Resource data sheet
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pCAGGS-ChRGR(ER)-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16750
Resource name pCAGGS-ChRGR(ER)-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver with an ER-retention motif (ChRGR(ER)), tagged with Venus at C-terminus, CAG promoter. Codon optimized for human. To generate a ChRGR(ER), a cDNA encoding Gln4765-Ile4866 of mouse ryanodine receptor 2 (NM_023868) was inserted in-frame between ChRGR and Venus using the AgeI restriction site.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of nucleotide sequence LC415030 (DDBJ accession number)
Depositor Ishizuka, Toru |
 
Other clones in our bank Chlamydomonas reinhardtii COP3 | Chlamydomonas reinhardtii COP4 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
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Please obtain written approval of Dr. Atsushi Miyawaki.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16750 pCAGGS-ChRGR(ER)-Venus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0087j (Japanese text)
Featured content Genetic Resources for Optgenetics Research (English text)
Featured content Dnaconda's recommendation EXR0087e (English text)

References

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16750_A8Jnp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB16750_A8Jna.seq
>D05919A3_A8Jn_1_pAxCA_F1_A02_01_ABI08.ab1
    1 GGACTCCTGT CCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCGC CACCATGGCT CGGAGACCTT
  121 GGCTGCTGGC ACTGGCTCTG GCTGTCGCTC TGGCCGCTGG CTCTGCTGGG GCTTCTACTG
  181 GATCTGACGC AACAGTGCCC GTCGCTACTC AGGACGGACC TGATTACGTG TTCCACAGGG
  241 CACATGAGCG CATGCTGTTT CAGACATCTT ATACTCTGGA AAACAATGGC AGTGTCATCT
  301 GCATTCCCAA CAATGGGCAG TGCTTCTGTC TGGCATGGCT GAAGTCTAAC GGCACCAATG
  361 CCGAGAAACT GGCCGCTAAC ATCCTGCAGT GGATTACATT CGCCCTGTCC GCTCTGTGCC
  421 TGATGTTTTA CGGATATCAG ACCTGGAAGT CTACATGTGG CTGGGAGGAA ATCTACGTGG
  481 CTACCATCGA GATGATCAAG TTCATCATTG AATACTTCCA CGAGTTTGAC GAACCTGCCG
  541 TGATCTATAG CTCCAACGGG AATAAGACTG TCTGGCTGAG ATACGCTGAG TGGCTGCTGA
  601 CCTGTCCAGT GATCCTGATT CATCTGAGCA ACCTGACTGG CCTGGCCAAC GACTATAATA
  661 AGAGAACCAT GGGGCTGCTG GTGAGTGATA TCGGAACAAT TGTCTGGGGC ACCACAGCAG
  721 CCCTGTCAAA AGGCTATGTG CGCGTCATCT TCTTTCTGAT GGGCCTGTGC TACGGGATCT
  781 ACACATTCTT TAATGCTGCA AAGGTGTACA TCGAGGCATA TCACACAGTC CCTAAAGGGC
  841 GGTGTAGACA GGTGGTCACT GGAATGGCCT GGCTGTTCTT TGTGTCCTGG GGGATGTTCC
  901 CAATCCTGTT TCTGCTGGGA CCCGAAAGAT TCGGCCACAT TAACCAGTTT AAATTCAGCC
  961 CATCGCTCAT GCAATTCTGG GACCCTGCCA GCAAAAAAAC TGCTGGGGGG CTGCTGGGGA
 1021 CACTACCCTG GCGAGTTGCT GAATTCAACG AGCCATATCC CTTGATTCCA TGGCGATATT
1081 CGGAAGAACC TACCGAAACT TGAAATAATT CCG
//
Primer: Venus-N_R RDB16750_A8Jnb.seq
>D05919A3_A8Jn_1_Venus-N_R_B02_02_ABI08.ab1
    1 GTCTCCCCGG ACGGCCTGTA CTTGTGGCCG TTTACGTCGC CGTTCCAGCT CGACCAGGAT
   61 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC
  121 CTTCCCAGTC CCTTTGTTCA CGGCTCCTGC CTCGGCTTCA TCTTCCACCA GTGTTTCCAC
  181 CTCAATTTCG GTCCCGCCGA TATTCAGTTT GGTAGTCTTC CGAATATCGC CATGAATCAG
  241 GATATGCTCG TGGATCAGCA CTCGCAGGTA GTGTCCCAGC AGCCCCCAGC AGTTTTTGCT
  301 GGCCAGGTCC AGAATTGCAT GAGCGATGGC TGAATTAAAC TGGTTAATGT GGCCGAATCC
  361 TTCGGGTCCC AGCAGAAACA GGATTGGGAA CATCCCCCAG GACACAAAGA ACAGCCAGGC
  421 CATTCCAGTG ACCACCTGTC TACACCGCCC TTTAGGGACT GTGTGATATG CCTCGATGTA
  481 CACCTTTGCA GCATTAAAGA ATGTGTAGAT CCCGTAGCAC AGGCCCATCA GAAAGAAGAT
