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pCALNL5:mCherry

Cre recombinase inducible expression cassette of mCherry.

Catalog number RDB16747
Resource name pCALNL5:mCherry
Clone info. Cre recombinase inducible expression cassette of mCherry.
Comment Expression was confirmed by the depositor.
Vector backbone pCALNL5 (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Hatanaka, Yumiko | Yamauchi, Kenta |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hatanaka, Y., Cereb. Cortex, 23 (1), 105-113, 2013) and an acknowledgment to the DEPOSITOR are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hatanaka, Y., Cereb. Cortex, 23 (1), 105-113, 2013) を引用し、謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16747 pCALNL5:mCherry DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0086e (English text)
            Featured content Dnaconda's recommendation EXR0086j (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16747_A8J9p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB16747_A8J9a.seq check
            >D05386A1-A8J9_3_pAxCA_F1_A01_01_ABI08.ab1
                1 CCATTCCTTG CTACATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
               61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT AATTCGAGCG AACGCGTATA
              121 ACTTCGTATA GCATACATTA TACGAAGTTA TCTCGAGTCG GATTTGATCT GATCAAGAGA
              181 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
              241 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
              301 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
              361 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG
              421 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
              481 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT
              541 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
              601 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG
              661 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
              721 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
              781 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG
              841 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
              901 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA
              961 TCGCCCTTCT ATCGCCTTTC TTGACGAGTT CTTTCTGAGC GGGACTCTGG GGGTTTCGAA
             1021 ATGACCCGAA CCAGCGGACG GCCCAACCTG CCCATCACGA GGATTTCGGA TTCAACGACG
            1081 GCCTATCTAT GAAGGTGGCT TTCGGATCGT TTTTCCGGGA ACGATCG
            //
            Primer: Neo-C_F
            Sequence file: RDB16747_A8J9b.seq check
            >D05386A1-A8J9_3_Neo-C_F_B01_02_ABI08.ab1
                1 GTGTGAATGG GGTAAATTGG CTGAGAGCTT GGCGGCGAAT GGGCTGAACC GCTTCCTCGG
               61 TGCTTTAACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
              121 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC
              181 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT
              241 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC
              301 ACCCCATCGA TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
              361 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
              421 TCAATGTATC TTATCATGTC TGGATCAATC CGAACGCGTA TAACTTCGTA TAGCATACAT
              481 TATACGAAGT TATCTCGAGT CGCTCGGTAC GATTTAAATT GAATTCGGCA CCATGGTGAG
              541 CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT
              601 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
              661 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
              721 GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
              781 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
              841 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
              901 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
              961 AGAAGACCAT GGGGCTGGGA GGCCCTCCCT CCGAGCGGGA TGTACCCCCG AGGACGGCGC
             1021 CCCTGAAGGG CGGAGATCAG CAGAGCTGAA AGCTGAAGGA CGCCGGCCAC TACGAACGCT
            1081 GAGTCAGACA CTACCAGTCA GAGCCCGTGC CAGCCTGTCC TCC
            //
            Primer: mCherry-N_rev
            Sequence file: RDB16747_A8J9c.seq check
            >D05386A1-A8J9_3_mCherry-N_rev_C01_03_ABI08.ab1
                1 TACCCAGGAA AATTCCTTGA TGATGGGCCA TGTTATCCTC CTCGCCCTTG CTCACCATGG
               61 TGCCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA ATGTATGCTA
              121 TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA TGAGTTTGGA
              181 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT
              241 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT GGGCGAAGAA
              301 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC
              361 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG
              421 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG
              481 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG
              541 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA
              601 TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC
              661 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC
              721 ATGCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC
              781 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT
              841 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA
              901 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAA GATCCCTGCC
              961 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CCTTCCCGCT TTCAGTGACA AACGTCGAGC
             1021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC CAGCCACGAT TAGCCGGCGC TGCTCGTTCC
            1081 TTGCCAGTTC AATCCAGCAA CCGAACAGGT CGGATCCTTG GACCAAAAAG GAC
            //
            Primer: pCA-bgter
            Sequence file: RDB16747_A8J9d.seq check
            >D05386A1-A8J9_3_pCA-bgter_D01_04_ABI08.ab1
                1 GCCTTAGTAA TGTCCCAATA ATTTTTGGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG
               61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
              121 GAATTGATTC CCGGGTACCT TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC
              181 CCTCGGCGCG TTCGTACTGT TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA
              241 ACTTGATGTT GACGTTGTAG GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG
              301 TCTTGACCTC AGCGTCGTAG TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC
              361 CCTTCAGGGC GCCGTCCTCG GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT
              421 TCTTCTGCAT TACGGGGCCG TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA
              481 TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA
              541 AGTTCATCAC GCGCTCCCAC TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA
              601 TGTCGGCGGG GTGCTTCACG TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT
              661 CCCAGGCGAA GGGCAGGGGG CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT
              721 CGTAGGGGCG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT
              781 CCATGTGCAC CTTGAAGCGC ATGAACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT
              841 TGCTCACCAT GGTGCCGAAT TCAATTTAAA TCGTACCGAG CGACTCGAGA ATAACTTCGT
              901 ATAATGTATG CTATACGAAG TTATACGCGT TCGGATTGAT CCAGACATGA TAAGATACAT
              961 TGATGAAGTT TGGACAAACC ACAACTAAGG AATGCAGTGA AAAAAAAATG CTTTTAATTT
             1021 GGTGGAATTT GGTGATGGCT ATTGCTTTAT TTGGTAAACC CATTTAATAA AGGCTTGCGA
             1081 TAAACCAGGT TAATTCGAAT GGGGTGGGGC CGGAGGACTC CAGCCATGAG ATCCCCTCGT
            1141 CGACCTGTGG GAGGGGAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309.
            reference Kita, Y., Specific labeling of climbing fibers shows early synaptic interactions with immature Purkinje cells in the prenatal cerebellum. Dev. Neurobiol. 75 (9): 927-934 (2015). PMID 25529108.
            Hatanaka, Y., Cortical Divergent Projections in Mice Originate from Two Sequentially Generated, Distinct Populations of Excitatory Cortical Neurons with Different Initial Axonal Outgrowth Characteristics. Cereb. Cortex. 26 (5): 2257-2270 (2016). PMID 25882037.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl