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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCALNL5:mCherry (#RDB16747)

Cre recombinase inducible expression cassette of mCherry.

Clone info. Cre recombinase inducible expression cassette of mCherry.
Comment Expression was confirmed by the depositor.
Vector backbone pCALNL5 (RDB01862) (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Hatanaka, Yumiko | Yamauchi, Kenta |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hatanaka, Y., Cereb. Cortex, 23 (1), 105-113, 2013) and an acknowledgment to the DEPOSITOR are requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hatanaka, Y., Cereb. Cortex, 23 (1), 105-113, 2013) を引用し、謝辞の表明を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16747 pCALNL5:mCherry DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCALNL5:mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16747).

Reference section:

Hatanaka, Y., Yamauchi, K.., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16747_A8J9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB16747_A8J9a.seq check
>D05386A1-A8J9_3_pAxCA_F1_A01_01_ABI08.ab1
    1 CCATTCCTTG CTACATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT AATTCGAGCG AACGCGTATA
  121 ACTTCGTATA GCATACATTA TACGAAGTTA TCTCGAGTCG GATTTGATCT GATCAAGAGA
  181 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
  241 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
  301 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
  361 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG
  421 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
  481 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT
  541 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
  601 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG
  661 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
  721 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
  781 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG
  841 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
  901 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA
  961 TCGCCCTTCT ATCGCCTTTC TTGACGAGTT CTTTCTGAGC GGGACTCTGG GGGTTTCGAA
 1021 ATGACCCGAA CCAGCGGACG GCCCAACCTG CCCATCACGA GGATTTCGGA TTCAACGACG
1081 GCCTATCTAT GAAGGTGGCT TTCGGATCGT TTTTCCGGGA ACGATCG
//
Primer: Neo-C_F
Sequence file: RDB16747_A8J9b.seq check
>D05386A1-A8J9_3_Neo-C_F_B01_02_ABI08.ab1
    1 GTGTGAATGG GGTAAATTGG CTGAGAGCTT GGCGGCGAAT GGGCTGAACC GCTTCCTCGG
   61 TGCTTTAACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
  121 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC
  181 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT
  241 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC
  301 ACCCCATCGA TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
  361 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
  421 TCAATGTATC TTATCATGTC TGGATCAATC CGAACGCGTA TAACTTCGTA TAGCATACAT
  481 TATACGAAGT TATCTCGAGT CGCTCGGTAC GATTTAAATT GAATTCGGCA CCATGGTGAG
  541 CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT
  601 GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA
  661 CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG
  721 GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA
  781 CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA
  841 CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT
  901 CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA
  961 AGAAGACCAT GGGGCTGGGA GGCCCTCCCT CCGAGCGGGA TGTACCCCCG AGGACGGCGC
 1021 CCCTGAAGGG CGGAGATCAG CAGAGCTGAA AGCTGAAGGA CGCCGGCCAC TACGAACGCT
1081 GAGTCAGACA CTACCAGTCA GAGCCCGTGC CAGCCTGTCC TCC
//
Primer: mCherry-N_rev
Sequence file: RDB16747_A8J9c.seq check
>D05386A1-A8J9_3_mCherry-N_rev_C01_03_ABI08.ab1
    1 TACCCAGGAA AATTCCTTGA TGATGGGCCA TGTTATCCTC CTCGCCCTTG CTCACCATGG
   61 TGCCGAATTC AATTTAAATC GTACCGAGCG ACTCGAGATA ACTTCGTATA ATGTATGCTA
  121 TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA GATACATTGA TGAGTTTGGA
  181 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT
  241 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCGATGGGGT GGGCGAAGAA
  301 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC
  361 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG
  421 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG
  481 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG
  541 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA
  601 TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC
  661 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC
  721 ATGCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC
  781 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT
  841 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA
  901 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAA GATCCCTGCC
  961 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CCTTCCCGCT TTCAGTGACA AACGTCGAGC
 1021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC CAGCCACGAT TAGCCGGCGC TGCTCGTTCC
1081 TTGCCAGTTC AATCCAGCAA CCGAACAGGT CGGATCCTTG GACCAAAAAG GAC
//
Primer: pCA-bgter
Sequence file: RDB16747_A8J9d.seq check
>D05386A1-A8J9_3_pCA-bgter_D01_04_ABI08.ab1
    1 GCCTTAGTAA TGTCCCAATA ATTTTTGGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG
   61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
  121 GAATTGATTC CCGGGTACCT TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC
  181 CCTCGGCGCG TTCGTACTGT TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA
  241 ACTTGATGTT GACGTTGTAG GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG
  301 TCTTGACCTC AGCGTCGTAG TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC
  361 CCTTCAGGGC GCCGTCCTCG GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT
  421 TCTTCTGCAT TACGGGGCCG TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA
  481 TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA
  541 AGTTCATCAC GCGCTCCCAC TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA
  601 TGTCGGCGGG GTGCTTCACG TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT
  661 CCCAGGCGAA GGGCAGGGGG CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT
  721 CGTAGGGGCG GCCCTCGCCC TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT
  781 CCATGTGCAC CTTGAAGCGC ATGAACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT
  841 TGCTCACCAT GGTGCCGAAT TCAATTTAAA TCGTACCGAG CGACTCGAGA ATAACTTCGT
  901 ATAATGTATG CTATACGAAG TTATACGCGT TCGGATTGAT CCAGACATGA TAAGATACAT
  961 TGATGAAGTT TGGACAAACC ACAACTAAGG AATGCAGTGA AAAAAAAATG CTTTTAATTT
 1021 GGTGGAATTT GGTGATGGCT ATTGCTTTAT TTGGTAAACC CATTTAATAA AGGCTTGCGA
 1081 TAAACCAGGT TAATTCGAAT GGGGTGGGGC CGGAGGACTC CAGCCATGAG ATCCCCTCGT
1141 CGACCTGTGG GAGGGGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hatanaka, Y., Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cereb. Cortex, 23 (1): 105-113 (2013). PMID 22267309. [link to RRC of NBRP]
reference Kita, Y., Specific labeling of climbing fibers shows early synaptic interactions with immature Purkinje cells in the prenatal cerebellum. Dev. Neurobiol. 75 (9): 927-934 (2015). PMID 25529108.
未 Hatanaka, Y., Cortical Divergent Projections in Mice Originate from Two Sequentially Generated, Distinct Populations of Excitatory Cortical Neurons with Different Initial Axonal Outgrowth Characteristics. Cereb. Cortex. 26 (5): 2257-2270 (2016). PMID 25882037.