Resource data sheet
Prev.

pCALNL5:mCherry

Cre recombinase inducible expression cassette of mCherry.

Catalog number RDB16747
Resource name pCALNL5:mCherry
Clone info. Cre recombinase inducible expression cassette of mCherry.
Expression was confirmed by the depositor.
Vector backbone pCALNL5 (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor Hatanaka, Yumiko | Yamauchi, Kenta |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hatanaka, Y., Cereb. Cortex, 23 (1), 105-113, 2013) and an acknowledgment to the DEPOSITOR are requested.
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RDB16747 pCALNL5:mCherry DNA solution

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References and tips

Electronic file

Featured content

References

original Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16747_A8J9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB16747_A8J9a.seq
>D05386A1-A8J9_3_pAxCA_F1_A01_01_ABI08.ab1
    1 CCATTCCTTG CTACATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT AATTCGAGCG AACGCGTATA
  121 ACTTCGTATA GCATACATTA TACGAAGTTA TCTCGAGTCG GATTTGATCT GATCAAGAGA
  181 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
  241 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
  301 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
  361 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG
  421 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
  481 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT
  541 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
  601 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG
  661 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
  721 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
  781 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG
  841 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
  901 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA
  961 TCGCCCTTCT ATCGCCTTTC TTGACGAGTT CTTTCTGAGC GGGACTCTGG GGGTTTCGAA
 1021 ATGACCCGAA CCAGCGGACG GCCCAACCTG CCCATCACGA GGATTTCGGA TTCAACGACG
1081 GCCTATCTAT GAAGGTGGCT TTCGGATCGT TTTTCCGGGA ACGATCG
//
Primer: Neo-C_F RDB16747_A8J9b.seq
>D05386A1-A8J9_3_Neo-C_F_B01_02_ABI08.ab1
    1 GTGTGAATGG GGTAAATTGG CTGAGAGCTT GGCGGCGAAT GGGCTGAACC GCTTCCTCGG
   61 TGCTTTAACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
  121 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC
  181 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT
  241 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC
  301 ACCCCATCGA TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
  361 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
  421 TCAATGTATC TTATCATGTC TGGATCAATC CGAACGCGTA TAACTTCGTA TAGCATACAT
  481 TATACGAAGT TATCTCGAGT CGCTCGGTAC GATTTAAATT GAATTCGGCA CCATGGTGAG
  541 CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT
  601 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
  661 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
  721 GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
  781 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
  841 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
  901 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
  961 AGAAGACCAT GGGGCTGGGA GGCCCTCCCT CCGAGCGGGA TGTACCCCCG AGGACGGCGC
 1021 CCCTGAAGGG CGGAGATCAG CAGAGCTGAA AGCTGAAGGA CGCCGGCCAC TACGAACGCT
1081 GAGTCAGACA CTACCAGTCA GAGCCCGTGC CAGCCTGTCC TCC
//
Primer: mCherry-N_rev RDB16747_A8J9c.seq
>D05386A1-A8J9_3_mCherry-N_rev_C01_03_ABI08.ab1
    1 TACCCAGGAA AATTCCTTGA TGATGGGCCA TGTTATCCTC CTCGCCCTTG CTCACCATGG
   61 TGCCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA ATGTATGCTA
  121 TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA TGAGTTTGGA
  181 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT
  241 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT GGGCGAAGAA
  301 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC
  361 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG
  421 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG
  481 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG
  541 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA
  601 TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC
  661 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC
  721 ATGCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC
  781 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT
  841 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA
  901 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAA GATCCCTGCC
  961 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CCTTCCCGCT TTCAGTGACA AACGTCGAGC
 1021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC CAGCCACGAT TAGCCGGCGC TGCTCGTTCC
1081 TTGCCAGTTC AATCCAGCAA CCGAACAGGT CGGATCCTTG GACCAAAAAG GAC
//
Primer: pCA-bgter RDB16747_A8J9d.seq
>D05386A1-A8J9_3_pCA-bgter_D01_04_ABI08.ab1
    1 GCCTTAGTAA TGTCCCAATA ATTTTTGGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG
   61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
  121 GAATTGATTC CCGGGTACCT TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC
  181 CCTCGGCGCG TTCGTACTGT TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA
  241 ACTTGATGTT GACGTTGTAG GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG
  301 TCTTGACCTC AGCGTCGTAG TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC
  361 CCTTCAGGGC GCCGTCCTCG GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT
  421 TCTTCTGCAT TACGGGGCCG TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA
  481 TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA
  541 AGTTCATCAC GCGCTCCCAC TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA
  601 TGTCGGCGGG GTGCTTCACG TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT
  661 CCCAGGCGAA GGGCAGGGGG CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT
  721 CGTAGGGGCG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT
  781 CCATGTGCAC CTTGAAGCGC ATGAACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT
  841 TGCTCACCAT GGTGCCGAAT TCAATTTAAA TCGTACCGAG CGACTCGAGA ATAACTTCGT
  901 ATAATGTATG CTATACGAAG TTATACGCGT TCGGATTGAT CCAGACATGA TAAGATACAT
  961 TGATGAAGTT TGGACAAACC ACAACTAAGG AATGCAGTGA AAAAAAAATG CTTTTAATTT
 1021 GGTGGAATTT GGTGATGGCT ATTGCTTTAT TTGGTAAACC CATTTAATAA AGGCTTGCGA
 1081 TAAACCAGGT TAATTCGAAT GGGGTGGGGC CGGAGGACTC CAGCCATGAG ATCCCCTCGT
1141 CGACCTGTGG GAGGGGAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309.

2018.11.20

GNP_filter3_RDBDEP_html_181012.pl