  541 GACGCGCACA TAGCCTTTTG ACAGGGCTGC TGTGGTGCCC CAGACAATTG TTCCGATATC
  601 ACTCACCAGC AGCCCCATGG TTCTCTTATT ATAGTCGTTG GCCAGGCCAG TCAGGTTGCT
  661 CAGATGAATC AGGATCACTG GACAGGTCAG CAGCCACTCA GCGTATCTCA GCCAGACAGT
  721 CTTATTCCCG TTGGAGCTAT AGATCACGGC AGGTTCGTCA AACTCGTGGA AGTATTCAAT
  781 GATGAACTTG ATCATCTCGA TGGTAGCCAC GTAGATTTCC TCCCAGCCAC ATGTAGACTT
  841 CCAGGTCTGA TATCCGTAAA ACATCAGGCA CAGAGCGGAC AGGGCGAATG TAATCCACTG
  901 CAAGGATGTT AGCGGCCAGT TTCTCGGCAT TGGTGCCGTT TAGACTTCAG CCATGCCAGA
  961 CAGAAGCACT GCCCAATTGT TTGGGAAATG CAGATGACAC TGCCATTGTT TTTCCCAGAG
 1021 TATAAGGATG TTCTGAACAG CAATGGCGCT CATGTGCCTG TGACACGTAT CAAGATCCGT
1081 CCTGGAG
//
Primer: reverse RDB16750_A8Jnc.seq
>D05919A3_A8Jn_1_reverse_C02_03_ABI08.ab1
    1 AAGGAAGGGC CGGCCAGGTC GGAGGGGATC TCCATAAGAG AAGAGGGACA GCTATGACTG
   61 GGAGTAGTCA GGAGAGGAGG AAAAATCTGG CTAGTAAAAC ATGTAAGGAA AATTTTAGGG
  121 ATGTTAAAGA AAAAAATAAC ACAAAACAAA ATATAAAAAA AATCTAACCT CAAGTCAAGG
  181 CTTTTCTATG GAATAAGGAA TGGACAGCAG GGGGCTGTTT CATATACTGA TGACCTCTTT
  241 ATAGCCAACC TTTGTTCATG GCAGCCAGCA TATGGGCATA TGTTGCCAAA CTCTAAACCA
  301 AATACTCATT CTGATGTTTT AAATGATTTG CCCTCCCATA TGTCCTTCCG AGTGAGAGAC
  361 ACAAAAAATT CCAACACACT ATTGCAATGA AAATAAATTT CCTTTATTAG CCAGAAGTCA
  421 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCTCAGT
  481 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA
  541 CCTGAGGAGT GAATTCTCAT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
  601 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
  661 CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
  721 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT
  781 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT
  841 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAA GTCGATGCCC
  901 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCCTCGG CGCGGGTCTT
  961 GTAGTTTGCC GTCGTCCTTT GAAGAAGATG GTTGCGCTCC CTGGACGTAG CCTTTCGGGG
 1021 CATGGCGGAC TTTGGAGAGG TCGTGCTGGC TTTCAATGTG GTCAGAGTAG CGGGGCGAGG
1081 CACTTGCAAG TCGTTAGCCA AGTTGTTCAC GAAGGATGGG GCCAAG
//
Primer: SV40pro_F RDB16750_A8Jnd.seq
>D05919A3_A8Jn_1_SV40pro_F_D02_04_ABI08.ab1
    1 TCGCCGGGGT TTAGCCTTTC CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA
  121 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC
  181 AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG
  241 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
  301 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
  361 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
  421 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
  481 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
  541 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
  601 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
  661 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
  721 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
  781 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
  841 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTTGGTATC
  901 TGCGCTCTGC TGAAGCCAGT TACCTTTCGG AAAAAGAGTT TGGTAGCTCT TGATCCGGCA
  961 AACAAACCCA CCGCTGGTAG CGGTGGTTTT TTTTGTTTGC AAGCAGCAGA TTACGCCGCA
 1021 AGAAAAAAAG GGATCTCAAG GAGGATCCTT TGATCTTTCT ACGGGGTCTG AACGCTTCAG
1081 TTGGAAACCG AAAGCTTCTC G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

2019.03.25

GNP_filter3_RDBDEP_html_190320.pl