Resource data sheet
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pCAGGS-ChRGR(ER)-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16750
Resource name pCAGGS-ChRGR(ER)-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver with an ER-retention motif (ChRGR(ER)), tagged with Venus at C-terminus, CAG promoter. Codon optimized for human. To generate a ChRGR(ER), a cDNA encoding Gln4765-Ile4866 of mouse ryanodine receptor 2 (NM_023868) was inserted in-frame between ChRGR and Venus using the AgeI restriction site.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii cDNA
Reference of nucleotide sequence LC415030 (DDBJ accession number)
Depositor Ishizuka, Toru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Approval form [Word] by Dr. Atsushi Miyawaki
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16750 pCAGGS-ChRGR(ER)-Venus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0087j (Japanese text)
Featured content Genetic Resources for Optgenetics Research (English text)
Featured content Dnaconda's recommendation EXR0087e (English text)

References

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16750_A8Jnp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB16750_A8Jna.seq
>D05919A2_A8Jn_2_pAxCA-F1_G03_21_ABI24.ab1
    1 CTTCCTGGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
   61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGCCAC CATGGCTCGG AGACCTTGGC
  121 TGCTGGCACT GGCTCTGGCT GTCGCTCTGG CCGCTGGCTC TGCTGGGGCT TCTACTGGAT
  181 CTGACGCAAC AGTGCCCGTC GCTACTCAGG ACGGACCTGA TTACGTGTTC CACAGGGCAC
  241 ATGAGCGCAT GCTGTTTCAG ACATCTTATA CTCTGGAAAA CAATGGCAGT GTCATCTGCA
  301 TTCCCAACAA TGGGCAGTGC TTCTGTCTGG CATGGCTGAA GTCTAACGGC ACCAATGCCG
  361 AGAAACTGGC CGCTAACATC CTGCAGTGGA TTACATTCGC CCTGTCCGCT CTGTGCCTGA
  421 TGTTTTACGG ATATCAGACC TGGAAGTCTA CATGTGGCTG GGAGGAAATC TACGTGGCTA
  481 CCATCGAGAT GATCAAGTTC ATCATTGAAT ACTTCCACGA GTTTGACGAA CCTGCCGTGA
  541 TCTATAGCTC CAACGGGAAT AAGACTGTCT GGCTGAGATA CGCTGAGTGG CTGCTGACCT
  601 GTCCAGTGAT CCTGATTCAT CTGAGCAACC TGACTGGCCT GGCCAACGAC TATAATAAGA
  661 GAACCATGGG GCTGCTGGTG AGTGATATCG GAACAATTGT CTGGGGCACC ACAGCAGCCC
  721 TGTCAAAAGG CTATGTGCGC GTCATCTTCT TTCTGATGGG CCTGTGCTAC GGGATCTACA
  781 CATTCTTTAA TGCTGCAAAG GTGTACATCG AGGCATATCA CACAGTCCCT AAAGGGCGGT
  841 GTAGACAGGT GGTCACTGGA ATGGCCTGGC TGTTCTTTGT GTCCTGGGGG ATGTTCCCAA
  901 TCCTGTTTCT GCTGGGACCC GAAGGATTCG GCCACATTAA CCAGTTTAAT TCAGCCATCG
  961 CTCATGCAAT TCTGGACCTG GCCAGCAAAA ACTGCTGGGG GGCTGCTGGG ACACTACCCT
 1021 GCGAGTGCTG ATCCACGAGC ATATCCCTGA TTCATGGGCG ATATTTCGGA GACTACTAAA
1081 CTGAATATCG CGGACGGAAT GGAGGTGGAA CACTGATGGA AAGAATTGAA GGTCG
//
Primer: Venus-N_R RDB16750_A8Jnb.seq
>D05919A2_A8Jn_2_venus-N_R_H03_24_ABI24.ab1
    1 TGGTCGGGGA CGCTGACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA
   61 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT ATGATCAAAC
  121 CTTGTATGAT AGCCAAAAGG ATGACAATCA CGAAGAAGAA GAATGTGATG TCAAAGATGA
  181 TGCGGTAGAT CTCATACTCG TCTCCTGCTG GGTCTTCAAT TTCATCACCA ATGCCCCCTC
  241 CAGCACGGAC GCCCACATAC ATGTGGAACA TGTAACACGT CAGCATGTCA TCACACTTCA
  301 TGTCTGGCGT GTCACCATCC TCACTTTTAT TGTAGAATTT TCGGAAGAAA TTGAACGCCA
  361 CGACAGTGTA CAGGTACACA ACAACAGCCA ATAAGCCAAC AGTTAACACG AGCTGTACCG
  421 GTGGATCCTT CCCAGTCCCT TTGTTCACGG CTCCTGCCTC GGCTTCATCT TCCACCAGTG
  481 TTTCCACCTC AATTTCGGTC CCGCCGATAT TCAGTTTGGT AGTCTTCCGA ATATCGCCAT
  541 GAATCAGGAT ATGCTCGTGG ATCAGCACTC GCAGGTAGTG TCCCAGCAGC CCCCAGCAGT
  601 TTTTGCTGGC CAGGTCCAGA ATTGCATGAG CGATGGCTGA ATTAAACTGG TTAATGTGGC
  661 CGAATCCTTC GGGTCCCAGC AGAAACAGGA TTGGGAACAT CCCCCAGGAC ACAAAGAACA
  721 GCCAGGCCAT TCCAGTGACC ACCTGTCTAC ACCGCCCTTT AGGGACTGTG TGATATGCCT
  781 CGATGTACAC CTTTGCAGCA TTAAAGAATG TGTAGATCCC GTAGCACAGG CCCATCAGAA
  841 AGAAGATGAC GCGCACATAG CCTTTTGACA GGGCTGCTGT GGTGCCCCAG ACAATTGTTC
  901 CGATATCACT CACCAGCAGC CCCATGGTTC TCTTATTATA GTCGTTGGCC AGGCCAGTCA
  961 GGTTGCTCAG ATGAATCAGG ATCACTGGGA CAGGTCAGCA GCCCACTCAG CGTATCTCAG
 1021 CCCAGACAGT CTTATTTCCC GTTTGGAGCT ATAGATCCAC CGGGCAGGTT TCGTCAAACC
1081 TCGGTGAGTA ATTCCATGAA TTAACCTTGA TTCATTCCTC GGAATTGGTT AGGTCTCA
//
Primer: reverse RDB16750_A8Jnc.seq
>D05919A2_A8Jn_2_reverse_H02_23_ABI24.ab1
    1 CGATGGGGCT GCAGGGTCGA GGGACTCCAT AAGAGAAGAG GGACAGCTAT GACTGGGAGT
   61 AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA AGGAAAATTT TAGGGATGTT
  121 AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT AACCTCAAGT CAAGGCTTTT
  181 CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT ACTGATGACC TCTTTATAGC
  241 CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC
  301 TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA
  361 AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC
  421 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT
  481 TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
  541 GGAGTGAATT CTCATTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
  601 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC
  661 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
  721 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG
  781 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
  841 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC
  901 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG
  961 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC CTGGACGTAG CCTTCGGGCA TGGCGGGACT
 1021 TTGAAGAAGT CGTGCTGCTT CATGTGGGTC GGGGTAGCGG GCGAGCACTG CAGGCGTTAG
 1081 CCCAAGTGGT CACGAGGGTT GGGCAGCACG GCAAGCCTGG CGGTGATGCA GATCAAGCTT
1141 CAGGGAATCA GC
//
Primer: SV40pro_F_V2 RDB16750_A8Jnd.seq
>D05919A2_A8Jn_2_SV40pro_F_V2_G02_20_ABI24.ab1
    1 AAAAAACGTT TTTTTGGGAG GCCTAGGCTT TTGCAAAAGC TAACTTGTTT ATTGCAGCTT
   61 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
  121 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT
  181 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
  241 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
  301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
  361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
  421 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
  481 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
  541 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
  601 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
  661 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
  721 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
  781 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
  841 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
  901 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
  961 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAAGGA TCTCAAGAAG ATCCTTTTGA
 1021 TCTTTTTCTA CGGGGGTCTG ACGCTCAGTG GGAACGGAAA ACTTCACGGT TAGGATTTTG
 1081 GTCAATGAGG ATTATCAAAA GGATCTTCAC CTAGATTCCT TTTAAATTTT AAAAAAATTG
1141 CAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

2019.01.15

GNP_filter3_RDBDEP_html_190110.pl