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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-ChRGR-Venus

Expression vector of organelle optogenetics channelrhodopsin.

Catalog number RDB16748
Resource name pCAGGS-ChRGR-Venus
Clone info. Mammarian expression vector. Channelrhodopsin-green receiver (ChRGR) tagged with Venus at C-terminus, CAG promoter. Codon optimized for human.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r (E. coli).
Gene/insert name Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of nucleotide sequence LC415029 (DDBJ accession number)
Depositor|Developer Ishizuka, Toru |
 
Other clones in our bank Chlamydomonas reinhardtii COP3 | Chlamydomonas reinhardtii COP4 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution Obtain written approval by Dr. Atsushi Miyawaki for Venus.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16748 pCAGGS-ChRGR-Venus DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0087e (English text)
Featured content Dnaconda's recommendation EXR0087j (Japanese text)
Featured content Genetic Resources for Optgenetics Research (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16748_A8Jnp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F
Region: intron, insert 5'
Sequence file: RDB16748_A8Jne.seq check
>16748_16748_B0A9S_pCAG-F_F04_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGCGGGGTTC GGCTTCTGGC
   61 GTGTGANCGG CGGCTCTANA NNCNCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC
  121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA
  181 CCATGGCTCG GAGACCTTGG CTGCTGGCAC TGGCTCTGGC TGTCGCTCTG GCCGCTGGCT
  241 CTGCTGGGGC TTCTACTGGA TCTGACGCAA CAGTGCCCGT CGCTACTCAG GACGGACCTG
  301 ATTACGTGTT CCACAGGGCA CATGAGCGCA TGCTGTTTCA GACATCTTAT ACTCTGGAAA
  361 ACAATGGCAG TGTCATCTGC ATTCCCAACA ATGGGCAGTG CTTCTGTCTG GCATGGCTGA
  421 AGTCTAACGG CACCAATGCC GAGAAACTGG CCGCTAACAT CCTGCAGTGG ATTACATTCG
  481 CCCTGTCCGC TCTGTGCCTG ATGTTTTACG GATATCAGAC CTGGAAGTCT ACATGTGGCT
  541 GGGAGGAAAT CTACGTGGCT ACCATCGAGA TGATCAAGTT CATCATTGAA TACTTCCACG
  601 AGTTTGACGA ACCTGNCNNG ATCTATAGCT CCAACGGGAA TAAGACTGTC TGGCTGANAT
  661 NCNCTGAGTG GCTGCTGACC TGTCCAGTGA TCCTGATTCA TCTGAGCAAC CTGACTGGNC
  721 TGGCCAACGA CTATAATAAG AGAANCCATG GGGGCTGCTG GTGANTGATA TCGGAACAAT
  781 TGTCTGGGNN NNNNCANCAN NNNTNNCNAA AGGCTATGTG CGCGTCATCT TCTTTCTGAT
  841 NGGNNCTGTG CTACNGGATC TNNACATTCT TTAANGNNNN CNNAANGNGT NCNTCGAGGC
  901 NTNNNCNCNC AGTNCCTANN NNGNNNGGTG TANACNGGNN GNNCNCNNGN ANNGGCNTNN
  961 NTGNTNNTTT GNGTNCNNGG GGNANGTNNC NNNTNCNGNT TNNTGNNGGN NNCCNNANGN
1021 ATNCNGGNNN NNTTNNNNCN NNNTNNNNTN NNNNNNNNN
//
Primer: Venus-N_R
Region: Venus 5', insert 3'
Sequence file: RDB16748_A8Jnf.seq check
>D05919A3_A8Jn_1_Venus-N_R_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTGTGNNCG TTTNNGTCGC CGNNCNNGCT CGNNCAGNNN
   61 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC
  121 CTTCCCAGTC CCTTTGTTCA CGGCTCCTGC CTCGGCTTCA TCTTCCACCA GTGTTTCCAC
  181 CTCAATTTCG GTCCCGCCGA TATTCAGTTT GGTAGTCTTC CGAATATCGC CATGAATCAG
  241 GATATGCTCG TGGATCAGCA CTCGCAGGTA GTGTCCCAGC AGCCCCCAGC AGTTTTTGCT
  301 GGCCAGGTCC AGAATTGCAT GAGCGATGGC TGAATTAAAC TGGTTAATGT GGCCGAATCC
  361 TTCGGGTCCC AGCAGAAACA GGATTGGGAA CATCCCCCAG GACACAAAGA ACAGCCAGGC
  421 CATTCCAGTG ACCACCTGTC TACACCGCCC TTTAGGGACT GTGTGATATG CCTCGATGTA
  481 CACCTTTGCA GCATTAAAGA ATGTGTAGAT CCCGTAGCAC AGGCCCATCA GAAAGAAGAT
  541 GACGCGCACA TAGCCTTTTG ACAGGGCTGC TGTGGTGCCC CAGACAATTG TTCCGATATC
  601 ACTCACCAGC AGCCCCATGG TTCTCTTATT ATAGTCGTTG GCCAGGCCAG TCAGGTTGCT
  661 CAGATGAATC AGGATCACTG GACAGGTCAG CAGCCACTCA GCGTATCTCA GCCAGACAGT
  721 CTTATTCCCG TTGGAGCTAT AGATCACGGC AGGTTCGTCA AACTCGTGGA AGTATTCAAT
  781 GATGAACTTG ATCATCTCGA TGGTAGCCAC GTANATTTCN TCCCAGCCAC ATGTANACTT
  841 CCAGGNCTGA NATCCGTAAA ACATCANGNA CAGANCGGNN NNGGCGAATG NNNTNNNNNG
901 CNNGNANGTT ANCNNCCANT TTNNNGNNNN NGGNNCCNNT NNGANTNNNN NNNNNN
//
Primer: pCAGGS_R1
Region: b-globin p(A), Venus 3'
Sequence file: RDB16748_A8Jng.seq check
>16748_16748_B0A9S_pCAGGS_R1_G04_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTTGNNATG ANNNNAAATT TCCTTTATTA GCCAGAAGTC
   61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG
  121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC
  181 ACCTGAGGAG TGAATTCTCA TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG
  241 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
  301 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG
  361 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC
  421 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
  481 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
  541 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
  601 TAGTTGCCGT CGTCCTTGAA GAAGANGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
  661 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
  721 TAGCCCAGGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
  781 AGCTTCAGGG TCAGCTTGCC GTANGTGGNA TCGCCCTCGC CCTCGCCGGA NACGCTGAAN
  841 TTGTGGNCGT TTACGTCGCC GTCCAGCTCG ANCANGANGG GCACCACCCC GGTGAANAGC
  901 TCCNCGCCCT TGNTCNNCAN GGNNNNNANC GGNNGNNNCT TNCNNGNCCC TTTGNTCNNN
  961 NNTNCNGNCN CNGCTTCNTN NNNNNNCANN GNTTCNNNCN NNNNTTCGGN CCNNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl