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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX6P-TDP43WT-6xHis

Expression vector of human TAR DNA-binding protein 43 (TDP43).

Catalog number RDB16710
Resource name pGEX6P-TDP43WT-6xHis
Clone info. Expression vector of human TAR DNA-binding protein 43 (TDP43), wild type, fused with GST at N-terminal and 6×His at C-terminal. For the constitution, please refer Fig. 1A of Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018.
Comment Expression was confirmed by the deposior with SDS-PAGE and silver staining.
Vector backbone pGEX-6P-1 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human TARDBP cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human TARDBP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)。
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          RDB16710 pGEX6P-TDP43WT-6xHis DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16710_A8Ilp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB16710_A8Ila.seq check
          >D05635A4-3_A8Il_pGEX-F2_B02_05_ABI24.ab1
              1 GACTAAAATT TAGTTTTCTT TTAAAAACGT ATTGGAAGCT ATCCCACAAA TTGATAAGTA
             61 CTTGAAATCC AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG
            121 TGGCGACCAT CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCAT
            181 GTCTGAATAT ATTCGGGTAA CCGAAGATGA GAACGATGAG CCCATTGAAA TACCATCGGA
            241 AGACGATGGG ACGGTGCTGC TCTCCACGGT TACAGCCCAG TTTCCAGGGG CGTGTGGGCT
            301 TCGCTACAGG AATCCAGTGT CTCAGTGTAT GAGAGGTGTC CGGCTGGTAG AAGGAATTCT
            361 GCATGCCCCA GATGCTGGCT GGGGAAATCT GGTGTATGTT GTCAACTATC CAAAAGATAA
            421 CAAAAGAAAA ATGGATGAGA CAGATGCTTC ATCAGCAGTG AAAGTGAAAA GAGCAGTCCA
            481 GAAAACATCC GATTTAATAG TGTTGGGTCT CCCATGGAAA ACAACCGAAC AGGACCTGAA
            541 AGAGTATTTT AGTACCTTTG GAGAAGTTCT TATGGTGCAG GTCAAGAAAG ATCTTAAGAC
            601 TGGTCATTCA AAGGGGTTTG GCTTTGTTCG TTTTACGGAA TATGAAACAC AAGTGAAAGT
            661 AATGTCACAG CGACATATGA TAGATGGACG ATGGTGTGAC TGCAAACTTC CTAATTCTAA
            721 GCAAAGCCAA GATGAGCCTT TGAGAAGCAG AAAAGTGTTT GTGGGGCGCT GTACAGAGGA
            781 CATGACTGAG GATGAGCTGC GGGAGTTCTT CTCTCAGTAC GGGGATGTGA TGGATGTCTT
            841 CATCCCCAAG CCATTCAGGG CCTTTGCCTT TGTTACATTT GCAGATGATC AGATTGCGCA
            901 GTCTCTTTGT GGAGAGGACT TGATCATTAA AGGAATCAGC GTTCATATAT CCAATGCCGA
            961 ACCTAAGCAC AATAGCAATA GACAGTTAGA AAGAAGTGGA AAGATTTGGG TGGTAATCCA
           1021 GGTGGCTTTG GGAATCAAGG GTGGAATTTG GGTAATAAGC AGAGGGGGTG GAGCTGGTTG
           1081 GGAAACAATC AAGTAGTAAT ATGGGTGGTG GGAATGAACC TTTGGTTGCG TCAGGCATTA
           1141 ATCCCAGCAT GATGGCCTGC CGGCCCAAGC AGCACTACGG ACCGATGGGG TACTAGCTGC
          1201 ATG
          //
          Primer: pJB8-Forward
          Sequence file: RDB16710_A8Ilb.seq check
          >D05635A4-3_A8Il_pJB8-Forward_C02_08_ABI24.ab1
              1 ACCGCCAAAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT
             61 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC
            121 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGATC
            181 GTCAGTCAGT CACGATGCGG CCGCTCGAGT CGACTTAATG GTGGTGATGG TGGTGATTCA
            241 TTCCCCAGCC AGAAGACTTA GAATCCATGC TTGAGCCAAA GCCTCCATTA AAACCACTGC
            301 CCGACCCTGC ATTGGATGCT GATCCCCAAC CAATTGCTGC ACCAGAATTA GAGCCACTAT
            361 AAGAGTTATT TCCAGAACCG AAGGCCTGGT TTGGCTCCCT CTGCATGTTG CCTTGGTTTT
            421 GGTTATTACC CGATGGGCCT GACTGGTTCT GCTGGCTGGC TAACATGCCC ATCATACCCC
            481 AACTGCTCTG TAGTGCTGCC TGGGCGGCAG CCATCATGGC TGGATTAATG CTGAACGCAC
            541 CAAAGTTCAT CCCACCACCC ATATTACTAC CTTGATTGTT TCCCAAACCA GCTCCACCCC
            601 CTCTGCTATT ACCAAATCCA CCCTGATTCC CAAAGCCACC TGGATTACCA CCAAATCTTC
            661 CACTTCTTTC TAACTGTCTA TTGCTATTGT GCTTAGGTTC GGCATTGGAT ATATGAACGC
            721 TGATTCCTTT AATGATCAAG TCCTCTCCAC AAAGAGACTG CGCAATCTGA TCATCTGCAA
            781 ATGTAACAAA GGCAAAGGCC CTGAATGGCT TGGGGATGAA GACATCCATC ACATCCCCGT
            841 ACTGAGAGAA GAACTCCCGC AGCTCATCCT CAGTCATGTC CTCTGTACAG CGCCCCACAA
            901 ACACTTTTCT GCTTCTCAAA GGCTCATCTT GGCTTTGCTT AGAATTAGGA AGTTTGCAGT
            961 CACACCATCG TCCATCTATC ATATGTCGCT GTGACATTAC TTTCACTTGT GTTTCATATT
           1021 CCGTAAAACG ACAAAGCCAA ACCCCTTTGA TGACCAGTCT TAGATCTTTT CTTGACCTGC
           1081 ACCATAGAAC TTCTCAACGT ACTAAAATAC TCTTCAAGTC TGGTCCGGTT GTTTTTCATG
          1141 GGAGAACCAA CACTAATTAA AATTCGGGAG AGTTAAT
          //
          Primer: M13_-40
          Sequence file: RDB16710_A8Ilc.seq check
          >D05635A4-3_A8Il_M13_-40_D02_11_ABI24.ab1
              1 TAAGGCAAGG TGAAATCCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
             61 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
            121 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
            181 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
            241 TGGGCGCTAG CGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA
            301 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA
            361 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT
            421 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG
            481 CGCCCAGCGC CATCTGATCG TTGGCAAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC
            541 AGCAATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC
            601 TATCGGCTGA AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
            661 CCGAGACAGG ACTTAATGGG CCCGGCTAAC AGCGCGATTT GCTGGTGGAC CCAATGCGAC
            721 CAGATGCTCA GGCCAGTCGC GTACCGTCTT CTGGGAAGAA AATAATACTG TTGATGGGTG
            781 TCTGGTCAGA GACATCAGAA AATAACGCCG GACATTATTG CAGCAGCTTC CACACATGGC
            841 ATTCTGTCTA TCCAACCGGA TAAGTTAATG ATCACCCACT GACCGTTGCC CGAAAAATTT
            901 GGATACGCCC TTTCAGGTTT GAAGCGCCTT CGTTTTTACT CTGAACCACC AGCTGGGACC
            961 CAGATTTATA TGGGCAGAAT TTATACTCCA CAATTGCCAG CCGTTCGAGG GCCCGACTGA
           1021 GTGGAGACCG ATCCACACGC ATGATCGCCC ACGTTGTTGT CGCGCGTGAA GATATAAATT
          1081 CGACGACCGG TTACTATTCG TTCTAAACAG GATGCGTCGT AC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl