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GFP-OPTN-E50K

Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant.

Catalog number RDB16708
Resource name GFP-OPTN-E50K
Clone info. Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human OPTN

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB16708 GFP-OPTN-E50K DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16708_A8Ilp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: VenusC_F2
          Sequence file: RDB16708_A8Ila.seq check
          >D05635A2_A8Il__1_VenusC_F2_B01_02_ABI08.ab1
              1 GGGAGTGGTA AAGCGCCGGG AACACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
             61 GATCTCGAGC TCAAGCTTCG AATTCTATGT CCCATCAACC TCTCAGCTGC CTCACTGAAA
            121 AGGAGGACAG CCCCAGTGAA AGCACAGGAA ATGGACCCCC CCACCTGGCC CACCCAAACC
            181 TGGACACGTT TACCCCGGAG GAGCTGCTGC AGCAGATGAA AGAGCTCCTG ACCAAGAACC
            241 ACCAGCTGAA AGAAGCCATG AAGCTAAATA ATCAAGCCAT GAAAGGGAGA TTTGAGGAGC
            301 TTTCGGCCTG GACAGAGAAA CAGAAGGAAG AACGCCAGTT TTTTGAGATA CAGAGCAAAG
            361 AAGCAAAAGA GCGTCTAATG GCCTTGAGTC ATGAGAATGA GAAATTGAAG GAAGAGCTTG
            421 GAAAACTAAA AGGGAAATCA GAAAGGTCAT CTGAGGACCC CACTGATGAC TCCAGGCTTC
            481 CCAGGGCCGA AGCGGAGCAG GAAAAGGACC AGCTCAGGAC CCAGGTGGTG AGGCTACAAG
            541 CAGAGAAGGC AGACCTGTTG GGCATCGTGT CTGAACTGCA GCTCAAGCTG AACTCCAGCG
            601 GCTCCTCAGA AGATTCCTTT GTTGAAATTA GGATGGCTGA AGGAGAAGCA GAAGGGTCAG
            661 TAAAAGAAAT CAAGCATAGT CCTGGGCCCA CGAGAACAGT CTCCACTGGC ACGGCATTGT
            721 CTAAATATAG GAGCAGATCT GCAGATGGGG CCAAGAATTA CTTCGAACAT GAGGAGTTAA
            781 CTGTGAGCCA GCTCCTGCTG TGCCTAAGGG AAGGGAATCA GAAGGTGGAG AGACTTGAAG
            841 TTGCACTCAA GGAGGCCAAA GAAAGAGTTT CAGATTTTGA AAAGAAAACA AGTAATCGTT
            901 CTGAGATTGA AACCCAGACA GAGGGGAGCA CAGAGAAAGA GAATGATGAA GAGAAAGGCC
            961 CGGGAGACTG TTGGGAAGCG AAGTGAAGCA CTGAACCCTC CCAGGTGACA TCTCTGTTTA
           1021 GAGCTTTCAG GAGGCTCATA CAAACTCAGC GAAGCTGAAG CCTATGGAGG AGAGACTTCC
          1081 AAGATAGTGT CAGGTCCGTG CAAGAAAACT TCCTGCCATT TCCCATTCAG GAG
          //
          Primer: SV40polyA-R
          Sequence file: RDB16708_A8Ilb.seq check
          >D05635A2-1_A8Il_SV40polyA-R_E01_13_ABI24.ab1
              1 AAGAAGGGGG AAGTTTTTTA AAGCAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
             61 TCAGTTATCT AGATCCGGTG GATCCCGAAT GATGCAATCC ATCACGTGAA TCTGTAACGT
            121 GTCTATGTCA GGCAGAACCT CTCCACACTT GGGGCAGGAA TGAATCGGAA TATTCCGCTG
            181 TTGCCGCCAG TCCCTGTCCT CAGCTCCTCT TTGAACAAGG TAAGCCTGCT GGTCAGAGTC
            241 ACTTGTTCTC GCCCCATGAC GACTCTGCAT CTCCATCAAG GACTGCCTGC CTCCGTCTTC
            301 GAAAGCATCA TTCTCTTTCA GCAGAACTGC CAGCTGCAAT GCCAGTTGCT CCTTTTCCTC
            361 ATGAATTTTC TCTCTCGCTG CTCTTTCAGC ATGAAAATCA GAACAGTAAA CTTCCATCTG
            421 AGCCCTGAGG ATGGTCATGG TTTCCAGGTC CTCTTCCTGC TTGGCAATGG TTTGCTTCAT
            481 TTCATCCATT TGCAGCTGTT TGGAAGCCAG AGCCTTCTCT GCCAGTTCCA GTTTTTCACT
            541 CAGTTCCTTC AGCACTGCCC TGTCCACTTT TTCTGACTCT TTTCTTGTTA GTTCCTCAAT
            601 TGTTTTCAAT GCATTATTAT GTTCTTGAAG AAGCTTGTTG TGTGTCATCT GTAGCACAGT
            661 TAATTTGGAC TTTTCATCCT CTGTTTTAGC CTGTTCCATT TTGATTTCTG ATAGCATGCT
            721 TTCCACTTGT AGCTCTAACT TTTTGTTAGT ATAAACAAGC TCTTGCTTTT CATTCAACTC
            781 TGATGGAATT GCAGAATTTT TCCTTTCAAG GGCCTGACAC TTTTCTTGAA GTCTCTTCTT
            841 CATTAGCTCA GCTTCGCTGA GTTTTGTATG AGCCTCTTGA AGCTCCTTAA ACAGAGATGT
            901 CACCTGGAGG TTCAGTGCTT CCACTTCGCT TCCAACAGTC TCCGGGCCTT TCTCTTCATC
            961 ATTCTCTTTC TCTGTGCTCC CCTCTGTCTG GGTTTCAATC TCAGAACGAT TACTTTGTTT
           1021 TCTTTCAAAA TCTGAAACTC TTCTTTGGGC CTCTTGAGTG CAACTTTCAA GTCTCTCCAC
           1081 CTTCTGATCC TTTCCTTAGG CAACAGCAGG AAGCTGTCTC ACAGTAACTT CCTCATGATC
          1141 CGAAGGTATT CTTTGGGCCC CAATCTGTCA GAGAAATCCT G
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB16708_A8Ilc.seq check
          >D05635A2-1_A8Il_SV40pro_F_V2_F01_16_ABI24.ab1
              1 AAAGGAGGTT TTTTTGGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG
             61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
            601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
            781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
            841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
            901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGG
            961 CTTCGGAATC GTTTTCCGGG ACGCCCGGCT GGATGATCCC TCCAGCGCGG GGGATCTCAT
           1021 GCTGGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGAAGA GAACATACCG
           1081 GGAAGGACCC GCGCTATGAC GGGCATAAAA GACGGATAAC GCCACGTGTG GGTTCGGATT
          1141 GTCATTAAAC GCCGGGATTC CGGTTCCCCA AG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl