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pGL3-mouse RORgammat promoter (-710 to +68)

Luciferase reporter of the mouse retinoic acid-related orphan receptor gamma t (ROR gamma t) promoter (-710 to +68).

Catalog number RDB16746
Resource name pGL3-mouse RORgammat promoter (-710 to +68)
Clone info. Luciferase reporter of the mouse retinoic acid-related orphan receptor gamma t (ROR gamma t) promoter (-710 to +68).
Vector backbone pGL3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rorc genomic DNA
Depositor|Developer Maruyama, Takashi |
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External Database
mouse Rorc

          Reference sequence
            
           
          Sequence (full) RDB16746zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB16746 pGL3-mouse RORgammat promoter (-710 to +68) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL3-mouse RORgammat promoter (-710 to +68) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16746).

          Reference section:

          ,

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16746_A8Ipp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: synthPA_F (Pr0257)
          Region: Pause site,insert 5'
          Sequence file: RDB16746_A8Ipa.seq check
          >D05839D2_A8Ip_2_synthPA_F_A03_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT ACGCTCTCNN TCNAAACNAA ACGAAACAAA ACAAACTAGC
             61 AAAATAGGCT GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCTATC GATAGGTACC
            121 GAGCTCTCAA CTTGTTGGTC CAGTGCAGTT GCTCTCTTCT GCTCTCCCTC CAACACCCCT
            181 CACCTCTCAA TTTGCTCTTC CTCCTCCACA CCTCCCCCCA CCACACCAGA AGTATGGGAA
            241 GCATCTAGAA GCCTGGAAAA TCCAGAGGTG GGCAGCATGG AGTTAGAGAG AGGCAAAGCA
            301 AGACATGTGC TGGGGGAACC CAAGCTGCAG GGAAGTATAT CTGATCCCAT CCTGGAGATC
            361 AGAGTGCACA CACACACACA CACACACACA CACAGAGGCT GTCTTTTCTC TTCACTCTTC
            421 CTGCCTCCCT TTCTTGCTAT TCTGTCACCT GTCCTAAAAA AGTTACAGAG ACCAGGTTCA
            481 AATCTCAACT CAATCACGAA TTGCCCTTGT GAATTCCTGG CAAGTCTTTT CTATTCTGGG
            541 CAGCAATTGG TTCATGATTA TGAATAATCA CTCGTGCCTG TAGGGGGTTA TCATGTGGGA
            601 TTCCAAAAAA CAAACAAAAA AATTCTGATT TACCTACAAG GCCTGGCAAA AACTCAGCAT
            661 ACAGTAGGTA ATTAATAAGT ACTAGCTCCC TTTCACCTGT TTTTCCTCAA GACTCCAGAC
            721 ACTGGTCCAC TGCTCTCTCC CTGANTCCCC TCCTGTGCCA GGACACTCTG CCAGCCACTC
            781 CTTTCCCCTG CCTGCTGACG GGCCANGTGC TCCCTCCCTC TTCCCTCCTC CCTCCNNTGN
            841 GCCCTGCTCC CNGCNNTCCT GGGGCAGCCA GGGCAGCAAG GACGGCACCN ANGGANCTAC
            901 CCNNTAGATC TGCNATCTAN NTAANCTNNN CNNNNCGGNA NNGNNGGNNN NNCCNCCNNG
          961 GNANANGCCN AAAACNTAAA NNNNNCCCNN NNNNNNNN
          //
          Primer: Luc_R (Pr0146)
          Region: Luc_R,insert 3'
          Sequence file: RDB16746_A8Ipb.seq check
          >16746_16746_B1EqF_2_Luc_R_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT CTTNNNNNNC TTATGNNNGT TGCTCTCCNG
             61 CGGTTCCNTC TTNNNNNGGA TAGAATGNNN NNNNNGGCCT TTCTTTATGT TTTTGGCGTC
            121 TTCCATGGTG GCTTTACCAA CAGTACCGGA ATGCCAAGCT TACTTAGATC GCAGATCTAT
            181 GGGGTAGCTC CCTTGGTGCC GTCCTTGCTG CCCTGGCTGC CCAGGAGGGC AGGGAGCAGG
            241 GCCCAAGGGA GGGAGGAGGG AAGAGGGAGG GAGCACCTGG CCCGTCAGCA GGCAGGGGAA
            301 AGGAGTGGCT GGCAGAGTGT CCTGGCACAG GAGGGGACTC AGGGAGAGAG CAGTGGACCA
            361 GTGTCTGGAG TCTTGAGGAA AAACAGGTGA AAGGGAGCTA GTACTTATTA ATTACCTACT
            421 GTATGCTGAG TTTTTGCCAG GCCTTGTAGG TAAATCAGAA TTTTTTTGTT TGTTTTTTGG
            481 AATCCCACAT GATAACCCCC TACAGGCACG AGTGATTATT CATAATCATG AACCAATTGC
            541 TGCCCAGAAT AGAAAAGACT TGCCAGGAAT TCACNAGGGC AATTCGTGAT TGNNNNNNNA
            601 NTTGAACCTG GTCTCTGTAA CTTTTTTAGG ACANGTGACA GAATANCAAG AAAGGGANGC
            661 AGGAANAGTG NAGAGAAAAG ACAGCCTCTG NGTGTGTGTG TGTGTGNGTG TGTGNNNNNN
          721 NNNN
          //
          Primer: SV40pA_R (Pr0882)
          Region: SV40 pA,Luc 3'
          Sequence file: RDB16746_A8Ipc.seq check
          >16746_16746_B1EqF_2_SV40pA_R_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT TNNNNAATAN NNNNNTTTTT TNNCTGCATT CTAGTTGTGG
             61 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC
            121 TCTAGAATTA CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT
            181 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC
            241 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG
            301 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT
            361 CGGGAAGACC TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA
            421 ATTCAGCGGG AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA
            481 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT
            541 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAN
            601 CTCTCACACA CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGNA TCCAGATCCN
            661 CAACCTTCNN TTCNAAAAAN GNNNNNNCTT TANCGANCNN GCCCNGNNNN NNCNNNCCCN
          721 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original unpublished,

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl