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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-ChRGR-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16748
Resource name pCAGGS-ChRGR-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver (ChRGR) tagged with Venus at C-terminus, CAG promoter. Codon optimized for human.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of insert sequence LC415029 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |
Other clones in our bank

External Database
Chlamydomonas reinhardtii acop1 (channelopsin-1) Chlamydomonas reinhardtii cop4 (acop2)

        Reference sequence
          AB058890,AF385748,AB058891,AF461397

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        Additional terms and conditions for distribution Obtain written approval by Dr. Atsushi Miyawaki for Venus.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Asano, T., Front. Neurosci. 12: 561, 2018)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
        付加的提供条件 Venusについて宮脇敦史博士の書面による提供承諾を得ること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB16748 pCAGGS-ChRGR-Venus DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0087e (English text)
        Featured content Dnaconda's recommendation EXR0087j (Japanese text)
        Featured content Genetic Resources for Optgenetics Research (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16748_A8Jnp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pCAG-F
        Region: intron, insert 5'
        Sequence file: RDB16748_A8Jne.seq check
        >16748_16748_B0A9S_pCAG-F_F04_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGCGGGGTTC GGCTTCTGGC
           61 GTGTGANCGG CGGCTCTANA NNCNCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC
          121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA
          181 CCATGGCTCG GAGACCTTGG CTGCTGGCAC TGGCTCTGGC TGTCGCTCTG GCCGCTGGCT
          241 CTGCTGGGGC TTCTACTGGA TCTGACGCAA CAGTGCCCGT CGCTACTCAG GACGGACCTG
          301 ATTACGTGTT CCACAGGGCA CATGAGCGCA TGCTGTTTCA GACATCTTAT ACTCTGGAAA
          361 ACAATGGCAG TGTCATCTGC ATTCCCAACA ATGGGCAGTG CTTCTGTCTG GCATGGCTGA
          421 AGTCTAACGG CACCAATGCC GAGAAACTGG CCGCTAACAT CCTGCAGTGG ATTACATTCG
          481 CCCTGTCCGC TCTGTGCCTG ATGTTTTACG GATATCAGAC CTGGAAGTCT ACATGTGGCT
          541 GGGAGGAAAT CTACGTGGCT ACCATCGAGA TGATCAAGTT CATCATTGAA TACTTCCACG
          601 AGTTTGACGA ACCTGNCNNG ATCTATAGCT CCAACGGGAA TAAGACTGTC TGGCTGANAT
          661 NCNCTGAGTG GCTGCTGACC TGTCCAGTGA TCCTGATTCA TCTGAGCAAC CTGACTGGNC
          721 TGGCCAACGA CTATAATAAG AGAANCCATG GGGGCTGCTG GTGANTGATA TCGGAACAAT
          781 TGTCTGGGNN NNNNCANCAN NNNTNNCNAA AGGCTATGTG CGCGTCATCT TCTTTCTGAT
          841 NGGNNCTGTG CTACNGGATC TNNACATTCT TTAANGNNNN CNNAANGNGT NCNTCGAGGC
          901 NTNNNCNCNC AGTNCCTANN NNGNNNGGTG TANACNGGNN GNNCNCNNGN ANNGGCNTNN
          961 NTGNTNNTTT GNGTNCNNGG GGNANGTNNC NNNTNCNGNT TNNTGNNGGN NNCCNNANGN
        1021 ATNCNGGNNN NNTTNNNNCN NNNTNNNNTN NNNNNNNNN
        //
        Primer: Venus-N_R
        Region: Venus 5', insert 3'
        Sequence file: RDB16748_A8Jnf.seq check
        >D05919A3_A8Jn_1_Venus-N_R_B02_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTTGTGNNCG TTTNNGTCGC CGNNCNNGCT CGNNCAGNNN
           61 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC
          121 CTTCCCAGTC CCTTTGTTCA CGGCTCCTGC CTCGGCTTCA TCTTCCACCA GTGTTTCCAC
          181 CTCAATTTCG GTCCCGCCGA TATTCAGTTT GGTAGTCTTC CGAATATCGC CATGAATCAG
          241 GATATGCTCG TGGATCAGCA CTCGCAGGTA GTGTCCCAGC AGCCCCCAGC AGTTTTTGCT
          301 GGCCAGGTCC AGAATTGCAT GAGCGATGGC TGAATTAAAC TGGTTAATGT GGCCGAATCC
          361 TTCGGGTCCC AGCAGAAACA GGATTGGGAA CATCCCCCAG GACACAAAGA ACAGCCAGGC
          421 CATTCCAGTG ACCACCTGTC TACACCGCCC TTTAGGGACT GTGTGATATG CCTCGATGTA
          481 CACCTTTGCA GCATTAAAGA ATGTGTAGAT CCCGTAGCAC AGGCCCATCA GAAAGAAGAT
          541 GACGCGCACA TAGCCTTTTG ACAGGGCTGC TGTGGTGCCC CAGACAATTG TTCCGATATC
          601 ACTCACCAGC AGCCCCATGG TTCTCTTATT ATAGTCGTTG GCCAGGCCAG TCAGGTTGCT
          661 CAGATGAATC AGGATCACTG GACAGGTCAG CAGCCACTCA GCGTATCTCA GCCAGACAGT
          721 CTTATTCCCG TTGGAGCTAT AGATCACGGC AGGTTCGTCA AACTCGTGGA AGTATTCAAT
          781 GATGAACTTG ATCATCTCGA TGGTAGCCAC GTANATTTCN TCCCAGCCAC ATGTANACTT
          841 CCAGGNCTGA NATCCGTAAA ACATCANGNA CAGANCGGNN NNGGCGAATG NNNTNNNNNG
        901 CNNGNANGTT ANCNNCCANT TTNNNGNNNN NGGNNCCNNT NNGANTNNNN NNNNNN
        //
        Primer: pCAGGS_R1
        Region: b-globin p(A), Venus 3'
        Sequence file: RDB16748_A8Jng.seq check
        >16748_16748_B0A9S_pCAGGS_R1_G04_07_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNTTGNNATG ANNNNAAATT TCCTTTATTA GCCAGAAGTC
           61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
          121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
          181 ACCTGAGGAG TGAATTCTCA TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG
          241 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
          301 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG
          361 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC
          421 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
          481 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
          541 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
          601 TAGTTGCCGT CGTCCTTGAA GAAGANGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
          661 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
          721 TAGCCCAGGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
          781 AGCTTCAGGG TCAGCTTGCC GTANGTGGNA TCGCCCTCGC CCTCGCCGGA NACGCTGAAN
          841 TTGTGGNCGT TTACGTCGCC GTCCAGCTCG ANCANGANGG GCACCACCCC GGTGAANAGC
          901 TCCNCGCCCT TGNTCNNCAN GGNNNNNANC GGNNGNNNCT TNCNNGNCCC TTTGNTCNNN
          961 NNTNCNGNCN CNGCTTCNTN NNNNNNCANN GNTTCNNNCN NNNNTTCGGN CCNNNNNNNN
        1021 NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl