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GFP-OPTN-E478G

Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E478G mutant.

Catalog number RDB16709
Resource name GFP-OPTN-E478G
Clone info. Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E478G mutant, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human OPTN

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB16709 GFP-OPTN-E478G DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16709_A8Ilp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16709_A8Ila.seq check
          >D05635A3-3_A8Il_CMV-Forward_G01_19_ABI24.ab1
              1 TTGGTACACT CCGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
            121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
            181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
            241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
            301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
            361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
            421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
            481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
            541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
            601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
            661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
            721 AGCACCCAGT CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
            781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
            841 AGATCTCGAG CTCAAGCTTC GAATTCTATG TCCCATCAAC CTCTCAGCTG CCTCACTGAA
            901 AAGGAGGACA GCCCCAGTGA AAGCACAGGA AATGGACCCC CCCCACCTGG CCCACCCAAA
            961 CCTGGACACG TTTACCCCGG GAGGAGCTGC TGCAGCAGAT GAAAGAGCTC CCTGACCGAG
           1021 AACCACCAGC TGAAAGAAGC CATGAAGCTA AATAATCAGC CCATGAAAGG AGATTTGAGA
           1081 GCTTTCGGCC TGACAGAGAA AACAGGGAGA ACGGCAGGTT TTTGAGATAC AGAGCAAGAG
          1141 GCAAAAGGAG CGCGTCCTTA ATTG
          //
          Primer: SV40polyA-R
          Sequence file: RDB16709_A8Ilb.seq check
          >D05635A3-3_A8Il_SV40polyA-R_H01_22_ABI24.ab1
              1 AAAAATATTG GGAAGTTTTT TTAAAGAAGG TAAAACCTCT AAAATGTGGG TATGGCTGAT
             61 TATGATCAGT TATCTAGATC CGGTGGATCC CGAATGATGC AATCCATCAC GTGAATCTGT
            121 AACGTGTCTA TGTCAGGCAG AACCTCTCCA CACTTGGGGC AGGAATGAAT CGGAATATTC
            181 CGCTGTTGCC GCCAGTCCCT GTCCTCAGCT CCTCTTTGAA CAAGGTAAGC CTGCTGGTCA
            241 GAGTCACTTG TTCTCGCCCC ATGACGACTC TGCATCTCCA TCAAGGACTG CCTGCCTCCG
            301 TCTTCGAAAG CATCATTCTC TTTCAGCAGA ACTGCCAGCT GCAATGCCAG TTGCTCCTTT
            361 TCCTCATGAA TTTTCTCTCT CGCTGCTCTT CCAGCATGAA AATCAGAACA GTAAACTTCC
            421 ATCTGAGCCC TGAGGATGGT CATGGTTTCC AGGTCCTCTT CCTGCTTGGC AATGGTTTGC
            481 TTCATTTCAT CCATTTGCAG CTGTTTGGAA GCCAGAGCCT TCTCTGCCAG TTCCAGTTTT
            541 TCACTCAGTT CCTTCAGCAC TGCCCTGTCC ACTTTTTCTG ACTCTTTTCT TGTTAGTTCC
            601 TCAATTGTTT TCAATGCATT ATTATGTTCT TGAAGAAGCT TGTTGTGTGT CATCTGTAGC
            661 ACAGTTAATT TGGACTTTTC ATCCTCTGTT TTAGCCTGTT CCATTTTGAT TTCTGATAAG
            721 CATGCTTTCC ACTTGTAGCT CTAAACTTTT TGTTAGTATA AACAAGGCTC TTGCTTTTCA
            781 TTTCAACTCT GATGGAATTG CAGAATTTTT TCCCTTTTCA AGGGCCTGAC ACTTTTCTTG
            841 AAGTCTCTTC TTCATTAGCT CAGCTTCGCT GAGTTTTGTA TGAAGCCTCT TGAAGCTCCT
            901 TAAACAAAGA TGTCACCCTG GAGGGTTCAA GTGCTTCCAC TTCGCTTCCA ACAGTCTCCG
            961 GGCCTTTCTC TTCATCATTC TCTTTCTCTG TGCTCCCCTC GGTCTGGGTT CAATCCTCAG
           1021 GACGATTACT TGTTTCCTTT TCAAAATCTT GGACTTCTTT CTTGGCCTGC CTTGAATGGC
           1081 AACTTCAAGT TCCTCTCCGA CCGTTCGGAA GTCGCTTCCT TTAGGCAAAG CAAGGGGAGC
           1141 CTTGGGCTTC ACGATTACTT CCCTTCATGT TCAAGTTAAT CCGTTGGGCT CCCACGTTCT
          1201 TGTGCCAAAA GATTACTGGC CCCTGTCCAT TAATTAT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB16709_A8Ilc.seq check
          >D05635A3-3_A8Il_SV40pro_F_V2_A02_02_ABI24.ab1
              1 GAGGGAAGCT TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG
             61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
            601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
            781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
            841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
            901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
            961 TTCGGAATCG TTTTCCGGGA CGCCGGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
           1021 GGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG
           1081 GAGGGACCCG CGCTATGACG CATAAAGACG GATAAACGCA CGGTGTTGGG TCGTTGTCAT
          1141 TAACGCGGGC TTCGCTCAGG CTGCACTCTG TTTCGCGAAT ATATGTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl