Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GFP-OPTN-E50K (#RDB16708)

Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant.

Clone info. Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16708 GFP-OPTN-E50K DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GFP-OPTN-E50K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16708).

Reference section:

Kitamura, A., Shimizu, H., Kinjo, M., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16708_A8Ilp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: VenusC_F2 ()
Sequence file: RDB16708_A8Ila.seq check
>D05635A2_A8Il__1_VenusC_F2_B01_02_ABI08.ab1
    1 GGGAGTGGTA AAGCGCCGGG AACACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
   61 GATCTCGAGC TCAAGCTTCG AATTCTATGT CCCATCAACC TCTCAGCTGC CTCACTGAAA
  121 AGGAGGACAG CCCCAGTGAA AGCACAGGAA ATGGACCCCC CCACCTGGCC CACCCAAACC
  181 TGGACACGTT TACCCCGGAG GAGCTGCTGC AGCAGATGAA AGAGCTCCTG ACCAAGAACC
  241 ACCAGCTGAA AGAAGCCATG AAGCTAAATA ATCAAGCCAT GAAAGGGAGA TTTGAGGAGC
  301 TTTCGGCCTG GACAGAGAAA CAGAAGGAAG AACGCCAGTT TTTTGAGATA CAGAGCAAAG
  361 AAGCAAAAGA GCGTCTAATG GCCTTGAGTC ATGAGAATGA GAAATTGAAG GAAGAGCTTG
  421 GAAAACTAAA AGGGAAATCA GAAAGGTCAT CTGAGGACCC CACTGATGAC TCCAGGCTTC
  481 CCAGGGCCGA AGCGGAGCAG GAAAAGGACC AGCTCAGGAC CCAGGTGGTG AGGCTACAAG
  541 CAGAGAAGGC AGACCTGTTG GGCATCGTGT CTGAACTGCA GCTCAAGCTG AACTCCAGCG
  601 GCTCCTCAGA AGATTCCTTT GTTGAAATTA GGATGGCTGA AGGAGAAGCA GAAGGGTCAG
  661 TAAAAGAAAT CAAGCATAGT CCTGGGCCCA CGAGAACAGT CTCCACTGGC ACGGCATTGT
  721 CTAAATATAG GAGCAGATCT GCAGATGGGG CCAAGAATTA CTTCGAACAT GAGGAGTTAA
  781 CTGTGAGCCA GCTCCTGCTG TGCCTAAGGG AAGGGAATCA GAAGGTGGAG AGACTTGAAG
  841 TTGCACTCAA GGAGGCCAAA GAAAGAGTTT CAGATTTTGA AAAGAAAACA AGTAATCGTT
  901 CTGAGATTGA AACCCAGACA GAGGGGAGCA CAGAGAAAGA GAATGATGAA GAGAAAGGCC
  961 CGGGAGACTG TTGGGAAGCG AAGTGAAGCA CTGAACCCTC CCAGGTGACA TCTCTGTTTA
 1021 GAGCTTTCAG GAGGCTCATA CAAACTCAGC GAAGCTGAAG CCTATGGAGG AGAGACTTCC
1081 AAGATAGTGT CAGGTCCGTG CAAGAAAACT TCCTGCCATT TCCCATTCAG GAG
//
Primer: SV40polyA-R ()
Sequence file: RDB16708_A8Ilb.seq check
>D05635A2-1_A8Il_SV40polyA-R_E01_13_ABI24.ab1
    1 AAGAAGGGGG AAGTTTTTTA AAGCAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
   61 TCAGTTATCT AGATCCGGTG GATCCCGAAT GATGCAATCC ATCACGTGAA TCTGTAACGT
  121 GTCTATGTCA GGCAGAACCT CTCCACACTT GGGGCAGGAA TGAATCGGAA TATTCCGCTG
  181 TTGCCGCCAG TCCCTGTCCT CAGCTCCTCT TTGAACAAGG TAAGCCTGCT GGTCAGAGTC
  241 ACTTGTTCTC GCCCCATGAC GACTCTGCAT CTCCATCAAG GACTGCCTGC CTCCGTCTTC
  301 GAAAGCATCA TTCTCTTTCA GCAGAACTGC CAGCTGCAAT GCCAGTTGCT CCTTTTCCTC
  361 ATGAATTTTC TCTCTCGCTG CTCTTTCAGC ATGAAAATCA GAACAGTAAA CTTCCATCTG
  421 AGCCCTGAGG ATGGTCATGG TTTCCAGGTC CTCTTCCTGC TTGGCAATGG TTTGCTTCAT
  481 TTCATCCATT TGCAGCTGTT TGGAAGCCAG AGCCTTCTCT GCCAGTTCCA GTTTTTCACT
  541 CAGTTCCTTC AGCACTGCCC TGTCCACTTT TTCTGACTCT TTTCTTGTTA GTTCCTCAAT
  601 TGTTTTCAAT GCATTATTAT GTTCTTGAAG AAGCTTGTTG TGTGTCATCT GTAGCACAGT
  661 TAATTTGGAC TTTTCATCCT CTGTTTTAGC CTGTTCCATT TTGATTTCTG ATAGCATGCT
  721 TTCCACTTGT AGCTCTAACT TTTTGTTAGT ATAAACAAGC TCTTGCTTTT CATTCAACTC
  781 TGATGGAATT GCAGAATTTT TCCTTTCAAG GGCCTGACAC TTTTCTTGAA GTCTCTTCTT
  841 CATTAGCTCA GCTTCGCTGA GTTTTGTATG AGCCTCTTGA AGCTCCTTAA ACAGAGATGT
  901 CACCTGGAGG TTCAGTGCTT CCACTTCGCT TCCAACAGTC TCCGGGCCTT TCTCTTCATC
  961 ATTCTCTTTC TCTGTGCTCC CCTCTGTCTG GGTTTCAATC TCAGAACGAT TACTTTGTTT
 1021 TCTTTCAAAA TCTGAAACTC TTCTTTGGGC CTCTTGAGTG CAACTTTCAA GTCTCTCCAC
 1081 CTTCTGATCC TTTCCTTAGG CAACAGCAGG AAGCTGTCTC ACAGTAACTT CCTCATGATC
1141 CGAAGGTATT CTTTGGGCCC CAATCTGTCA GAGAAATCCT G
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB16708_A8Ilc.seq check
>D05635A2-1_A8Il_SV40pro_F_V2_F01_16_ABI24.ab1
    1 AAAGGAGGTT TTTTTGGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG
   61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGG
  961 CTTCGGAATC GTTTTCCGGG ACGCCCGGCT GGATGATCCC TCCAGCGCGG GGGATCTCAT
 1021 GCTGGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGAAGA GAACATACCG
 1081 GGAAGGACCC GCGCTATGAC GGGCATAAAA GACGGATAAC GCCACGTGTG GGTTCGGATT
1141 GTCATTAAAC GCCGGGATTC CGGTTCCCCA AG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153. [link to RRC of NBRP]