTTTAT TGTAGAATTT TCGGAAGAAA TTGAACGCCA
          361 CGACAGTGTA CAGGTACACA ACAACAGCCA ATAAGCCAAC AGTTAACACG AGCTGTACCG
          421 GTGGATCCTT CCCAGTCCCT TTGTTCACGG CTCCTGCCTC GGCTTCATCT TCCACCAGTG
          481 TTTCCACCTC AATTTCGGTC CCGCCGATAT TCAGTTTGGT AGTCTTCCGA ATATCGCCAT
          541 GAATCAGGAT ATGCTCGTGG ATCAGCACTC GCAGGTAGTG TCCCAGCAGC CCCCAGCAGT
          601 TTTTGCTGGC CAGGTCCAGA ATTGCATGAG CGATGGCTGA ATTAAACTGG TTAATGTGGC
          661 CGAATCCTTC GGGTCCCAGC AGAAACAGGA TTGGGAACAT CCCCCAGGAC ACAAAGAACA
          721 GCCAGGCCAT TCCAGTGACC ACCTGTCTAC ACCGCCCTTT ANGGACTGTG TGATATGCCT
          781 CGATGTACAC CTTTGCAGCA TTNAAGAATG TGTAGATCCC GTANCACANG CCCATCAGAA
          841 AGAANANGAC GCGCACATAG CCTTTTGACA GGGCTGCTGN GGTGCCCCAG ANAATTGTNN
          901 CGANATCACT CACCANCAGC CCCANNNTTC TNTNATTATA NNCGTNNNNC ANGCNANNNN
        961 NNTNGNNNAN NNGNNNNNNN ATCANNNGNN NNNGNNNNCN NNNNNNNNN
        //
        Primer: reverse
        Region: beta globin pA,insert 3'(Venus 3')
        Sequence file: RDB16750_A8Jnh.seq check
        >D05919A2_A8Jn_2_reverse_H02_23_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNT AAGAGAAGAG GGNNAGCTAT GACTGGGAGT
           61 AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA AGGAAAATTT TAGGGATGTT
          121 AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT AACCTCAAGT CAAGGCTTTT
          181 CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT ACTGATGACC TCTTTATAGC
          241 CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC
          301 TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA
          361 AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC
          421 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT
          481 TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
          541 GGAGTGAATT CTCATTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
          601 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC
          661 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
          721 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAN
          781 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
          841 TTGTACTCCA GCTTGTGCCN CANGANGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCANC
          901 TCNATGCGGN TCACCNNGGT GTCGCCCNCG AACTTCNCCT CGGCGCGGGN CTNGTANTTG
          961 NCGNCNNCCN NGNNGAANNN GGNGNNNNNC NNNNNNGTAN CCNTCNGNNN TNNNNGNNNN
        1021 TNNNNNNNNN N
        //
        Primer: SV40pro_F_V2
        Region: SV40 pA,ori
        Sequence file: RDB16750_A8Jni.seq check
        >D05919A2_A8Jn_2_SV40pro_F_V2_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCTT TTGNNNNNGC TAACTTGTTT ATTGCAGCTT
           61 ATAATGGTTA CAANNTNNNN GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
          121 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
          181 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
          241 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
          301 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
          361 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
          421 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
          481 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
          541 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
          601 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
          661 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
          721 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
          781 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACNNNNT TCTTGAAGTG GTGGCCTAAC
          841 TACNGCTACA CTANANNAAC AGTATTTGGT ATCTGCGCTC TGCTGAANCC NGTTACNTTC
          901 GGAAAAAGAN TTGGTAGCTC TTGATNCNNC AAACAAACCA NNNCNGGTAN CGGNGGNTTT
        961 TTTTTGTTTG NNNGCAGCNG ATTANNCGCA NAAAAAAAAN GNNCNNNNNN NNNN
        //
        Primer: EGFP-M_R
        Region: insert mid(Venus 5',ChRGR(ER) 3')
        Sequence file: RDB16750_A8Jnj.seq check
        >16750_16750_B1JcF_EGFP-M_R_E03_15_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GGNNCTTGNN GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
           61 GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
          121 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
          181 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
          241 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
          301 GTAGCGGGCG AAGCACTGCA GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
          361 GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
          421 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
          481 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTAT
          541 GATCAAACCT TGTATGATAG CCAAAAGGAT GACAATCACG AAGAAGAAGA NNGTGATGTC
          601 NAAGATGANG CGGTAGATCT CATACTCGTC TCCTGCTGGG TCTTCANTTT CATCACCNAN
          661 GCCCCCTCCN GCACGGNNNC CCACATACAT GTGGNNNATG NNNNACNNNA GCATGNNNNN
        721 NNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl