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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-ChRGR(ER)-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16750
Resource name pCAGGS-ChRGR(ER)-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver with an ER-retention motif (ChRGR(ER)), tagged with Venus at C-terminus, CAG promoter. Codon optimized for human. To generate a ChRGR(ER), a cDNA encoding Gln4765-Ile4866 of mouse ryanodine receptor 2 (NM_023868) was inserted in-frame between ChRGR and Venus using the AgeI restriction site.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of nucleotide sequence LC415030 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Regarding the use of Venus, a written approval of Dr. Atsusi Miyawaki is required.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
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RDB16750 pCAGGS-ChRGR(ER)-Venus DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0087e (English text)
Featured content Dnaconda's recommendation EXR0087j (Japanese text)
Featured content Genetic Resources for Optgenetics Research (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16750_A8Jnp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: kozak seq,insert 5'(ChRGR(ER) 5')
Sequence file: RDB16750_A8Jnf.seq check
>D05919A2_A8Jn_2_pAxCA-F1_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GCCTTCTTCT TTTTCCTACN GCTCCTGGGC AACGTGCTGG
   61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGCCAC CATGGCTCGG AGACCTTGGC
  121 TGCTGGCACT GGCTCTGGCT GTCGCTCTGG CCGCTGGCTC TGCTGGGGCT TCTACTGGAT
  181 CTGACGCAAC AGTGCCCGTC GCTACTCAGG ACGGACCTGA TTACGTGTTC CACAGGGCAC
  241 ATGAGCGCAT GCTGTTTCAG ACATCTTATA CTCTGGAAAA CAATGGCAGT GTCATCTGCA
  301 TTCCCAACAA TGGGCAGTGC TTCTGTCTGG CATGGCTGAA GTCTAACGGC ACCAATGCCG
  361 AGAAACTGGC CGCTAACATC CTGCAGTGGA TTACATTCGC CCTGTCCGCT CTGTGCCTGA
  421 TGTTTTACGG ATATCAGACC TGGAAGTCTA CATGTGGCTG GGAGGAAATC TACGTGGCTA
  481 CCATCGAGAT GATCAAGTTC ATCATTGAAT ACTTCCACGA GTTTGACGAA CCTGCCGTGA
  541 TCTATAGCTC CAACGGGAAT AAGACTGTCT GGCTGAGATA CGCTGAGTGG CTGCTGACCT
  601 GTCCAGTGAT CCTGATTCAT CTGAGCAACC TGACTGGCCT GGCCAACGAC TATAATAAGA
  661 GAACCATGGG GCTGCTGGTG AGTGATATCG GAACAATTGT CTGGGGCACC ACAGCAGCCC
  721 TGTCAAAAGG CTATGTGCGC GTCATCTTCT TTCTGATGGG CCTGTGCTAC GGGATCTACA
  781 CATTCTTTAA TGCTGCAAAG GTGTACATCG NGGCATATCA CACAGTCCNT AAAGGGCGGT
  841 GTAGACAGGT GGTCACTGGA ATGGCCTGNC TGTNCTTTGT GTNCTGGGGG ANGTTNCNNA
  901 TCCNGNTTCT GCTGGGNNCN GAANNNNNCN GCCNNNNNNA NNNGTTTNNN NCAGCCNTCG
961 CNNATNCNNN TCTGNNNNNN NNNN
//
Primer: Venus-N_R
Region: insert mid(Venus 5',ChRGR(ER) 3')
Sequence file: RDB16750_A8Jng.seq check
>D05919A2_A8Jn_2_venus-N_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNCNNNNTTG TGGNCGTTNN NGTCGCCGTC CNGCTCGACC AGGNTGGGCA
   61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT ATGATCAAAC
  121 CTTGTATGAT AGCCAAAAGG ATGACAATCA CGAAGAAGAA GAATGTGATG TCAAAGATGA
  181 TGCGGTAGAT CTCATACTCG TCTCCTGCTG GGTCTTCAAT TTCATCACCA ATGCCCCCTC
  241 CAGCACGGAC GCCCACATAC ATGTGGAACA TGTAACACGT CAGCATGTCA TCACACTTCA
  301 TGTCTGGCGT GTCACCATCC TCACTTTTAT TGTAGAATTT TCGGAAGAAA TTGAACGCCA
  361 CGACAGTGTA CAGGTACACA ACAACAGCCA ATAAGCCAAC AGTTAACACG AGCTGTACCG
  421 GTGGATCCTT CCCAGTCCCT TTGTTCACGG CTCCTGCCTC GGCTTCATCT TCCACCAGTG
  481 TTTCCACCTC AATTTCGGTC CCGCCGATAT TCAGTTTGGT AGTCTTCCGA ATATCGCCAT
  541 GAATCAGGAT ATGCTCGTGG ATCAGCACTC GCAGGTAGTG TCCCAGCAGC CCCCAGCAGT
  601 TTTTGCTGGC CAGGTCCAGA ATTGCATGAG CGATGGCTGA ATTAAACTGG TTAATGTGGC
  661 CGAATCCTTC GGGTCCCAGC AGAAACAGGA TTGGGAACAT CCCCCAGGAC ACAAAGAACA
  721 GCCAGGCCAT TCCAGTGACC ACCTGTCTAC ACCGCCCTTT ANGGACTGTG TGATATGCCT
  781 CGATGTACAC CTTTGCAGCA TTNAAGAATG TGTAGATCCC GTANCACANG CCCATCAGAA
  841 AGAANANGAC GCGCACATAG CCTTTTGACA GGGCTGCTGN GGTGCCCCAG ANAATTGTNN
  901 CGANATCACT CACCANCAGC CCCANNNTTC TNTNATTATA NNCGTNNNNC ANGCNANNNN
961 NNTNGNNNAN NNGNNNNNNN ATCANNNGNN NNNGNNNNCN NNNNNNNNN
//
Primer: reverse
Region: beta globin pA,insert 3'(Venus 3')
Sequence file: RDB16750_A8Jnh.seq check
>D05919A2_A8Jn_2_reverse_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNT AAGAGAAGAG GGNNAGCTAT GACTGGGAGT
   61 AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA AGGAAAATTT TAGGGATGTT
  121 AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT AACCTCAAGT CAAGGCTTTT
  181 CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT ACTGATGACC TCTTTATAGC
  241 CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC
  301 TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA
  361 AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC
  421 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT
  481 TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
  541 GGAGTGAATT CTCATTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
  601 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC
  661 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
  721 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAN
  781 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
  841 TTGTACTCCA GCTTGTGCCN CANGANGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCANC
  901 TCNATGCGGN TCACCNNGGT GTCGCCCNCG AACTTCNCCT CGGCGCGGGN CTNGTANTTG
  961 NCGNCNNCCN NGNNGAANNN GGNGNNNNNC NNNNNNGTAN CCNTCNGNNN TNNNNGNNNN
1021 TNNNNNNNNN N
//
Primer: SV40pro_F_V2
Region: SV40 pA,ori
Sequence file: RDB16750_A8Jni.seq check
>D05919A2_A8Jn_2_SV40pro_F_V2_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCTT TTGNNNNNGC TAACTTGTTT ATTGCAGCTT
   61 ATAATGGTTA CAANNTNNNN GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  121 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
  181 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
  241 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
  301 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
  361 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
  421 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
  481 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
  541 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
  601 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
  661 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
  721 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
  781 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACNNNNT TCTTGAAGTG GTGGCCTAAC
  841 TACNGCTACA CTANANNAAC AGTATTTGGT ATCTGCGCTC TGCTGAANCC NGTTACNTTC
  901 GGAAAAAGAN TTGGTAGCTC TTGATNCNNC AAACAAACCA NNNCNGGTAN CGGNGGNTTT
961 TTTTTGTTTG NNNGCAGCNG ATTANNCGCA NAAAAAAAAN GNNCNNNNNN NNNN
//
Primer: EGFP-M_R
Region: insert mid(Venus 5',ChRGR(ER) 3')
Sequence file: RDB16750_A8Jnj.seq check
>16750_16750_B1JcF_EGFP-M_R_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNCTTGNN GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
   61 GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  121 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  181 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  241 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  301 GTAGCGGGCG AAGCACTGCA GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  361 GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  421 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  481 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTAT
  541 GATCAAACCT TGTATGATAG CCAAAAGGAT GACAATCACG AAGAAGAAGA NNGTGATGTC
  601 NAAGATGANG CGGTAGATCT CATACTCGTC TCCTGCTGGG TCTTCANTTT CATCACCNAN
  661 GCCCCCTCCN GCACGGNNNC CCACATACAT GTGGNNNATG NNNNACNNNA GCATGNNNNN
721 NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

2021.10.19

GNP_filter3_RDBDEP_html_210618.pl