Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GFP-OPTN-E478G (#RDB16709)

Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E478G mutant.

Clone info. Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E478G mutant, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16709 GFP-OPTN-E478G DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GFP-OPTN-E478G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16709).

Reference section:

Kitamura, A., Shimizu, H., Kinjo, M., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16709_A8Ilp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB16709_A8Ila.seq check
>D05635A3-3_A8Il_CMV-Forward_G01_19_ABI24.ab1
    1 TTGGTACACT CCGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
  841 AGATCTCGAG CTCAAGCTTC GAATTCTATG TCCCATCAAC CTCTCAGCTG CCTCACTGAA
  901 AAGGAGGACA GCCCCAGTGA AAGCACAGGA AATGGACCCC CCCCACCTGG CCCACCCAAA
  961 CCTGGACACG TTTACCCCGG GAGGAGCTGC TGCAGCAGAT GAAAGAGCTC CCTGACCGAG
 1021 AACCACCAGC TGAAAGAAGC CATGAAGCTA AATAATCAGC CCATGAAAGG AGATTTGAGA
 1081 GCTTTCGGCC TGACAGAGAA AACAGGGAGA ACGGCAGGTT TTTGAGATAC AGAGCAAGAG
1141 GCAAAAGGAG CGCGTCCTTA ATTG
//
Primer: SV40polyA-R ()
Sequence file: RDB16709_A8Ilb.seq check
>D05635A3-3_A8Il_SV40polyA-R_H01_22_ABI24.ab1
    1 AAAAATATTG GGAAGTTTTT TTAAAGAAGG TAAAACCTCT AAAATGTGGG TATGGCTGAT
   61 TATGATCAGT TATCTAGATC CGGTGGATCC CGAATGATGC AATCCATCAC GTGAATCTGT
  121 AACGTGTCTA TGTCAGGCAG AACCTCTCCA CACTTGGGGC AGGAATGAAT CGGAATATTC
  181 CGCTGTTGCC GCCAGTCCCT GTCCTCAGCT CCTCTTTGAA CAAGGTAAGC CTGCTGGTCA
  241 GAGTCACTTG TTCTCGCCCC ATGACGACTC TGCATCTCCA TCAAGGACTG CCTGCCTCCG
  301 TCTTCGAAAG CATCATTCTC TTTCAGCAGA ACTGCCAGCT GCAATGCCAG TTGCTCCTTT
  361 TCCTCATGAA TTTTCTCTCT CGCTGCTCTT CCAGCATGAA AATCAGAACA GTAAACTTCC
  421 ATCTGAGCCC TGAGGATGGT CATGGTTTCC AGGTCCTCTT CCTGCTTGGC AATGGTTTGC
  481 TTCATTTCAT CCATTTGCAG CTGTTTGGAA GCCAGAGCCT TCTCTGCCAG TTCCAGTTTT
  541 TCACTCAGTT CCTTCAGCAC TGCCCTGTCC ACTTTTTCTG ACTCTTTTCT TGTTAGTTCC
  601 TCAATTGTTT TCAATGCATT ATTATGTTCT TGAAGAAGCT TGTTGTGTGT CATCTGTAGC
  661 ACAGTTAATT TGGACTTTTC ATCCTCTGTT TTAGCCTGTT CCATTTTGAT TTCTGATAAG
  721 CATGCTTTCC ACTTGTAGCT CTAAACTTTT TGTTAGTATA AACAAGGCTC TTGCTTTTCA
  781 TTTCAACTCT GATGGAATTG CAGAATTTTT TCCCTTTTCA AGGGCCTGAC ACTTTTCTTG
  841 AAGTCTCTTC TTCATTAGCT CAGCTTCGCT GAGTTTTGTA TGAAGCCTCT TGAAGCTCCT
  901 TAAACAAAGA TGTCACCCTG GAGGGTTCAA GTGCTTCCAC TTCGCTTCCA ACAGTCTCCG
  961 GGCCTTTCTC TTCATCATTC TCTTTCTCTG TGCTCCCCTC GGTCTGGGTT CAATCCTCAG
 1021 GACGATTACT TGTTTCCTTT TCAAAATCTT GGACTTCTTT CTTGGCCTGC CTTGAATGGC
 1081 AACTTCAAGT TCCTCTCCGA CCGTTCGGAA GTCGCTTCCT TTAGGCAAAG CAAGGGGAGC
 1141 CTTGGGCTTC ACGATTACTT CCCTTCATGT TCAAGTTAAT CCGTTGGGCT CCCACGTTCT
1201 TGTGCCAAAA GATTACTGGC CCCTGTCCAT TAATTAT
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB16709_A8Ilc.seq check
>D05635A3-3_A8Il_SV40pro_F_V2_A02_02_ABI24.ab1
    1 GAGGGAAGCT TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG
   61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  961 TTCGGAATCG TTTTCCGGGA CGCCGGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
 1021 GGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG
 1081 GAGGGACCCG CGCTATGACG CATAAAGACG GATAAACGCA CGGTGTTGGG TCGTTGTCAT
1141 TAACGCGGGC TTCGCTCAGG CTGCACTCTG TTTCGCGAAT ATATGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]